This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.51 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 362,345 (14.8%) Reads that were too short: 314,050 (12.9%) Reads written (passing filters): 48,295 (2.0%) Total basepairs processed: 735,190,694 bp Total written (filtered): 10,659,608 bp (1.4%) === Adapter 1 === Sequence: GCGGTT; Type: regular 5'; Length: 6; Trimmed: 362345 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 272 596.3 0 272 7 507 596.3 0 507 8 14110 596.3 0 14110 9 10171 596.3 0 10171 10 41 596.3 0 41 11 34 596.3 0 34 12 19 596.3 0 19 13 34 596.3 0 34 14 38 596.3 0 38 15 19 596.3 0 19 16 31 596.3 0 31 17 24 596.3 0 24 18 30 596.3 0 30 19 21 596.3 0 21 20 101 596.3 0 101 21 65 596.3 0 65 22 107 596.3 0 107 23 43 596.3 0 43 24 27 596.3 0 27 25 22 596.3 0 22 26 30 596.3 0 30 27 26 596.3 0 26 28 38 596.3 0 38 29 30 596.3 0 30 30 24 596.3 0 24 31 25 596.3 0 25 32 15 596.3 0 15 33 94 596.3 0 94 34 44 596.3 0 44 35 50 596.3 0 50 36 24 596.3 0 24 37 20 596.3 0 20 38 32 596.3 0 32 39 27 596.3 0 27 40 23 596.3 0 23 41 12 596.3 0 12 42 34 596.3 0 34 43 31 596.3 0 31 44 26 596.3 0 26 45 29 596.3 0 29 46 26 596.3 0 26 47 33 596.3 0 33 48 29 596.3 0 29 49 32 596.3 0 32 50 28 596.3 0 28 51 17 596.3 0 17 52 22 596.3 0 22 53 25 596.3 0 25 54 19 596.3 0 19 55 22 596.3 0 22 56 22 596.3 0 22 57 29 596.3 0 29 58 20 596.3 0 20 59 17 596.3 0 17 60 24 596.3 0 24 61 30 596.3 0 30 62 15 596.3 0 15 63 46 596.3 0 46 64 65 596.3 0 65 65 26 596.3 0 26 66 58 596.3 0 58 67 92 596.3 0 92 68 29 596.3 0 29 69 27 596.3 0 27 70 24 596.3 0 24 71 33 596.3 0 33 72 25 596.3 0 25 73 55 596.3 0 55 74 57 596.3 0 57 75 51 596.3 0 51 76 30 596.3 0 30 77 55 596.3 0 55 78 35 596.3 0 35 79 47 596.3 0 47 80 26 596.3 0 26 81 19 596.3 0 19 82 37 596.3 0 37 83 24 596.3 0 24 84 35 596.3 0 35 85 33 596.3 0 33 86 35 596.3 0 35 87 57 596.3 0 57 88 46 596.3 0 46 89 51 596.3 0 51 90 76 596.3 0 76 91 61 596.3 0 61 92 50 596.3 0 50 93 63 596.3 0 63 94 74 596.3 0 74 95 100 596.3 0 100 96 47 596.3 0 47 97 42 596.3 0 42 98 31 596.3 0 31 99 83 596.3 0 83 100 36 596.3 0 36 101 26 596.3 0 26 102 29 596.3 0 29 103 24 596.3 0 24 104 28 596.3 0 28 105 19 596.3 0 19 106 21 596.3 0 21 107 28 596.3 0 28 108 38 596.3 0 38 109 28 596.3 0 28 110 35 596.3 0 35 111 31 596.3 0 31 112 25 596.3 0 25 113 20 596.3 0 20 114 26 596.3 0 26 115 20 596.3 0 20 116 34 596.3 0 34 117 30 596.3 0 30 118 43 596.3 0 43 119 45 596.3 0 45 120 50 596.3 0 50 121 34 596.3 0 34 122 50 596.3 0 50 123 34 596.3 0 34 124 44 596.3 0 44 125 93 596.3 0 93 126 160 596.3 0 160 127 557 596.3 0 557 128 71 596.3 0 71 129 83 596.3 0 83 130 87 596.3 0 87 131 458 596.3 0 458 132 915 596.3 0 915 133 533 596.3 0 533 134 39 596.3 0 39 135 40 596.3 0 40 136 31 596.3 0 31 137 36 596.3 0 36 138 40 596.3 0 40 