This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.14 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 209,036 (8.6%) Reads that were too short: 12,293 (0.5%) Reads written (passing filters): 196,743 (8.1%) Total basepairs processed: 735,190,694 bp Total written (filtered): 55,700,792 bp (7.6%) === Adapter 1 === Sequence: ACAGGT; Type: regular 5'; Length: 6; Trimmed: 209036 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1801 596.3 0 1801 7 96961 596.3 0 96961 8 78264 596.3 0 78264 9 83 596.3 0 83 10 47 596.3 0 47 11 31 596.3 0 31 12 188 596.3 0 188 13 15 596.3 0 15 14 28 596.3 0 28 15 19 596.3 0 19 16 26 596.3 0 26 17 32 596.3 0 32 18 36 596.3 0 36 19 28 596.3 0 28 20 134 596.3 0 134 21 26 596.3 0 26 22 24 596.3 0 24 23 21 596.3 0 21 24 19 596.3 0 19 25 33 596.3 0 33 26 34 596.3 0 34 27 37 596.3 0 37 28 18 596.3 0 18 29 26 596.3 0 26 30 46 596.3 0 46 31 26 596.3 0 26 32 27 596.3 0 27 33 25 596.3 0 25 34 37 596.3 0 37 35 30 596.3 0 30 36 27 596.3 0 27 37 37 596.3 0 37 38 36 596.3 0 36 39 41 596.3 0 41 40 21 596.3 0 21 41 41 596.3 0 41 42 35 596.3 0 35 43 22 596.3 0 22 44 43 596.3 0 43 45 39 596.3 0 39 46 25 596.3 0 25 47 26 596.3 0 26 48 46 596.3 0 46 49 23 596.3 0 23 50 35 596.3 0 35 51 22 596.3 0 22 52 21 596.3 0 21 53 38 596.3 0 38 54 52 596.3 0 52 55 40 596.3 0 40 56 31 596.3 0 31 57 24 596.3 0 24 58 27 596.3 0 27 59 42 596.3 0 42 60 27 596.3 0 27 61 34 596.3 0 34 62 34 596.3 0 34 63 30 596.3 0 30 64 35 596.3 0 35 65 51 596.3 0 51 66 60 596.3 0 60 67 74 596.3 0 74 68 90 596.3 0 90 69 112 596.3 0 112 70 173 596.3 0 173 71 2198 596.3 0 2198 72 756 596.3 0 756 73 2078 596.3 0 2078 74 55 596.3 0 55 75 27 596.3 0 27 76 47 596.3 0 47 77 124 596.3 0 124 78 60 596.3 0 60 79 147 596.3 0 147 80 30 596.3 0 30 81 23 596.3 0 23 82 28 596.3 0 28 83 57 596.3 0 57 84 58 596.3 0 58 85 56 596.3 0 56 86 89 596.3 0 89 87 63 596.3 0 63 88 28 596.3 0 28 89 22 596.3 0 22 90 30 596.3 0 30 91 39 596.3 0 39 92 38 596.3 0 38 93 42 596.3 0 42 94 65 596.3 0 65 95 88 596.3 0 88 96 883 596.3 0 883 97 346 596.3 0 346 98 677 596.3 0 677 99 248 596.3 0 248 100 33 596.3 0 33 101 35 596.3 0 35 102 35 596.3 0 35 103 60 596.3 0 60 104 39 596.3 0 39 105 27 596.3 0 27 106 30 596.3 0 30 107 29 596.3 0 29 108 31 596.3 0 31 109 30 596.3 0 30 110 23 596.3 0 23 111 25 596.3 0 25 112 41 596.3 0 41 113 35 596.3 0 35 114 43 596.3 0 43 115 49 596.3 0 49 116 55 596.3 0 55 117 62 596.3 0 62 118 94 596.3 0 94 119 52 596.3 0 52 120 31 596.3 0 31 121 29 596.3 0 29 122 44 596.3 0 44 123 47 596.3 0 47 124 88 596.3 0 88 125 120 596.3 0 120 126 136 596.3 0 136 127 71 596.3 0 71 128 76 596.3 0 76 129 120 596.3 0 120 130 111 596.3 0 111 131 55 596.3 0 55 132 156 596.3 0 156 133 55 596.3 0 55 134 54 596.3 0 54 135 118 596.3 0 118 136 55 596.3 0 55 137 68 596.3 0 68 138 39 596.3 0 39 139 53 596.3 0 53 140 60 596.3 0 60 141 74 596.3 0 74 142 65 596.3 0 65 143 74 596.3 