This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCTCCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.54 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 118,655 (4.9%) Reads that were too short: 21,139 (0.9%) Reads written (passing filters): 97,516 (4.0%) Total basepairs processed: 735,190,694 bp Total written (filtered): 21,061,578 bp (2.9%) === Adapter 1 === Sequence: GCTCCG; Type: regular 5'; Length: 6; Trimmed: 118655 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1969 596.3 0 1969 7 22444 596.3 0 22444 8 932 596.3 0 932 9 19723 596.3 0 19723 10 37 596.3 0 37 11 49 596.3 0 49 12 14 596.3 0 14 13 27 596.3 0 27 14 9 596.3 0 9 15 30 596.3 0 30 16 15 596.3 0 15 17 16 596.3 0 16 18 17 596.3 0 17 19 15 596.3 0 15 20 13 596.3 0 13 21 18 596.3 0 18 22 18 596.3 0 18 23 30 596.3 0 30 24 20 596.3 0 20 25 20 596.3 0 20 26 21 596.3 0 21 27 20 596.3 0 20 28 14 596.3 0 14 29 16 596.3 0 16 30 35 596.3 0 35 31 12 596.3 0 12 32 15 596.3 0 15 33 18 596.3 0 18 34 15 596.3 0 15 35 20 596.3 0 20 36 17 596.3 0 17 37 13 596.3 0 13 38 28 596.3 0 28 39 18 596.3 0 18 40 126 596.3 0 126 41 20 596.3 0 20 42 74 596.3 0 74 43 19 596.3 0 19 44 15 596.3 0 15 45 15 596.3 0 15 46 27 596.3 0 27 47 17 596.3 0 17 48 10 596.3 0 10 49 27 596.3 0 27 50 28 596.3 0 28 51 16 596.3 0 16 52 16 596.3 0 16 53 23 596.3 0 23 54 19 596.3 0 19 55 116 596.3 0 116 56 61 596.3 0 61 57 147 596.3 0 147 58 30 596.3 0 30 59 35 596.3 0 35 60 39 596.3 0 39 61 38 596.3 0 38 62 25 596.3 0 25 63 9 596.3 0 9 64 39 596.3 0 39 65 27 596.3 0 27 66 44 596.3 0 44 67 26 596.3 0 26 68 54 596.3 0 54 69 20 596.3 0 20 70 20 596.3 0 20 71 45 596.3 0 45 72 30 596.3 0 30 73 30 596.3 0 30 74 12 596.3 0 12 75 87 596.3 0 87 76 20 596.3 0 20 77 22 596.3 0 22 78 21 596.3 0 21 79 19 596.3 0 19 80 15 596.3 0 15 81 13 596.3 0 13 82 25 596.3 0 25 83 32 596.3 0 32 84 63 596.3 0 63 85 33 596.3 0 33 86 29 596.3 0 29 87 27 596.3 0 27 88 31 596.3 0 31 89 40 596.3 0 40 90 51 596.3 0 51 91 57 596.3 0 57 92 43 596.3 0 43 93 34 596.3 0 34 94 26 596.3 0 26 95 34 596.3 0 34 96 17 596.3 0 17 97 26 596.3 0 26 98 23 596.3 0 23 99 8 596.3 0 8 100 19 596.3 0 19 101 28 596.3 0 28 102 29 596.3 0 29 103 66 596.3 0 66 104 60 596.3 0 60 105 306 596.3 0 306 106 291 596.3 0 291 107 147 596.3 0 147 108 21 596.3 0 21 109 21 596.3 0 21 110 31 596.3 0 31 111 32 596.3 0 32 112 24 596.3 0 24 113 28 596.3 0 28 114 50 596.3 0 50 115 158 596.3 0 158 116 30 596.3 0 30 117 35 596.3 0 35 118 30 596.3 0 30 119 17 596.3 0 17 120 25 596.3 0 25 121 35 596.3 0 35 122 28 596.3 0 28 123 58 596.3 0 58 124 28 596.3 0 28 125 47 596.3 0 47 126 61 596.3 0 61 127 316 596.3 0 316 128 55 596.3 0 55 129 188 596.3 0 188 130 96 596.3 0 96 131 48 596.3 0 48 132 135 596.3 0 135 133 137 596.3 0 137 134 211 596.3 0 211 135 80 596.3 0 80 136 85 596.3 0 85 137 183 596.3 0 183 138 79 596.3 0 79 139 88 596.3 