This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 62.72 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 102,812 (4.2%) Reads that were too short: 38,650 (1.6%) Reads written (passing filters): 64,162 (2.6%) Total basepairs processed: 735,190,694 bp Total written (filtered): 12,896,204 bp (1.8%) === Adapter 1 === Sequence: TCCGCT; Type: regular 5'; Length: 6; Trimmed: 102812 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 273 596.3 0 273 7 594 596.3 0 594 8 8084 596.3 0 8084 9 10194 596.3 0 10194 10 15 596.3 0 15 11 11 596.3 0 11 12 6 596.3 0 6 13 7 596.3 0 7 14 8 596.3 0 8 15 11 596.3 0 11 16 4 596.3 0 4 17 13 596.3 0 13 18 4 596.3 0 4 19 7 596.3 0 7 20 4 596.3 0 4 21 4 596.3 0 4 22 15 596.3 0 15 23 10 596.3 0 10 24 12 596.3 0 12 25 8 596.3 0 8 26 6 596.3 0 6 27 10 596.3 0 10 28 6 596.3 0 6 29 9 596.3 0 9 30 3 596.3 0 3 31 11 596.3 0 11 32 145 596.3 0 145 33 29 596.3 0 29 34 38 596.3 0 38 35 24 596.3 0 24 36 24 596.3 0 24 37 144 596.3 0 144 38 101 596.3 0 101 39 201 596.3 0 201 40 28 596.3 0 28 41 19 596.3 0 19 42 17 596.3 0 17 43 10 596.3 0 10 44 11 596.3 0 11 45 10 596.3 0 10 46 14 596.3 0 14 47 13 596.3 0 13 48 18 596.3 0 18 49 13 596.3 0 13 50 14 596.3 0 14 51 12 596.3 0 12 52 158 596.3 0 158 53 79 596.3 0 79 54 215 596.3 0 215 55 14 596.3 0 14 56 12 596.3 0 12 57 6 596.3 0 6 58 12 596.3 0 12 59 11 596.3 0 11 60 23 596.3 0 23 61 24 596.3 0 24 62 724 596.3 0 724 63 103 596.3 0 103 64 15 596.3 0 15 65 9 596.3 0 9 66 17 596.3 0 17 67 13 596.3 0 13 68 13 596.3 0 13 69 6 596.3 0 6 70 12 596.3 0 12 71 22 596.3 0 22 72 42 596.3 0 42 73 42 596.3 0 42 74 9 596.3 0 9 75 8 596.3 0 8 76 8 596.3 0 8 77 11 596.3 0 11 78 8 596.3 0 8 79 6 596.3 0 6 80 9 596.3 0 9 81 15 596.3 0 15 82 16 596.3 0 16 83 14 596.3 0 14 84 65 596.3 0 65 85 66 596.3 0 66 86 104 596.3 0 104 87 14 596.3 0 14 88 14 596.3 0 14 89 16 596.3 0 16 90 41 596.3 0 41 91 33 596.3 0 33 92 47 596.3 0 47 93 25 596.3 0 25 94 26 596.3 0 26 95 65 596.3 0 65 96 19 596.3 0 19 97 44 596.3 0 44 98 11 596.3 0 11 99 12 596.3 0 12 100 21 596.3 0 21 101 11 596.3 0 11 102 7 596.3 0 7 103 18 596.3 0 18 104 47 596.3 0 47 105 43 596.3 0 43 106 37 596.3 0 37 107 44 596.3 0 44 108 67 596.3 0 67 109 68 596.3 0 68 110 83 596.3 0 83 111 61 596.3 0 61 112 94 596.3 0 94 113 45 596.3 0 45 114 32 596.3 0 32 115 30 596.3 0 30 116 14 596.3 0 14 117 102 596.3 0 102 118 29 596.3 0 29 119 58 596.3 0 58 120 45 596.3 0 45 121 10 596.3 0 10 122 8 596.3 0 8 123 14 596.3 0 14 124 30 596.3 0 30 125 24 596.3 0 24 126 54 596.3 0 54 127 329 596.3 0 329 128 102 596.3 0 102 129 259 596.3 0 259 130 46 596.3 0 46 131 59 596.3 0 59 132 161 596.3 0 161 133 57 596.3 0 57 134 67 596.3 0 67 135 30 596.3 0 30 136 23 596.3 0 23 137 53 596.3 0 53 138 55 596.3 0 55 139 125 596.3 0 125 140 212 596.3 0 212 141 241 596.3 0 241 142 381 596.3 0 381 143 882 596.3 0 882 144 14209 596.3 0 14209 145 9213 596.3 0 9213 146 11379 596.3 0 11379 147 100 596.3 0 100 148 90 596.3 0 90 149 21 596.3 0 21 150 10 596.3 0 10 151 81 596.3 0 81 152 53 596.3 0 53 153 89 596.3 0 89 154 47 596.3 0 47 155 53 596.3 0 53 156 60 596.3 0 60 157 141 596.3 0 141 158 115 596.3 0 115 159 25 596.3 0 25 160 27 596.3 0 27 161 21 596.3 0 21 162 14 596.3 0 14 163 10 596.3 0 10 164 7 596.3 0 7 165 17 596.3 0 17 166 16 596.3 0 16 167 12 596.3 0 12 168 8 596.3 0 8 169 21 596.3 0 21 170 25 596.3 0 25 171 25 596.3 0 25 172 49 596.3 0 49 173 19 596.3 0 19 174 14 596.3 0 14 175 25 596.3 0 25 176 11 596.3 0 11 177 29 596.3 0 29 178 38 596.3 0 38 179 193 596.3 0 193 180 38 596.3 0 38 181 