This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.54 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 78,041 (3.2%) Reads that were too short: 53,554 (2.2%) Reads written (passing filters): 24,487 (1.0%) Total basepairs processed: 735,190,694 bp Total written (filtered): 6,183,305 bp (0.8%) === Adapter 1 === Sequence: TGTGGC; Type: regular 5'; Length: 6; Trimmed: 78041 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 343 596.3 0 343 7 7588 596.3 0 7588 8 187 596.3 0 187 9 6732 596.3 0 6732 10 43 596.3 0 43 11 56 596.3 0 56 12 414 596.3 0 414 13 26 596.3 0 26 14 14 596.3 0 14 15 17 596.3 0 17 16 21 596.3 0 21 17 105 596.3 0 105 18 33 596.3 0 33 19 44 596.3 0 44 20 21 596.3 0 21 21 26 596.3 0 26 22 21 596.3 0 21 23 34 596.3 0 34 24 28 596.3 0 28 25 17 596.3 0 17 26 19 596.3 0 19 27 15 596.3 0 15 28 24 596.3 0 24 29 40 596.3 0 40 30 65 596.3 0 65 31 40 596.3 0 40 32 56 596.3 0 56 33 38 596.3 0 38 34 28 596.3 0 28 35 19 596.3 0 19 36 27 596.3 0 27 37 29 596.3 0 29 38 31 596.3 0 31 39 42 596.3 0 42 40 41 596.3 0 41 41 29 596.3 0 29 42 33 596.3 0 33 43 35 596.3 0 35 44 18 596.3 0 18 45 18 596.3 0 18 46 21 596.3 0 21 47 34 596.3 0 34 48 42 596.3 0 42 49 39 596.3 0 39 50 19 596.3 0 19 51 41 596.3 0 41 52 29 596.3 0 29 53 84 596.3 0 84 54 51 596.3 0 51 55 23 596.3 0 23 56 20 596.3 0 20 57 31 596.3 0 31 58 27 596.3 0 27 59 32 596.3 0 32 60 51 596.3 0 51 61 38 596.3 0 38 62 27 596.3 0 27 63 33 596.3 0 33 64 29 596.3 0 29 65 18 596.3 0 18 66 30 596.3 0 30 67 29 596.3 0 29 68 19 596.3 0 19 69 23 596.3 0 23 70 34 596.3 0 34 71 45 596.3 0 45 72 71 596.3 0 71 73 125 596.3 0 125 74 29 596.3 0 29 75 31 596.3 0 31 76 19 596.3 0 19 77 26 596.3 0 26 78 28 596.3 0 28 79 29 596.3 0 29 80 27 596.3 0 27 81 42 596.3 0 42 82 50 596.3 0 50 83 37 596.3 0 37 84 31 596.3 0 31 85 28 596.3 0 28 86 41 596.3 0 41 87 22 596.3 0 22 88 27 596.3 0 27 89 41 596.3 0 41 90 41 596.3 0 41 91 33 596.3 0 33 92 40 596.3 0 40 93 21 596.3 0 21 94 45 596.3 0 45 95 61 596.3 0 61 96 38 596.3 0 38 97 21 596.3 0 21 98 27 596.3 0 27 99 30 596.3 0 30 100 20 596.3 0 20 101 26 596.3 0 26 102 42 596.3 0 42 103 27 596.3 0 27 104 24 596.3 0 24 105 23 596.3 0 23 106 24 596.3 0 24 107 24 596.3 0 24 108 50 596.3 0 50 109 16 596.3 0 16 110 43 596.3 0 43 111 34 596.3 0 34 112 30 596.3 0 30 113 23 596.3 0 23 114 20 596.3 0 20 115 25 596.3 0 25 116 35 596.3 0 35 117 63 596.3 0 63 118 38 596.3 0 38 119 44 596.3 0 44 120 27 596.3 0 27 121 42 596.3 0 42 122 28 596.3 0 28 123 211 596.3 0 211 124 41 596.3 0 41 125 57 596.3 0 57 126 22 596.3 0 22 127 34 596.3 0 34 128 68 596.3 0 68 129 59 596.3 0 59 130 343 596.3 0 343 131 392 596.3 0 392 132 641 596.3 0 641 133 233 596.3 0 233 134 71 596.3 0 71 135 74 596.3 0 74 136 65 596.3 0 65 137 185 596.3 0 185 138 297 596.3 0 297 139 123 596.3 0 123 140 37 596.3 0 37 141 49 596.3 0 49 142 45 596.3 0 45 143 28 596.3 0 28 144 30 596.3 0 30 145 33 596.3 0 33 146 65 596.3 0 65 147 37 596.3 0 37 148 18 596.3 0 18 149 34 596.3 0 34 150 42 596.3 0 42 151 124 596.3 0 124 152 100 596.3 0 100 153 73 596.3 0 73 154 29 596.3 0 29 155 21 596.3 0 21 156 24 596.3 0 24 157 32 596.3 0 32 158 33 596.3 0 33 159 31 596.3 0 31 160 26 596.3 0 26 161 21 596.3 0 21 162 22 596.3 0 22 163 27 596.3 0 27 164 30 596.3 0 30 165 36 596.3 0 36 166 24 596.3 0 24 167 18 596.3 0 18 168 35 596.3 0 35 169 21 596.3 0 21 170 29 596.3 0 29 171 29 596.3 0 29 172 25 596.3 0 25 173 20 596.3 0 20 174 26 596.3 0 26 175 17 596.3 0 17 176 21 596.3 0 21 177 51 596.3 0 51 178 29 596.3 0 29 179 50 596.3 0 50 180 16 596.3 0 16 181 29 596.3 0 29 182 23 596.3 0 23 183 33 596.3 0 33 184 47 596.3 0 47 185 39 596.3 0 39 186 108 596.3 0 108 187 119 596.3 0 119 188 99 596.3 0 99 189 64 596.3 0 64 190 56 596.3 0 56 191 27 596.3 0 27 192 32 596.3 0 32 193 47 596.3 0 47 194 42 596.3 0 42 195 30 596.3 0 30 196 39 596.3 0 39 197 47 596.3 0 47 198 50 596.3 0 50 199 45 596.3 0 45 200 36 