This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCTGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.28 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 173,767 (7.1%) Reads that were too short: 24,743 (1.0%) Reads written (passing filters): 149,024 (6.1%) Total basepairs processed: 735,190,694 bp Total written (filtered): 28,490,668 bp (3.9%) === Adapter 1 === Sequence: CTCTGC; Type: regular 5'; Length: 6; Trimmed: 173767 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 393 596.3 0 393 7 11233 596.3 0 11233 8 335 596.3 0 335 9 9646 596.3 0 9646 10 24 596.3 0 24 11 21 596.3 0 21 12 20 596.3 0 20 13 21 596.3 0 21 14 122 596.3 0 122 15 29 596.3 0 29 16 52 596.3 0 52 17 13 596.3 0 13 18 13 596.3 0 13 19 24 596.3 0 24 20 17 596.3 0 17 21 21 596.3 0 21 22 23 596.3 0 23 23 29 596.3 0 29 24 39 596.3 0 39 25 34 596.3 0 34 26 21 596.3 0 21 27 30 596.3 0 30 28 19 596.3 0 19 29 19 596.3 0 19 30 26 596.3 0 26 31 27 596.3 0 27 32 34 596.3 0 34 33 39 596.3 0 39 34 24 596.3 0 24 35 30 596.3 0 30 36 47 596.3 0 47 37 27 596.3 0 27 38 36 596.3 0 36 39 117 596.3 0 117 40 56 596.3 0 56 41 36 596.3 0 36 42 22 596.3 0 22 43 19 596.3 0 19 44 24 596.3 0 24 45 31 596.3 0 31 46 47 596.3 0 47 47 33 596.3 0 33 48 41 596.3 0 41 49 27 596.3 0 27 50 19 596.3 0 19 51 41 596.3 0 41 52 28 596.3 0 28 53 34 596.3 0 34 54 33 596.3 0 33 55 45 596.3 0 45 56 135 596.3 0 135 57 169 596.3 0 169 58 229 596.3 0 229 59 311 596.3 0 311 60 86 596.3 0 86 61 244 596.3 0 244 62 23 596.3 0 23 63 38 596.3 0 38 64 38 596.3 0 38 65 24 596.3 0 24 66 19 596.3 0 19 67 36 596.3 0 36 68 24 596.3 0 24 69 51 596.3 0 51 70 52 596.3 0 52 71 69 596.3 0 69 72 97 596.3 0 97 73 40 596.3 0 40 74 44 596.3 0 44 75 33 596.3 0 33 76 51 596.3 0 51 77 132 596.3 0 132 78 51 596.3 0 51 79 38 596.3 0 38 80 30 596.3 0 30 81 37 596.3 0 37 82 47 596.3 0 47 83 63 596.3 0 63 84 72 596.3 0 72 85 89 596.3 0 89 86 59 596.3 0 59 87 146 596.3 0 146 88 47 596.3 0 47 89 85 596.3 0 85 90 65 596.3 0 65 91 119 596.3 0 119 92 72 596.3 0 72 93 71 596.3 0 71 94 281 596.3 0 281 95 69 596.3 0 69 96 102 596.3 0 102 97 250 596.3 0 250 98 80 596.3 0 80 99 109 596.3 0 109 100 65 596.3 0 65 101 81 596.3 0 81 102 180 596.3 0 180 103 93 596.3 0 93 104 98 596.3 0 98 105 92 596.3 0 92 106 359 596.3 0 359 107 354 596.3 0 354 108 462 596.3 0 462 109 326 596.3 0 326 110 434 596.3 0 434 111 453 596.3 0 453 112 927 596.3 0 927 113 1779 596.3 0 1779 114 23978 596.3 0 23978 115 12436 596.3 0 12436 116 32642 596.3 0 32642 117 277 596.3 0 277 118 218 596.3 0 218 119 34 596.3 0 34 120 38 596.3 0 38 121 25 596.3 0 25 122 26 596.3 0 26 123 33 596.3 0 33 124 40 596.3 0 40 125 30 596.3 0 30 126 116 596.3 0 116 127 158 596.3 0 158 128 166 596.3 0 166 129 167 596.3 0 167 130 137 596.3 0 137 131 136 596.3 0 136 132 53 596.3 0 53 133 33 596.3 0 33 134 45 596.3 0 45 135 97 596.3 0 97 136 142 596.3 0 142 137 92 596.3 0 92 138 87 596.3 0 87 139 68 596.3 0 68 140 411 596.3 0 411 141 737 596.3 0 737 142 522 596.3 0 522 