This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.53 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 109,394 (4.5%) Reads that were too short: 19,785 (0.8%) Reads written (passing filters): 89,609 (3.7%) Total basepairs processed: 735,190,694 bp Total written (filtered): 24,074,678 bp (3.3%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 109394 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 481 596.3 0 481 7 1530 596.3 0 1530 8 38912 596.3 0 38912 9 30483 596.3 0 30483 10 80 596.3 0 80 11 27 596.3 0 27 12 35 596.3 0 35 13 31 596.3 0 31 14 34 596.3 0 34 15 33 596.3 0 33 16 44 596.3 0 44 17 34 596.3 0 34 18 34 596.3 0 34 19 31 596.3 0 31 20 20 596.3 0 20 21 46 596.3 0 46 22 41 596.3 0 41 23 47 596.3 0 47 24 40 596.3 0 40 25 42 596.3 0 42 26 29 596.3 0 29 27 29 596.3 0 29 28 43 596.3 0 43 29 30 596.3 0 30 30 31 596.3 0 31 31 22 596.3 0 22 32 42 596.3 0 42 33 39 596.3 0 39 34 35 596.3 0 35 35 34 596.3 0 34 36 36 596.3 0 36 37 60 596.3 0 60 38 121 596.3 0 121 39 52 596.3 0 52 40 74 596.3 0 74 41 45 596.3 0 45 42 42 596.3 0 42 43 27 596.3 0 27 44 38 596.3 0 38 45 33 596.3 0 33 46 27 596.3 0 27 47 37 596.3 0 37 48 42 596.3 0 42 49 22 596.3 0 22 50 40 596.3 0 40 51 41 596.3 0 41 52 54 596.3 0 54 53 33 596.3 0 33 54 31 596.3 0 31 55 74 596.3 0 74 56 38 596.3 0 38 57 46 596.3 0 46 58 46 596.3 0 46 59 24 596.3 0 24 60 61 596.3 0 61 61 43 596.3 0 43 62 39 596.3 0 39 63 51 596.3 0 51 64 46 596.3 0 46 65 40 596.3 0 40 66 41 596.3 0 41 67 56 596.3 0 56 68 64 596.3 0 64 69 47 596.3 0 47 70 42 596.3 0 42 71 57 596.3 0 57 72 33 596.3 0 33 73 34 596.3 0 34 74 69 596.3 0 69 75 68 596.3 0 68 76 63 596.3 0 63 77 29 596.3 0 29 78 68 596.3 0 68 79 51 596.3 0 51 80 66 596.3 0 66 81 39 596.3 0 39 82 40 596.3 0 40 83 52 596.3 0 52 84 50 596.3 0 50 85 44 596.3 0 44 86 53 596.3 0 53 87 57 596.3 0 57 88 58 596.3 0 58 89 59 596.3 0 59 90 61 596.3 0 61 91 134 596.3 0 134 92 69 596.3 0 69 93 66 596.3 0 66 94 128 596.3 0 128 95 75 596.3 0 75 96 79 596.3 0 79 97 487 596.3 0 487 98 506 596.3 0 506 99 662 596.3 0 662 100 348 596.3 0 348 101 42 596.3 0 42 102 31 596.3 0 31 103 36 596.3 0 36 104 47 596.3 0 47 105 52 596.3 0 52 106 64 596.3 0 64 107 72 596.3 0 72 108 94 596.3 0 94 109 127 596.3 0 127 110 91 596.3 0 91 111 652 596.3 0 652 112 516 596.3 0 516 113 540 596.3 0 540 114 44 596.3 0 44 115 43 596.3 0 43 116 54 596.3 0 54 117 39 596.3 0 39 118 43 596.3 0 43 119 45 596.3 0 45 120 38 596.3 0 38 121 30 596.3 0 30 122 42 596.3 0 42 123 42 596.3 0 42 124 32 596.3 0 32 125 59 596.3 0 59 126 67 596.3 0 67 127 44 596.3 0 44 128 46 596.3 0 46 129 72 596.3 0 72 130 93 596.3 0 93 131 100 596.3 0 100 132 187 596.3 0 187 133 261 596.3 0 261 134 498 596.3 0 498 135 148 596.3 0 148 136 93 596.3 0 93 137 64 596.3 0 64 138 51 596.3 0 51 139 50 596.3 0 50 140 91 596.3 0 91 141 98 596.3 0 98 142 60 596.3 0 60 143 40 