This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.69 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 331,400 (13.6%) Reads that were too short: 13,743 (0.6%) Reads written (passing filters): 317,657 (13.0%) Total basepairs processed: 735,190,694 bp Total written (filtered): 85,654,269 bp (11.7%) === Adapter 1 === Sequence: GTCAAT; Type: regular 5'; Length: 6; Trimmed: 331400 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2534 596.3 0 2534 7 81300 596.3 0 81300 8 1497 596.3 0 1497 9 99482 596.3 0 99482 10 92 596.3 0 92 11 41 596.3 0 41 12 43 596.3 0 43 13 40 596.3 0 40 14 32 596.3 0 32 15 39 596.3 0 39 16 41 596.3 0 41 17 49 596.3 0 49 18 70 596.3 0 70 19 55 596.3 0 55 20 46 596.3 0 46 21 54 596.3 0 54 22 51 596.3 0 51 23 64 596.3 0 64 24 66 596.3 0 66 25 58 596.3 0 58 26 63 596.3 0 63 27 77 596.3 0 77 28 159 596.3 0 159 29 227 596.3 0 227 30 106 596.3 0 106 31 142 596.3 0 142 32 344 596.3 0 344 33 114 596.3 0 114 34 118 596.3 0 118 35 179 596.3 0 179 36 167 596.3 0 167 37 198 596.3 0 198 38 356 596.3 0 356 39 285 596.3 0 285 40 313 596.3 0 313 41 453 596.3 0 453 42 7652 596.3 0 7652 43 12156 596.3 0 12156 44 7773 596.3 0 7773 45 88 596.3 0 88 46 56 596.3 0 56 47 126 596.3 0 126 48 293 596.3 0 293 49 90 596.3 0 90 50 164 596.3 0 164 51 97 596.3 0 97 52 114 596.3 0 114 53 316 596.3 0 316 54 141 596.3 0 141 55 140 596.3 0 140 56 84 596.3 0 84 57 122 596.3 0 122 58 179 596.3 0 179 59 222 596.3 0 222 60 381 596.3 0 381 61 616 596.3 0 616 62 698 596.3 0 698 63 1261 596.3 0 1261 64 2405 596.3 0 2405 65 29475 596.3 0 29475 66 18444 596.3 0 18444 67 37983 596.3 0 37983 68 173 596.3 0 173 69 61 596.3 0 61 70 50 596.3 0 50 71 71 596.3 0 71 72 45 596.3 0 45 73 45 596.3 0 45 74 36 596.3 0 36 75 33 596.3 0 33 76 60 596.3 0 60 77 69 596.3 0 69 78 44 596.3 0 44 79 38 596.3 0 38 80 46 596.3 0 46 81 51 596.3 0 51 82 30 596.3 0 30 83 34 596.3 0 34 84 35 596.3 0 35 85 47 596.3 0 47 86 35 596.3 0 35 87 132 596.3 0 132 88 39 596.3 0 39 89 56 596.3 0 56 90 27 596.3 0 27 91 52 596.3 0 52 92 29 596.3 0 29 93 56 596.3 0 56 94 52 596.3 0 52 95 49 596.3 0 49 96 56 596.3 0 56 97 50 596.3 0 50 98 68 596.3 0 68 99 51 596.3 0 51 100 54 596.3 0 54 101 70 596.3 0 70 102 118 596.3 0 118 103 37 596.3 0 37 104 48 596.3 0 48 105 34 596.3 0 34 106 43 596.3 0 43 107 48 596.3 0 48 108 44 596.3 0 44 109 40 596.3 0 40 110 52 596.3 0 52 111 59 596.3 0 59 112 48 596.3 0 48 113 25 596.3 0 25 114 40 596.3 0 40 115 43 596.3 0 43 116 28 596.3 0 28 117 32 596.3 0 32 118 42 596.3 0 42 119 49 596.3 0 49 120 33 596.3 0 33 121 44 596.3 0 44 122 44 596.3 0 44 123 41 596.3 0 41 124 48 596.3 0 48 125 28 596.3 0 28 126 39 596.3 0 39 127 49 596.3 0 49 128 44 596.3 0 44 129 46 596.3 0 46 130 50 596.3 0 50 131 34 596.3 0 34 132 58 596.3 0 58 133 60 596.3 0 60 134 204 596.3 0 204 135 257 596.3 0 257 136 171 596.3 0 171 137 97 596.3 0 97 138 477 596.3 0 477 139 242 596.3 0 242 140 66 596.3 0 66 141 41 596.3 0 41 142 32 596.3 0 32 143 46 596.3 0 46 144 44 596.3 0 44 145 38 596.3 0 38 146 38 596.3 0 38 147 45 596.3 0 45 148 60 596.3 0 60 149 46 596.3 0 46 150 28 596.3 0 28 151 29 596.3 0 29 152 