This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTTGCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.27 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 67,507 (2.8%) Reads that were too short: 12,209 (0.5%) Reads written (passing filters): 55,298 (2.3%) Total basepairs processed: 735,190,694 bp Total written (filtered): 14,454,675 bp (2.0%) === Adapter 1 === Sequence: GTTGCC; Type: regular 5'; Length: 6; Trimmed: 67507 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 539 596.3 0 539 7 21251 596.3 0 21251 8 17094 596.3 0 17094 9 42 596.3 0 42 10 69 596.3 0 69 11 24 596.3 0 24 12 24 596.3 0 24 13 18 596.3 0 18 14 27 596.3 0 27 15 18 596.3 0 18 16 15 596.3 0 15 17 17 596.3 0 17 18 13 596.3 0 13 19 25 596.3 0 25 20 33 596.3 0 33 21 33 596.3 0 33 22 25 596.3 0 25 23 20 596.3 0 20 24 25 596.3 0 25 25 17 596.3 0 17 26 11 596.3 0 11 27 12 596.3 0 12 28 11 596.3 0 11 29 18 596.3 0 18 30 27 596.3 0 27 31 17 596.3 0 17 32 23 596.3 0 23 33 14 596.3 0 14 34 16 596.3 0 16 35 22 596.3 0 22 36 36 596.3 0 36 37 35 596.3 0 35 38 71 596.3 0 71 39 37 596.3 0 37 40 66 596.3 0 66 41 21 596.3 0 21 42 25 596.3 0 25 43 35 596.3 0 35 44 19 596.3 0 19 45 18 596.3 0 18 46 15 596.3 0 15 47 15 596.3 0 15 48 157 596.3 0 157 49 48 596.3 0 48 50 21 596.3 0 21 51 28 596.3 0 28 52 13 596.3 0 13 53 33 596.3 0 33 54 22 596.3 0 22 55 35 596.3 0 35 56 24 596.3 0 24 57 21 596.3 0 21 58 16 596.3 0 16 59 25 596.3 0 25 60 52 596.3 0 52 61 59 596.3 0 59 62 36 596.3 0 36 63 19 596.3 0 19 64 40 596.3 0 40 65 33 596.3 0 33 66 26 596.3 0 26 67 27 596.3 0 27 68 45 596.3 0 45 69 175 596.3 0 175 70 252 596.3 0 252 71 171 596.3 0 171 72 426 596.3 0 426 73 615 596.3 0 615 74 308 596.3 0 308 75 31 596.3 0 31 76 19 596.3 0 19 77 18 596.3 0 18 78 27 596.3 0 27 79 28 596.3 0 28 80 48 596.3 0 48 81 87 596.3 0 87 82 146 596.3 0 146 83 128 596.3 0 128 84 368 596.3 0 368 85 1098 596.3 0 1098 86 2418 596.3 0 2418 87 30 596.3 0 30 88 22 596.3 0 22 89 21 596.3 0 21 90 75 596.3 0 75 91 61 596.3 0 61 92 26 596.3 0 26 93 20 596.3 0 20 94 24 596.3 0 24 95 17 596.3 0 17 96 37 596.3 0 37 97 51 596.3 0 51 98 21 596.3 0 21 99 31 596.3 0 31 100 33 596.3 0 33 101 32 596.3 0 32 102 39 596.3 0 39 103 27 596.3 0 27 104 25 596.3 0 25 105 30 596.3 0 30 106 24 596.3 0 24 107 22 596.3 0 22 108 32 596.3 0 32 109 20 596.3 0 20 110 71 596.3 0 71 111 304 596.3 0 304 112 334 596.3 0 334 113 30 596.3 0 30 114 25 596.3 0 25 115 28 596.3 0 28 116 24 596.3 0 24 117 19 596.3 0 19 118 20 596.3 0 20 119 18 596.3 0 18 120 16 596.3 0 16 121 30 596.3 0 30 122 27 596.3 0 27 123 37 596.3 0 37 124 52 596.3 0 52 125 31 596.3 0 31 126 18 596.3 0 18 127 21 596.3 0 21 128 22 596.3 0 22 129 28 596.3 0 28 130 34 596.3 0 34 131 53 596.3 0 53 132 58 596.3 0 58 133 47 596.3 0 47 134 28 