This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TACTTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.05 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 285,388 (11.7%) Reads that were too short: 26,280 (1.1%) Reads written (passing filters): 259,108 (10.6%) Total basepairs processed: 735,190,694 bp Total written (filtered): 64,038,010 bp (8.7%) === Adapter 1 === Sequence: TACTTC; Type: regular 5'; Length: 6; Trimmed: 285388 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 9390 596.3 0 9390 7 85480 596.3 0 85480 8 836 596.3 0 836 9 76759 596.3 0 76759 10 45 596.3 0 45 11 21 596.3 0 21 12 30 596.3 0 30 13 12 596.3 0 12 14 15 596.3 0 15 15 13 596.3 0 13 16 23 596.3 0 23 17 31 596.3 0 31 18 23 596.3 0 23 19 23 596.3 0 23 20 12 596.3 0 12 21 20 596.3 0 20 22 11 596.3 0 11 23 21 596.3 0 21 24 26 596.3 0 26 25 23 596.3 0 23 26 26 596.3 0 26 27 15 596.3 0 15 28 20 596.3 0 20 29 22 596.3 0 22 30 22 596.3 0 22 31 30 596.3 0 30 32 22 596.3 0 22 33 18 596.3 0 18 34 25 596.3 0 25 35 17 596.3 0 17 36 22 596.3 0 22 37 23 596.3 0 23 38 26 596.3 0 26 39 23 596.3 0 23 40 19 596.3 0 19 41 24 596.3 0 24 42 17 596.3 0 17 43 25 596.3 0 25 44 18 596.3 0 18 45 14 596.3 0 14 46 15 596.3 0 15 47 29 596.3 0 29 48 11 596.3 0 11 49 7 596.3 0 7 50 19 596.3 0 19 51 26 596.3 0 26 52 25 596.3 0 25 53 20 596.3 0 20 54 25 596.3 0 25 55 18 596.3 0 18 56 58 596.3 0 58 57 56 596.3 0 56 58 199 596.3 0 199 59 23 596.3 0 23 60 20 596.3 0 20 61 17 596.3 0 17 62 27 596.3 0 27 63 23 596.3 0 23 64 15 596.3 0 15 65 21 596.3 0 21 66 24 596.3 0 24 67 29 596.3 0 29 68 34 596.3 0 34 69 23 596.3 0 23 70 22 596.3 0 22 71 42 596.3 0 42 72 36 596.3 0 36 73 81 596.3 0 81 74 19 596.3 0 19 75 25 596.3 0 25 76 20 596.3 0 20 77 21 596.3 0 21 78 24 596.3 0 24 79 28 596.3 0 28 80 14 596.3 0 14 81 23 596.3 0 23 82 34 596.3 0 34 83 33 596.3 0 33 84 24 596.3 0 24 85 41 596.3 0 41 86 34 596.3 0 34 87 104 596.3 0 104 88 59 596.3 0 59 89 123 596.3 0 123 90 292 596.3 0 292 91 153 596.3 0 153 92 40 596.3 0 40 93 30 596.3 0 30 94 37 596.3 0 37 95 30 596.3 0 30 96 28 596.3 0 28 97 32 596.3 0 32 98 29 596.3 0 29 99 27 596.3 0 27 100 35 596.3 0 35 101 37 596.3 0 37 102 61 596.3 0 61 103 168 596.3 0 168 104 193 596.3 0 193 105 178 596.3 0 178 106 138 596.3 0 138 107 64 596.3 0 64 108 63 596.3 0 63 109 54 596.3 0 54 110 25 596.3 0 25 111 36 596.3 0 36 112 37 596.3 0 37 113 51 596.3 0 51 114 29 596.3 0 29 115 40 596.3 0 40 116 67 596.3 0 67 117 32 596.3 0 32 118 52 596.3 0 52 119 80 596.3 0 80 120 45 596.3 0 45 121 40 596.3 0 40 122 53 596.3 0 53 123 102 596.3 0 102 124 36 596.3 0 36 125 35 596.3 0 35 126 43 596.3 0 43 127 78 596.3 0 78 