This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTTGCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.12 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 56,523 (2.3%) Reads that were too short: 5,935 (0.2%) Reads written (passing filters): 50,588 (2.1%) Total basepairs processed: 735,190,694 bp Total written (filtered): 13,778,183 bp (1.9%) === Adapter 1 === Sequence: GTTGCC; Type: regular 5'; Length: 6; Trimmed: 56523 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 613 596.3 0 613 7 17878 596.3 0 17878 8 21767 596.3 0 21767 9 39 596.3 0 39 10 26 596.3 0 26 11 23 596.3 0 23 12 25 596.3 0 25 13 19 596.3 0 19 14 25 596.3 0 25 15 19 596.3 0 19 16 23 596.3 0 23 17 16 596.3 0 16 18 12 596.3 0 12 19 17 596.3 0 17 20 15 596.3 0 15 21 17 596.3 0 17 22 27 596.3 0 27 23 28 596.3 0 28 24 21 596.3 0 21 25 24 596.3 0 24 26 17 596.3 0 17 27 17 596.3 0 17 28 17 596.3 0 17 29 26 596.3 0 26 30 24 596.3 0 24 31 15 596.3 0 15 32 22 596.3 0 22 33 10 596.3 0 10 34 21 596.3 0 21 35 20 596.3 0 20 36 29 596.3 0 29 37 17 596.3 0 17 38 24 596.3 0 24 39 21 596.3 0 21 40 27 596.3 0 27 41 30 596.3 0 30 42 24 596.3 0 24 43 31 596.3 0 31 44 14 596.3 0 14 45 21 596.3 0 21 46 22 596.3 0 22 47 20 596.3 0 20 48 26 596.3 0 26 49 35 596.3 0 35 50 17 596.3 0 17 51 28 596.3 0 28 52 28 596.3 0 28 53 16 596.3 0 16 54 20 596.3 0 20 55 36 596.3 0 36 56 35 596.3 0 35 57 36 596.3 0 36 58 22 596.3 0 22 59 28 596.3 0 28 60 41 596.3 0 41 61 33 596.3 0 33 62 21 596.3 0 21 63 26 596.3 0 26 64 19 596.3 0 19 65 20 596.3 0 20 66 31 596.3 0 31 67 31 596.3 0 31 68 39 596.3 0 39 69 159 596.3 0 159 70 301 596.3 0 301 71 219 596.3 0 219 72 561 596.3 0 561 73 780 596.3 0 780 74 385 596.3 0 385 75 26 596.3 0 26 76 22 596.3 0 22 77 20 596.3 0 20 78 19 596.3 0 19 79 35 596.3 0 35 80 44 596.3 0 44 81 73 596.3 0 73 82 117 596.3 0 117 83 63 596.3 0 63 84 278 596.3 0 278 85 123 596.3 0 123 86 364 596.3 0 364 87 22 596.3 0 22 88 19 596.3 0 19 89 20 596.3 0 20 90 21 596.3 0 21 91 30 596.3 0 30 92 17 596.3 0 17 93 10 596.3 0 10 94 34 596.3 0 34 95 10 596.3 0 10 96 14 596.3 0 14 97 33 596.3 0 33 98 24 596.3 0 24 99 25 596.3 0 25 100 33 596.3 0 33 101 23 596.3 0 23 102 16 596.3 0 16 103 24 596.3 0 24 104 12 596.3 0 12 105 19 596.3 0 19 106 30 596.3 0 30 107 20 596.3 0 20 108 21 596.3 0 21 109 19 596.3 0 19 110 33 596.3 0 33 111 227 596.3 0 227 112 308 596.3 0 308 113 21 596.3 0 21 114 17 596.3 0 17 115 35 596.3 0 35 116 16 596.3 0 16 117 26 596.3 0 26 118 13 596.3 0 13 119 27 596.3 0 27 120 17 596.3 0 17 121 20 596.3 0 20 122 14 596.3 0 14 123 23 596.3 0 23 124 39 596.3 0 39 125 16 596.3 0 16 126 32 596.3 0 32 127 16 596.3 0 16 128 24 596.3 0 24 129 29 596.3 0 29 130 18 596.3 0 18 131 29 596.3 0 29 132 36 596.3 0 36 133 25 596.3 0 25 134 21 596.3 0 21 135 19 596.3 0 19 136 21 596.3 0 21 137 15 596.3 0 15 138 29 596.3 0 29 139 20 596.3 0 20 140 27 596.3 0 27 141 14 596.3 0 14 142 26 596.3 0 26 143 205 596.3 0 205 144 31 596.3 0 31 145 44 596.3 0 44 146 39 596.3 0 39 147 41 596.3 0 41 148 157 596.3 0 157 149 109 596.3 0 109 150 247 596.3 0 247 151 29 596.3 0 29 152 30 596.3 0 30 153 15 596.3 0 15 154 20 596.3 0 20 155 26 596.3 0 26 156 31 596.3 0 31 157 64 596.3 0 64 158 55 596.3 0 55 159 21 596.3 0 21 160 19 596.3 0 19 161 32 596.3 0 32 162 24 596.3 0 24 163 17 596.3 0 17 164 22 596.3 0 22 165 30 596.3 0 30 166 19 596.3 0 19 167 27 596.3 0 27 168 143 596.3 0 143 169 74 596.3 0 74 170 103 596.3 0 103 171 26 596.3 0 26 172 32 596.3 0 32 173 18 596.3 0 18 174 22 596.3 0 22 175 26 596.3 0 26 176 79 596.3 0 79 177 78 596.3 0 78 178 102 596.3 0 102 179 20 596.3 0 20 180 22 596.3 0 22 181 25 596.3 0 25 182 26 596.3 0 26 183 