This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.90 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 171,454 (7.0%) Reads that were too short: 7,557 (0.3%) Reads written (passing filters): 163,897 (6.7%) Total basepairs processed: 735,190,694 bp Total written (filtered): 45,863,901 bp (6.2%) === Adapter 1 === Sequence: GTAGTG; Type: regular 5'; Length: 6; Trimmed: 171454 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1697 596.3 0 1697 7 76252 596.3 0 76252 8 1086 596.3 0 1086 9 64961 596.3 0 64961 10 46 596.3 0 46 11 18 596.3 0 18 12 8 596.3 0 8 13 16 596.3 0 16 14 14 596.3 0 14 15 11 596.3 0 11 16 17 596.3 0 17 17 15 596.3 0 15 18 15 596.3 0 15 19 10 596.3 0 10 20 7 596.3 0 7 21 14 596.3 0 14 22 27 596.3 0 27 23 15 596.3 0 15 24 12 596.3 0 12 25 15 596.3 0 15 26 49 596.3 0 49 27 53 596.3 0 53 28 92 596.3 0 92 29 66 596.3 0 66 30 60 596.3 0 60 31 45 596.3 0 45 32 19 596.3 0 19 33 12 596.3 0 12 34 15 596.3 0 15 35 14 596.3 0 14 36 14 596.3 0 14 37 19 596.3 0 19 38 12 596.3 0 12 39 29 596.3 0 29 40 17 596.3 0 17 41 8 596.3 0 8 42 32 596.3 0 32 43 24 596.3 0 24 44 22 596.3 0 22 45 10 596.3 0 10 46 18 596.3 0 18 47 14 596.3 0 14 48 13 596.3 0 13 49 25 596.3 0 25 50 12 596.3 0 12 51 25 596.3 0 25 52 36 596.3 0 36 53 27 596.3 0 27 54 28 596.3 0 28 55 19 596.3 0 19 56 20 596.3 0 20 57 35 596.3 0 35 58 28 596.3 0 28 59 31 596.3 0 31 60 24 596.3 0 24 61 29 596.3 0 29 62 27 596.3 0 27 63 35 596.3 0 35 64 34 596.3 0 34 65 34 596.3 0 34 66 40 596.3 0 40 67 65 596.3 0 65 68 121 596.3 0 121 69 1262 596.3 0 1262 70 569 596.3 0 569 71 3111 596.3 0 3111 72 38 596.3 0 38 73 38 596.3 0 38 74 24 596.3 0 24 75 35 596.3 0 35 76 31 596.3 0 31 77 106 596.3 0 106 78 31 596.3 0 31 79 24 596.3 0 24 80 16 596.3 0 16 81 26 596.3 0 26 82 27 596.3 0 27 83 25 596.3 0 25 84 18 596.3 0 18 85 24 596.3 0 24 86 30 596.3 0 30 87 20 596.3 0 20 88 24 596.3 0 24 89 19 596.3 0 19 90 16 596.3 0 16 91 23 596.3 0 23 92 25 596.3 0 25 93 13 596.3 0 13 94 28 596.3 0 28 95 22 596.3 0 22 96 28 596.3 0 28 97 36 596.3 0 36 98 33 596.3 0 33 99 34 596.3 0 34 100 27 596.3 0 27 101 28 596.3 0 28 102 26 596.3 0 26 103 13 596.3 0 13 104 11 596.3 0 11 105 14 596.3 0 14 106 20 596.3 0 20 107 21 596.3 0 21 108 19 596.3 0 19 109 18 596.3 0 18 110 28 596.3 0 28 111 30 596.3 0 30 112 32 596.3 0 32 113 34 596.3 0 34 114 59 596.3 0 59 115 191 596.3 0 191 116 44 596.3 0 44 117 28 596.3 0 28 118 18 596.3 0 18 119 14 596.3 0 14 120 11 596.3 0 11 121 22 596.3 0 22 122 20 596.3 0 20 123 23 596.3 0 23 124 29 596.3 0 29 125 24 596.3 0 24 126 19 596.3 0 19 127 42 596.3 0 42 128 53 596.3 0 53 129 43 