This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.38 s (27 us/read; 2.21 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 355,465 (14.6%) Reads that were too short: 9,716 (0.4%) Reads written (passing filters): 345,749 (14.2%) Total basepairs processed: 735,190,694 bp Total written (filtered): 92,726,355 bp (12.6%) === Adapter 1 === Sequence: GTCAAT; Type: regular 5'; Length: 6; Trimmed: 355465 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2515 596.3 0 2515 7 106031 596.3 0 106031 8 919 596.3 0 919 9 82761 596.3 0 82761 10 108 596.3 0 108 11 64 596.3 0 64 12 60 596.3 0 60 13 82 596.3 0 82 14 48 596.3 0 48 15 63 596.3 0 63 16 89 596.3 0 89 17 86 596.3 0 86 18 81 596.3 0 81 19 71 596.3 0 71 20 77 596.3 0 77 21 84 596.3 0 84 22 61 596.3 0 61 23 84 596.3 0 84 24 81 596.3 0 81 25 100 596.3 0 100 26 99 596.3 0 99 27 97 596.3 0 97 28 191 596.3 0 191 29 229 596.3 0 229 30 140 596.3 0 140 31 142 596.3 0 142 32 395 596.3 0 395 33 126 596.3 0 126 34 143 596.3 0 143 35 213 596.3 0 213 36 179 596.3 0 179 37 199 596.3 0 199 38 388 596.3 0 388 39 238 596.3 0 238 40 307 596.3 0 307 41 373 596.3 0 373 42 7654 596.3 0 7654 43 12490 596.3 0 12490 44 7579 596.3 0 7579 45 116 596.3 0 116 46 93 596.3 0 93 47 154 596.3 0 154 48 418 596.3 0 418 49 146 596.3 0 146 50 208 596.3 0 208 51 108 596.3 0 108 52 142 596.3 0 142 53 390 596.3 0 390 54 156 596.3 0 156 55 231 596.3 0 231 56 112 596.3 0 112 57 169 596.3 0 169 58 206 596.3 0 206 59 236 596.3 0 236 60 362 596.3 0 362 61 646 596.3 0 646 62 779 596.3 0 779 63 1340 596.3 0 1340 64 2620 596.3 0 2620 65 35602 596.3 0 35602 66 22355 596.3 0 22355 67 45808 596.3 0 45808 68 241 596.3 0 241 69 82 596.3 0 82 70 59 596.3 0 59 71 86 596.3 0 86 72 64 596.3 0 64 73 72 596.3 0 72 74 52 596.3 0 52 75 62 596.3 0 62 76 70 596.3 0 70 77 68 596.3 0 68 78 58 596.3 0 58 79 64 596.3 0 64 80 59 596.3 0 59 81 62 596.3 0 62 82 51 596.3 0 51 83 58 596.3 0 58 84 60 596.3 0 60 85 60 596.3 0 60 86 57 596.3 0 57 87 123 596.3 0 123 88 72 596.3 0 72 89 72 596.3 0 72 90 66 596.3 0 66 91 68 596.3 0 68 92 48 596.3 0 48 93 64 596.3 0 64 94 74 596.3 0 74 95 91 596.3 0 91 96 52 596.3 0 52 97 49 596.3 0 49 98 73 596.3 0 73 99 83 596.3 0 83 100 78 596.3 0 78 101 62 596.3 0 62 102 77 596.3 0 77 103 59 596.3 0 59 104 67 596.3 0 67 105 44 596.3 0 44 106 58 596.3 0 58 107 79 596.3 0 79 108 78 596.3 0 78 109 66 596.3 0 66 110 70 596.3 0 70 111 70 596.3 0 70 112 66 596.3 0 66 113 58 596.3 0 58 114 75 596.3 0 75 115 59 596.3 0 59 116 57 596.3 0 57 117 49 596.3 0 49 118 38 596.3 0 38 119 55 596.3 0 55 120 63 596.3 0 63 121 64 596.3 0 64 122 52 596.3 0 52 123 54 596.3 0 54 124 65 596.3 0 65 125 66 596.3 0 66 126 57 596.3 0 57 127 56 596.3 0 56 128 54 596.3 0 54 129 61 596.3 0 