This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.58 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 263,756 (10.7%) Reads that were too short: 88,702 (3.6%) Reads written (passing filters): 175,054 (7.1%) Total basepairs processed: 740,966,583 bp Total written (filtered): 48,396,279 bp (6.5%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 263756 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2263 601.0 0 2263 7 81013 601.0 0 81013 8 66017 601.0 0 66017 9 121 601.0 0 121 10 77 601.0 0 77 11 86 601.0 0 86 12 49 601.0 0 49 13 73 601.0 0 73 14 76 601.0 0 76 15 131 601.0 0 131 16 124 601.0 0 124 17 98 601.0 0 98 18 47 601.0 0 47 19 63 601.0 0 63 20 75 601.0 0 75 21 58 601.0 0 58 22 60 601.0 0 60 23 52 601.0 0 52 24 68 601.0 0 68 25 80 601.0 0 80 26 59 601.0 0 59 27 45 601.0 0 45 28 45 601.0 0 45 29 57 601.0 0 57 30 59 601.0 0 59 31 75 601.0 0 75 32 66 601.0 0 66 33 52 601.0 0 52 34 91 601.0 0 91 35 88 601.0 0 88 36 71 601.0 0 71 37 67 601.0 0 67 38 70 601.0 0 70 39 51 601.0 0 51 40 71 601.0 0 71 41 83 601.0 0 83 42 69 601.0 0 69 43 133 601.0 0 133 44 72 601.0 0 72 45 73 601.0 0 73 46 76 601.0 0 76 47 74 601.0 0 74 48 76 601.0 0 76 49 64 601.0 0 64 50 60 601.0 0 60 51 67 601.0 0 67 52 155 601.0 0 155 53 139 601.0 0 139 54 127 601.0 0 127 55 95 601.0 0 95 56 90 601.0 0 90 57 59 601.0 0 59 58 77 601.0 0 77 59 127 601.0 0 127 60 97 601.0 0 97 61 287 601.0 0 287 62 82 601.0 0 82 63 93 601.0 0 93 64 100 601.0 0 100 65 86 601.0 0 86 66 81 601.0 0 81 67 81 601.0 0 81 68 98 601.0 0 98 69 82 601.0 0 82 70 85 601.0 0 85 71 108 601.0 0 108 72 160 601.0 0 160 73 243 601.0 0 243 74 302 601.0 0 302 75 115 601.0 0 115 76 70 601.0 0 70 77 75 601.0 0 75 78 89 601.0 0 89 79 91 601.0 0 91 80 104 601.0 0 104 81 137 601.0 0 137 82 95 601.0 0 95 83 103 601.0 0 103 84 76 601.0 0 76 85 98 601.0 0 98 86 101 601.0 0 101 87 81 601.0 0 81 88 77 601.0 0 77 89 68 601.0 0 68 90 79 601.0 0 79 91 75 601.0 0 75 92 83 601.0 0 83 93 83 601.0 0 83 94 85 601.0 0 85 95 97 601.0 0 97 96 116 601.0 0 116 97 77 601.0 0 77 98 86 601.0 0 86 99 81 601.0 0 81 100 84 601.0 0 84 101 88 601.0 0 88 102 149 601.0 0 149 103 146 601.0 0 146 104 147 601.0 0 147 105 100 601.0 0 100 106 118 601.0 0 118 107 144 601.0 0 144 108 97 601.0 0 97 109 74 601.0 0 74 110 93 601.0 0 93 111 102 601.0 0 102 112 96 601.0 0 96 113 82 601.0 0 82 114 105 601.0 0 105 115 69 601.0 0 69 116 76 601.0 0 76 117 68 601.0 0 68 118 86 601.0 0 86 119 72 601.0 0 72 120 97 601.0 0 97 121 110 601.0 0 110 122 142 601.0 0 142 123 124 601.0 0 124 124 455 601.0 0 455 125 406 601.0 0 406 126 310 601.0 0 310 127 98 601.0 0 98 128 98 601.0 0 98 129 76 601.0 0 76 130 128 601.0 0 128 131 93 601.0 0 93 132 129 601.0 0 129 133 177 601.0 0 177 134 243 601.0 0 243 135 286 601.0 0 286 136 100 601.0 0 100 137 142 601.0 0 142 138 116 601.0 0 116 139 230 601.0 0 230 140 105 601.0 0 105 141 144 601.0 0 144 142 102 601.0 0 102 143 115 601.0 0 115 144 103 601.0 0 103 145 150 601.0 0 150 146 229 601.0 0 229 147 283 601.0 0 283 148 226 601.0 0 226 149 410 601.0 0 410 150 175 601.0 0 175 151 152 601.0 0 152 152 145 601.0 0 145 153 130 601.0 0 130 154 139 601.0 0 139 155 130 601.0 0 130 156 139 601.0 0 139 157 163 601.0 0 163 158 234 601.0 0 234 159 207 601.0 0 207 160 288 601.0 0 