This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.48 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 85,431 (3.5%) Reads that were too short: 30,206 (1.2%) Reads written (passing filters): 55,225 (2.3%) Total basepairs processed: 735,190,694 bp Total written (filtered): 14,868,338 bp (2.0%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 85431 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 690 596.3 0 690 7 589 596.3 0 589 8 22986 596.3 0 22986 9 17971 596.3 0 17971 10 18 596.3 0 18 11 2 596.3 0 2 12 3 596.3 0 3 13 2 596.3 0 2 14 4 596.3 0 4 15 1 596.3 0 1 16 2 596.3 0 2 17 5 596.3 0 5 18 4 596.3 0 4 19 7 596.3 0 7 20 2 596.3 0 2 21 1 596.3 0 1 22 2 596.3 0 2 23 5 596.3 0 5 24 5 596.3 0 5 25 6 596.3 0 6 26 10 596.3 0 10 27 53 596.3 0 53 28 19 596.3 0 19 29 10 596.3 0 10 30 4 596.3 0 4 31 4 596.3 0 4 32 7 596.3 0 7 33 23 596.3 0 23 34 5 596.3 0 5 35 4 596.3 0 4 36 1 596.3 0 1 37 2 596.3 0 2 38 3 596.3 0 3 39 7 596.3 0 7 40 15 596.3 0 15 41 4 596.3 0 4 42 5 596.3 0 5 43 13 596.3 0 13 44 10 596.3 0 10 45 7 596.3 0 7 46 10 596.3 0 10 47 4 596.3 0 4 48 6 596.3 0 6 49 12 596.3 0 12 50 12 596.3 0 12 51 10 596.3 0 10 52 21 596.3 0 21 53 17 596.3 0 17 54 19 596.3 0 19 55 16 596.3 0 16 56 16 596.3 0 16 57 27 596.3 0 27 58 51 596.3 0 51 59 196 596.3 0 196 60 1425 596.3 0 1425 61 792 596.3 0 792 62 1663 596.3 0 1663 63 19 596.3 0 19 64 15 596.3 0 15 65 20 596.3 0 20 66 98 596.3 0 98 67 31 596.3 0 31 68 58 596.3 0 58 69 6 596.3 0 6 70 13 596.3 0 13 71 12 596.3 0 12 72 19 596.3 0 19 73 8 596.3 0 8 74 11 596.3 0 11 75 15 596.3 0 15 76 27 596.3 0 27 77 281 596.3 0 281 78 97 596.3 0 97 79 331 596.3 0 331 80 17 596.3 0 17 81 36 596.3 0 36 82 15 596.3 0 15 83 26 596.3 0 26 84 62 596.3 0 62 85 18 596.3 0 18 86 32 596.3 0 32 87 12 596.3 0 12 88 12 596.3 0 12 89 5 596.3 0 5 90 30 596.3 0 30 91 17 596.3 0 17 92 8 596.3 0 8 93 20 596.3 0 20 94 47 596.3 0 47 95 36 596.3 0 36 96 6 596.3 0 6 97 20 596.3 0 20 98 17 596.3 0 17 99 23 596.3 0 23 100 15 596.3 0 15 101 10 596.3 0 10 102 18 596.3 0 18 103 18 596.3 0 18 104 34 596.3 0 34 105 15 596.3 0 15 106 16 596.3 0 16 107 18 596.3 0 18 108 18 596.3 0 18 109 18 596.3 0 18 110 21 596.3 0 21 111 83 596.3 0 83 112 191 596.3 0 191 113 254 596.3 0 254 114 790 596.3 0 790 115 812 596.3 0 812 116 533 596.3 0 533 117 124 596.3 0 124 118 16 596.3 0 16 119 12 596.3 0 12 120 9 596.3 0 9 121 14 596.3 0 14 122 13 596.3 0 13 123 16 596.3 0 16 124 21 596.3 0 21 125 11 596.3 0 11 126 13 596.3 0 13 127 14 596.3 0 14 128 12 596.3 0 12 129 19 596.3 0 19 130 25 596.3 0 25 131 49 596.3 0 49 132 35 596.3 0 35 133 34 596.3 0 34 134 17 596.3 0 17 135 26 596.3 0 26 136 21 596.3 0 21 137 50 596.3 0 50 138 28 596.3 0 28 