This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TACTTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.58 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 294,634 (12.1%) Reads that were too short: 50,116 (2.1%) Reads written (passing filters): 244,518 (10.0%) Total basepairs processed: 735,190,694 bp Total written (filtered): 60,895,710 bp (8.3%) === Adapter 1 === Sequence: TACTTC; Type: regular 5'; Length: 6; Trimmed: 294634 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 8732 596.3 0 8732 7 70328 596.3 0 70328 8 1211 596.3 0 1211 9 85421 596.3 0 85421 10 78 596.3 0 78 11 26 596.3 0 26 12 22 596.3 0 22 13 14 596.3 0 14 14 18 596.3 0 18 15 29 596.3 0 29 16 31 596.3 0 31 17 39 596.3 0 39 18 23 596.3 0 23 19 25 596.3 0 25 20 21 596.3 0 21 21 23 596.3 0 23 22 19 596.3 0 19 23 24 596.3 0 24 24 23 596.3 0 23 25 16 596.3 0 16 26 30 596.3 0 30 27 23 596.3 0 23 28 29 596.3 0 29 29 23 596.3 0 23 30 34 596.3 0 34 31 60 596.3 0 60 32 22 596.3 0 22 33 23 596.3 0 23 34 25 596.3 0 25 35 16 596.3 0 16 36 60 596.3 0 60 37 32 596.3 0 32 38 30 596.3 0 30 39 52 596.3 0 52 40 23 596.3 0 23 41 17 596.3 0 17 42 27 596.3 0 27 43 26 596.3 0 26 44 20 596.3 0 20 45 23 596.3 0 23 46 20 596.3 0 20 47 40 596.3 0 40 48 35 596.3 0 35 49 21 596.3 0 21 50 24 596.3 0 24 51 19 596.3 0 19 52 18 596.3 0 18 53 34 596.3 0 34 54 30 596.3 0 30 55 32 596.3 0 32 56 64 596.3 0 64 57 87 596.3 0 87 58 236 596.3 0 236 59 30 596.3 0 30 60 26 596.3 0 26 61 24 596.3 0 24 62 32 596.3 0 32 63 32 596.3 0 32 64 22 596.3 0 22 65 21 596.3 0 21 66 32 596.3 0 32 67 33 596.3 0 33 68 24 596.3 0 24 69 26 596.3 0 26 70 35 596.3 0 35 71 90 596.3 0 90 72 60 596.3 0 60 73 150 596.3 0 150 74 24 596.3 0 24 75 28 596.3 0 28 76 22 596.3 0 22 77 23 596.3 0 23 78 37 596.3 0 37 79 41 596.3 0 41 80 18 596.3 0 18 81 28 596.3 0 28 82 33 596.3 0 33 83 44 596.3 0 44 84 40 596.3 0 40 85 48 596.3 0 48 86 65 596.3 0 65 87 111 596.3 0 111 88 70 596.3 0 70 89 106 596.3 0 106 90 396 596.3 0 396 91 150 596.3 0 150 92 28 596.3 0 28 93 29 596.3 0 29 94 22 596.3 0 22 95 25 596.3 0 25 96 25 596.3 0 25 97 24 596.3 0 24 98 38 596.3 0 38 99 39 596.3 0 39 100 31 596.3 0 31 101 45 596.3 0 45 102 51 596.3 0 51 103 273 596.3 0 273 104 326 596.3 0 326 105 376 596.3 0 376 106 224 596.3 0 224 107 93 596.3 0 93 108 129 596.3 0 129 109 66 596.3 0 66 110 24 596.3 0 24 111 51 596.3 0 51 112 54 596.3 0 54 113 70 596.3 0 70 114 48 596.3 0 48 115 42 596.3 0 42 116 57 596.3 0 57 117 38 596.3 0 38 118 48 596.3 0 48 119 107 596.3 0 107 120 62 596.3 0 62 121 73 596.3 0 73 122 40 596.3 0 40 123 105 596.3 0 105 124 41 596.3 0 41 125 38 596.3 0 38 126 60 596.3 0 60 127 148 596.3 0 148 128 192 596.3 0 192 129 328 596.3 0 328 130 85 596.3 0 85 131 146 596.3 0 146 132 176 596.3 0 176 133 169 596.3 0 169 134 272 596.3 0 272 135 152 596.3 0 152 136 177 596.3 0 177 137 192 596.3 0 192 138 216 596.3 0 216 139 357 596.3 0 357 140 430 596.3 0 430 141 502 