61 130 53 596.3 0 53 131 55 596.3 0 55 132 59 596.3 0 59 133 67 596.3 0 67 134 109 596.3 0 109 135 107 596.3 0 107 136 100 596.3 0 100 137 75 596.3 0 75 138 478 596.3 0 478 139 253 596.3 0 253 140 75 596.3 0 75 141 40 596.3 0 40 142 52 596.3 0 52 143 51 596.3 0 51 144 53 596.3 0 53 145 42 596.3 0 42 146 50 596.3 0 50 147 65 596.3 0 65 148 54 596.3 0 54 149 48 596.3 0 48 150 40 596.3 0 40 151 35 596.3 0 35 152 27 596.3 0 27 153 42 596.3 0 42 154 46 596.3 0 46 155 33 596.3 0 33 156 55 596.3 0 55 157 51 596.3 0 51 158 44 596.3 0 44 159 70 596.3 0 70 160 57 596.3 0 57 161 53 596.3 0 53 162 49 596.3 0 49 163 47 596.3 0 47 164 55 596.3 0 55 165 52 596.3 0 52 166 52 596.3 0 52 167 45 596.3 0 45 168 58 596.3 0 58 169 61 596.3 0 61 170 63 596.3 0 63 171 50 596.3 0 50 172 47 596.3 0 47 173 54 596.3 0 54 174 60 596.3 0 60 175 41 596.3 0 41 176 43 596.3 0 43 177 46 596.3 0 46 178 63 596.3 0 63 179 74 596.3 0 74 180 65 596.3 0 65 181 54 596.3 0 54 182 43 596.3 0 43 183 52 596.3 0 52 184 50 596.3 0 50 185 60 596.3 0 60 186 59 596.3 0 59 187 49 596.3 0 49 188 53 596.3 0 53 189 56 596.3 0 56 190 48 596.3 0 48 191 54 596.3 0 54 192 41 596.3 0 41 193 38 596.3 0 38 194 37 596.3 0 37 195 38 596.3 0 38 196 51 596.3 0 51 197 59 596.3 0 59 198 69 596.3 0 69 199 47 596.3 0 47 200 59 596.3 0 59 201 47 596.3 0 47 202 61 596.3 0 61 203 55 596.3 0 55 204 48 596.3 0 48 205 58 596.3 0 58 206 59 596.3 0 59 207 54 596.3 0 54 208 53 596.3 0 53 209 45 596.3 0 45 210 52 596.3 0 52 211 49 596.3 0 49 212 43 596.3 0 43 213 34 596.3 0 34 214 41 596.3 0 41 215 53 596.3 0 53 216 74 596.3 0 74 217 59 596.3 0 59 218 56 596.3 0 56 219 66 596.3 0 66 220 61 596.3 0 61 221 57 596.3 0 57 222 126 596.3 0 126 223 177 596.3 0 177 224 158 596.3 0 158 225 141 596.3 0 141 226 52 596.3 0 52 227 42 596.3 0 42 228 60 596.3 0 60 229 41 596.3 0 41 230 49 596.3 0 49 231 42 596.3 0 42 232 40 596.3 0 40 233 47 596.3 0 47 234 51 596.3 0 51 235 51 596.3 0 51 236 69 596.3 0 69 237 61 596.3 0 61 238 64 596.3 0 64 239 57 596.3 0 57 240 40 596.3 0 40 241 41 596.3 0 41 242 113 596.3 0 113 243 160 596.3 0 160 244 103 596.3 0 103 245 154 596.3 0 154 246 68 596.3 0 68 247 70 596.3 0 70 248 56 596.3 0 56 249 52 596.3 0 52 250 133 596.3 0 133 251 69 596.3 0 69 252 86 596.3 0 86 253 106 596.3 0 106 254 148 596.3 0 148 255 632 596.3 0 632 256 545 596.3 0 545 257 683 596.3 0 683 258 142 596.3 0 142 259 76 596.3 0 76 260 336 596.3 0 336 261 188 596.3 0 188 262 310 596.3 0 310 263 112 596.3 0 112 264 81 596.3 0 81 265 108 596.3 0 108 266 164 596.3 0 164 267 53 596.3 0 53 268 71 596.3 0 71 269 58 596.3 0 58 270 384 596.3 0 384 271 119 596.3 0 119 272 135 596.3 0 135 273 64 596.3 0 64 274 63 596.3 0 63 275 76 596.3 0 76 276 64 596.3 0 64 277 61 596.3 0 