139 35 596.3 0 35 140 24 596.3 0 24 141 17 596.3 0 17 142 21 596.3 0 21 143 44 596.3 0 44 144 44 596.3 0 44 145 41 596.3 0 41 146 36 596.3 0 36 147 33 596.3 0 33 148 20 596.3 0 20 149 55 596.3 0 55 150 30 596.3 0 30 151 15 596.3 0 15 152 21 596.3 0 21 153 18 596.3 0 18 154 21 596.3 0 21 155 17 596.3 0 17 156 31 596.3 0 31 157 20 596.3 0 20 158 11 596.3 0 11 159 21 596.3 0 21 160 18 596.3 0 18 161 22 596.3 0 22 162 21 596.3 0 21 163 47 596.3 0 47 164 119 596.3 0 119 165 151 596.3 0 151 166 99 596.3 0 99 167 82 596.3 0 82 168 22 596.3 0 22 169 8 596.3 0 8 170 17 596.3 0 17 171 32 596.3 0 32 172 14 596.3 0 14 173 15 596.3 0 15 174 17 596.3 0 17 175 10 596.3 0 10 176 26 596.3 0 26 177 17 596.3 0 17 178 33 596.3 0 33 179 134 596.3 0 134 180 83 596.3 0 83 181 223 596.3 0 223 182 32 596.3 0 32 183 27 596.3 0 27 184 76 596.3 0 76 185 70 596.3 0 70 186 73 596.3 0 73 187 51 596.3 0 51 188 26 596.3 0 26 189 66 596.3 0 66 190 147 596.3 0 147 191 377 596.3 0 377 192 247 596.3 0 247 193 101 596.3 0 101 194 18 596.3 0 18 195 29 596.3 0 29 196 43 596.3 0 43 197 74 596.3 0 74 198 68 596.3 0 68 199 53 596.3 0 53 200 46 596.3 0 46 201 45 596.3 0 45 202 85 596.3 0 85 203 259 596.3 0 259 204 72 596.3 0 72 205 265 596.3 0 265 206 35 596.3 0 35 207 85 596.3 0 85 208 142 596.3 0 142 209 139 596.3 0 139 210 148 596.3 0 148 211 65 596.3 0 65 212 109 596.3 0 109 213 41 596.3 0 41 214 57 596.3 0 57 215 41 596.3 0 41 216 116 596.3 0 116 217 108 596.3 0 108 218 91 596.3 0 91 219 70 596.3 0 70 220 39 596.3 0 39 221 37 596.3 0 37 222 37 596.3 0 37 223 33 596.3 0 33 224 87 596.3 0 87 225 106 596.3 0 106 226 137 596.3 0 137 227 66 596.3 0 66 228 73 596.3 0 73 229 39 596.3 0 39 230 53 596.3 0 53 231 104 596.3 0 104 232 163 596.3 0 163 233 114 596.3 0 114 234 119 596.3 0 119 235 188 596.3 0 188 236 461 596.3 0 461 237 497 596.3 0 497 238 543 596.3 0 543 239 369 596.3 0 369 240 115 596.3 0 115 241 121 596.3 0 121 242 151 596.3 0 151 243 176 596.3 0 176 244 334 596.3 0 334 245 4268 596.3 0 4268 246 5031 596.3 0 5031 247 6491 596.3 0 6491 248 80 596.3 0 80 249 44 596.3 0 44 250 61 596.3 0 61 251 53 596.3 0 53 252 67 596.3 0 67 253 95 596.3 0 95 254 119 596.3 0 119 255 85 596.3 0 85 256 76 596.3 0 76 257 48 596.3 0 48 258 59 596.3 0 59 259 93 596.3 0 93 260 346 596.3 0 346 261 192 596.3 0 192 262 338 596.3 0 338 263 123 596.3 0 123 264 169 596.3 0 169 265 815 596.3 0 815 266 949 596.3 0 949 267 476 596.3 0 476 268 61 596.3 0 61 269 48 596.3 0 48 270 162 596.3 0 162 271 291 596.3 0 291 272 665 596.3 0 665 273 198 596.3 0 198 274 34 596.3 0 34 275 35 596.3 0 35 276 29 596.3 0 29 277 109 596.3 0 109 278 122 596.3 0 122 279 198 596.3 0 198 280 167 596.3 0 167 281 94 596.3 0 94 282 45 