596.3 0 502 142 483 596.3 0 483 143 601 596.3 0 601 144 561 596.3 0 561 145 899 596.3 0 899 146 9702 596.3 0 9702 147 8168 596.3 0 8168 148 15970 596.3 0 15970 149 632 596.3 0 632 150 7991 596.3 0 7991 151 3460 596.3 0 3460 152 14439 596.3 0 14439 153 100 596.3 0 100 154 72 596.3 0 72 155 94 596.3 0 94 156 472 596.3 0 472 157 327 596.3 0 327 158 428 596.3 0 428 159 398 596.3 0 398 160 226 596.3 0 226 161 260 596.3 0 260 162 227 596.3 0 227 163 68 596.3 0 68 164 49 596.3 0 49 165 52 596.3 0 52 166 82 596.3 0 82 167 64 596.3 0 64 168 61 596.3 0 61 169 45 596.3 0 45 170 59 596.3 0 59 171 36 596.3 0 36 172 31 596.3 0 31 173 42 596.3 0 42 174 48 596.3 0 48 175 189 596.3 0 189 176 431 596.3 0 431 177 124 596.3 0 124 178 45 596.3 0 45 179 57 596.3 0 57 180 56 596.3 0 56 181 150 596.3 0 150 182 134 596.3 0 134 183 145 596.3 0 145 184 120 596.3 0 120 185 157 596.3 0 157 186 228 596.3 0 228 187 124 596.3 0 124 188 106 596.3 0 106 189 53 596.3 0 53 190 37 596.3 0 37 191 37 596.3 0 37 192 44 596.3 0 44 193 33 596.3 0 33 194 42 596.3 0 42 195 66 596.3 0 66 196 47 596.3 0 47 197 36 596.3 0 36 198 33 596.3 0 33 199 87 596.3 0 87 200 77 596.3 0 77 201 70 596.3 0 70 202 76 596.3 0 76 203 102 596.3 0 102 204 170 596.3 0 170 205 138 596.3 0 138 206 121 596.3 0 121 207 100 596.3 0 100 208 221 596.3 0 221 209 119 596.3 0 119 210 102 596.3 0 102 211 97 596.3 0 97 212 278 596.3 0 278 213 186 596.3 0 186 214 177 596.3 0 177 215 77 596.3 0 77 216 72 596.3 0 72 217 40 596.3 0 40 218 55 596.3 0 55 219 92 596.3 0 92 220 232 596.3 0 232 221 166 596.3 0 166 222 237 596.3 0 237 223 73 596.3 0 73 224 103 596.3 0 103 225 189 596.3 0 189 226 112 596.3 0 112 227 63 596.3 0 63 228 67 596.3 0 67 229 88 596.3 0 88 230 75 596.3 0 75 231 79 596.3 0 79 232 81 596.3 0 81 233 78 596.3 0 78 234 62 596.3 0 62 235 132 596.3 0 132 236 150 596.3 0 150 237 101 596.3 0 101 238 144 596.3 0 144 239 136 596.3 0 136 240 88 596.3 0 88 241 120 596.3 0 120 242 111 596.3 0 111 243 138 596.3 0 138 244 185 596.3 0 185 245 151 596.3 0 151 246 120 596.3 0 120 247 86 596.3 0 86 248 77 596.3 0 77 249 130 596.3 0 130 250 135 596.3 0 135 251 179 596.3 0 179 252 256 596.3 0 256 253 296 596.3 0 296 254 498 596.3 0 498 255 998 596.3 0 998 256 9518 596.3 0 9518 257 8732 596.3 0 8732 258 13507 596.3 0 13507 259 3314 596.3 0 3314 260 205 596.3 0 205 261 231 596.3 0 231 262 334 596.3 0 334 263 268 596.3 0 268 264 192 596.3 0 192 265 87 596.3 0 87 266 138 596.3 0 138 267 219 596.3 0 219 268 221 596.3 0 221 269 168 596.3 0 168 270 66 596.3 0 66 271 64 596.3 0 64 272 66 596.3 0 66 273 95 596.3 0 95 274 172 596.3 0 172 275 163 596.3 0 163 276 102 596.3 0 102 277 90 596.3 0 90 278 73 596.3 0 73 279 137 596.3 0 137 280 352 596.3 0 352 281 523 596.3 0 523 282 397 596.3 0 397 283 247 596.3 0 247 284 295 596.3 0 295 285 293 596.3 0 293 286 129 596.3 0 129 287 93 596.3 0 93 288 97 596.3 0 97 289 185 596.3 0 185 290 128 596.3 0 128 291 67 596.3 0 