This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCCTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.37 s (27 us/read; 2.21 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 200,914 (8.2%) Reads that were too short: 14,421 (0.6%) Reads written (passing filters): 186,493 (7.6%) Total basepairs processed: 735,190,694 bp Total written (filtered): 43,582,489 bp (5.9%) === Adapter 1 === Sequence: AGCCTC; Type: regular 5'; Length: 6; Trimmed: 200914 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 580 596.3 0 580 7 17203 596.3 0 17203 8 19143 596.3 0 19143 9 72 596.3 0 72 10 64 596.3 0 64 11 60 596.3 0 60 12 90 596.3 0 90 13 71 596.3 0 71 14 46 596.3 0 46 15 56 596.3 0 56 16 63 596.3 0 63 17 58 596.3 0 58 18 74 596.3 0 74 19 109 596.3 0 109 20 84 596.3 0 84 21 85 596.3 0 85 22 75 596.3 0 75 23 74 596.3 0 74 24 69 596.3 0 69 25 63 596.3 0 63 26 84 596.3 0 84 27 61 596.3 0 61 28 105 596.3 0 105 29 286 596.3 0 286 30 52 596.3 0 52 31 48 596.3 0 48 32 52 596.3 0 52 33 66 596.3 0 66 34 56 596.3 0 56 35 64 596.3 0 64 36 105 596.3 0 105 37 83 596.3 0 83 38 171 596.3 0 171 39 441 596.3 0 441 40 117 596.3 0 117 41 162 596.3 0 162 42 85 596.3 0 85 43 160 596.3 0 160 44 399 596.3 0 399 45 158 596.3 0 158 46 171 596.3 0 171 47 90 596.3 0 90 48 108 596.3 0 108 49 213 596.3 0 213 50 249 596.3 0 249 51 381 596.3 0 381 52 607 596.3 0 607 53 616 596.3 0 616 54 1167 596.3 0 1167 55 2271 596.3 0 2271 56 33619 596.3 0 33619 57 27556 596.3 0 27556 58 42508 596.3 0 42508 59 211 596.3 0 211 60 61 596.3 0 61 61 64 596.3 0 64 62 116 596.3 0 116 63 86 596.3 0 86 64 139 596.3 0 139 65 39 596.3 0 39 66 50 596.3 0 50 67 50 596.3 0 50 68 37 596.3 0 37 69 71 596.3 0 71 70 53 596.3 0 53 71 50 596.3 0 50 72 58 596.3 0 58 73 63 596.3 0 63 74 67 596.3 0 67 75 61 596.3 0 61 76 63 596.3 0 63 77 35 596.3 0 35 78 45 596.3 0 45 79 48 596.3 0 48 80 50 596.3 0 50 81 122 596.3 0 122 82 51 596.3 0 51 83 67 596.3 0 67 84 65 596.3 0 65 85 76 596.3 0 76 86 55 596.3 0 55 87 55 596.3 0 55 88 84 596.3 0 84 89 106 596.3 0 106 90 30 596.3 0 30 91 29 596.3 0 29 92 41 596.3 0 41 93 49 596.3 0 49 94 41 596.3 0 41 95 40 596.3 0 40 96 45 596.3 0 45 97 47 596.3 0 47 98 37 596.3 0 37 99 53 596.3 0 53 100 54 596.3 0 54 101 53 596.3 0 53 102 39 596.3 0 39 103 113 596.3 0 113 104 159 596.3 0 159 105 122 596.3 0 122 106 45 596.3 0 45 107 36 596.3 0 36 108 47 596.3 0 47 109 58 596.3 0 58 110 53 596.3 0 53 111 64 596.3 0 64 112 46 596.3 0 46 113 61 596.3 0 61 114 54 596.3 0 54 115 36 596.3 0 36 116 42 596.3 0 42 117 46 596.3 0 46 118 61 596.3 0 61 119 66 596.3 0 66 120 59 596.3 0 59 121 53 596.3 0 53 122 45 596.3 0 45 123 64 596.3 0 64 124 330 596.3 0 330 125 277 596.3 0 277 126 798 596.3 0 798 127 81 596.3 0 81 128 113 596.3 0 113 129 53 596.3 0 53 130 73 596.3 0 73 131 66 596.3 0 66 132 57 596.3 0 57 133 56 596.3 0 56 134 53 596.3 0 53 135 66 596.3 0 66 136 48 596.3 0 48 137 44 596.3 0 44 138 81 596.3 0 81 139 65 596.3 0 65 140 88 596.3 0 88 141 84 596.3 0 84 142 81 596.3 0 81 143 236 596.3 0 236 144 76 596.3 0 76 145 106 596.3 0 106 146 204 596.3 0 204 147 1237 596.3 0 1237 148 1223 596.3 0 1223 149 1259 596.3 0 1259 150 903 596.3 0 903 151 351 596.3 0 351 152 340 596.3 0 340 153 75 596.3 0 75 154 125 596.3 0 125 155 80 596.3 0 80 156 95 596.3 0 95 157 56 596.3 0 56 158 50 596.3 0 50 159 76 596.3 0 76 160 540 596.3 0 540 161 108 596.3 0 108 162 333 596.3 0 333 163 101 596.3 0 101 164 85 596.3 0 85 165 461 596.3 0 461 166 202 596.3 0 202 167 2169 596.3 0 2169 168 1548 596.3 0 1548 169 2289 596.3 0 2289 170 121 596.3 0 121 171 97 596.3 0 97 172 143 596.3 0 143 173 179 596.3 0 179 174 110 596.3 0 110 175 174 596.3 0 174 176 212 596.3 0 212 177 249 596.3 0 249 178 138 596.3 0 138 179 60 596.3 0 60 180 93 596.3 0 93 181 84 596.3 0 84 182 89 596.3 0 89 183 175 596.3 0 175 184 68 596.3 0 68 185 102 596.3 0 102 186 87 596.3 0 87 187 401 596.3 0 401 188 167 596.3 0 167 189 145 596.3 0 145 190 