143 404 596.3 0 404 144 164 596.3 0 164 145 197 596.3 0 197 146 277 596.3 0 277 147 2513 596.3 0 2513 148 4609 596.3 0 4609 149 3554 596.3 0 3554 150 267 596.3 0 267 151 348 596.3 0 348 152 847 596.3 0 847 153 9219 596.3 0 9219 154 3388 596.3 0 3388 155 10451 596.3 0 10451 156 354 596.3 0 354 157 240 596.3 0 240 158 186 596.3 0 186 159 97 596.3 0 97 160 219 596.3 0 219 161 157 596.3 0 157 162 184 596.3 0 184 163 59 596.3 0 59 164 65 596.3 0 65 165 89 596.3 0 89 166 65 596.3 0 65 167 121 596.3 0 121 168 414 596.3 0 414 169 410 596.3 0 410 170 547 596.3 0 547 171 177 596.3 0 177 172 44 596.3 0 44 173 24 596.3 0 24 174 23 596.3 0 23 175 42 596.3 0 42 176 74 596.3 0 74 177 48 596.3 0 48 178 55 596.3 0 55 179 42 596.3 0 42 180 118 596.3 0 118 181 150 596.3 0 150 182 78 596.3 0 78 183 457 596.3 0 457 184 395 596.3 0 395 185 386 596.3 0 386 186 599 596.3 0 599 187 224 596.3 0 224 188 145 596.3 0 145 189 83 596.3 0 83 190 156 596.3 0 156 191 108 596.3 0 108 192 80 596.3 0 80 193 33 596.3 0 33 194 34 596.3 0 34 195 26 596.3 0 26 196 45 596.3 0 45 197 47 596.3 0 47 198 44 596.3 0 44 199 52 596.3 0 52 200 35 596.3 0 35 201 40 596.3 0 40 202 140 596.3 0 140 203 71 596.3 0 71 204 190 596.3 0 190 205 48 596.3 0 48 206 44 596.3 0 44 207 30 596.3 0 30 208 31 596.3 0 31 209 34 596.3 0 34 210 32 596.3 0 32 211 36 596.3 0 36 212 45 596.3 0 45 213 52 596.3 0 52 214 44 596.3 0 44 215 86 596.3 0 86 216 610 596.3 0 610 217 314 596.3 0 314 218 639 596.3 0 639 219 45 596.3 0 45 220 35 596.3 0 35 221 31 596.3 0 31 222 36 596.3 0 36 223 35 596.3 0 35 224 43 596.3 0 43 225 41 596.3 0 41 226 74 596.3 0 74 227 64 596.3 0 64 228 91 596.3 0 91 229 34 596.3 0 34 230 59 596.3 0 59 231 57 596.3 0 57 232 78 596.3 0 78 233 110 596.3 0 110 234 121 596.3 0 121 235 187 596.3 0 187 236 121 596.3 0 121 237 100 596.3 0 100 238 59 596.3 0 59 239 43 596.3 0 43 240 35 596.3 0 35 241 33 596.3 0 33 242 43 596.3 0 43 243 63 596.3 0 63 244 120 596.3 0 120 245 99 596.3 0 99 246 147 596.3 0 147 247 95 596.3 0 95 248 85 596.3 0 85 249 198 596.3 0 198 250 342 596.3 0 342 251 290 596.3 0 290 252 80 596.3 0 80 253 65 596.3 0 65 254 94 596.3 0 94 255 94 596.3 0 94 256 85 596.3 0 85 257 66 596.3 0 66 258 45 596.3 0 45 259 89 596.3 0 89 260 51 596.3 0 51 261 72 596.3 0 72 262 87 596.3 0 87 263 90 596.3 0 90 264 75 596.3 0 75 265 77 596.3 0 77 266 81 596.3 0 81 267 118 596.3 0 118 268 109 596.3 0 109 269 148 596.3 0 148 270 214 596.3 0 214 271 376 596.3 0 376 272 225 596.3 0 225 273 89 596.3 0 89 274 191 596.3 0 191 275 147 596.3 0 147 276 192 596.3 0 192 277 231 596.3 0 231 278 248 596.3 0 248 279 1031 596.3 0 1031 280 975 596.3 0 975 281 2559 596.3 0 2559 282 3545 596.3 0 3545 283 2680 596.3 0 2680 284 200 596.3 0 200 285 201 596.3 0 201 286 142 596.3 0 142 287 108 596.3 0 108 288 62 596.3 0 62 289 116 596.3 0 116 290 585 596.3 0 585 291 452 596.3 0 452 292 645 596.3 0 645 293 293 596.3 0 293 294 309 596.3 0 309 295 382 596.3 0 382 296 407 596.3 0 407 297 416 596.3 0 416 298 477 596.3 0 477 299 158 596.3 0 158 300 37 596.3 0 37 301 64 596.3 0 64 Finished in 5.41 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 149,024 Reads with adapters: 11,668 (7.8%) Reads that were too short: 193 (0.1%) Reads written (passing filters): 11,475 (7.7%) Total basepairs processed: 28,490,668 bp Total written (filtered): 3,150,502 bp (11.