This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTATGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.26 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 339,145 (13.9%) Reads that were too short: 18,686 (0.8%) Reads written (passing filters): 320,459 (13.1%) Total basepairs processed: 735,190,694 bp Total written (filtered): 91,066,051 bp (12.4%) === Adapter 1 === Sequence: GTATGT; Type: regular 5'; Length: 6; Trimmed: 339145 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2096 596.3 0 2096 7 2864 596.3 0 2864 8 161366 596.3 0 161366 9 131669 596.3 0 131669 10 168 596.3 0 168 11 52 596.3 0 52 12 53 596.3 0 53 13 44 596.3 0 44 14 40 596.3 0 40 15 24 596.3 0 24 16 34 596.3 0 34 17 49 596.3 0 49 18 39 596.3 0 39 19 56 596.3 0 56 20 50 596.3 0 50 21 40 596.3 0 40 22 48 596.3 0 48 23 35 596.3 0 35 24 191 596.3 0 191 25 53 596.3 0 53 26 42 596.3 0 42 27 33 596.3 0 33 28 47 596.3 0 47 29 39 596.3 0 39 30 51 596.3 0 51 31 49 596.3 0 49 32 25 596.3 0 25 33 33 596.3 0 33 34 35 596.3 0 35 35 32 596.3 0 32 36 101 596.3 0 101 37 32 596.3 0 32 38 29 596.3 0 29 39 48 596.3 0 48 40 40 596.3 0 40 41 32 596.3 0 32 42 24 596.3 0 24 43 43 596.3 0 43 44 40 596.3 0 40 45 37 596.3 0 37 46 32 596.3 0 32 47 30 596.3 0 30 48 33 596.3 0 33 49 31 596.3 0 31 50 40 596.3 0 40 51 30 596.3 0 30 52 38 596.3 0 38 53 41 596.3 0 41 54 47 596.3 0 47 55 41 596.3 0 41 56 34 596.3 0 34 57 38 596.3 0 38 58 50 596.3 0 50 59 34 596.3 0 34 60 31 596.3 0 31 61 21 596.3 0 21 62 25 596.3 0 25 63 46 596.3 0 46 64 36 596.3 0 36 65 67 596.3 0 67 66 51 596.3 0 51 67 69 596.3 0 69 68 33 596.3 0 33 69 100 596.3 0 100 70 71 596.3 0 71 71 157 596.3 0 157 72 44 596.3 0 44 73 40 596.3 0 40 74 34 596.3 0 34 75 54 596.3 0 54 76 71 596.3 0 71 77 98 596.3 0 98 78 149 596.3 0 149 79 102 596.3 0 102 80 477 596.3 0 477 81 136 596.3 0 136 82 176 596.3 0 176 83 78 596.3 0 78 84 54 596.3 0 54 85 58 596.3 0 58 86 92 596.3 0 92 87 56 596.3 0 56 88 35 596.3 0 35 89 73 596.3 0 73 90 68 596.3 0 68 91 64 596.3 0 64 92 63 596.3 0 63 93 81 596.3 0 81 94 74 596.3 0 74 95 71 596.3 0 71 96 1068 596.3 0 1068 97 896 596.3 0 896 98 1204 596.3 0 1204 99 93 596.3 0 93 100 84 596.3 0 84 101 50 596.3 0 50 102 45 596.3 0 45 103 42 596.3 0 42 104 41 596.3 0 41 105 44 596.3 0 44 106 46 596.3 0 46 107 38 596.3 0 38 108 37 596.3 0 37 109 55 596.3 0 55 110 71 596.3 0 71 111 48 596.3 0 48 112 66 596.3 0 66 113 59 596.3 0 59 114 78 596.3 0 78 115 48 596.3 0 48 116 37 596.3 0 37 117 44 596.3 0 44 118 52 596.3 0 52 119 45 596.3 0 45 120 52 596.3 0 52 121 37 596.3 0 37 122 55 596.3 0 55 123 40 596.3 0 40 124 48 596.3 0 48 125 56 596.3 0 56 126 71 596.3 0 71 127 86 596.3 0 86 128 135 596.3 0 135 129 139 596.3 0 139 130 431 596.3 0 431 131 204 596.3 0 204 132 291 596.3 0 291 133 240 596.3 0 240 134 221 596.3 0 221 135 67 596.3 0 67 136 56 596.3 0 56 137 91 596.3 0 91 138 101 596.3 0 101 139 201 596.3 0 201 140 80 596.3 0 80 141 72 596.3 0 72 142 74 596.3 0 74 143 60 596.3 0 60 144 71 596.3 0 71 145 66 596.3 0 66 146 72 596.3 0 72 147 92 596.3 0 92 148 1225 596.3 0 1225 149 329 596.3 0 329 150 245 