109 596.3 0 109 191 104 596.3 0 104 192 166 596.3 0 166 193 128 596.3 0 128 194 128 596.3 0 128 195 282 596.3 0 282 196 383 596.3 0 383 197 327 596.3 0 327 198 231 596.3 0 231 199 572 596.3 0 572 200 6027 596.3 0 6027 201 3764 596.3 0 3764 202 5777 596.3 0 5777 203 262 596.3 0 262 204 51 596.3 0 51 205 48 596.3 0 48 206 49 596.3 0 49 207 62 596.3 0 62 208 51 596.3 0 51 209 34 596.3 0 34 210 40 596.3 0 40 211 54 596.3 0 54 212 49 596.3 0 49 213 54 596.3 0 54 214 51 596.3 0 51 215 64 596.3 0 64 216 47 596.3 0 47 217 45 596.3 0 45 218 44 596.3 0 44 219 80 596.3 0 80 220 89 596.3 0 89 221 96 596.3 0 96 222 41 596.3 0 41 223 45 596.3 0 45 224 36 596.3 0 36 225 38 596.3 0 38 226 51 596.3 0 51 227 48 596.3 0 48 228 77 596.3 0 77 229 219 596.3 0 219 230 172 596.3 0 172 231 270 596.3 0 270 232 294 596.3 0 294 233 174 596.3 0 174 234 253 596.3 0 253 235 51 596.3 0 51 236 40 596.3 0 40 237 36 596.3 0 36 238 39 596.3 0 39 239 71 596.3 0 71 240 52 596.3 0 52 241 89 596.3 0 89 242 66 596.3 0 66 243 55 596.3 0 55 244 47 596.3 0 47 245 46 596.3 0 46 246 41 596.3 0 41 247 57 596.3 0 57 248 82 596.3 0 82 249 76 596.3 0 76 250 64 596.3 0 64 251 46 596.3 0 46 252 56 596.3 0 56 253 40 596.3 0 40 254 48 596.3 0 48 255 57 596.3 0 57 256 40 596.3 0 40 257 66 596.3 0 66 258 61 596.3 0 61 259 54 596.3 0 54 260 48 596.3 0 48 261 144 596.3 0 144 262 55 596.3 0 55 263 49 596.3 0 49 264 70 596.3 0 70 265 66 596.3 0 66 266 60 596.3 0 60 267 56 596.3 0 56 268 120 596.3 0 120 269 126 596.3 0 126 270 137 596.3 0 137 271 86 596.3 0 86 272 51 596.3 0 51 273 97 596.3 0 97 274 83 596.3 0 83 275 127 596.3 0 127 276 92 596.3 0 92 277 103 596.3 0 103 278 172 596.3 0 172 279 493 596.3 0 493 280 134 596.3 0 134 281 162 596.3 0 162 282 181 596.3 0 181 283 178 596.3 0 178 284 76 596.3 0 76 285 40 596.3 0 40 286 52 596.3 0 52 287 62 596.3 0 62 288 92 596.3 0 92 289 93 596.3 0 93 290 62 596.3 0 62 291 78 596.3 0 78 292 63 596.3 0 63 293 111 596.3 0 111 294 91 596.3 0 91 295 100 596.3 0 100 296 53 596.3 0 53 297 60 596.3 0 60 298 46 596.3 0 46 299 49 596.3 0 49 300 105 596.3 0 105 301 153 596.3 0 153 Finished in 8.49 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 186,493 Reads with adapters: 19,869 (10.7%) Reads that were too short: 15 (0.0%) Reads written (passing filters): 19,854 (10.6%) Total basepairs processed: 43,582,489 bp Total written (filtered): 5,391,106 bp (12.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 19869 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 3 19 211 0.0 3 0 200 11 20 19152 0.0 4 18795 335 20 1 1 21 44 0.0 4 8 36 22 6 0.0 4 0 2 4 23 153 0.0 4 141 0 1 10 1 24 1 0.0 4 0 1 25 1 0.0 4 0 0 0 0 1 26 12 0.0 4 0 0 0 0 12 29 1 0.0 4 0 0 0 0 1 54 1 0.0 4 1 69 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 1 92 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 122 9 0.0 4 0 0 0 0 9 126 4 0.0 4 0 0 0 0 4 127 249 0.0 4 0 0 0 0 249 128 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 134 1 0.0 4 1 135 1 0.0 4 0 0 0 0 1 147 1 0.0 4 1 154 1 0.0 4 1 174 1 0.0 4 0 0 0 0 1 177 1 0.0 4 1 202 2 0.0 4 0 0 0 0 2 229 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 1 264 1 0.0 4 0 0 0 0 1 274 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.69 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 19,854 Reads with adapters: 1,476 (7.4%) Reads written (passing filters): 19,854 (100.0%) Total basepairs processed: 5,391,106 bp Total written (filtered): 5,347,287 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1476 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.6% C: 14.6% G: 82.4% T: 1.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 17 310.2 0 17 4 2 77.6 0 2 9 182 0.1 0 4 178 10 281 0.0 1 8 273 11 720 0.0 1 7 713 12 9 0.0 1 1 8 90 173 0.0 1 154 19 91 5 0.0 1 5 92 3 0.0 1 3 157 4 0.0 1 4 177 61 0.0 1 55 6 178 2 0.0 1 2 180 14 0.0 1 13 1 181 1 0.0 1 1 230 1 0.0 1 1 271 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.