34 596.3 0 34 153 41 596.3 0 41 154 38 596.3 0 38 155 41 596.3 0 41 156 45 596.3 0 45 157 52 596.3 0 52 158 40 596.3 0 40 159 55 596.3 0 55 160 54 596.3 0 54 161 46 596.3 0 46 162 26 596.3 0 26 163 34 596.3 0 34 164 32 596.3 0 32 165 31 596.3 0 31 166 33 596.3 0 33 167 46 596.3 0 46 168 48 596.3 0 48 169 40 596.3 0 40 170 37 596.3 0 37 171 30 596.3 0 30 172 38 596.3 0 38 173 37 596.3 0 37 174 45 596.3 0 45 175 39 596.3 0 39 176 35 596.3 0 35 177 35 596.3 0 35 178 55 596.3 0 55 179 73 596.3 0 73 180 74 596.3 0 74 181 70 596.3 0 70 182 58 596.3 0 58 183 60 596.3 0 60 184 87 596.3 0 87 185 71 596.3 0 71 186 93 596.3 0 93 187 32 596.3 0 32 188 33 596.3 0 33 189 39 596.3 0 39 190 40 596.3 0 40 191 52 596.3 0 52 192 37 596.3 0 37 193 30 596.3 0 30 194 30 596.3 0 30 195 28 596.3 0 28 196 46 596.3 0 46 197 69 596.3 0 69 198 86 596.3 0 86 199 58 596.3 0 58 200 53 596.3 0 53 201 67 596.3 0 67 202 106 596.3 0 106 203 100 596.3 0 100 204 57 596.3 0 57 205 42 596.3 0 42 206 35 596.3 0 35 207 52 596.3 0 52 208 47 596.3 0 47 209 62 596.3 0 62 210 52 596.3 0 52 211 49 596.3 0 49 212 57 596.3 0 57 213 38 596.3 0 38 214 37 596.3 0 37 215 77 596.3 0 77 216 82 596.3 0 82 217 69 596.3 0 69 218 84 596.3 0 84 219 79 596.3 0 79 220 74 596.3 0 74 221 77 596.3 0 77 222 363 596.3 0 363 223 495 596.3 0 495 224 444 596.3 0 444 225 270 596.3 0 270 226 38 596.3 0 38 227 48 596.3 0 48 228 66 596.3 0 66 229 49 596.3 0 49 230 41 596.3 0 41 231 43 596.3 0 43 232 42 596.3 0 42 233 45 596.3 0 45 234 48 596.3 0 48 235 43 596.3 0 43 236 52 596.3 0 52 237 45 596.3 0 45 238 68 596.3 0 68 239 47 596.3 0 47 240 73 596.3 0 73 241 73 596.3 0 73 242 151 596.3 0 151 243 297 596.3 0 297 244 213 596.3 0 213 245 131 596.3 0 131 246 44 596.3 0 44 247 45 596.3 0 45 248 51 596.3 0 51 249 63 596.3 0 63 250 119 596.3 0 119 251 98 596.3 0 98 252 111 596.3 0 111 253 181 596.3 0 181 254 204 596.3 0 204 255 1127 596.3 0 1127 256 934 596.3 0 934 257 1246 596.3 0 1246 258 276 596.3 0 276 259 165 596.3 0 165 260 400 596.3 0 400 261 191 596.3 0 191 262 321 596.3 0 321 263 117 596.3 0 117 264 122 596.3 0 122 265 185 596.3 0 185 266 183 596.3 0 183 267 85 596.3 0 85 268 71 596.3 0 71 269 70 596.3 0 70 270 387 596.3 0 387 271 200 596.3 0 200 272 227 596.3 0 227 273 60 596.3 0 60 274 90 596.3 0 90 275 52 596.3 0 52 276 87 596.3 0 87 277 102 596.3 0 102 278 129 596.3 0 129 279 150 596.3 0 150 280 74 596.3 0 74 281 44 596.3 0 44 282 45 596.3 0 45 283 46 596.3 0 46 284 49 596.3 0 49 285 34 596.3 0 34 286 43 596.3 0 43 287 58 596.3 0 58 288 46 596.3 0 46 289 33 596.3 0 33 290 45 596.3 0 45 291 61 596.3 0 61 292 77 596.3 0 77 293 74 596.3 0 74 294 81 596.3 0 81 295 37 596.3 0 37 296 40 596.3 0 40 297 67 596.3 0 67 298 52 596.3 0 52 299 92 596.3 0 92 300 233 596.3 0 233 301 58 596.3 0 58 Finished in 13.91 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 317,657 Reads with adapters: 103,023 (32.4%) Reads that were too short: 296 (0.1%) Reads written (passing filters): 102,727 (32.3%) Total basepairs processed: 85,654,269 bp Total written (filtered): 28,236,896 bp (33.