0 88 140 146 596.3 0 146 141 128 596.3 0 128 142 248 596.3 0 248 143 193 596.3 0 193 144 242 596.3 0 242 145 322 596.3 0 322 146 442 596.3 0 442 147 7646 596.3 0 7646 148 11771 596.3 0 11771 149 11365 596.3 0 11365 150 117 596.3 0 117 151 80 596.3 0 80 152 192 596.3 0 192 153 204 596.3 0 204 154 391 596.3 0 391 155 296 596.3 0 296 156 84 596.3 0 84 157 64 596.3 0 64 158 87 596.3 0 87 159 68 596.3 0 68 160 30 596.3 0 30 161 42 596.3 0 42 162 38 596.3 0 38 163 54 596.3 0 54 164 53 596.3 0 53 165 42 596.3 0 42 166 38 596.3 0 38 167 104 596.3 0 104 168 426 596.3 0 426 169 100 596.3 0 100 170 200 596.3 0 200 171 58 596.3 0 58 172 114 596.3 0 114 173 195 596.3 0 195 174 165 596.3 0 165 175 128 596.3 0 128 176 94 596.3 0 94 177 72 596.3 0 72 178 129 596.3 0 129 179 102 596.3 0 102 180 37 596.3 0 37 181 38 596.3 0 38 182 59 596.3 0 59 183 67 596.3 0 67 184 99 596.3 0 99 185 69 596.3 0 69 186 94 596.3 0 94 187 182 596.3 0 182 188 1876 596.3 0 1876 189 975 596.3 0 975 190 2634 596.3 0 2634 191 94 596.3 0 94 192 37 596.3 0 37 193 30 596.3 0 30 194 44 596.3 0 44 195 73 596.3 0 73 196 130 596.3 0 130 197 112 596.3 0 112 198 122 596.3 0 122 199 197 596.3 0 197 200 293 596.3 0 293 201 2805 596.3 0 2805 202 1878 596.3 0 1878 203 2959 596.3 0 2959 204 600 596.3 0 600 205 94 596.3 0 94 206 121 596.3 0 121 207 79 596.3 0 79 208 57 596.3 0 57 209 44 596.3 0 44 210 42 596.3 0 42 211 15 596.3 0 15 212 21 596.3 0 21 213 24 596.3 0 24 214 55 596.3 0 55 215 87 596.3 0 87 216 53 596.3 0 53 217 58 596.3 0 58 218 22 596.3 0 22 219 25 596.3 0 25 220 47 596.3 0 47 221 50 596.3 0 50 222 32 596.3 0 32 223 44 596.3 0 44 224 73 596.3 0 73 225 127 596.3 0 127 226 107 596.3 0 107 227 102 596.3 0 102 228 321 596.3 0 321 229 167 596.3 0 167 230 475 596.3 0 475 231 94 596.3 0 94 232 58 596.3 0 58 233 37 596.3 0 37 234 79 596.3 0 79 235 91 596.3 0 91 236 87 596.3 0 87 237 43 596.3 0 43 238 65 596.3 0 65 239 42 596.3 0 42 240 73 596.3 0 73 241 108 596.3 0 108 242 38 596.3 0 38 243 102 596.3 0 102 244 99 596.3 0 99 245 119 596.3 0 119 246 49 596.3 0 49 247 80 596.3 0 80 248 93 596.3 0 93 249 154 596.3 0 154 250 175 596.3 0 175 251 129 596.3 0 129 252 121 596.3 0 121 253 94 596.3 0 94 254 59 596.3 0 59 255 30 596.3 0 30 256 45 596.3 0 45 257 38 596.3 0 38 258 88 596.3 0 88 259 118 596.3 0 118 260 127 596.3 0 127 261 101 596.3 0 101 262 110 596.3 0 110 263 87 596.3 0 87 264 83 596.3 0 83 265 64 596.3 0 64 266 72 596.3 0 72 267 54 596.3 0 54 268 71 596.3 0 71 269 422 596.3 0 422 270 468 596.3 0 468 271 463 596.3 0 463 272 173 596.3 0 173 273 73 596.3 0 73 274 93 596.3 0 93 275 196 596.3 0 196 276 177 596.3 0 177 277 150 596.3 0 150 278 95 596.3 0 95 279 101 596.3 0 101 280 206 596.3 0 206 281 286 596.3 0 286 282 500 596.3 0 500 283 337 596.3 0 337 284 216 596.3 0 216 285 164 596.3 0 164 286 302 596.3 0 302 287 539 596.3 0 539 288 353 596.3 