596.3 0 43 130 44 596.3 0 44 131 28 596.3 0 28 132 27 596.3 0 27 133 125 596.3 0 125 134 57 596.3 0 57 135 69 596.3 0 69 136 64 596.3 0 64 137 72 596.3 0 72 138 454 596.3 0 454 139 853 596.3 0 853 140 308 596.3 0 308 141 255 596.3 0 255 142 400 596.3 0 400 143 295 596.3 0 295 144 277 596.3 0 277 145 667 596.3 0 667 146 68 596.3 0 68 147 84 596.3 0 84 148 735 596.3 0 735 149 1825 596.3 0 1825 150 1234 596.3 0 1234 151 2190 596.3 0 2190 152 62 596.3 0 62 153 104 596.3 0 104 154 17 596.3 0 17 155 28 596.3 0 28 156 26 596.3 0 26 157 32 596.3 0 32 158 14 596.3 0 14 159 26 596.3 0 26 160 11 596.3 0 11 161 20 596.3 0 20 162 28 596.3 0 28 163 16 596.3 0 16 164 17 596.3 0 17 165 15 596.3 0 15 166 17 596.3 0 17 167 17 596.3 0 17 168 20 596.3 0 20 169 20 596.3 0 20 170 11 596.3 0 11 171 13 596.3 0 13 172 16 596.3 0 16 173 20 596.3 0 20 174 21 596.3 0 21 175 27 596.3 0 27 176 26 596.3 0 26 177 13 596.3 0 13 178 17 596.3 0 17 179 20 596.3 0 20 180 13 596.3 0 13 181 14 596.3 0 14 182 15 596.3 0 15 183 16 596.3 0 16 184 43 596.3 0 43 185 52 596.3 0 52 186 36 596.3 0 36 187 21 596.3 0 21 188 28 596.3 0 28 189 47 596.3 0 47 190 73 596.3 0 73 191 120 596.3 0 120 192 64 596.3 0 64 193 38 596.3 0 38 194 24 596.3 0 24 195 26 596.3 0 26 196 24 596.3 0 24 197 21 596.3 0 21 198 16 596.3 0 16 199 31 596.3 0 31 200 22 596.3 0 22 201 23 596.3 0 23 202 25 596.3 0 25 203 20 596.3 0 20 204 22 596.3 0 22 205 23 596.3 0 23 206 26 596.3 0 26 207 21 596.3 0 21 208 17 596.3 0 17 209 62 596.3 0 62 210 74 596.3 0 74 211 42 596.3 0 42 212 27 596.3 0 27 213 19 596.3 0 19 214 28 596.3 0 28 215 46 596.3 0 46 216 20 596.3 0 20 217 17 596.3 0 17 218 8 596.3 0 8 219 23 596.3 0 23 220 31 596.3 0 31 221 18 596.3 0 18 222 9 596.3 0 9 223 25 596.3 0 25 224 18 596.3 0 18 225 10 596.3 0 10 226 16 596.3 0 16 227 23 596.3 0 23 228 12 596.3 0 12 229 29 596.3 0 29 230 19 596.3 0 19 231 20 596.3 0 20 232 25 596.3 0 25 233 45 596.3 0 45 234 67 596.3 0 67 235 106 596.3 0 106 236 79 596.3 0 79 237 36 596.3 0 36 238 38 596.3 0 38 239 37 596.3 0 37 240 40 596.3 0 40 241 34 596.3 0 34 242 31 596.3 0 31 243 52 596.3 0 52 244 40 596.3 0 40 245 40 596.3 0 40 246 31 596.3 0 31 247 49 596.3 0 49 248 74 596.3 0 74 249 170 596.3 0 170 250 102 596.3 0 102 251 318 596.3 0 318 252 59 596.3 0 59 253 309 596.3 0 309 254 703 596.3 0 703 255 654 596.3 0 654 256 44 596.3 0 44 257 29 596.3 0 29 258 51 596.3 0 51 259 34 596.3 0 34 260 30 596.3 0 30 261 22 596.3 0 22 262 36 596.3 0 36 263 28 596.3 0 28 264 32 596.3 0 32 265 26 596.3 0 26 266 49 596.3 0 49 267 31 596.3 0 31 268 94 596.3 0 94 269 101 596.3 0 101 270 63 596.3 