This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACACAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.29 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 168,124 (6.9%) Reads that were too short: 52,288 (2.1%) Reads written (passing filters): 115,836 (4.7%) Total basepairs processed: 735,190,694 bp Total written (filtered): 29,900,223 bp (4.1%) === Adapter 1 === Sequence: ACACAA; Type: regular 5'; Length: 6; Trimmed: 168124 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 608 596.3 0 608 7 46989 596.3 0 46989 8 419 596.3 0 419 9 36693 596.3 0 36693 10 30 596.3 0 30 11 14 596.3 0 14 12 7 596.3 0 7 13 16 596.3 0 16 14 10 596.3 0 10 15 12 596.3 0 12 16 12 596.3 0 12 17 16 596.3 0 16 18 9 596.3 0 9 19 8 596.3 0 8 20 5 596.3 0 5 21 7 596.3 0 7 22 8 596.3 0 8 23 14 596.3 0 14 24 8 596.3 0 8 25 18 596.3 0 18 26 15 596.3 0 15 27 18 596.3 0 18 28 23 596.3 0 23 29 19 596.3 0 19 30 14 596.3 0 14 31 14 596.3 0 14 32 20 596.3 0 20 33 17 596.3 0 17 34 7 596.3 0 7 35 6 596.3 0 6 36 10 596.3 0 10 37 16 596.3 0 16 38 12 596.3 0 12 39 16 596.3 0 16 40 26 596.3 0 26 41 108 596.3 0 108 42 177 596.3 0 177 43 186 596.3 0 186 44 27 596.3 0 27 45 30 596.3 0 30 46 19 596.3 0 19 47 12 596.3 0 12 48 15 596.3 0 15 49 14 596.3 0 14 50 22 596.3 0 22 51 26 596.3 0 26 52 16 596.3 0 16 53 7 596.3 0 7 54 9 596.3 0 9 55 15 596.3 0 15 56 13 596.3 0 13 57 25 596.3 0 25 58 19 596.3 0 19 59 17 596.3 0 17 60 9 596.3 0 9 61 21 596.3 0 21 62 21 596.3 0 21 63 23 596.3 0 23 64 13 596.3 0 13 65 11 596.3 0 11 66 22 596.3 0 22 67 24 596.3 0 24 68 18 596.3 0 18 69 78 596.3 0 78 70 28 596.3 0 28 71 41 596.3 0 41 72 29 596.3 0 29 73 49 596.3 0 49 74 235 596.3 0 235 75 39 596.3 0 39 76 41 596.3 0 41 77 67 596.3 0 67 78 70 596.3 0 70 79 119 596.3 0 119 80 187 596.3 0 187 81 138 596.3 0 138 82 183 596.3 0 183 83 181 596.3 0 181 84 183 596.3 0 183 85 137 596.3 0 137 86 111 596.3 0 111 87 80 596.3 0 80 88 56 596.3 0 56 89 64 596.3 0 64 90 115 596.3 0 115 91 91 596.3 0 91 92 77 596.3 0 77 93 85 596.3 0 85 94 72 596.3 0 72 95 67 596.3 0 67 96 60 596.3 0 60 97 43 596.3 0 43 98 43 596.3 0 43 99 52 596.3 0 52 100 55 596.3 0 55 101 70 596.3 0 70 102 80 596.3 0 80 103 95 596.3 0 95 104 108 596.3 0 108 105 152 596.3 0 152 106 151 596.3 0 151 107 146 596.3 0 146 108 106 596.3 0 106 109 125 596.3 0 125 110 129 596.3 0 129 111 128 596.3 0 128 112 130 596.3 0 130 113 167 596.3 0 167 114 148 596.3 0 148 115 160 596.3 0 160 116 182 596.3 0 182 117 116 596.3 0 116 118 183 596.3 0 183 119 165 596.3 0 165 120 200 596.3 0 200 121 158 596.3 0 158 122 161 596.3 0 161 123 129 596.3 0 129 124 517 596.3 0 517 125 219 596.3 0 219 126 634 596.3 0 634 127 196 596.3 0 196 128 229 596.3 0 229 129 151 596.3 0 151 130 133 596.3 0 133 131 206 596.3 0 206 132 287 596.3 0 287 133 2416 596.3 0 2416 134 1617 596.3 0 1617 135 3781 596.3 0 3781 136 167 596.3 0 167 137 152 596.3 0 152 138 155 596.3 0 155 139 159 596.3 0 159 140 122 596.3 0 122 141 130 596.3 0 130 142 146 596.3 0 146 143 161 596.3 0 161 144 171 596.3 0 171 145 208 596.3 0 208 146 172 596.3 0 172 147 196 596.3 0 196 148 182 596.3 0 182 149 149 596.3 0 149 150 126 596.3 0 126 151 125 596.3 0 125 152 128 596.3 0 128 153 141 596.3 0 141 154 127 596.3 0 127 155 150 596.3 0 150 156 132 596.3 0 132 157 158 596.3 0 158 158 192 596.3 0 192 159 235 596.3 0 235 160 271 596.3 0 271 161 320 596.3 0 320 162 513 596.3 0 513 163 441 596.3 0 441 164 709 596.3 0 709 165 182 596.3 0 182 166 175 596.3 0 175 167 154 596.3 0 154 168 131 596.3 0 131 169 141 596.3 0 141 170 168 596.3 0 168 171 164 596.3 0 164 172 195 596.3 0 195 173 170 596.3 0 170 174 146 596.3 0 146 175 125 596.3 0 125 176 117 596.3 0 117 177 148 596.3 0 148 178 160 596.3 0 160 179 163 596.3 0 163 180 213 596.3 0 213 181 131 596.3 0 131 182 152 596.3 0 152 183 425 596.3 0 425 184 257 596.3 0 257 185 468 596.3 0 468 186 231 