596.3 0 40 144 65 596.3 0 65 145 64 596.3 0 64 146 215 596.3 0 215 147 110 596.3 0 110 148 116 596.3 0 116 149 64 596.3 0 64 150 57 596.3 0 57 151 61 596.3 0 61 152 54 596.3 0 54 153 63 596.3 0 63 154 153 596.3 0 153 155 253 596.3 0 253 156 384 596.3 0 384 157 208 596.3 0 208 158 56 596.3 0 56 159 43 596.3 0 43 160 39 596.3 0 39 161 45 596.3 0 45 162 51 596.3 0 51 163 46 596.3 0 46 164 61 596.3 0 61 165 42 596.3 0 42 166 198 596.3 0 198 167 151 596.3 0 151 168 184 596.3 0 184 169 47 596.3 0 47 170 40 596.3 0 40 171 40 596.3 0 40 172 47 596.3 0 47 173 31 596.3 0 31 174 53 596.3 0 53 175 54 596.3 0 54 176 65 596.3 0 65 177 50 596.3 0 50 178 50 596.3 0 50 179 57 596.3 0 57 180 46 596.3 0 46 181 56 596.3 0 56 182 57 596.3 0 57 183 45 596.3 0 45 184 37 596.3 0 37 185 72 596.3 0 72 186 60 596.3 0 60 187 47 596.3 0 47 188 59 596.3 0 59 189 61 596.3 0 61 190 65 596.3 0 65 191 56 596.3 0 56 192 67 596.3 0 67 193 227 596.3 0 227 194 792 596.3 0 792 195 664 596.3 0 664 196 691 596.3 0 691 197 137 596.3 0 137 198 72 596.3 0 72 199 91 596.3 0 91 200 170 596.3 0 170 201 205 596.3 0 205 202 182 596.3 0 182 203 91 596.3 0 91 204 69 596.3 0 69 205 57 596.3 0 57 206 67 596.3 0 67 207 101 596.3 0 101 208 98 596.3 0 98 209 68 596.3 0 68 210 68 596.3 0 68 211 47 596.3 0 47 212 52 596.3 0 52 213 54 596.3 0 54 214 62 596.3 0 62 215 116 596.3 0 116 216 198 596.3 0 198 217 155 596.3 0 155 218 56 596.3 0 56 219 52 596.3 0 52 220 248 596.3 0 248 221 372 596.3 0 372 222 424 596.3 0 424 223 260 596.3 0 260 224 48 596.3 0 48 225 44 596.3 0 44 226 53 596.3 0 53 227 50 596.3 0 50 228 64 596.3 0 64 229 68 596.3 0 68 230 64 596.3 0 64 231 59 596.3 0 59 232 62 596.3 0 62 233 63 596.3 0 63 234 65 596.3 0 65 235 44 596.3 0 44 236 87 596.3 0 87 237 124 596.3 0 124 238 303 596.3 0 303 239 263 596.3 0 263 240 125 596.3 0 125 241 183 596.3 0 183 242 152 596.3 0 152 243 471 596.3 0 471 244 362 596.3 0 362 245 444 596.3 0 444 246 217 596.3 0 217 247 65 596.3 0 65 248 77 596.3 0 77 249 80 596.3 0 80 250 104 596.3 0 104 251 166 596.3 0 166 252 192 596.3 0 192 253 161 596.3 0 161 254 650 596.3 0 650 255 847 596.3 0 847 256 925 596.3 0 925 257 160 596.3 0 160 258 166 596.3 0 166 259 157 596.3 0 157 260 134 596.3 0 134 261 214 596.3 0 214 262 1385 596.3 0 1385 263 1238 596.3 0 1238 264 1578 596.3 0 1578 265 123 596.3 0 123 266 44 596.3 0 44 267 45 596.3 0 45 268 74 596.3 0 74 269 113 596.3 0 113 270 101 596.3 0 101 271 128 596.3 0 128 272 104 596.3 0 104 273 76 596.3 0 76 274 87 596.3 0 87 275 144 596.3 0 144 276 119 596.3 0 119 277 81 596.3 0 81 278 84 596.3 0 84 279 108 596.3 0 108 280 218 596.3 0 218 281 165 596.3 0 165 282 180 596.3 0 180 283 156 596.3 0 156 284 77 596.3 0 77 285 107 596.3 0 107 286 86 596.3 0 86 287 69 596.3 0 69 288 116 596.3 0 116 289 172 596.3 0 172 290 193 596.3 0 193 291 193 596.3 0 193 292 110 596.3 0 110 293 558 