44 596.3 0 44 184 119 596.3 0 119 185 18 596.3 0 18 186 35 596.3 0 35 187 46 596.3 0 46 188 54 596.3 0 54 189 60 596.3 0 60 190 39 596.3 0 39 191 33 596.3 0 33 192 27 596.3 0 27 193 21 596.3 0 21 194 37 596.3 0 37 195 44 596.3 0 44 196 66 596.3 0 66 197 131 596.3 0 131 198 235 596.3 0 235 199 291 596.3 0 291 200 85 596.3 0 85 201 24 596.3 0 24 202 81 596.3 0 81 203 160 596.3 0 160 204 209 596.3 0 209 205 132 596.3 0 132 206 78 596.3 0 78 207 21 596.3 0 21 208 33 596.3 0 33 209 30 596.3 0 30 210 30 596.3 0 30 211 37 596.3 0 37 212 25 596.3 0 25 213 55 596.3 0 55 214 57 596.3 0 57 215 61 596.3 0 61 216 48 596.3 0 48 217 38 596.3 0 38 218 44 596.3 0 44 219 39 596.3 0 39 220 28 596.3 0 28 221 32 596.3 0 32 222 59 596.3 0 59 223 320 596.3 0 320 224 318 596.3 0 318 225 242 596.3 0 242 226 22 596.3 0 22 227 18 596.3 0 18 228 31 596.3 0 31 229 22 596.3 0 22 230 22 596.3 0 22 231 21 596.3 0 21 232 17 596.3 0 17 233 19 596.3 0 19 234 35 596.3 0 35 235 31 596.3 0 31 236 45 596.3 0 45 237 33 596.3 0 33 238 35 596.3 0 35 239 43 596.3 0 43 240 35 596.3 0 35 241 29 596.3 0 29 242 49 596.3 0 49 243 34 596.3 0 34 244 33 596.3 0 33 245 62 596.3 0 62 246 44 596.3 0 44 247 29 596.3 0 29 248 26 596.3 0 26 249 38 596.3 0 38 250 36 596.3 0 36 251 34 596.3 0 34 252 25 596.3 0 25 253 41 596.3 0 41 254 35 596.3 0 35 255 26 596.3 0 26 256 30 596.3 0 30 257 32 596.3 0 32 258 68 596.3 0 68 259 160 596.3 0 160 260 174 596.3 0 174 261 43 596.3 0 43 262 28 596.3 0 28 263 26 596.3 0 26 264 25 596.3 0 25 265 24 596.3 0 24 266 19 596.3 0 19 267 13 596.3 0 13 268 32 596.3 0 32 269 30 596.3 0 30 270 34 596.3 0 34 271 91 596.3 0 91 272 82 596.3 0 82 273 58 596.3 0 58 274 22 596.3 0 22 275 24 596.3 0 24 276 33 596.3 0 33 277 47 596.3 0 47 278 43 596.3 0 43 279 39 596.3 0 39 280 29 596.3 0 29 281 39 596.3 0 39 282 90 596.3 0 90 283 167 596.3 0 167 284 236 596.3 0 236 285 57 596.3 0 57 286 42 596.3 0 42 287 48 596.3 0 48 288 25 596.3 0 25 289 24 596.3 0 24 290 18 596.3 0 18 291 36 596.3 0 36 292 35 596.3 0 35 293 42 596.3 0 42 294 34 596.3 0 34 295 38 596.3 0 38 296 55 596.3 0 55 297 115 596.3 0 115 298 163 596.3 0 163 299 197 596.3 0 197 300 78 596.3 0 78 301 43 596.3 0 43 Finished in 2.68 s (53 us/read; 1.13 M reads/minute). === Summary === Total reads processed: 50,588 Reads with adapters: 23,886 (47.2%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 23,874 (47.2%) Total basepairs processed: 13,778,183 bp Total written (filtered): 6,379,423 bp (46.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 23886 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 13 0.0 3 0 0 13 19 328 0.0 3 0 319 8 0 1 20 22134 0.0 4 21729 382 21 1 1 21 54 0.0 4 6 45 3 22 2 0.0 4 1 0 1 23 24 0.0 4 0 0 0 21 3 24 1 0.0 4 0 0 0 1 34 1 0.0 4 1 45 1 0.0 4 1 74 2 0.0 4 2 82 1 0.0 4 0 0 0 0 1 104 1 0.0 4 1 105 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 14 0.0 4 0 0 0 0 14 127 1291 0.0 4 0 0 0 0 1291 128 4 0.0 4 0 0 0 0 4 130 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 250 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.84 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 23,874 Reads with adapters: 2,324 (9.7%) Reads written (passing filters): 23,874 (100.0%) Total basepairs processed: 6,379,423 bp Total written (filtered): 6,354,203 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2324 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.8% C: 3.0% G: 94.1% T: 1.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 18 373.0 0 18 4 6 93.3 0 6 5 1 23.3 0 1 9 13 0.1 0 1 12 10 295 0.0 1 6 289 11 1982 0.0 1 13 1969 12 7 0.0 1 0 7 14 1 0.0 1 0 1 170 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.