67 292 68 596.3 0 68 293 117 596.3 0 117 294 91 596.3 0 91 295 89 596.3 0 89 296 118 596.3 0 118 297 101 596.3 0 101 298 98 596.3 0 98 299 79 596.3 0 79 300 80 596.3 0 80 301 148 596.3 0 148 Finished in 10.12 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 244,518 Reads with adapters: 87,455 (35.8%) Reads that were too short: 262 (0.1%) Reads written (passing filters): 87,193 (35.7%) Total basepairs processed: 60,895,710 bp Total written (filtered): 23,970,470 bp (39.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 87455 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 10 0.0 2 0 0 0 10 15 69 0.0 3 0 0 47 22 16 972 0.0 3 0 852 88 32 17 85530 0.0 3 79896 4683 765 186 18 166 0.0 3 14 120 16 16 19 197 0.0 3 0 0 118 79 20 167 0.0 3 0 2 0 165 21 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 27 1 0.0 3 0 1 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 33 9 0.0 3 0 0 0 9 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 36 4 0.0 3 0 0 0 4 38 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 77 6 0.0 3 0 0 0 6 82 2 0.0 3 0 0 0 2 83 3 0.0 3 0 0 3 84 2 0.0 3 0 0 0 2 85 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 103 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 3 0.0 3 0 0 0 3 119 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 7 0.0 3 0 0 0 7 129 1 0.0 3 0 0 0 1 134 2 0.0 3 0 0 0 2 135 1 0.0 3 0 0 1 138 3 0.0 3 0 0 0 3 141 2 0.0 3 0 0 0 2 142 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 3 0.0 3 0 0 0 3 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 155 2 0.0 3 0 0 0 2 156 3 0.0 3 0 0 0 3 163 2 0.0 3 0 0 0 2 164 3 0.0 3 0 0 0 3 165 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 174 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 188 2 0.0 3 0 0 0 2 189 1 0.0 3 0 0 0 1 191 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 0 1 193 2 0.0 3 0 0 0 2 194 3 0.0 3 0 0 0 3 195 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 204 2 0.0 3 0 0 0 2 205 3 0.0 3 0 0 0 3 215 1 0.0 3 0 0 0 1 216 3 0.0 3 0 0 0 3 217 141 0.0 3 0 0 9 132 218 2 0.0 3 0 0 0 2 221 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 1 238 4 0.0 3 0 0 0 4 254 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 8 0.0 3 0 0 0 8 280 11 0.0 3 0 0 0 11 281 11 0.0 3 0 0 0 11 282 12 0.0 3 0 0 0 12 283 4 0.0 3 0 0 0 4 285 1 0.0 3 0 0 0 1 288 2 0.0 3 0 0 0 2 289 2 0.0 3 0 0 0 2 293 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.05 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 87,193 Reads with adapters: 3,104 (3.6%) Reads written (passing filters): 87,193 (100.0%) Total basepairs processed: 23,970,470 bp Total written (filtered): 23,961,029 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 3104 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 57.7% C: 15.3% G: 23.8% T: 3.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3000 1362.4 0 3000 4 95 340.6 0 95 5 5 85.1 0 5 6 2 21.3 0 2 12 2 0.0 1 0 2