596.3 0 231 187 256 596.3 0 256 188 284 596.3 0 284 189 311 596.3 0 311 190 218 596.3 0 218 191 211 596.3 0 211 192 168 596.3 0 168 193 142 596.3 0 142 194 125 596.3 0 125 195 121 596.3 0 121 196 203 596.3 0 203 197 124 596.3 0 124 198 182 596.3 0 182 199 135 596.3 0 135 200 210 596.3 0 210 201 386 596.3 0 386 202 174 596.3 0 174 203 148 596.3 0 148 204 113 596.3 0 113 205 157 596.3 0 157 206 158 596.3 0 158 207 190 596.3 0 190 208 209 596.3 0 209 209 231 596.3 0 231 210 288 596.3 0 288 211 573 596.3 0 573 212 1134 596.3 0 1134 213 19599 596.3 0 19599 214 3877 596.3 0 3877 215 7753 596.3 0 7753 216 633 596.3 0 633 217 288 596.3 0 288 218 192 596.3 0 192 219 162 596.3 0 162 220 171 596.3 0 171 221 172 596.3 0 172 222 198 596.3 0 198 223 218 596.3 0 218 224 195 596.3 0 195 225 500 596.3 0 500 226 428 596.3 0 428 227 514 596.3 0 514 228 277 596.3 0 277 229 316 596.3 0 316 230 187 596.3 0 187 231 159 596.3 0 159 232 155 596.3 0 155 233 143 596.3 0 143 234 116 596.3 0 116 235 137 596.3 0 137 236 136 596.3 0 136 237 159 596.3 0 159 238 154 596.3 0 154 239 161 596.3 0 161 240 120 596.3 0 120 241 128 596.3 0 128 242 149 596.3 0 149 243 138 596.3 0 138 244 95 596.3 0 95 245 129 596.3 0 129 246 145 596.3 0 145 247 183 596.3 0 183 248 165 596.3 0 165 249 213 596.3 0 213 250 434 596.3 0 434 251 318 596.3 0 318 252 646 596.3 0 646 253 223 596.3 0 223 254 202 596.3 0 202 255 173 596.3 0 173 256 145 596.3 0 145 257 174 596.3 0 174 258 145 596.3 0 145 259 137 596.3 0 137 260 154 596.3 0 154 261 137 596.3 0 137 262 136 596.3 0 136 263 200 596.3 0 200 264 198 596.3 0 198 265 248 596.3 0 248 266 222 596.3 0 222 267 164 596.3 0 164 268 139 596.3 0 139 269 164 596.3 0 164 270 165 596.3 0 165 271 186 596.3 0 186 272 159 596.3 0 159 273 170 596.3 0 170 274 193 596.3 0 193 275 196 596.3 0 196 276 243 596.3 0 243 277 253 596.3 0 253 278 214 596.3 0 214 279 208 596.3 0 208 280 176 596.3 0 176 281 169 596.3 0 169 282 181 596.3 0 181 283 200 596.3 0 200 284 184 596.3 0 184 285 171 596.3 0 171 286 183 596.3 0 183 287 172 596.3 0 172 288 163 596.3 0 163 289 220 596.3 0 220 290 226 596.3 0 226 291 201 596.3 0 201 292 169 596.3 0 169 293 136 596.3 0 136 294 182 596.3 0 182 295 158 596.3 0 158 296 188 596.3 0 188 297 164 596.3 0 164 298 140 596.3 0 140 299 175 596.3 0 175 300 184 596.3 0 184 301 360 596.3 0 360 Finished in 5.72 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 115,836 Reads with adapters: 47,864 (41.3%) Reads that were too short: 15 (0.0%) Reads written (passing filters): 47,849 (41.3%) Total basepairs processed: 29,900,223 bp Total written (filtered): 13,030,517 bp (43.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 47864 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 16 0.0 3 0 0 14 2 19 840 0.0 3 0 824 16 20 46141 0.0 4 45267 834 35 3 2 21 80 0.0 4 16 63 0 1 22 10 0.0 4 6 0 2 1 1 23 25 0.0 4 0 0 0 21 4 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 0 0 0 1 49 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 723 0.0 4 0 0 0 0 723 128 4 0.0 4 0 0 0 0 4 130 2 0.0 4 0 0 0 0 2 140 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 1 199 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.66 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 47,849 Reads with adapters: 1,483 (3.1%) Reads written (passing filters): 47,849 (100.0%) Total basepairs processed: 13,030,517 bp Total written (filtered): 13,013,485 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1483 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.8% C: 4.8% G: 86.6% T: 2.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 79 747.6 0 79 4 9 186.9 0 9 5 7 46.7 0 7 7 3 2.9 0 3 9 8 0.2 0 4 4 10 9 0.0 1 0 9 11 1283 0.0 1 10 1273 12 68 0.0 1 0 68 13 3 0.0 1 0 3 15 2 0.0 1 0 2 20 5 0.0 1 5 63 1 0.0 1 1 210 1 0.0 1 1 225 4 0.0 1 4 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.