596.3 0 36 201 26 596.3 0 26 202 27 596.3 0 27 203 42 596.3 0 42 204 48 596.3 0 48 205 62 596.3 0 62 206 43 596.3 0 43 207 32 596.3 0 32 208 41 596.3 0 41 209 21 596.3 0 21 210 58 596.3 0 58 211 53 596.3 0 53 212 351 596.3 0 351 213 351 596.3 0 351 214 323 596.3 0 323 215 49 596.3 0 49 216 122 596.3 0 122 217 179 596.3 0 179 218 159 596.3 0 159 219 112 596.3 0 112 220 67 596.3 0 67 221 38 596.3 0 38 222 24 596.3 0 24 223 38 596.3 0 38 224 47 596.3 0 47 225 45 596.3 0 45 226 47 596.3 0 47 227 42 596.3 0 42 228 55 596.3 0 55 229 51 596.3 0 51 230 66 596.3 0 66 231 48 596.3 0 48 232 62 596.3 0 62 233 92 596.3 0 92 234 76 596.3 0 76 235 167 596.3 0 167 236 208 596.3 0 208 237 322 596.3 0 322 238 456 596.3 0 456 239 153 596.3 0 153 240 54 596.3 0 54 241 160 596.3 0 160 242 279 596.3 0 279 243 932 596.3 0 932 244 1602 596.3 0 1602 245 1235 596.3 0 1235 246 590 596.3 0 590 247 242 596.3 0 242 248 138 596.3 0 138 249 136 596.3 0 136 250 158 596.3 0 158 251 166 596.3 0 166 252 142 596.3 0 142 253 97 596.3 0 97 254 137 596.3 0 137 255 202 596.3 0 202 256 420 596.3 0 420 257 583 596.3 0 583 258 260 596.3 0 260 259 216 596.3 0 216 260 673 596.3 0 673 261 1623 596.3 0 1623 262 972 596.3 0 972 263 279 596.3 0 279 264 112 596.3 0 112 265 151 596.3 0 151 266 162 596.3 0 162 267 163 596.3 0 163 268 178 596.3 0 178 269 62 596.3 0 62 270 107 596.3 0 107 271 129 596.3 0 129 272 219 596.3 0 219 273 308 596.3 0 308 274 127 596.3 0 127 275 188 596.3 0 188 276 249 596.3 0 249 277 180 596.3 0 180 278 236 596.3 0 236 279 221 596.3 0 221 280 266 596.3 0 266 281 307 596.3 0 307 282 348 596.3 0 348 283 365 596.3 0 365 284 477 596.3 0 477 285 843 596.3 0 843 286 12361 596.3 0 12361 287 9309 596.3 0 9309 288 7802 596.3 0 7802 289 102 596.3 0 102 290 96 596.3 0 96 291 121 596.3 0 121 292 149 596.3 0 149 293 126 596.3 0 126 294 197 596.3 0 197 295 227 596.3 0 227 296 291 596.3 0 291 297 525 596.3 0 525 298 575 596.3 0 575 299 461 596.3 0 461 300 287 596.3 0 287 301 54 596.3 0 54 Finished in 1.18 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 24,487 Reads with adapters: 8,897 (36.3%) Reads that were too short: 69 (0.3%) Reads written (passing filters): 8,828 (36.1%) Total basepairs processed: 6,183,305 bp Total written (filtered): 2,417,386 bp (39.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 8897 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 5 0.0 3 0 0 3 2 16 62 0.0 3 0 57 2 3 17 6826 0.0 3 6290 448 77 11 18 64 0.0 3 5 49 5 5 19 1769 0.0 3 1637 97 17 18 20 26 0.0 3 7 6 2 11 21 3 0.0 3 0 0 1 2 22 3 0.0 3 0 0 0 3 29 1 0.0 3 0 0 0 1 31 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 41 19 0.0 3 0 0 2 17 44 8 0.0 3 0 0 0 8 45 2 0.0 3 0 0 0 2 48 4 0.0 3 0 0 0 4 50 2 0.0 3 0 0 0 2 53 3 0.0 3 0 0 0 3 57 3 0.0 3 0 0 0 3 59 1 0.0 3 0 0 0 1 61 10 0.0 3 0 0 0 10 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 74 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 112 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 1 0.0 3 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 152 2 0.0 3 0 0 1 1 153 4 0.0 3 0 0 0 4 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 159 2 0.0 3 0 0 0 2 161 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 164 8 0.0 3 0 0 1 7 171 15 0.0 3 0 0 2 13 188 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 196 2 0.0 3 0 0 0 2 202 2 0.0 3 0 0 0 2 221 1 0.0 3 0 0 0 1 225 2 0.0 3 0 0 1 1 251 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.31 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 8,828 Reads with adapters: 93 (1.1%) Reads written (passing filters): 8,828 (100.0%) Total basepairs processed: 2,417,386 bp Total written (filtered): 2,417,102 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 93 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 17.2% C: 39.8% G: 36.6% T: 6.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 88 137.9 0 88 4 5 34.5 0 5