0 353 289 107 596.3 0 107 290 108 596.3 0 108 291 347 596.3 0 347 292 496 596.3 0 496 293 777 596.3 0 777 294 1053 596.3 0 1053 295 600 596.3 0 600 296 764 596.3 0 764 297 261 596.3 0 261 298 72 596.3 0 72 299 85 596.3 0 85 300 107 596.3 0 107 301 92 596.3 0 92 Finished in 4.03 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 97,516 Reads with adapters: 23,893 (24.5%) Reads that were too short: 159 (0.2%) Reads written (passing filters): 23,734 (24.3%) Total basepairs processed: 21,061,578 bp Total written (filtered): 6,531,639 bp (31.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 23893 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 6 0.0 2 0 0 0 6 15 12 0.0 3 0 0 2 10 16 191 0.0 3 0 150 20 21 17 22421 0.0 3 20908 1268 191 54 18 91 0.0 3 10 68 6 7 19 755 0.0 3 548 39 52 116 20 67 0.0 3 0 0 0 67 21 2 0.0 3 0 0 0 2 22 18 0.0 3 0 0 1 17 25 1 0.0 3 0 0 0 1 27 5 0.0 3 0 0 0 5 28 2 0.0 3 0 0 0 2 32 1 0.0 3 0 0 0 1 35 4 0.0 3 0 0 0 4 37 1 0.0 3 0 0 0 1 39 2 0.0 3 0 0 0 2 40 4 0.0 3 0 0 0 4 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 44 0.0 3 0 0 1 43 45 71 0.0 3 0 0 0 71 46 3 0.0 3 0 0 0 3 56 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 9 0.0 3 0 0 0 9 66 2 0.0 3 0 0 0 2 67 2 0.0 3 0 0 0 2 68 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 70 11 0.0 3 0 1 0 10 77 1 0.0 3 0 0 0 1 79 27 0.0 3 0 0 0 27 80 1 0.0 3 0 0 0 1 81 1 0.0 3 1 82 3 0.0 3 0 0 0 3 86 4 0.0 3 0 0 0 4 87 15 0.0 3 0 0 0 15 89 1 0.0 3 0 0 0 1 90 4 0.0 3 0 0 0 4 91 2 0.0 3 1 0 0 1 92 3 0.0 3 0 0 0 3 98 6 0.0 3 0 0 0 6 99 2 0.0 3 0 0 0 2 100 3 0.0 3 0 0 0 3 101 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 7 0.0 3 0 0 1 6 123 2 0.0 3 0 0 0 2 124 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 131 3 0.0 3 0 0 0 3 132 2 0.0 3 0 0 0 2 134 2 0.0 3 0 0 1 1 136 1 0.0 3 0 0 1 137 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 0 2 147 3 0.0 3 0 0 1 2 148 1 0.0 3 0 1 150 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 3 0.0 3 0 0 0 3 165 1 0.0 3 0 0 0 1 166 2 0.0 3 0 0 0 2 167 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 202 1 0.0 3 0 0 1 204 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 234 2 0.0 3 0 0 0 2 236 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 278 2 0.0 3 0 0 0 2 279 5 0.0 3 0 0 0 5 280 4 0.0 3 0 0 0 4 281 2 0.0 3 0 0 0 2 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.84 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 23,734 Reads with adapters: 187 (0.8%) Reads written (passing filters): 23,734 (100.0%) Total basepairs processed: 6,531,639 bp Total written (filtered): 6,531,059 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 187 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 25.7% C: 41.7% G: 21.9% T: 10.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 175 370.8 0 175 4 8 92.7 0 8 5 1 23.2 0 1 6 3 5.8 0 3