0 74 144 77 596.3 0 77 145 134 596.3 0 134 146 70 596.3 0 70 147 64 596.3 0 64 148 54 596.3 0 54 149 86 596.3 0 86 150 63 596.3 0 63 151 62 596.3 0 62 152 39 596.3 0 39 153 41 596.3 0 41 154 51 596.3 0 51 155 62 596.3 0 62 156 58 596.3 0 58 157 38 596.3 0 38 158 42 596.3 0 42 159 32 596.3 0 32 160 42 596.3 0 42 161 48 596.3 0 48 162 40 596.3 0 40 163 34 596.3 0 34 164 53 596.3 0 53 165 68 596.3 0 68 166 55 596.3 0 55 167 63 596.3 0 63 168 31 596.3 0 31 169 43 596.3 0 43 170 36 596.3 0 36 171 46 596.3 0 46 172 89 596.3 0 89 173 231 596.3 0 231 174 150 596.3 0 150 175 320 596.3 0 320 176 79 596.3 0 79 177 40 596.3 0 40 178 49 596.3 0 49 179 56 596.3 0 56 180 64 596.3 0 64 181 56 596.3 0 56 182 47 596.3 0 47 183 49 596.3 0 49 184 40 596.3 0 40 185 76 596.3 0 76 186 92 596.3 0 92 187 150 596.3 0 150 188 72 596.3 0 72 189 59 596.3 0 59 190 78 596.3 0 78 191 156 596.3 0 156 192 696 596.3 0 696 193 464 596.3 0 464 194 749 596.3 0 749 195 343 596.3 0 343 196 50 596.3 0 50 197 47 596.3 0 47 198 44 596.3 0 44 199 79 596.3 0 79 200 80 596.3 0 80 201 41 596.3 0 41 202 55 596.3 0 55 203 66 596.3 0 66 204 61 596.3 0 61 205 82 596.3 0 82 206 125 596.3 0 125 207 93 596.3 0 93 208 87 596.3 0 87 209 76 596.3 0 76 210 144 596.3 0 144 211 140 596.3 0 140 212 135 596.3 0 135 213 70 596.3 0 70 214 43 596.3 0 43 215 62 596.3 0 62 216 59 596.3 0 59 217 143 596.3 0 143 218 68 596.3 0 68 219 99 596.3 0 99 220 148 596.3 0 148 221 99 596.3 0 99 222 59 596.3 0 59 223 44 596.3 0 44 224 41 596.3 0 41 225 130 596.3 0 130 226 101 596.3 0 101 227 324 596.3 0 324 228 85 596.3 0 85 229 75 596.3 0 75 230 59 596.3 0 59 231 45 596.3 0 45 232 54 596.3 0 54 233 81 596.3 0 81 234 75 596.3 0 75 235 91 596.3 0 91 236 92 596.3 0 92 237 127 596.3 0 127 238 122 596.3 0 122 239 87 596.3 0 87 240 128 596.3 0 128 241 200 596.3 0 200 242 152 596.3 0 152 243 99 596.3 0 99 244 67 596.3 0 67 245 78 596.3 0 78 246 95 596.3 0 95 247 125 596.3 0 125 248 148 596.3 0 148 249 174 596.3 0 174 250 75 596.3 0 75 251 72 596.3 0 72 252 60 596.3 0 60 253 80 596.3 0 80 254 165 596.3 0 165 255 190 596.3 0 190 256 118 596.3 0 118 257 87 596.3 0 87 258 102 596.3 0 102 259 106 596.3 0 106 260 98 596.3 0 98 261 106 596.3 0 106 262 106 596.3 0 106 263 77 596.3 0 77 264 86 596.3 0 86 265 184 596.3 0 184 266 366 596.3 0 366 267 162 596.3 0 162 268 148 596.3 0 148 269 96 596.3 0 96 270 135 596.3 0 135 271 159 596.3 0 159 272 383 596.3 0 383 273 493 596.3 0 493 274 411 596.3 0 411 275 189 596.3 0 189 276 89 596.3 0 89 277 79 596.3 0 79 278 107 596.3 0 107 279 123 596.3 0 123 280 157 596.3 0 157 281 170 596.3 0 170 282 135 596.3 0 135 283 90 596.3 0 90 284 53 596.3 0 53 285 84 596.3 0 84 286 123 596.3 0 123 287 91 596.3 0 91 288 154 596.3 0 154 289 137 596.3 0 137 290 240 596.3 0 240 291 131 596.3 0 131 292 95 596.3 0 95 293 125 596.3 0 125 294 209 596.3 0 209 295 190 596.3 0 190 296 225 596.3 0 225 297 87 596.3 0 87 298 75 596.3 0 75 299 98 596.3 0 98 300 105 596.3 0 105 301 54 596.3 0 54 Finished in 8.84 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 196,743 Reads with adapters: 96,380 (49.0%) Reads that were too short: 159 (0.1%) Reads written (passing filters): 96,221 (48.9%) Total basepairs processed: 55,700,792 bp Total written (filtered): 26,641,834 bp (47.