596.3 0 245 151 54 596.3 0 54 152 52 596.3 0 52 153 43 596.3 0 43 154 73 596.3 0 73 155 58 596.3 0 58 156 49 596.3 0 49 157 70 596.3 0 70 158 70 596.3 0 70 159 70 596.3 0 70 160 53 596.3 0 53 161 55 596.3 0 55 162 38 596.3 0 38 163 43 596.3 0 43 164 46 596.3 0 46 165 49 596.3 0 49 166 44 596.3 0 44 167 54 596.3 0 54 168 38 596.3 0 38 169 49 596.3 0 49 170 41 596.3 0 41 171 40 596.3 0 40 172 48 596.3 0 48 173 57 596.3 0 57 174 58 596.3 0 58 175 44 596.3 0 44 176 47 596.3 0 47 177 57 596.3 0 57 178 75 596.3 0 75 179 49 596.3 0 49 180 53 596.3 0 53 181 70 596.3 0 70 182 54 596.3 0 54 183 81 596.3 0 81 184 105 596.3 0 105 185 167 596.3 0 167 186 213 596.3 0 213 187 1324 596.3 0 1324 188 2486 596.3 0 2486 189 888 596.3 0 888 190 380 596.3 0 380 191 49 596.3 0 49 192 72 596.3 0 72 193 41 596.3 0 41 194 67 596.3 0 67 195 62 596.3 0 62 196 48 596.3 0 48 197 43 596.3 0 43 198 49 596.3 0 49 199 36 596.3 0 36 200 52 596.3 0 52 201 39 596.3 0 39 202 53 596.3 0 53 203 59 596.3 0 59 204 63 596.3 0 63 205 79 596.3 0 79 206 74 596.3 0 74 207 91 596.3 0 91 208 75 596.3 0 75 209 426 596.3 0 426 210 561 596.3 0 561 211 606 596.3 0 606 212 141 596.3 0 141 213 69 596.3 0 69 214 59 596.3 0 59 215 53 596.3 0 53 216 56 596.3 0 56 217 55 596.3 0 55 218 62 596.3 0 62 219 68 596.3 0 68 220 65 596.3 0 65 221 57 596.3 0 57 222 62 596.3 0 62 223 100 596.3 0 100 224 90 596.3 0 90 225 95 596.3 0 95 226 209 596.3 0 209 227 134 596.3 0 134 228 101 596.3 0 101 229 83 596.3 0 83 230 114 596.3 0 114 231 89 596.3 0 89 232 107 596.3 0 107 233 145 596.3 0 145 234 152 596.3 0 152 235 266 596.3 0 266 236 186 596.3 0 186 237 125 596.3 0 125 238 114 596.3 0 114 239 131 596.3 0 131 240 419 596.3 0 419 241 494 596.3 0 494 242 937 596.3 0 937 243 508 596.3 0 508 244 220 596.3 0 220 245 114 596.3 0 114 246 302 596.3 0 302 247 179 596.3 0 179 248 163 596.3 0 163 249 335 596.3 0 335 250 315 596.3 0 315 251 389 596.3 0 389 252 244 596.3 0 244 253 515 596.3 0 515 254 1082 596.3 0 1082 255 881 596.3 0 881 256 252 596.3 0 252 257 112 596.3 0 112 258 174 596.3 0 174 259 146 596.3 0 146 260 315 596.3 0 315 261 293 596.3 0 293 262 550 596.3 0 550 263 117 596.3 0 117 264 69 596.3 0 69 265 137 596.3 0 137 266 122 596.3 0 122 267 142 596.3 0 142 268 165 596.3 0 165 269 148 596.3 0 148 270 241 596.3 0 241 271 310 596.3 0 310 272 362 596.3 0 362 273 310 596.3 0 310 274 441 596.3 0 441 275 198 596.3 0 198 276 47 596.3 0 47 277 34 596.3 0 34 278 56 596.3 0 56 279 75 596.3 0 75 280 58 596.3 0 58 281 83 596.3 0 83 282 88 596.3 0 88 283 192 596.3 0 192 284 119 596.3 0 119 285 71 596.3 0 71 286 47 596.3 0 47 287 44 596.3 0 44 288 42 596.3 0 42 289 44 596.3 0 44 290 49 596.3 0 49 291 44 596.3 0 44 292 72 596.3 0 72 293 88 596.3 0 88 294 95 596.3 0 95 295 69 596.3 0 69 296 73 596.3 0 73 297 75 596.3 0 75 298 86 596.3 0 86 299 55 596.3 0 55 300 63 596.3 0 63 301 141 596.3 0 141 Finished in 14.02 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 320,459 Reads with adapters: 161,551 (50.4%) Reads that were too short: 303 (0.1%) Reads written (passing filters): 161,248 (50.3%) Total basepairs processed: 91,066,051 bp Total written (filtered): 44,481,364 bp (48.