This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.25 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 77,008 (3.2%) Reads that were too short: 25,987 (1.1%) Reads written (passing filters): 51,021 (2.1%) Total basepairs processed: 735,190,694 bp Total written (filtered): 14,147,822 bp (1.9%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 77008 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 726 596.3 0 726 7 491 596.3 0 491 8 20378 596.3 0 20378 9 22031 596.3 0 22031 10 16 596.3 0 16 11 2 596.3 0 2 12 3 596.3 0 3 13 1 596.3 0 1 14 3 596.3 0 3 16 2 596.3 0 2 17 2 596.3 0 2 18 5 596.3 0 5 19 2 596.3 0 2 20 2 596.3 0 2 21 2 596.3 0 2 22 1 596.3 0 1 23 3 596.3 0 3 24 2 596.3 0 2 25 5 596.3 0 5 26 6 596.3 0 6 27 2 596.3 0 2 28 2 596.3 0 2 29 2 596.3 0 2 30 3 596.3 0 3 31 2 596.3 0 2 32 2 596.3 0 2 33 10 596.3 0 10 34 2 596.3 0 2 35 1 596.3 0 1 36 3 596.3 0 3 37 3 596.3 0 3 38 2 596.3 0 2 39 7 596.3 0 7 40 9 596.3 0 9 41 9 596.3 0 9 42 8 596.3 0 8 43 16 596.3 0 16 44 4 596.3 0 4 45 3 596.3 0 3 46 4 596.3 0 4 47 4 596.3 0 4 48 9 596.3 0 9 49 13 596.3 0 13 50 6 596.3 0 6 51 6 596.3 0 6 52 3 596.3 0 3 53 5 596.3 0 5 54 6 596.3 0 6 55 11 596.3 0 11 56 13 596.3 0 13 57 27 596.3 0 27 58 51 596.3 0 51 59 108 596.3 0 108 60 717 596.3 0 717 61 342 596.3 0 342 62 985 596.3 0 985 63 12 596.3 0 12 64 7 596.3 0 7 65 5 596.3 0 5 66 58 596.3 0 58 67 45 596.3 0 45 68 41 596.3 0 41 69 4 596.3 0 4 70 6 596.3 0 6 71 3 596.3 0 3 72 10 596.3 0 10 73 8 596.3 0 8 74 5 596.3 0 5 75 8 596.3 0 8 76 10 596.3 0 10 77 131 596.3 0 131 78 62 596.3 0 62 79 253 596.3 0 253 80 12 596.3 0 12 81 26 596.3 0 26 82 12 596.3 0 12 83 19 596.3 0 19 84 12 596.3 0 12 85 12 596.3 0 12 86 35 596.3 0 35 87 6 596.3 0 6 88 8 596.3 0 8 89 9 596.3 0 9 90 32 596.3 0 32 91 7 596.3 0 7 92 12 596.3 0 12 93 8 596.3 0 8 94 14 596.3 0 14 95 17 596.3 0 17 96 6 596.3 0 6 97 18 596.3 0 18 98 15 596.3 0 15 99 28 596.3 0 28 100 17 596.3 0 17 101 10 596.3 0 10 102 7 596.3 0 7 103 12 596.3 0 12 104 24 596.3 0 24 105 7 596.3 0 7 106 6 596.3 0 6 107 13 596.3 0 13 108 11 596.3 0 11 109 16 596.3 0 16 110 10 596.3 0 10 111 43 596.3 0 43 112 90 596.3 0 90 113 116 596.3 0 116 114 219 596.3 0 219 115 341 596.3 0 341 116 187 596.3 0 187 117 85 596.3 0 85 118 8 596.3 0 8 119 6 596.3 0 6 120 4 596.3 0 4 121 7 596.3 0 7 122 15 596.3 0 15 123 17 596.3 0 17 124 10 596.3 0 10 125 14 596.3 0 14 126 15 596.3 0 15 127 8 596.3 0 8 128 8 596.3 0 8 129 8 596.3 0 8 130 13 596.3 0 13 131 14 596.3 0 14 132 12 596.3 0 12 133 26 596.3 0 26 134 12 596.3 0 12 135 11 596.3 0 11 136 14 596.3 0 14 137 36 596.3 0 36 138 25 596.3 0 25 139 17 596.3 0 17 140 15 596.3 0 15 141 5 596.3 0 5 142 11 596.3 0 11 143 22 596.3 0 22 144 22 596.3 0 22 145 21 596.3 0 21 146 12 596.3 0 12 147 6 596.3 0 6 148 10 596.3 0 10 149 40 596.3 0 40 150 21 596.3 0 21 151 15 596.3 0 15 152 21 596.3 0 21 153 9 596.3 0 9 154 10 596.3 0 10 155 11 596.3 0 11 156 15 596.3 0 15 157 6 596.3 0 6 158 11 596.3 0 11 159 16 596.3 0 16 160 14 596.3 0 14 161 15 596.3 0 15 162 11 596.3 0 11 163 13 596.3 0 13 164 51 596.3 0 51 165 47 596.3 0 47 166 47 596.3 0 47 167 38 596.3 0 38 168 17 596.3 0 17 169 7 596.3 0 7 170 16 596.3 0 16 171 14 596.3 0 14 172 14 596.3 0 14 173 7 596.3 0 7 174 12 596.3 0 12 175 12 596.3 0 12 176 10 596.3 0 10 177 9 596.3 0 9 178 28 596.3 0 28 179 137 596.3 0 137 180 90 596.3 0 90 181 228 596.3 0 228 182 14 596.3 0 14 183 15 596.3 0 15 184 40 596.3 0 40 185 24 596.3 0 24 186 25 596.3 0 25 187 36 596.3 0 36 188 21 596.3 0 21 189 52 596.3 0 52 190 157 596.3 0 157 191 500 596.3 0 500 192 138 596.3 0 138 193 116 596.3 0 116 194 19 596.3 0 19 195 10 596.3 0 10 196 29 596.3 0 