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 96380 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 15 0.0 2 0 0 0 15 15 55 0.0 3 0 0 28 27 16 711 0.0 3 0 582 86 43 17 94597 0.0 3 88961 4712 743 181 18 187 0.0 3 15 137 20 15 19 324 0.0 3 0 0 208 116 20 258 0.0 3 1 1 0 256 21 2 0.0 3 2 22 2 0.0 3 0 0 0 2 25 2 0.0 3 1 0 0 1 28 2 0.0 3 0 0 0 2 29 2 0.0 3 0 0 0 2 30 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 1 37 1 0.0 3 0 0 0 1 38 2 0.0 3 0 0 1 1 41 1 0.0 3 0 0 0 1 42 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 0 1 44 7 0.0 3 0 0 0 7 46 2 0.0 3 0 0 0 2 47 3 0.0 3 0 0 0 3 48 3 0.0 3 0 0 0 3 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 53 3 0.0 3 0 0 0 3 54 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 67 4 0.0 3 0 0 0 4 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 1 71 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 2 0.0 3 0 0 1 1 81 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 3 0.0 3 0 0 0 3 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 102 3 0.0 3 0 0 0 3 107 2 0.0 3 0 0 0 2 108 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 114 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 124 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 140 2 0.0 3 0 0 0 2 147 2 0.0 3 0 0 0 2 148 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 156 2 0.0 3 0 0 0 2 157 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 164 6 0.0 3 0 0 0 6 165 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 12 0.0 3 0 0 0 12 203 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 210 2 0.0 3 0 0 0 2 216 1 0.0 3 0 0 0 1 217 5 0.0 3 0 0 1 4 218 1 0.0 3 0 0 0 1 221 27 0.0 3 0 0 1 26 231 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 270 5 0.0 3 0 0 0 5 278 1 0.0 3 0 0 0 1 279 10 0.0 3 0 0 0 10 280 6 0.0 3 0 0 0 6 281 20 0.0 3 0 0 1 19 282 8 0.0 3 0 0 0 8 285 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 288 2 0.0 3 0 0 0 2 289 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.40 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 96,221 Reads with adapters: 921 (1.0%) Reads written (passing filters): 96,221 (100.0%) Total basepairs processed: 26,641,834 bp Total written (filtered): 26,638,993 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 921 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 18.5% C: 59.4% G: 14.7% T: 7.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 889 1503.5 0 889 4 19 375.9 0 19 5 7 94.0 0 7 6 1 23.5 0 1 9 1 0.4 0 0 1 12 4 0.0 1 0 4