128 118 596.3 0 118 129 253 596.3 0 253 130 62 596.3 0 62 131 131 596.3 0 131 132 154 596.3 0 154 133 189 596.3 0 189 134 273 596.3 0 273 135 111 596.3 0 111 136 132 596.3 0 132 137 149 596.3 0 149 138 160 596.3 0 160 139 248 596.3 0 248 140 293 596.3 0 293 141 322 596.3 0 322 142 384 596.3 0 384 143 642 596.3 0 642 144 560 596.3 0 560 145 929 596.3 0 929 146 12004 596.3 0 12004 147 10044 596.3 0 10044 148 19334 596.3 0 19334 149 677 596.3 0 677 150 9426 596.3 0 9426 151 3872 596.3 0 3872 152 17683 596.3 0 17683 153 100 596.3 0 100 154 60 596.3 0 60 155 63 596.3 0 63 156 388 596.3 0 388 157 244 596.3 0 244 158 255 596.3 0 255 159 264 596.3 0 264 160 151 596.3 0 151 161 141 596.3 0 141 162 128 596.3 0 128 163 48 596.3 0 48 164 34 596.3 0 34 165 32 596.3 0 32 166 40 596.3 0 40 167 40 596.3 0 40 168 47 596.3 0 47 169 39 596.3 0 39 170 34 596.3 0 34 171 25 596.3 0 25 172 24 596.3 0 24 173 31 596.3 0 31 174 33 596.3 0 33 175 92 596.3 0 92 176 141 596.3 0 141 177 57 596.3 0 57 178 27 596.3 0 27 179 31 596.3 0 31 180 40 596.3 0 40 181 63 596.3 0 63 182 64 596.3 0 64 183 47 596.3 0 47 184 62 596.3 0 62 185 64 596.3 0 64 186 105 596.3 0 105 187 65 596.3 0 65 188 58 596.3 0 58 189 38 596.3 0 38 190 36 596.3 0 36 191 25 596.3 0 25 192 30 596.3 0 30 193 26 596.3 0 26 194 31 596.3 0 31 195 47 596.3 0 47 196 30 596.3 0 30 197 25 596.3 0 25 198 26 596.3 0 26 199 37 596.3 0 37 200 36 596.3 0 36 201 51 596.3 0 51 202 67 596.3 0 67 203 67 596.3 0 67 204 65 596.3 0 65 205 58 596.3 0 58 206 48 596.3 0 48 207 51 596.3 0 51 208 127 596.3 0 127 209 71 596.3 0 71 210 53 596.3 0 53 211 57 596.3 0 57 212 223 596.3 0 223 213 99 596.3 0 99 214 90 596.3 0 90 215 49 596.3 0 49 216 54 596.3 0 54 217 49 596.3 0 49 218 34 596.3 0 34 219 61 596.3 0 61 220 135 596.3 0 135 221 100 596.3 0 100 222 121 596.3 0 121 223 60 596.3 0 60 224 71 596.3 0 71 225 145 596.3 0 145 226 72 596.3 0 72 227 41 596.3 0 41 228 46 596.3 0 46 229 53 596.3 0 53 230 67 596.3 0 67 231 56 596.3 0 56 232 56 596.3 0 56 233 69 596.3 0 69 234 66 596.3 0 66 235 90 596.3 0 90 236 92 596.3 0 92 237 65 596.3 0 65 238 94 596.3 0 94 239 92 596.3 0 92 240 73 596.3 0 73 241 80 596.3 0 80 242 59 596.3 0 59 243 78 596.3 0 78 244 92 596.3 0 92 245 68 596.3 0 68 246 66 596.3 0 66 247 50 596.3 0 50 248 60 596.3 0 60 249 83 596.3 0 83 250 89 596.3 0 89 251 114 596.3 0 114 252 145 596.3 0 145 253 142 596.3 0 142 254 247 596.3 0 247 255 461 596.3 0 461 256 4192 596.3 0 4192 257 4044 596.3 0 4044 258 7286 596.3 0 7286 259 1999 596.3 0 1999 260 105 596.3 0 105 261 101 596.3 0 101 262 170 596.3 0 170 263 126 596.3 0 126 264 94 596.3 0 94 265 73 596.3 0 73 266 68 596.3 0 68 