139 47 596.3 0 47 140 29 596.3 0 29 141 51 596.3 0 51 142 43 596.3 0 43 143 31 596.3 0 31 144 48 596.3 0 48 145 45 596.3 0 45 146 42 596.3 0 42 147 66 596.3 0 66 148 68 596.3 0 68 149 44 596.3 0 44 150 54 596.3 0 54 151 33 596.3 0 33 152 47 596.3 0 47 153 67 596.3 0 67 154 108 596.3 0 108 155 99 596.3 0 99 156 66 596.3 0 66 157 45 596.3 0 45 158 32 596.3 0 32 159 20 596.3 0 20 160 28 596.3 0 28 161 25 596.3 0 25 162 33 596.3 0 33 163 34 596.3 0 34 164 34 596.3 0 34 165 43 596.3 0 43 166 85 596.3 0 85 167 45 596.3 0 45 168 45 596.3 0 45 169 45 596.3 0 45 170 33 596.3 0 33 171 57 596.3 0 57 172 60 596.3 0 60 173 194 596.3 0 194 174 262 596.3 0 262 175 323 596.3 0 323 176 242 596.3 0 242 177 76 596.3 0 76 178 83 596.3 0 83 179 197 596.3 0 197 180 514 596.3 0 514 181 614 596.3 0 614 182 270 596.3 0 270 183 107 596.3 0 107 184 98 596.3 0 98 185 612 596.3 0 612 186 215 596.3 0 215 187 448 596.3 0 448 188 130 596.3 0 130 189 115 596.3 0 115 190 108 596.3 0 108 191 140 596.3 0 140 192 365 596.3 0 365 193 2286 596.3 0 2286 194 1784 596.3 0 1784 195 2317 596.3 0 2317 196 233 596.3 0 233 197 102 596.3 0 102 198 108 596.3 0 108 199 388 596.3 0 388 200 996 596.3 0 996 201 870 596.3 0 870 202 414 596.3 0 414 203 111 596.3 0 111 204 63 596.3 0 63 205 111 596.3 0 111 206 187 596.3 0 187 207 181 596.3 0 181 208 142 596.3 0 142 209 77 596.3 0 77 210 115 596.3 0 115 211 169 596.3 0 169 212 130 596.3 0 130 213 298 596.3 0 298 214 187 596.3 0 187 215 140 596.3 0 140 216 85 596.3 0 85 217 101 596.3 0 101 218 130 596.3 0 130 219 261 596.3 0 261 220 287 596.3 0 287 221 379 596.3 0 379 222 403 596.3 0 403 223 606 596.3 0 606 224 1026 596.3 0 1026 225 342 596.3 0 342 226 372 596.3 0 372 227 753 596.3 0 753 228 415 596.3 0 415 229 476 596.3 0 476 230 803 596.3 0 803 231 1162 596.3 0 1162 232 5937 596.3 0 5937 233 8238 596.3 0 8238 234 6992 596.3 0 6992 235 2254 596.3 0 2254 236 2032 596.3 0 2032 237 23697 596.3 0 23697 238 42330 596.3 0 42330 239 41753 596.3 0 41753 240 8124 596.3 0 8124 241 1592 596.3 0 1592 242 1344 596.3 0 1344 243 1981 596.3 0 1981 244 1275 596.3 0 1275 245 401 596.3 0 401 246 469 596.3 0 469 247 757 596.3 0 757 248 1201 596.3 0 1201 249 694 596.3 0 694 250 1589 596.3 0 1589 251 2095 596.3 0 2095 252 2496 596.3 0 2496 253 2719 596.3 0 2719 254 3287 596.3 0 3287 255 1475 596.3 0 1475 256 1925 596.3 0 1925 257 27585 596.3 0 27585 258 49506 596.3 0 49506 259 43811 596.3 0 43811 260 10137 596.3 0 10137 261 146 596.3 0 146 262 153 596.3 0 153 263 202 596.3 0 202 264 52 596.3 0 52 265 70 596.3 0 70 266 64 596.3 0 64 267 101 596.3 0 101 268 302 596.3 0 302 269 180 596.3 0 180 270 223 596.3 0 223 271 183 596.3 0 183 272 1094 596.3 0 1094 273 75 596.3 0 75 274 50 596.3 0 50 275 62 596.3 0 62 276 62 596.3 0 62 277 35 596.3 0 35 278 39 596.3 