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 161551 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 17 0.0 2 0 0 0 17 15 136 0.0 3 0 0 87 49 16 1906 0.0 3 0 1650 156 100 17 157612 0.0 3 147821 8157 1353 281 18 426 0.0 3 14 347 40 25 19 555 0.0 3 2 1 312 240 20 410 0.0 3 142 11 2 255 21 2 0.0 3 1 1 22 5 0.0 3 1 1 1 2 25 2 0.0 3 2 26 1 0.0 3 0 0 0 1 32 1 0.0 3 0 1 34 12 0.0 3 0 0 0 12 37 3 0.0 3 0 0 0 3 38 1 0.0 3 0 0 0 1 42 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 0 1 44 3 0.0 3 0 0 0 3 45 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 49 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 58 3 0.0 3 0 0 0 3 59 10 0.0 3 0 0 0 10 60 1 0.0 3 0 0 0 1 61 8 0.0 3 0 0 1 7 62 2 0.0 3 0 0 0 2 63 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 0 2 70 49 0.0 3 0 0 0 49 71 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 73 8 0.0 3 0 0 0 8 75 1 0.0 3 0 0 1 76 2 0.0 3 0 0 0 2 77 2 0.0 3 0 0 0 2 78 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 87 4 0.0 3 0 0 0 4 89 1 0.0 3 0 0 0 1 90 3 0.0 3 0 0 1 2 91 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 96 4 0.0 3 0 0 0 4 98 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 108 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 114 2 0.0 3 0 0 0 2 121 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 128 3 0.0 3 0 0 1 2 130 4 0.0 3 0 0 0 4 144 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 160 1 0.0 3 0 0 0 1 161 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 163 7 0.0 3 0 0 0 7 164 9 0.0 3 0 0 0 9 165 2 0.0 3 0 0 0 2 167 4 0.0 3 0 0 0 4 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 182 3 0.0 3 0 0 0 3 183 8 0.0 3 0 0 0 8 185 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 187 2 0.0 3 0 0 0 2 190 1 0.0 3 0 0 0 1 192 6 0.0 3 0 0 0 6 193 2 0.0 3 0 0 0 2 194 11 0.0 3 0 0 1 10 195 3 0.0 3 0 0 1 2 198 1 0.0 3 0 0 0 1 205 7 0.0 3 0 0 0 7 208 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 216 3 0.0 3 0 0 0 3 217 119 0.0 3 0 0 10 109 221 3 0.0 3 0 0 0 3 222 2 0.0 3 0 0 0 2 236 1 0.0 3 0 0 0 1 237 2 0.0 3 0 0 0 2 238 2 0.0 3 0 0 0 2 239 2 0.0 3 0 0 0 2 246 1 0.0 3 0 0 0 1 252 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 271 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 13 0.0 3 0 0 0 13 280 22 0.0 3 0 0 0 22 281 21 0.0 3 0 0 0 21 282 14 0.0 3 0 0 1 13 283 4 0.0 3 0 0 0 4 284 1 0.0 3 0 0 0 1 285 2 0.0 3 0 0 0 2 286 1 0.0 3 0 0 0 1 288 4 0.0 3 0 0 0 4 289 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.64 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 161,248 Reads with adapters: 1,401 (0.9%) Reads written (passing filters): 161,248 (100.0%) Total basepairs processed: 44,481,364 bp Total written (filtered): 44,476,900 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1401 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 52.0% C: 17.4% G: 23.0% T: 7.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1256 2519.5 0 1256 4 61 629.9 0 61 5 75 157.5 0 75 6 5 39.4 0 5 11 1 0.0 1 0 1 12 3 0.0 1 0 3