596.3 0 558 294 455 596.3 0 455 295 166 596.3 0 166 296 120 596.3 0 120 297 89 596.3 0 89 298 88 596.3 0 88 299 104 596.3 0 104 300 171 596.3 0 171 301 150 596.3 0 150 Finished in 4.25 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 89,609 Reads with adapters: 40,131 (44.8%) Reads that were too short: 577 (0.6%) Reads written (passing filters): 39,554 (44.1%) Total basepairs processed: 24,074,678 bp Total written (filtered): 10,898,016 bp (45.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 40131 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 6 0.0 2 0 0 0 6 15 27 0.0 3 0 0 17 10 16 503 0.0 3 0 432 53 18 17 38536 0.0 3 36072 2064 334 66 18 119 0.0 3 2 101 8 8 19 142 0.0 3 10 7 77 48 20 89 0.0 3 3 0 0 86 21 1 0.0 3 0 0 0 1 22 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 32 1 0.0 3 1 34 1 0.0 3 0 0 0 1 37 11 0.0 3 0 0 0 11 42 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 1 1 48 3 0.0 3 0 0 0 3 52 4 0.0 3 0 0 0 4 54 1 0.0 3 0 0 0 1 55 4 0.0 3 0 0 0 4 57 1 0.0 3 0 0 0 1 60 3 0.0 3 0 0 1 2 61 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 68 3 0.0 3 0 0 0 3 69 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 74 4 0.0 3 0 0 0 4 75 1 0.0 3 0 0 0 1 76 28 0.0 3 0 0 0 28 77 8 0.0 3 0 0 0 8 79 8 0.0 3 0 0 0 8 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 88 4 0.0 3 0 0 1 3 90 1 0.0 3 0 0 0 1 93 13 0.0 3 0 0 0 13 94 7 0.0 3 0 0 0 7 95 1 0.0 3 0 0 0 1 96 2 0.0 3 0 0 0 2 97 4 0.0 3 0 0 0 4 98 1 0.0 3 0 0 0 1 100 3 0.0 3 0 0 0 3 101 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 119 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 128 3 0.0 3 0 0 0 3 129 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 144 4 0.0 3 0 0 1 3 145 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 156 4 0.0 3 0 0 0 4 157 1 0.0 3 0 1 159 2 0.0 3 0 0 0 2 160 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 178 2 0.0 3 0 0 0 2 179 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 188 33 0.0 3 0 0 0 33 189 6 0.0 3 0 0 0 6 190 1 0.0 3 0 0 0 1 192 4 0.0 3 0 0 0 4 194 2 0.0 3 0 0 0 2 195 3 0.0 3 0 0 0 3 204 1 0.0 3 0 0 0 1 216 9 0.0 3 0 0 2 7 217 440 0.0 3 0 0 50 390 218 3 0.0 3 0 0 0 3 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 6 0.0 3 0 0 0 6 282 9 0.0 3 0 0 0 9 283 1 0.0 3 0 0 0 1 284 2 0.0 3 0 0 0 2 286 2 0.0 3 0 0 0 2 288 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.39 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 39,554 Reads with adapters: 605 (1.5%) Reads written (passing filters): 39,554 (100.0%) Total basepairs processed: 10,898,016 bp Total written (filtered): 10,896,150 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 605 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 55.4% C: 23.0% G: 15.4% T: 6.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 565 618.0 0 565 4 30 154.5 0 30 5 9 38.6 0 9 6 1 9.7 0 1