0 39 279 63 596.3 0 63 280 97 596.3 0 97 281 122 596.3 0 122 282 115 596.3 0 115 283 72 596.3 0 72 284 54 596.3 0 54 285 70 596.3 0 70 286 200 596.3 0 200 287 208 596.3 0 208 288 104 596.3 0 104 289 162 596.3 0 162 290 205 596.3 0 205 291 404 596.3 0 404 292 315 596.3 0 315 293 368 596.3 0 368 294 135 596.3 0 135 295 144 596.3 0 144 296 121 596.3 0 121 297 74 596.3 0 74 298 40 596.3 0 40 299 56 596.3 0 56 300 82 596.3 0 82 301 124 596.3 0 124 Finished in 2.06 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 48,295 Reads with adapters: 14,197 (29.4%) Reads that were too short: 108 (0.2%) Reads written (passing filters): 14,089 (29.2%) Total basepairs processed: 10,659,608 bp Total written (filtered): 3,871,969 bp (36.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 14197 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 11 0.0 3 0 0 7 4 16 124 0.0 3 0 105 8 11 17 13681 0.0 3 12786 737 125 33 18 67 0.0 3 3 45 5 14 19 51 0.0 3 1 2 27 21 20 52 0.0 3 2 1 1 48 21 3 0.0 3 1 1 0 1 25 1 0.0 3 0 0 0 1 31 4 0.0 3 0 0 1 3 33 2 0.0 3 0 0 1 1 35 2 0.0 3 0 0 0 2 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 43 7 0.0 3 0 0 0 7 44 2 0.0 3 0 0 0 2 46 2 0.0 3 0 0 0 2 47 31 0.0 3 0 0 2 29 48 13 0.0 3 0 0 0 13 50 5 0.0 3 0 0 0 5 51 4 0.0 3 0 0 0 4 52 6 0.0 3 0 0 0 6 54 1 0.0 3 0 0 0 1 60 16 0.0 3 0 0 1 15 61 2 0.0 3 0 0 0 2 62 2 0.0 3 0 0 0 2 63 3 0.0 3 0 0 0 3 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 74 7 0.0 3 0 0 1 6 76 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 85 2 0.0 3 0 0 1 1 87 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 93 3 0.0 3 0 0 0 3 94 5 0.0 3 0 0 1 4 95 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 100 2 0.0 3 0 0 0 2 101 2 0.0 3 0 0 0 2 102 3 0.0 3 0 0 0 3 103 1 0.0 3 1 104 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 3 0.0 3 0 0 1 2 109 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 130 2 0.0 3 0 0 0 2 132 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 153 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 3 0.0 3 0 0 0 3 161 2 0.0 3 0 0 0 2 162 3 0.0 3 0 0 0 3 163 3 0.0 3 0 0 0 3 164 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 1 175 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 195 5 0.0 3 0 0 0 5 212 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.49 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 14,089 Reads with adapters: 133 (0.9%) Reads written (passing filters): 14,089 (100.0%) Total basepairs processed: 3,871,969 bp Total written (filtered): 3,871,553 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 133 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 33.1% C: 32.3% G: 27.1% T: 7.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 121 220.1 0 121 4 9 55.0 0 9 5 2 13.8 0 2 7 1 0.9 0 1