288 161 182 601.0 0 182 162 263 601.0 0 263 163 140 601.0 0 140 164 130 601.0 0 130 165 147 601.0 0 147 166 189 601.0 0 189 167 210 601.0 0 210 168 193 601.0 0 193 169 387 601.0 0 387 170 337 601.0 0 337 171 495 601.0 0 495 172 317 601.0 0 317 173 250 601.0 0 250 174 146 601.0 0 146 175 124 601.0 0 124 176 113 601.0 0 113 177 99 601.0 0 99 178 109 601.0 0 109 179 147 601.0 0 147 180 218 601.0 0 218 181 240 601.0 0 240 182 193 601.0 0 193 183 207 601.0 0 207 184 223 601.0 0 223 185 175 601.0 0 175 186 161 601.0 0 161 187 300 601.0 0 300 188 368 601.0 0 368 189 409 601.0 0 409 190 145 601.0 0 145 191 120 601.0 0 120 192 143 601.0 0 143 193 166 601.0 0 166 194 173 601.0 0 173 195 157 601.0 0 157 196 132 601.0 0 132 197 115 601.0 0 115 198 131 601.0 0 131 199 139 601.0 0 139 200 167 601.0 0 167 201 128 601.0 0 128 202 123 601.0 0 123 203 184 601.0 0 184 204 237 601.0 0 237 205 235 601.0 0 235 206 134 601.0 0 134 207 102 601.0 0 102 208 148 601.0 0 148 209 175 601.0 0 175 210 149 601.0 0 149 211 175 601.0 0 175 212 157 601.0 0 157 213 232 601.0 0 232 214 635 601.0 0 635 215 909 601.0 0 909 216 1671 601.0 0 1671 217 1714 601.0 0 1714 218 316 601.0 0 316 219 392 601.0 0 392 220 264 601.0 0 264 221 263 601.0 0 263 222 236 601.0 0 236 223 294 601.0 0 294 224 363 601.0 0 363 225 692 601.0 0 692 226 2529 601.0 0 2529 227 6227 601.0 0 6227 228 11242 601.0 0 11242 229 10274 601.0 0 10274 230 1572 601.0 0 1572 231 1116 601.0 0 1116 232 1965 601.0 0 1965 233 1280 601.0 0 1280 234 167 601.0 0 167 235 161 601.0 0 161 236 163 601.0 0 163 237 254 601.0 0 254 238 276 601.0 0 276 239 380 601.0 0 380 240 267 601.0 0 267 241 184 601.0 0 184 242 114 601.0 0 114 243 127 601.0 0 127 244 146 601.0 0 146 245 153 601.0 0 153 246 196 601.0 0 196 247 189 601.0 0 189 248 259 601.0 0 259 249 290 601.0 0 290 250 199 601.0 0 199 251 153 601.0 0 153 252 127 601.0 0 127 253 134 601.0 0 134 254 254 601.0 0 254 255 217 601.0 0 217 256 254 601.0 0 254 257 359 601.0 0 359 258 336 601.0 0 336 259 543 601.0 0 543 260 381 601.0 0 381 261 301 601.0 0 301 262 321 601.0 0 321 263 397 601.0 0 397 264 917 601.0 0 917 265 2592 601.0 0 2592 266 5096 601.0 0 5096 267 5686 601.0 0 5686 268 4008 601.0 0 4008 269 617 601.0 0 617 270 488 601.0 0 488 271 445 601.0 0 445 272 424 601.0 0 424 273 470 601.0 0 470 274 492 601.0 0 492 275 631 601.0 0 631 276 579 601.0 0 579 277 263 601.0 0 263 278 341 601.0 0 341 279 519 601.0 0 519 280 455 601.0 0 455 281 419 601.0 0 419 282 223 601.0 0 223 283 214 601.0 0 214 284 227 601.0 0 227 285 341 601.0 0 341 286 524 601.0 0 524 287 576 601.0 0 576 288 344 601.0 0 344 289 263 601.0 0 263 290 358 601.0 0 358 291 2724 601.0 0 2724 292 1719 601.0 0 1719 293 1685 601.0 0 1685 294 236 601.0 0 236 295 182 601.0 0 182 296 117 601.0 0 117 297 141 601.0 0 141 298 133 601.0 0 133 299 172 601.0 0 172 300 223 601.0 0 223 301 551 601.0 0 551 Finished in 8.08 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 175,054 Reads with adapters: 80,404 (45.9%) Reads that were too short: 254 (0.1%) Reads written (passing filters): 80,150 (45.8%) Total basepairs processed: 48,396,279 bp Total written (filtered): 22,174,178 bp (45.