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 103023 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 7 0.0 2 0 0 0 7 15 66 0.0 3 0 0 44 22 16 1060 0.0 3 0 941 78 41 17 100090 0.0 3 93824 5307 786 173 18 215 0.0 3 13 174 14 14 19 197 0.0 3 0 10 97 90 20 961 0.0 3 737 49 8 167 21 5 0.0 3 0 4 0 1 22 1 0.0 3 0 0 0 1 23 6 0.0 3 3 0 0 3 24 1 0.0 3 0 0 0 1 31 1 0.0 3 1 34 4 0.0 3 1 0 0 3 35 1 0.0 3 1 36 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 1 53 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 57 3 0.0 3 0 0 0 3 58 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 61 8 0.0 3 0 0 0 8 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 80 3 0.0 3 1 0 0 2 81 3 0.0 3 0 0 0 3 82 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 88 3 0.0 3 1 1 0 1 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 3 0.0 3 0 0 0 3 103 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 0 2 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 124 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 1 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 143 11 0.0 3 0 0 0 11 144 9 0.0 3 0 0 0 9 145 1 0.0 3 0 0 0 1 146 4 0.0 3 0 0 0 4 147 8 0.0 3 0 0 0 8 148 2 0.0 3 0 0 0 2 149 2 0.0 3 0 0 0 2 150 5 0.0 3 0 0 0 5 152 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 2 0.0 3 0 0 0 2 160 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 165 1 0.0 3 0 0 0 1 166 3 0.0 3 0 0 0 3 167 10 0.0 3 0 0 0 10 168 7 0.0 3 0 0 0 7 170 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 176 2 0.0 3 0 0 0 2 178 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 4 0.0 3 0 0 0 4 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 0 2 192 2 0.0 3 0 0 0 2 193 1 0.0 3 0 0 0 1 194 5 0.0 3 0 0 0 5 196 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 204 3 0.0 3 0 0 0 3 205 4 0.0 3 0 0 0 4 206 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 213 3 0.0 3 0 0 0 3 216 2 0.0 3 0 0 0 2 217 129 0.0 3 0 0 14 115 218 2 0.0 3 0 1 0 1 220 1 0.0 3 0 0 0 1 221 4 0.0 3 0 0 0 4 222 2 0.0 3 0 0 0 2 223 4 0.0 3 0 0 0 4 224 3 0.0 3 0 0 0 3 226 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 230 3 0.0 3 0 0 0 3 231 2 0.0 3 0 0 0 2 233 2 0.0 3 0 0 0 2 237 2 0.0 3 0 0 0 2 238 2 0.0 3 0 0 0 2 246 1 0.0 3 0 0 0 1 251 1 0.0 3 0 0 1 252 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 262 7 0.0 3 0 0 1 6 266 2 0.0 3 0 0 0 2 268 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 9 0.0 3 0 0 0 9 281 10 0.0 3 0 0 0 10 282 15 0.0 3 0 0 0 15 283 3 0.0 3 0 0 1 2 284 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 288 2 0.0 3 0 0 0 2 291 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.69 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 102,727 Reads with adapters: 7,256 (7.1%) Reads written (passing filters): 102,727 (100.0%) Total basepairs processed: 28,236,896 bp Total written (filtered): 28,214,929 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 7256 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 66.2% C: 16.9% G: 14.4% T: 2.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 7080 1605.1 0 7080 4 168 401.3 0 168 5 3 100.3 0 3 6 3 25.1 0 3 10 1 0.1 1 0 1 12 1 0.0 1 0 1