This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCTCCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.32 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 101,250 (4.1%) Reads that were too short: 11,035 (0.5%) Reads written (passing filters): 90,215 (3.7%) Total basepairs processed: 735,190,694 bp Total written (filtered): 20,133,036 bp (2.7%) === Adapter 1 === Sequence: GCTCCG; Type: regular 5'; Length: 6; Trimmed: 101250 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1599 596.3 0 1599 7 20163 596.3 0 20163 8 659 596.3 0 659 9 24145 596.3 0 24145 10 41 596.3 0 41 11 13 596.3 0 13 12 4 596.3 0 4 13 10 596.3 0 10 14 5 596.3 0 5 15 13 596.3 0 13 16 6 596.3 0 6 17 6 596.3 0 6 18 6 596.3 0 6 19 9 596.3 0 9 20 13 596.3 0 13 21 11 596.3 0 11 22 11 596.3 0 11 23 8 596.3 0 8 24 7 596.3 0 7 25 7 596.3 0 7 26 5 596.3 0 5 27 8 596.3 0 8 28 7 596.3 0 7 29 8 596.3 0 8 30 12 596.3 0 12 31 12 596.3 0 12 32 13 596.3 0 13 33 12 596.3 0 12 34 7 596.3 0 7 35 7 596.3 0 7 36 8 596.3 0 8 37 15 596.3 0 15 38 20 596.3 0 20 39 9 596.3 0 9 40 24 596.3 0 24 41 13 596.3 0 13 42 9 596.3 0 9 43 7 596.3 0 7 44 13 596.3 0 13 45 13 596.3 0 13 46 17 596.3 0 17 47 9 596.3 0 9 48 7 596.3 0 7 49 10 596.3 0 10 50 8 596.3 0 8 51 8 596.3 0 8 52 5 596.3 0 5 53 12 596.3 0 12 54 7 596.3 0 7 55 68 596.3 0 68 56 31 596.3 0 31 57 73 596.3 0 73 58 12 596.3 0 12 59 16 596.3 0 16 60 12 596.3 0 12 61 9 596.3 0 9 62 12 596.3 0 12 63 12 596.3 0 12 64 10 596.3 0 10 65 20 596.3 0 20 66 8 596.3 0 8 67 9 596.3 0 9 68 13 596.3 0 13 69 11 596.3 0 11 70 15 596.3 0 15 71 12 596.3 0 12 72 11 596.3 0 11 73 12 596.3 0 12 74 12 596.3 0 12 75 61 596.3 0 61 76 22 596.3 0 22 77 19 596.3 0 19 78 12 596.3 0 12 79 14 596.3 0 14 80 11 596.3 0 11 81 18 596.3 0 18 82 10 596.3 0 10 83 14 596.3 0 14 84 19 596.3 0 19 85 14 596.3 0 14 86 31 596.3 0 31 87 15 596.3 0 15 88 20 596.3 0 20 89 26 596.3 0 26 90 22 596.3 0 22 91 19 596.3 0 19 92 16 596.3 0 16 93 16 596.3 0 16 94 10 596.3 0 10 95 16 596.3 0 16 96 22 596.3 0 22 97 19 596.3 0 19 98 13 596.3 0 13 99 11 596.3 0 11 100 10 596.3 0 10 101 9 596.3 0 9 102 23 596.3 0 23 103 46 596.3 0 46 104 24 596.3 0 24 105 92 596.3 0 92 106 123 596.3 0 123 107 67 596.3 0 67 108 13 596.3 0 13 109 16 596.3 0 16 110 8 596.3 0 8 111 10 596.3 0 10 112 18 596.3 0 18 113 23 596.3 0 23 114 18 596.3 0 18 115 120 596.3 0 120 116 18 596.3 0 18 117 23 596.3 0 23 118 22 596.3 0 22 119 17 596.3 0 17 120 15 596.3 0 15 121 18 596.3 0 18 122 17 596.3 0 17 123 41 596.3 0 41 124 14 596.3 0 14 125 27 596.3 0 27 126 31 596.3 0 31 127 269 596.3 0 269 128 37 596.3 0 37 129 170 596.3 0 170 130 64 596.3 0 64 131 52 596.3 0 52 132 91 596.3 0 91 133 94 596.3 0 94 134 192 596.3 0 192 135 79 596.3 0 79 136 69 596.3 0 69 137 139 596.3 0 139 138 73 596.3 0 73 139 70 596.3 0 70 140 159 596.3 0 159 141 101 596.3 0 101 142 169 596.3 0 169 143 173 596.3 0 173 144 216 596.3 0 216 145 221 596.3 0 221 146 311 596.3 0 311 147 7240 596.3 0 7240 148 11423 596.3 0 11423 149 11030 596.3 0 11030 150 90 596.3 0 90 151 46 596.3 0 46 152 96 596.3 0 96 153 63 596.3 0 63 154 116 596.3 0 116 155 112 596.3 0 112 156 48 596.3 0 48 157 23 596.3 0 23 158 33 596.3 0 33 159 25 596.3 0 25 160 20 596.3 0 20 161 14 596.3 0 14 162 16 596.3 0 16 163 17 596.3 0 17 164 26 596.3 0 26 165 17 596.3 0 17 166 30 596.3 0 30 167 73 596.3 0 73 168 396 596.3 0 396 169 69 596.3 0 69 170 143 596.3 0 143 171 35 596.3 0 35 172 50 596.3 0 50 173 67 596.3 0 67 174 107 596.3 0 107 175 50 596.3 0 50 176 65 596.3 0 65 177 35 596.3 0 35 178 51 596.3 0 51 179 43 596.3 0 43 180 19 596.3 0 19 181 25 596.3 0 25 182 31 596.3 0 31 183 38 596.3 0 38 184 71 596.3 0 71 185 35 596.3 0 35 186 55 596.3 0 55 187 91 