596.3 0 28 135 24 596.3 0 24 136 32 596.3 0 32 137 30 596.3 0 30 138 37 596.3 0 37 139 31 596.3 0 31 140 22 596.3 0 22 141 27 596.3 0 27 142 50 596.3 0 50 143 209 596.3 0 209 144 60 596.3 0 60 145 86 596.3 0 86 146 75 596.3 0 75 147 131 596.3 0 131 148 152 596.3 0 152 149 104 596.3 0 104 150 214 596.3 0 214 151 83 596.3 0 83 152 82 596.3 0 82 153 72 596.3 0 72 154 21 596.3 0 21 155 34 596.3 0 34 156 80 596.3 0 80 157 103 596.3 0 103 158 119 596.3 0 119 159 38 596.3 0 38 160 29 596.3 0 29 161 32 596.3 0 32 162 28 596.3 0 28 163 31 596.3 0 31 164 27 596.3 0 27 165 34 596.3 0 34 166 37 596.3 0 37 167 35 596.3 0 35 168 129 596.3 0 129 169 100 596.3 0 100 170 133 596.3 0 133 171 48 596.3 0 48 172 23 596.3 0 23 173 24 596.3 0 24 174 37 596.3 0 37 175 36 596.3 0 36 176 199 596.3 0 199 177 151 596.3 0 151 178 237 596.3 0 237 179 23 596.3 0 23 180 17 596.3 0 17 181 22 596.3 0 22 182 27 596.3 0 27 183 49 596.3 0 49 184 101 596.3 0 101 185 30 596.3 0 30 186 36 596.3 0 36 187 92 596.3 0 92 188 118 596.3 0 118 189 114 596.3 0 114 190 64 596.3 0 64 191 51 596.3 0 51 192 32 596.3 0 32 193 32 596.3 0 32 194 58 596.3 0 58 195 82 596.3 0 82 196 160 596.3 0 160 197 314 596.3 0 314 198 708 596.3 0 708 199 600 596.3 0 600 200 98 596.3 0 98 201 53 596.3 0 53 202 233 596.3 0 233 203 409 596.3 0 409 204 442 596.3 0 442 205 396 596.3 0 396 206 193 596.3 0 193 207 46 596.3 0 46 208 42 596.3 0 42 209 78 596.3 0 78 210 83 596.3 0 83 211 52 596.3 0 52 212 66 596.3 0 66 213 158 596.3 0 158 214 154 596.3 0 154 215 162 596.3 0 162 216 92 596.3 0 92 217 66 596.3 0 66 218 74 596.3 0 74 219 83 596.3 0 83 220 51 596.3 0 51 221 51 596.3 0 51 222 124 596.3 0 124 223 773 596.3 0 773 224 655 596.3 0 655 225 476 596.3 0 476 226 40 596.3 0 40 227 33 596.3 0 33 228 44 596.3 0 44 229 34 596.3 0 34 230 22 596.3 0 22 231 22 596.3 0 22 232 21 596.3 0 21 233 33 596.3 0 33 234 65 596.3 0 65 235 67 596.3 0 67 236 74 596.3 0 74 237 55 596.3 0 55 238 113 596.3 0 113 239 88 596.3 0 88 240 50 596.3 0 50 241 79 596.3 0 79 242 169 596.3 0 169 243 106 596.3 0 106 244 113 596.3 0 113 245 160 596.3 0 160 246 145 596.3 0 145 247 52 596.3 0 52 248 50 596.3 0 50 249 37 596.3 0 37 250 61 596.3 0 61 251 48 596.3 0 48 252 43 596.3 0 43 253 47 596.3 0 47 254 37 596.3 0 37 255 52 596.3 0 52 256 47 596.3 0 47 257 82 596.3 0 82 258 146 596.3 0 146 259 207 596.3 0 207 260 192 596.3 0 192 261 61 596.3 0 61 262 52 596.3 0 52 263 49 596.3 0 49 264 43 596.3 0 43 265 39 596.3 0 39 266 33 596.3 0 33 267 31 596.3 0 31 268 28 596.3 0 28 269 34 596.3 0 34 270 70 596.3 0 70 271 256 596.3 0 256 272 193 596.3 0 193 273 166 596.3 0 166 274 48 596.3 0 48 275 38 596.3 0 38 276 54 596.3 0 54 277 104 596.3 0 104 278 112 596.3 0 112 279 76 596.3 0 76 280 44 596.3 0 44 281 77 596.3 0 77 282 214 