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 80404 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 16 0.0 2 0 0 0 16 15 55 0.0 3 0 0 26 29 16 692 0.0 3 0 538 95 59 17 78460 0.0 3 71902 5276 1048 234 18 190 0.0 3 21 140 14 15 19 301 0.0 3 0 0 179 122 20 254 0.0 3 0 0 0 254 21 1 0.0 3 0 0 0 1 22 4 0.0 3 1 0 0 3 23 11 0.0 3 0 0 0 11 24 2 0.0 3 0 0 0 2 25 4 0.0 3 0 0 0 4 26 3 0.0 3 0 0 0 3 28 3 0.0 3 0 0 1 2 29 2 0.0 3 2 30 1 0.0 3 0 0 0 1 32 6 0.0 3 0 0 0 6 33 14 0.0 3 0 0 11 3 35 4 0.0 3 0 0 0 4 37 1 0.0 3 1 39 2 0.0 3 1 0 0 1 40 3 0.0 3 0 0 0 3 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 1 1 48 2 0.0 3 0 0 0 2 49 3 0.0 3 0 0 0 3 50 2 0.0 3 0 0 1 1 51 1 0.0 3 0 0 0 1 53 3 0.0 3 0 0 0 3 54 3 0.0 3 0 0 1 2 57 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 67 38 0.0 3 0 0 0 38 68 3 0.0 3 0 0 0 3 69 3 0.0 3 0 0 0 3 70 1 0.0 3 0 0 0 1 71 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 73 2 0.0 3 0 0 0 2 74 5 0.0 3 0 0 0 5 75 4 0.0 3 0 0 0 4 76 1 0.0 3 0 0 0 1 77 4 0.0 3 0 0 1 3 78 2 0.0 3 0 0 0 2 79 3 0.0 3 0 0 0 3 80 19 0.0 3 0 0 0 19 81 2 0.0 3 0 0 0 2 83 3 0.0 3 0 0 0 3 85 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 89 4 0.0 3 0 0 0 4 91 2 0.0 3 0 0 1 1 92 1 0.0 3 0 0 0 1 93 4 0.0 3 0 0 0 4 94 2 0.0 3 0 0 1 1 99 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 103 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 112 3 0.0 3 0 0 0 3 114 2 0.0 3 0 0 0 2 115 1 0.0 3 1 116 3 0.0 3 1 0 1 1 117 2 0.0 3 0 1 1 118 4 0.0 3 0 0 0 4 119 2 0.0 3 0 0 0 2 120 1 0.0 3 0 0 0 1 121 4 0.0 3 0 0 0 4 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 127 3 0.0 3 0 0 0 3 128 2 0.0 3 0 0 0 2 129 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 131 3 0.0 3 0 0 0 3 133 4 0.0 3 0 0 0 4 134 2 0.0 3 0 0 0 2 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 164 4 0.0 3 0 0 1 3 165 3 0.0 3 0 0 0 3 166 2 0.0 3 0 0 0 2 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 173 2 0.0 3 0 0 0 2 182 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 184 3 0.0 3 0 0 0 3 186 1 0.0 3 0 0 0 1 187 3 0.0 3 0 0 1 2 190 2 0.0 3 0 0 0 2 193 2 0.0 3 0 0 0 2 194 13 0.0 3 0 0 1 12 195 1 0.0 3 0 0 0 1 197 2 0.0 3 0 0 0 2 198 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 204 3 0.0 3 0 0 0 3 205 8 0.0 3 0 0 0 8 210 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 216 2 0.0 3 0 0 0 2 217 22 0.0 3 0 0 1 21 218 5 0.0 3 0 0 0 5 219 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 1 225 1 0.0 3 0 0 0 1 237 3 0.0 3 0 0 0 3 238 2 0.0 3 0 0 0 2 239 2 0.0 3 0 0 0 2 243 2 0.0 3 0 0 0 2 244 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 270 3 0.0 3 0 0 0 3 278 2 0.0 3 0 0 0 2 279 8 0.0 3 0 0 0 8 280 8 0.0 3 0 0 0 8 281 21 0.0 3 0 0 0 21 282 7 0.0 3 0 0 0 7 283 5 0.0 3 0 0 1 4 286 1 0.0 3 0 0 0 1 288 2 0.0 3 0 0 0 2 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.80 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 80,150 Reads with adapters: 813 (1.0%) Reads written (passing filters): 80,150 (100.0%) Total basepairs processed: 22,174,178 bp Total written (filtered): 22,171,600 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 813 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 52.9% C: 24.8% G: 13.9% T: 8.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 771 1252.3 0 771 4 17 313.1 0 17 5 12 78.3 0 12 6 3 19.6 0 3 11 1 0.0 1 0 1 12 9 0.0 1 0 9