596.3 0 91 188 1013 596.3 0 1013 189 558 596.3 0 558 190 1558 596.3 0 1558 191 62 596.3 0 62 192 15 596.3 0 15 193 26 596.3 0 26 194 25 596.3 0 25 195 40 596.3 0 40 196 46 596.3 0 46 197 66 596.3 0 66 198 80 596.3 0 80 199 119 596.3 0 119 200 201 596.3 0 201 201 2871 596.3 0 2871 202 1640 596.3 0 1640 203 3715 596.3 0 3715 204 557 596.3 0 557 205 19 596.3 0 19 206 12 596.3 0 12 207 16 596.3 0 16 208 17 596.3 0 17 209 19 596.3 0 19 210 20 596.3 0 20 211 11 596.3 0 11 212 6 596.3 0 6 213 14 596.3 0 14 214 19 596.3 0 19 215 42 596.3 0 42 216 18 596.3 0 18 217 38 596.3 0 38 218 10 596.3 0 10 219 8 596.3 0 8 220 16 596.3 0 16 221 19 596.3 0 19 222 8 596.3 0 8 223 21 596.3 0 21 224 26 596.3 0 26 225 63 596.3 0 63 226 30 596.3 0 30 227 45 596.3 0 45 228 173 596.3 0 173 229 93 596.3 0 93 230 243 596.3 0 243 231 21 596.3 0 21 232 16 596.3 0 16 233 19 596.3 0 19 234 20 596.3 0 20 235 30 596.3 0 30 236 32 596.3 0 32 237 14 596.3 0 14 238 37 596.3 0 37 239 17 596.3 0 17 240 14 596.3 0 14 241 28 596.3 0 28 242 36 596.3 0 36 243 31 596.3 0 31 244 36 596.3 0 36 245 33 596.3 0 33 246 12 596.3 0 12 247 15 596.3 0 15 248 29 596.3 0 29 249 29 596.3 0 29 250 52 596.3 0 52 251 35 596.3 0 35 252 34 596.3 0 34 253 36 596.3 0 36 254 23 596.3 0 23 255 11 596.3 0 11 256 19 596.3 0 19 257 17 596.3 0 17 258 27 596.3 0 27 259 36 596.3 0 36 260 48 596.3 0 48 261 45 596.3 0 45 262 31 596.3 0 31 263 19 596.3 0 19 264 21 596.3 0 21 265 21 596.3 0 21 266 27 596.3 0 27 267 19 596.3 0 19 268 24 596.3 0 24 269 110 596.3 0 110 270 118 596.3 0 118 271 126 596.3 0 126 272 34 596.3 0 34 273 29 596.3 0 29 274 36 596.3 0 36 275 56 596.3 0 56 276 78 596.3 0 78 277 90 596.3 0 90 278 33 596.3 0 33 279 36 596.3 0 36 280 50 596.3 0 50 281 55 596.3 0 55 282 133 596.3 0 133 283 100 596.3 0 100 284 78 596.3 0 78 285 47 596.3 0 47 286 160 596.3 0 160 287 276 596.3 0 276 288 130 596.3 0 130 289 21 596.3 0 21 290 43 596.3 0 43 291 131 596.3 0 131 292 175 596.3 0 175 293 233 596.3 0 233 294 271 596.3 0 271 295 140 596.3 0 140 296 136 596.3 0 136 297 66 596.3 0 66 298 18 596.3 0 18 299 30 596.3 0 30 300 28 596.3 0 28 301 36 596.3 0 36 Finished in 4.11 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 90,215 Reads with adapters: 26,245 (29.1%) Reads that were too short: 15 (0.0%) Reads written (passing filters): 26,230 (29.1%) Total basepairs processed: 20,133,036 bp Total written (filtered): 7,087,249 bp (35.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 26245 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 6 0.0 3 0 0 6 19 321 0.0 3 0 312 9 20 24731 0.0 4 24256 453 19 3 21 75 0.0 4 15 55 5 22 580 0.0 4 569 11 23 49 0.0 4 36 0 0 12 1 24 1 0.0 4 1 25 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 1 87 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 112 1 0.0 4 1 125 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 450 0.0 4 0 0 0 0 450 128 3 0.0 4 0 0 0 0 3 129 6 0.0 4 0 0 0 0 6 137 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.93 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 26,230 Reads with adapters: 3,697 (14.1%) Reads written (passing filters): 26,230 (100.0%) Total basepairs processed: 7,087,249 bp Total written (filtered): 7,047,790 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3697 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.0% C: 2.7% G: 94.8% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 5 409.8 0 5 4 3 102.5 0 3 5 1 25.6 0 1 7 1 1.6 0 1 9 1667 0.1 0 34 1633 10 1817 0.0 1 59 1758 11 177 0.0 1 4 173 12 4 0.0 1 0 4 13 3 0.0 1 0 3 14 1 0.0 1 0 1 82 1 0.0 1 1 150 1 0.0 1 0 1 169 1 0.0 1 1 189 1 0.0 1 0 1 223 3 0.0 1 3 267 10 0.0 1 9 1 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.