596.3 0 214 283 293 596.3 0 293 284 439 596.3 0 439 285 114 596.3 0 114 286 117 596.3 0 117 287 78 596.3 0 78 288 45 596.3 0 45 289 48 596.3 0 48 290 40 596.3 0 40 291 36 596.3 0 36 292 84 596.3 0 84 293 65 596.3 0 65 294 72 596.3 0 72 295 54 596.3 0 54 296 90 596.3 0 90 297 207 596.3 0 207 298 347 596.3 0 347 299 434 596.3 0 434 300 153 596.3 0 153 301 78 596.3 0 78 Finished in 2.65 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 55,298 Reads with adapters: 21,133 (38.2%) Reads that were too short: 183 (0.3%) Reads written (passing filters): 20,950 (37.9%) Total basepairs processed: 14,454,675 bp Total written (filtered): 5,792,370 bp (40.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 21133 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 15 0.0 3 0 0 8 7 16 211 0.0 3 0 173 24 14 17 20472 0.0 3 19022 1190 210 50 18 54 0.0 3 7 32 9 6 19 90 0.0 3 1 1 45 43 20 38 0.0 3 1 2 0 35 21 1 0.0 3 0 0 0 1 22 2 0.0 3 1 0 0 1 23 1 0.0 3 0 0 0 1 24 2 0.0 3 0 0 0 2 25 1 0.0 3 0 0 0 1 28 2 0.0 3 0 0 0 2 29 16 0.0 3 0 0 1 15 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 35 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 0 1 40 4 0.0 3 0 0 0 4 41 12 0.0 3 0 0 0 12 43 2 0.0 3 0 0 0 2 44 4 0.0 3 0 0 0 4 47 1 0.0 3 0 0 0 1 48 1 0.0 3 1 49 2 0.0 3 0 0 0 2 52 1 0.0 3 0 1 56 1 0.0 3 0 0 1 60 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 79 3 0.0 3 0 0 1 2 80 2 0.0 3 0 0 0 2 81 8 0.0 3 0 0 1 7 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 89 3 0.0 3 0 0 0 3 93 1 0.0 3 0 0 1 94 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 103 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 2 0.0 3 0 0 0 2 113 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 120 10 0.0 3 0 0 1 9 128 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 140 22 0.0 3 0 0 1 21 142 2 0.0 3 0 0 0 2 148 2 0.0 3 0 0 0 2 149 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 156 6 0.0 3 0 0 0 6 162 3 0.0 3 0 0 0 3 163 2 0.0 3 0 0 0 2 178 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 204 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 216 3 0.0 3 0 0 0 3 217 57 0.0 3 0 0 5 52 218 2 0.0 3 0 0 0 2 221 2 0.0 3 0 0 0 2 226 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 2 0.0 3 0 0 0 2 288 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.74 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 20,950 Reads with adapters: 295 (1.4%) Reads written (passing filters): 20,950 (100.0%) Total basepairs processed: 5,792,370 bp Total written (filtered): 5,791,479 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 295 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 23.4% C: 69.5% G: 5.4% T: 1.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 291 327.3 0 291 4 3 81.8 0 3 6 1 5.1 0 1