596.3 0 45 283 67 596.3 0 67 284 109 596.3 0 109 285 85 596.3 0 85 286 91 596.3 0 91 287 79 596.3 0 79 288 67 596.3 0 67 289 99 596.3 0 99 290 86 596.3 0 86 291 38 596.3 0 38 292 47 596.3 0 47 293 77 596.3 0 77 294 101 596.3 0 101 295 97 596.3 0 97 296 84 596.3 0 84 297 76 596.3 0 76 298 225 596.3 0 225 299 135 596.3 0 135 300 122 596.3 0 122 301 95 596.3 0 95 Finished in 2.56 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 55,225 Reads with adapters: 23,111 (41.8%) Reads that were too short: 85 (0.2%) Reads written (passing filters): 23,026 (41.7%) Total basepairs processed: 14,868,338 bp Total written (filtered): 6,336,341 bp (42.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 23111 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 14 0.0 3 0 0 8 6 16 266 0.0 3 0 229 19 18 17 22436 0.0 3 20978 1208 205 45 18 58 0.0 3 3 41 8 6 19 69 0.0 3 0 0 44 25 20 58 0.0 3 0 2 0 56 26 5 0.0 3 0 0 0 5 28 7 0.0 3 0 0 0 7 31 4 0.0 3 0 0 0 4 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 56 4 0.0 3 0 0 0 4 57 2 0.0 3 0 0 0 2 58 3 0.0 3 0 0 0 3 60 2 0.0 3 0 0 0 2 61 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 76 2 0.0 3 0 0 0 2 77 16 0.0 3 0 0 0 16 78 35 0.0 3 0 0 0 35 79 3 0.0 3 0 0 0 3 80 4 0.0 3 0 0 0 4 81 6 0.0 3 0 0 0 6 82 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 89 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 105 3 0.0 3 0 0 0 3 106 2 0.0 3 0 0 0 2 108 2 0.0 3 0 0 0 2 109 3 0.0 3 0 0 1 2 118 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 137 3 0.0 3 0 0 0 3 138 2 0.0 3 0 0 0 2 145 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 0 1 148 4 0.0 3 0 0 0 4 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 154 2 0.0 3 0 0 0 2 157 1 0.0 3 0 0 1 158 3 0.0 3 0 0 0 3 163 1 0.0 3 0 0 0 1 164 5 0.0 3 0 0 0 5 165 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 168 1 0.0 3 0 0 0 1 170 2 0.0 3 1 0 0 1 177 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 184 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 1 189 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 229 3 0.0 3 0 0 0 3 230 2 0.0 3 0 0 1 1 231 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 285 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.79 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 23,026 Reads with adapters: 121 (0.5%) Reads written (passing filters): 23,026 (100.0%) Total basepairs processed: 6,336,341 bp Total written (filtered): 6,335,959 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 121 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 55.4% C: 19.8% G: 19.0% T: 5.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 113 359.8 0 113 4 4 89.9 0 4 5 3 22.5 0 3 12 1 0.0 1 0 1