0 63 271 26 596.3 0 26 272 43 596.3 0 43 273 111 596.3 0 111 274 168 596.3 0 168 275 125 596.3 0 125 276 44 596.3 0 44 277 31 596.3 0 31 278 62 596.3 0 62 279 41 596.3 0 41 280 50 596.3 0 50 281 64 596.3 0 64 282 55 596.3 0 55 283 47 596.3 0 47 284 38 596.3 0 38 285 77 596.3 0 77 286 75 596.3 0 75 287 100 596.3 0 100 288 88 596.3 0 88 289 120 596.3 0 120 290 59 596.3 0 59 291 61 596.3 0 61 292 109 596.3 0 109 293 105 596.3 0 105 294 99 596.3 0 99 295 139 596.3 0 139 296 92 596.3 0 92 297 143 596.3 0 143 298 123 596.3 0 123 299 161 596.3 0 161 300 286 596.3 0 286 301 256 596.3 0 256 Finished in 8.19 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 163,897 Reads with adapters: 78,039 (47.6%) Reads that were too short: 47 (0.0%) Reads written (passing filters): 77,992 (47.6%) Total basepairs processed: 45,863,901 bp Total written (filtered): 21,276,464 bp (46.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 78039 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 17 0.0 3 0 0 15 2 19 894 0.0 3 0 878 14 1 1 20 75968 0.0 4 74544 1360 57 6 1 21 156 0.0 4 30 126 22 15 0.0 4 3 0 9 1 2 23 62 0.0 4 2 1 1 51 7 24 5 0.0 4 1 0 0 0 4 25 1 0.0 4 1 27 1 0.0 4 1 28 1 0.0 4 1 30 1 0.0 4 1 41 1 0.0 4 1 45 1 0.0 4 1 76 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 97 3 0.0 4 0 0 0 0 3 101 1 0.0 4 0 1 103 1 0.0 4 0 0 0 0 1 108 6 0.0 4 0 0 0 0 6 114 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 126 15 0.0 4 0 0 0 0 15 127 832 0.0 4 0 0 0 0 832 128 5 0.0 4 0 0 0 0 5 130 3 0.0 4 0 0 0 0 3 131 1 0.0 4 0 0 0 0 1 136 2 0.0 4 0 0 0 0 2 146 1 0.0 4 1 160 2 0.0 4 1 0 1 202 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 1 215 1 0.0 4 0 0 0 0 1 220 2 0.0 4 0 0 0 0 2 221 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 227 3 0.0 4 0 0 0 0 3 228 13 0.0 4 0 0 0 0 13 229 7 0.0 4 0 0 0 0 7 230 4 0.0 4 0 0 0 0 4 234 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.68 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 77,992 Reads with adapters: 1,376 (1.8%) Reads written (passing filters): 77,992 (100.0%) Total basepairs processed: 21,276,464 bp Total written (filtered): 21,244,003 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1376 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 13.6% C: 6.1% G: 71.3% T: 9.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 218 1218.6 0 218 4 14 304.7 0 14 5 5 76.2 0 5 6 2 19.0 0 2 9 3 0.3 0 1 2 10 94 0.1 1 1 93 11 789 0.0 1 8 781 12 154 0.0 1 1 153 13 4 0.0 1 0 4 15 2 0.0 1 0 2 16 1 0.0 1 1 152 2 0.0 1 1 1 210 2 0.0 1 1 1 224 1 0.0 1 1 225 80 0.0 1 75 5 226 4 0.0 1 4 270 1 0.0 1 0 1