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 11668 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 0 3 16 118 0.0 3 0 100 10 8 17 11062 0.0 3 10332 588 108 34 18 36 0.0 3 1 25 2 8 19 47 0.0 3 0 0 27 20 20 41 0.0 3 0 0 1 40 21 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 32 5 0.0 3 0 0 0 5 34 2 0.0 3 0 0 0 2 38 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 45 4 0.0 3 0 0 0 4 47 2 0.0 3 0 0 0 2 48 4 0.0 3 0 0 0 4 49 3 0.0 3 0 0 0 3 50 7 0.0 3 0 0 0 7 51 38 0.0 3 0 0 1 37 53 2 0.0 3 0 0 0 2 54 2 0.0 3 0 0 1 1 55 1 0.0 3 0 0 0 1 56 4 0.0 3 0 0 0 4 57 1 0.0 3 0 0 0 1 58 7 0.0 3 0 0 0 7 59 5 0.0 3 0 0 0 5 60 4 0.0 3 0 0 0 4 61 3 0.0 3 0 0 1 2 64 5 0.0 3 0 0 0 5 65 4 0.0 3 0 0 0 4 66 2 0.0 3 0 0 0 2 67 3 0.0 3 0 0 0 3 68 10 0.0 3 0 0 0 10 69 3 0.0 3 0 0 0 3 71 1 0.0 3 0 0 0 1 74 2 0.0 3 0 0 0 2 77 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 85 30 0.0 3 0 0 0 30 86 6 0.0 3 0 0 0 6 88 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 90 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 92 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 1 1 102 2 0.0 3 0 0 0 2 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 110 25 0.0 3 0 0 3 22 111 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 113 3 0.0 3 0 0 0 3 114 2 0.0 3 0 0 0 2 115 2 0.0 3 0 0 0 2 116 4 0.0 3 0 0 1 3 117 1 0.0 3 0 0 0 1 118 4 0.0 3 0 0 0 4 121 1 0.0 3 0 0 1 123 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 135 6 0.0 3 0 0 0 6 136 8 0.0 3 0 0 1 7 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 142 3 0.0 3 0 0 0 3 143 2 0.0 3 0 0 0 2 146 2 0.0 3 0 0 0 2 148 4 0.0 3 0 0 0 4 150 1 0.0 3 0 0 0 1 152 3 0.0 3 0 0 0 3 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 161 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 172 6 0.0 3 0 0 0 6 173 5 0.0 3 0 0 1 4 174 27 0.0 3 0 0 0 27 175 6 0.0 3 0 0 0 6 176 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 181 4 0.0 3 0 0 0 4 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 0 2 193 1 0.0 3 0 0 0 1 194 3 0.0 3 0 0 1 2 204 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 249 2 0.0 3 0 0 0 2 270 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.42 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 11,475 Reads with adapters: 199 (1.7%) Reads written (passing filters): 11,475 (100.0%) Total basepairs processed: 3,150,502 bp Total written (filtered): 3,149,899 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 199 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 13.1% C: 72.9% G: 10.6% T: 3.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 196 179.3 0 196 5 3 11.2 0 3