This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 63.49 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 374,502 (15.3%) Reads that were too short: 328,878 (13.5%) Reads written (passing filters): 45,624 (1.9%) Total basepairs processed: 735,190,694 bp Total written (filtered): 10,552,860 bp (1.4%) === Adapter 1 === Sequence: GCGGTT; Type: regular 5'; Length: 6; Trimmed: 374502 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 239 596.3 0 239 7 361 596.3 0 361 8 11713 596.3 0 11713 9 13607 596.3 0 13607 10 51 596.3 0 51 11 49 596.3 0 49 12 35 596.3 0 35 13 46 596.3 0 46 14 37 596.3 0 37 15 34 596.3 0 34 16 34 596.3 0 34 17 46 596.3 0 46 18 37 596.3 0 37 19 26 596.3 0 26 20 76 596.3 0 76 21 28 596.3 0 28 22 60 596.3 0 60 23 32 596.3 0 32 24 44 596.3 0 44 25 33 596.3 0 33 26 51 596.3 0 51 27 42 596.3 0 42 28 28 596.3 0 28 29 30 596.3 0 30 30 20 596.3 0 20 31 32 596.3 0 32 32 27 596.3 0 27 33 13 596.3 0 13 34 34 596.3 0 34 35 43 596.3 0 43 36 25 596.3 0 25 37 36 596.3 0 36 38 38 596.3 0 38 39 44 596.3 0 44 40 34 596.3 0 34 41 34 596.3 0 34 42 27 596.3 0 27 43 24 596.3 0 24 44 21 596.3 0 21 45 27 596.3 0 27 46 36 596.3 0 36 47 36 596.3 0 36 48 27 596.3 0 27 49 46 596.3 0 46 50 29 596.3 0 29 51 40 596.3 0 40 52 30 596.3 0 30 53 35 596.3 0 35 54 31 596.3 0 31 55 29 596.3 0 29 56 34 596.3 0 34 57 29 596.3 0 29 58 23 596.3 0 23 59 26 596.3 0 26 60 27 596.3 0 27 61 33 596.3 0 33 62 27 596.3 0 27 63 65 596.3 0 65 64 79 596.3 0 79 65 35 596.3 0 35 66 68 596.3 0 68 67 98 596.3 0 98 68 27 596.3 0 27 69 43 596.3 0 43 70 28 596.3 0 28 71 42 596.3 0 42 72 33 596.3 0 33 73 44 596.3 0 44 74 33 596.3 0 33 75 40 596.3 0 40 76 55 596.3 0 55 77 54 596.3 0 54 78 36 596.3 0 36 79 34 596.3 0 34 80 36 596.3 0 36 81 34 596.3 0 34 82 35 596.3 0 35 83 26 596.3 0 26 84 39 596.3 0 39 85 46 596.3 0 46 86 57 596.3 0 57 87 33 596.3 0 33 88 44 596.3 0 44 89 65 596.3 0 65 90 71 596.3 0 71 91 73 596.3 0 73 92 63 596.3 0 63 93 47 596.3 0 47 94 37 596.3 0 37 95 43 596.3 0 43 96 28 596.3 0 28 97 37 596.3 0 37 98 24 596.3 0 24 99 41 596.3 0 41 100 35 596.3 0 35 101 25 596.3 0 25 102 55 596.3 0 55 103 34 596.3 0 34 104 49 596.3 0 49 105 42 596.3 0 42 106 41 596.3 0 41 107 43 596.3 0 43 108 22 596.3 0 22 109 42 596.3 0 42 110 28 596.3 0 28 111 38 596.3 0 38 112 41 596.3 0 41 113 40 596.3 0 40 114 34 596.3 0 34 115 37 596.3 0 37 116 60 596.3 0 60 117 48 596.3 0 48 118 49 596.3 0 49 119 40 596.3 0 40 120 55 596.3 0 55 121 38 596.3 0 38 122 43 596.3 0 43 123 43 596.3 0 43 124 54 596.3 0 54 125 59 596.3 0 59 126 131 596.3 0 131 127 208 596.3 0 208 128 65 596.3 0 65 129 74 596.3 0 74 130 57 596.3 0 57 131 447 596.3 0 447 132 871 596.3 0 871 133 543 596.3 0 543 134 49 596.3 0 49 135 53 596.3 0 53 136 44 596.3 0 44 137 40 596.3 0 40 138 48 596.3 0 48 139 50 596.3 0 50 140 49 596.3 0 49 141 65 596.3 0 65 142 48 596.3 0 48 143 65 596.3 0 65 144 44 596.3 0 44 145 42 596.3 0 42 146 69 596.3 0 69 147 54 596.3 0 54 148 69 596.3 0 69 149 48 596.3 0 48 150 76 596.3 0 76 151 53 596.3 0 53 152 66 596.3 0 66 153 68 596.3 0 68 154 93 596.3 0 93 155 78 596.3 0 78 156 81 596.3 0 81 157 70 596.3 0 70 158 61 596.3 0 61 159 64 596.3 0 64 160 61 596.3 0 61 161 75 596.3 0 75 162 53 596.3 0 53 163 65 596.3 0 65 164 66 596.3 0 66 165 74 596.3 0 74 166 86 596.3 0 86 167 83 596.3 0 83 168 84 596.3 0 84 169 86 596.3 0 86 170 74 596.3 0 74 171 77 596.3 0 77 172 82 596.3 0 82 173 244 596.3 0 244 174 194 596.3 0 194 175 239 596.3 0 239 176 167 596.3 0 167 177 77 596.3 0 77 178 86 596.3 0 86 179 122 596.3 0 122 180 207 596.3 0 207 181 231 596.3 0 231 182 160 596.3 0 160 183 110 596.3 0 110 184 104 596.3 0 104 185 658 596.3 0 