29 197 23 596.3 0 23 198 23 596.3 0 23 199 21 596.3 0 21 200 23 596.3 0 23 201 26 596.3 0 26 202 94 596.3 0 94 203 250 596.3 0 250 204 57 596.3 0 57 205 332 596.3 0 332 206 14 596.3 0 14 207 38 596.3 0 38 208 79 596.3 0 79 209 55 596.3 0 55 210 79 596.3 0 79 211 30 596.3 0 30 212 96 596.3 0 96 213 23 596.3 0 23 214 41 596.3 0 41 215 21 596.3 0 21 216 34 596.3 0 34 217 47 596.3 0 47 218 25 596.3 0 25 219 40 596.3 0 40 220 12 596.3 0 12 221 29 596.3 0 29 222 23 596.3 0 23 223 20 596.3 0 20 224 45 596.3 0 45 225 37 596.3 0 37 226 36 596.3 0 36 227 32 596.3 0 32 228 23 596.3 0 23 229 32 596.3 0 32 230 38 596.3 0 38 231 63 596.3 0 63 232 137 596.3 0 137 233 69 596.3 0 69 234 71 596.3 0 71 235 141 596.3 0 141 236 254 596.3 0 254 237 305 596.3 0 305 238 241 596.3 0 241 239 193 596.3 0 193 240 86 596.3 0 86 241 92 596.3 0 92 242 108 596.3 0 108 243 142 596.3 0 142 244 229 596.3 0 229 245 4411 596.3 0 4411 246 5428 596.3 0 5428 247 6749 596.3 0 6749 248 58 596.3 0 58 249 24 596.3 0 24 250 33 596.3 0 33 251 38 596.3 0 38 252 44 596.3 0 44 253 52 596.3 0 52 254 59 596.3 0 59 255 38 596.3 0 38 256 43 596.3 0 43 257 27 596.3 0 27 258 43 596.3 0 43 259 46 596.3 0 46 260 304 596.3 0 304 261 161 596.3 0 161 262 348 596.3 0 348 263 87 596.3 0 87 264 82 596.3 0 82 265 740 596.3 0 740 266 791 596.3 0 791 267 254 596.3 0 254 268 30 596.3 0 30 269 25 596.3 0 25 270 81 596.3 0 81 271 117 596.3 0 117 272 295 596.3 0 295 273 171 596.3 0 171 274 15 596.3 0 15 275 30 596.3 0 30 276 18 596.3 0 18 277 52 596.3 0 52 278 36 596.3 0 36 279 51 596.3 0 51 280 64 596.3 0 64 281 37 596.3 0 37 282 21 596.3 0 21 283 26 596.3 0 26 284 51 596.3 0 51 285 49 596.3 0 49 286 51 596.3 0 51 287 51 596.3 0 51 288 42 596.3 0 42 289 44 596.3 0 44 290 76 596.3 0 76 291 27 596.3 0 27 292 43 596.3 0 43 293 47 596.3 0 47 294 104 596.3 0 104 295 68 596.3 0 68 296 56 596.3 0 56 297 55 596.3 0 55 298 252 596.3 0 252 299 77 596.3 0 77 300 102 596.3 0 102 301 50 596.3 0 50 Finished in 2.75 s (54 us/read; 1.11 M reads/minute). === Summary === Total reads processed: 51,021 Reads with adapters: 24,580 (48.2%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 24,572 (48.2%) Total basepairs processed: 14,147,822 bp Total written (filtered): 6,559,745 bp (46.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 24580 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 2 0.0 3 0 0 0 2 18 16 0.0 3 0 0 16 19 368 0.0 3 0 361 7 20 22424 0.0 4 21942 463 16 2 1 21 73 0.0 4 13 52 6 2 22 481 0.0 4 466 8 6 1 23 16 0.0 4 0 1 0 13 2 24 2 0.0 4 0 0 0 1 1 25 1 0.0 4 0 0 0 1 26 1 0.0 4 0 0 0 0 1 27 1 0.0 4 0 0 1 29 1 0.0 4 0 0 0 0 1 112 2 0.0 4 0 0 0 0 2 125 2 0.0 4 0 0 0 0 2 126 19 0.0 4 0 0 0 0 19 127 1150 0.0 4 0 0 0 0 1150 128 9 0.0 4 0 0 0 0 9 129 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 249 1 0.0 4 0 0 0 0 1 253 1 0.0 4 0 0 1 260 1 0.0 4 0 0 0 0 1 282 2 0.0 4 0 0 0 0 2 283 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.85 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 24,572 Reads with adapters: 2,934 (11.9%) Reads written (passing filters): 24,572 (100.0%) Total basepairs processed: 6,559,745 bp Total written (filtered): 6,528,657 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2934 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.5% C: 3.1% G: 93.3% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 5 383.9 0 5 4 5 96.0 0 5 6 1 6.0 0 1 7 1 1.5 0 1 9 937 0.1 0 23 914 10 1732 0.0 1 71 1661 11 228 0.0 1 2 226 12 2 0.0 1 0 2 13 4 0.0 1 0 4 17 1 0.0 1 0 1 18 1 0.0 1 1 19 2 0.0 1 2 22 1 0.0 1 1 23 1 0.0 1 1 70 1 0.0 1 0 1 169 3 0.0 1 3 223 8 0.0 1 7 1 224 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.