100 596.3 0 100 182 19 596.3 0 19 183 17 596.3 0 17 184 16 596.3 0 16 185 21 596.3 0 21 186 24 596.3 0 24 187 13 596.3 0 13 188 17 596.3 0 17 189 28 596.3 0 28 190 280 596.3 0 280 191 111 596.3 0 111 192 263 596.3 0 263 193 25 596.3 0 25 194 20 596.3 0 20 195 15 596.3 0 15 196 13 596.3 0 13 197 34 596.3 0 34 198 66 596.3 0 66 199 53 596.3 0 53 200 33 596.3 0 33 201 19 596.3 0 19 202 12 596.3 0 12 203 20 596.3 0 20 204 16 596.3 0 16 205 19 596.3 0 19 206 17 596.3 0 17 207 21 596.3 0 21 208 14 596.3 0 14 209 18 596.3 0 18 210 11 596.3 0 11 211 10 596.3 0 10 212 15 596.3 0 15 213 25 596.3 0 25 214 19 596.3 0 19 215 23 596.3 0 23 216 21 596.3 0 21 217 139 596.3 0 139 218 61 596.3 0 61 219 838 596.3 0 838 220 22 596.3 0 22 221 16 596.3 0 16 222 15 596.3 0 15 223 19 596.3 0 19 224 10 596.3 0 10 225 36 596.3 0 36 226 26 596.3 0 26 227 38 596.3 0 38 228 37 596.3 0 37 229 51 596.3 0 51 230 128 596.3 0 128 231 101 596.3 0 101 232 146 596.3 0 146 233 29 596.3 0 29 234 35 596.3 0 35 235 123 596.3 0 123 236 273 596.3 0 273 237 245 596.3 0 245 238 271 596.3 0 271 239 56 596.3 0 56 240 462 596.3 0 462 241 183 596.3 0 183 242 422 596.3 0 422 243 32 596.3 0 32 244 27 596.3 0 27 245 41 596.3 0 41 246 45 596.3 0 45 247 104 596.3 0 104 248 79 596.3 0 79 249 67 596.3 0 67 250 102 596.3 0 102 251 236 596.3 0 236 252 255 596.3 0 255 253 42 596.3 0 42 254 29 596.3 0 29 255 33 596.3 0 33 256 49 596.3 0 49 257 65 596.3 0 65 258 128 596.3 0 128 259 267 596.3 0 267 260 3192 596.3 0 3192 261 1793 596.3 0 1793 262 2541 596.3 0 2541 263 807 596.3 0 807 264 29 596.3 0 29 265 32 596.3 0 32 266 59 596.3 0 59 267 78 596.3 0 78 268 92 596.3 0 92 269 96 596.3 0 96 270 220 596.3 0 220 271 429 596.3 0 429 272 235 596.3 0 235 273 141 596.3 0 141 274 74 596.3 0 74 275 58 596.3 0 58 276 149 596.3 0 149 277 161 596.3 0 161 278 164 596.3 0 164 279 237 596.3 0 237 280 265 596.3 0 265 281 361 596.3 0 361 282 380 596.3 0 380 283 3748 596.3 0 3748 284 13178 596.3 0 13178 285 2878 596.3 0 2878 286 37 596.3 0 37 287 58 596.3 0 58 288 182 596.3 0 182 289 133 596.3 0 133 290 99 596.3 0 99 291 153 596.3 0 153 292 127 596.3 0 127 293 226 596.3 0 226 294 148 596.3 0 148 295 121 596.3 0 121 296 149 596.3 0 149 297 35 596.3 0 35 298 24 596.3 0 24 299 30 596.3 0 30 300 28 596.3 0 28 301 59 596.3 0 59 Finished in 2.71 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 64,162 Reads with adapters: 10,797 (16.8%) Reads that were too short: 3 (0.0%) Reads written (passing filters): 10,794 (16.8%) Total basepairs processed: 12,896,204 bp Total written (filtered): 2,927,662 bp (22.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 10797 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 3 19 176 0.0 3 0 169 5 2 20 10357 0.0 4 10147 201 8 0 1 21 19 0.0 4 2 17 22 1 0.0 4 0 1 23 151 0.0 4 147 1 0 3 24 1 0.0 4 0 1 48 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 81 0.0 4 0 0 0 0 81 140 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.38 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 10,794 Reads with adapters: 1,205 (11.2%) Reads written (passing filters): 10,794 (100.0%) Total basepairs processed: 2,927,662 bp Total written (filtered): 2,915,897 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1205 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.4% C: 4.0% G: 92.6% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 168.7 0 2 4 2 42.2 0 2 5 1 10.5 0 1 8 2 0.2 0 2 9 343 0.0 0 8 335 10 765 0.0 1 27 738 11 88 0.0 1 1 87 12 1 0.0 1 0 1 13 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.