658 186 262 596.3 0 262 187 388 596.3 0 388 188 114 596.3 0 114 189 122 596.3 0 122 190 122 596.3 0 122 191 154 596.3 0 154 192 265 596.3 0 265 193 1833 596.3 0 1833 194 1473 596.3 0 1473 195 1671 596.3 0 1671 196 142 596.3 0 142 197 91 596.3 0 91 198 93 596.3 0 93 199 213 596.3 0 213 200 310 596.3 0 310 201 319 596.3 0 319 202 200 596.3 0 200 203 89 596.3 0 89 204 90 596.3 0 90 205 113 596.3 0 113 206 143 596.3 0 143 207 165 596.3 0 165 208 124 596.3 0 124 209 98 596.3 0 98 210 114 596.3 0 114 211 185 596.3 0 185 212 133 596.3 0 133 213 321 596.3 0 321 214 189 596.3 0 189 215 191 596.3 0 191 216 153 596.3 0 153 217 147 596.3 0 147 218 176 596.3 0 176 219 308 596.3 0 308 220 255 596.3 0 255 221 303 596.3 0 303 222 386 596.3 0 386 223 630 596.3 0 630 224 1081 596.3 0 1081 225 391 596.3 0 391 226 401 596.3 0 401 227 804 596.3 0 804 228 416 596.3 0 416 229 481 596.3 0 481 230 761 596.3 0 761 231 1084 596.3 0 1084 232 6653 596.3 0 6653 233 9429 596.3 0 9429 234 8313 596.3 0 8313 235 2376 596.3 0 2376 236 1654 596.3 0 1654 237 23019 596.3 0 23019 238 44602 596.3 0 44602 239 43365 596.3 0 43365 240 8436 596.3 0 8436 241 1537 596.3 0 1537 242 1533 596.3 0 1533 243 2481 596.3 0 2481 244 1389 596.3 0 1389 245 563 596.3 0 563 246 517 596.3 0 517 247 854 596.3 0 854 248 1662 596.3 0 1662 249 758 596.3 0 758 250 1976 596.3 0 1976 251 2976 596.3 0 2976 252 2646 596.3 0 2646 253 2750 596.3 0 2750 254 4002 596.3 0 4002 255 1415 596.3 0 1415 256 1714 596.3 0 1714 257 24338 596.3 0 24338 258 52882 596.3 0 52882 259 44136 596.3 0 44136 260 13576 596.3 0 13576 261 115 596.3 0 115 262 101 596.3 0 101 263 98 596.3 0 98 264 84 596.3 0 84 265 87 596.3 0 87 266 78 596.3 0 78 267 100 596.3 0 100 268 277 596.3 0 277 269 129 596.3 0 129 270 222 596.3 0 222 271 154 596.3 0 154 272 1190 596.3 0 1190 273 78 596.3 0 78 274 66 596.3 0 66 275 77 596.3 0 77 276 97 596.3 0 97 277 55 596.3 0 55 278 70 596.3 0 70 279 97 596.3 0 97 280 107 596.3 0 107 281 95 596.3 0 95 282 107 596.3 0 107 283 90 596.3 0 90 284 72 596.3 0 72 285 95 596.3 0 95 286 118 596.3 0 118 287 126 596.3 0 126 288 112 596.3 0 112 289 132 596.3 0 132 290 225 596.3 0 225 291 625 596.3 0 625 292 672 596.3 0 672 293 620 596.3 0 620 294 291 596.3 0 291 295 134 596.3 0 134 296 132 596.3 0 132 297 108 596.3 0 108 298 74 596.3 0 74 299 69 596.3 0 69 300 95 596.3 0 95 301 120 596.3 0 120 Finished in 2.17 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 45,624 Reads with adapters: 14,476 (31.7%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 14,464 (31.7%) Total basepairs processed: 10,552,860 bp Total written (filtered): 3,928,384 bp (37.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 14476 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 10 0.0 3 0 0 10 19 337 0.0 3 0 330 6 1 20 14005 0.0 4 13692 300 13 21 29 0.0 4 2 27 22 9 0.0 4 4 1 4 23 7 0.0 4 0 0 0 5 2 24 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 118 1 0.0 4 1 127 63 0.0 4 0 0 0 0 63 158 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.53 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 14,464 Reads with adapters: 2,372 (16.4%) Reads written (passing filters): 14,464 (100.0%) Total basepairs processed: 3,928,384 bp Total written (filtered): 3,902,407 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2372 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.3% C: 3.0% G: 94.1% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3 226.0 0 3 4 3 56.5 0 3 6 2 3.5 0 2 9 1144 0.1 0 37 1107 10 1087 0.0 1 36 1051 11 108 0.0 1 0 108 12 7 0.0 1 0 7 138 5 0.0 1 4 1 139 1 0.0 1 1 223 11 0.0 1 10 1 224 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.