61 278 68 596.3 0 68 279 83 596.3 0 83 280 53 596.3 0 53 281 55 596.3 0 55 282 37 596.3 0 37 283 33 596.3 0 33 284 49 596.3 0 49 285 35 596.3 0 35 286 40 596.3 0 40 287 44 596.3 0 44 288 46 596.3 0 46 289 36 596.3 0 36 290 52 596.3 0 52 291 50 596.3 0 50 292 68 596.3 0 68 293 51 596.3 0 51 294 39 596.3 0 39 295 36 596.3 0 36 296 49 596.3 0 49 297 39 596.3 0 39 298 61 596.3 0 61 299 87 596.3 0 87 300 200 596.3 0 200 301 75 596.3 0 75 Finished in 16.26 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 345,749 Reads with adapters: 110,560 (32.0%) Reads that were too short: 92 (0.0%) Reads written (passing filters): 110,468 (32.0%) Total basepairs processed: 92,726,355 bp Total written (filtered): 29,867,263 bp (32.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 110560 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 18 32 0.0 3 0 0 31 1 19 971 0.0 3 0 939 28 3 1 20 105304 0.0 4 103502 1751 48 3 21 176 0.0 4 26 150 22 26 0.0 4 3 4 15 0 4 23 288 0.0 4 218 5 0 56 9 24 2 0.0 4 2 26 1 0.0 4 0 0 0 1 29 1 0.0 4 0 0 0 1 30 1 0.0 4 1 46 1 0.0 4 0 0 0 0 1 64 1 0.0 4 1 65 1 0.0 4 1 66 1 0.0 4 1 81 1 0.0 4 1 91 1 0.0 4 0 0 0 0 1 92 8 0.0 4 0 0 0 0 8 100 1 0.0 4 0 0 0 0 1 106 1 0.0 4 1 107 25 0.0 4 0 0 0 0 25 108 1 0.0 4 0 0 0 0 1 113 27 0.0 4 0 0 0 0 27 115 3 0.0 4 0 0 0 0 3 125 4 0.0 4 0 0 0 0 4 126 46 0.0 4 0 0 0 0 46 127 3514 0.0 4 0 0 0 0 3514 128 8 0.0 4 0 0 0 0 8 130 17 0.0 4 0 0 0 0 17 131 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 1 144 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 1 167 3 0.0 4 0 0 0 0 3 168 1 0.0 4 0 0 0 1 177 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 193 42 0.0 4 0 0 0 1 41 194 18 0.0 4 0 0 0 1 17 195 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 1 216 1 0.0 4 0 0 0 0 1 228 8 0.0 4 0 0 0 0 8 229 3 0.0 4 0 0 0 0 3 239 2 0.0 4 0 0 0 0 2 257 1 0.0 4 0 0 0 0 1 270 1 0.0 4 0 0 0 0 1 282 2 0.0 4 0 0 0 0 2 284 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.77 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 110,468 Reads with adapters: 3,500 (3.2%) Reads written (passing filters): 110,468 (100.0%) Total basepairs processed: 29,867,263 bp Total written (filtered): 29,790,605 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3500 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 6.8% C: 8.9% G: 82.6% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 278 1726.1 0 278 4 21 431.5 0 21 5 6 107.9 0 6 9 24 0.4 0 3 21 10 39 0.1 1 1 38 11 2706 0.0 1 19 2687 12 256 0.0 1 2 254 13 3 0.0 1 2 1 15 3 0.0 1 0 3 30 1 0.0 1 0 1 55 1 0.0 1 0 1 72 1 0.0 1 1 83 1 0.0 1 1 84 1 0.0 1 1 225 3 0.0 1 2 1 259 1 0.0 1 1 263 1 0.0 1 1 264 149 0.0 1 142 7 265 5 0.0 1 5 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.