267 78 596.3 0 78 268 114 596.3 0 114 269 75 596.3 0 75 270 37 596.3 0 37 271 57 596.3 0 57 272 42 596.3 0 42 273 46 596.3 0 46 274 94 596.3 0 94 275 118 596.3 0 118 276 59 596.3 0 59 277 58 596.3 0 58 278 48 596.3 0 48 279 95 596.3 0 95 280 218 596.3 0 218 281 299 596.3 0 299 282 253 596.3 0 253 283 142 596.3 0 142 284 93 596.3 0 93 285 121 596.3 0 121 286 60 596.3 0 60 287 78 596.3 0 78 288 74 596.3 0 74 289 132 596.3 0 132 290 90 596.3 0 90 291 27 596.3 0 27 292 51 596.3 0 51 293 79 596.3 0 79 294 70 596.3 0 70 295 96 596.3 0 96 296 94 596.3 0 94 297 54 596.3 0 54 298 70 596.3 0 70 299 56 596.3 0 56 300 50 596.3 0 50 301 73 596.3 0 73 Finished in 11.69 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 259,108 Reads with adapters: 96,118 (37.1%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 96,100 (37.1%) Total basepairs processed: 64,038,010 bp Total written (filtered): 26,125,558 bp (40.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 96118 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 36 0.0 3 0 0 35 1 19 913 0.0 3 0 899 12 1 1 20 92895 0.0 4 91141 1685 62 7 21 217 0.0 4 26 187 3 0 1 22 11 0.0 4 1 0 9 1 23 55 0.0 4 0 0 2 43 10 24 5 0.0 4 2 0 0 0 3 25 1 0.0 4 0 0 0 1 28 2 0.0 4 0 0 0 0 2 34 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 45 1 0.0 4 1 61 1 0.0 4 0 0 0 0 1 72 3 0.0 4 2 1 75 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 104 1 0.0 4 1 121 1 0.0 4 1 125 2 0.0 4 0 0 0 0 2 126 33 0.0 4 0 0 0 0 33 127 1910 0.0 4 0 0 0 0 1910 129 1 0.0 4 0 0 0 0 1 130 4 0.0 4 0 0 0 0 4 135 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 1 209 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 1 233 2 0.0 4 0 0 0 0 2 234 1 0.0 4 0 0 0 0 1 260 2 0.0 4 0 0 0 0 2 263 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.30 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 96,100 Reads with adapters: 2,865 (3.0%) Reads written (passing filters): 96,100 (100.0%) Total basepairs processed: 26,125,558 bp Total written (filtered): 25,992,202 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2865 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 6.2% C: 4.3% G: 73.9% T: 8.3% none/other: 7.3% Overview of removed sequences length count expect max.err error counts 3 204 1501.6 0 204 4 15 375.4 0 15 5 6 93.8 0 6 8 1 1.5 0 1 9 9 0.4 0 1 8 10 24 0.1 1 0 24 11 1932 0.0 1 15 1917 12 258 0.0 1 1 257 13 10 0.0 1 0 10 14 3 0.0 1 1 2 15 2 0.0 1 0 2 34 1 0.0 1 1 44 1 0.0 1 0 1 55 1 0.0 1 1 76 1 0.0 1 1 152 2 0.0 1 0 2 153 1 0.0 1 1 172 2 0.0 1 2 225 9 0.0 1 9 264 1 0.0 1 1 271 30 0.0 1 27 3 272 4 0.0 1 4 273 118 0.0 1 111 7 274 206 0.0 1 195 11 275 24 0.0 1 22 2