This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.79 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 220,899 (9.0%) Reads that were too short: 43,340 (1.8%) Reads written (passing filters): 177,559 (7.2%) Total basepairs processed: 740,966,583 bp Total written (filtered): 50,012,540 bp (6.7%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 220899 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1973 601.0 0 1973 7 70873 601.0 0 70873 8 85018 601.0 0 85018 9 97 601.0 0 97 10 90 601.0 0 90 11 88 601.0 0 88 12 64 601.0 0 64 13 64 601.0 0 64 14 80 601.0 0 80 15 74 601.0 0 74 16 61 601.0 0 61 17 94 601.0 0 94 18 63 601.0 0 63 19 82 601.0 0 82 20 76 601.0 0 76 21 49 601.0 0 49 22 55 601.0 0 55 23 71 601.0 0 71 24 66 601.0 0 66 25 73 601.0 0 73 26 69 601.0 0 69 27 67 601.0 0 67 28 61 601.0 0 61 29 59 601.0 0 59 30 71 601.0 0 71 31 88 601.0 0 88 32 75 601.0 0 75 33 53 601.0 0 53 34 114 601.0 0 114 35 73 601.0 0 73 36 67 601.0 0 67 37 79 601.0 0 79 38 90 601.0 0 90 39 65 601.0 0 65 40 63 601.0 0 63 41 61 601.0 0 61 42 65 601.0 0 65 43 69 601.0 0 69 44 117 601.0 0 117 45 101 601.0 0 101 46 62 601.0 0 62 47 68 601.0 0 68 48 86 601.0 0 86 49 76 601.0 0 76 50 68 601.0 0 68 51 71 601.0 0 71 52 100 601.0 0 100 53 88 601.0 0 88 54 75 601.0 0 75 55 83 601.0 0 83 56 96 601.0 0 96 57 99 601.0 0 99 58 61 601.0 0 61 59 118 601.0 0 118 60 97 601.0 0 97 61 257 601.0 0 257 62 79 601.0 0 79 63 101 601.0 0 101 64 88 601.0 0 88 65 102 601.0 0 102 66 69 601.0 0 69 67 64 601.0 0 64 68 80 601.0 0 80 69 73 601.0 0 73 70 90 601.0 0 90 71 100 601.0 0 100 72 123 601.0 0 123 73 118 601.0 0 118 74 160 601.0 0 160 75 86 601.0 0 86 76 94 601.0 0 94 77 77 601.0 0 77 78 113 601.0 0 113 79 114 601.0 0 114 80 119 601.0 0 119 81 105 601.0 0 105 82 118 601.0 0 118 83 115 601.0 0 115 84 127 601.0 0 127 85 118 601.0 0 118 86 120 601.0 0 120 87 97 601.0 0 97 88 103 601.0 0 103 89 87 601.0 0 87 90 80 601.0 0 80 91 83 601.0 0 83 92 92 601.0 0 92 93 70 601.0 0 70 94 74 601.0 0 74 95 124 601.0 0 124 96 92 601.0 0 92 97 70 601.0 0 70 98 95 601.0 0 95 99 150 601.0 0 150 100 97 601.0 0 97 101 108 601.0 0 108 102 111 601.0 0 111 103 118 601.0 0 118 104 108 601.0 0 108 105 70 601.0 0 70 106 89 601.0 0 89 107 107 601.0 0 107 108 86 601.0 0 86 109 83 601.0 0 83 110 94 601.0 0 94 111 84 601.0 0 84 112 84 601.0 0 84 113 80 601.0 0 80 114 101 601.0 0 101 115 82 601.0 0 82 116 104 601.0 0 104 117 88 601.0 0 88 118 88 601.0 0 88 119 66 601.0 0 66 120 80 601.0 0 80 121 89 601.0 0 89 122 93 601.0 0 93 123 100 601.0 0 100 124 184 601.0 0 184 125 204 601.0 0 204 126 178 601.0 0 178 127 96 601.0 0 96 128 97 601.0 0 97 129 78 601.0 0 78 130 85 601.0 0 85 131 112 601.0 0 112 132 86 601.0 0 86 133 97 601.0 0 97 134 151 601.0 0 151 135 214 601.0 0 214 136 87 601.0 0 87 137 106 601.0 0 106 138 92 601.0 0 92 139 236 601.0 0 236 140 82 601.0 0 82 141 84 601.0 0 84 142 86 601.0 0 86 143 67 601.0 0 67 144 86 601.0 0 86 145 95 601.0 0 95 146 132 601.0 0 132 147 188 601.0 0 188 148 103 601.0 0 103 149 258 601.0 0 258 150 104 601.0 0 104 151 103 601.0 0 103 152 80 601.0 0 80 153 94 601.0 0 94 154 99 601.0 0 99 155 88 601.0 0 88 156 98 601.0 0 98 157 103 601.0 0 103 158 160 601.0 0 160 159 106 601.0 0 106 160 141 601.0 0 141 161 87 601.0 0 87 162 119 601.0 0 119 163 96 601.0 0 96 164 87 601.0 0 87 165 108 601.0 0 108 166 99 601.0 0 99 167 159 601.0 0 159 168 97 601.0 0 97 169 150 601.0 0 150 170 159 601.0 0 159 171 231 601.0 0 231 172 154 601.0 0 154 173 114 601.0 0 114 174 106 601.0 0 106 175 84 601.0 0 84 176 101 601.0 0 101 177 83 601.0 0 83 178 111 601.0 0 111 179 101 601.0 0 101 180 108 601.0 0 108 181 117 601.0 0 117 182 81 601.0 0 81 183 111 601.0 0 111 184 111 601.0 0 111 185 109 601.0 0 109 186 118 601.0 0 118 187 144 601.0 0 144 188 209 601.0 0 209 189 170 601.0 0 170 190 117 601.0 0 117 191 107 601.0 0 107 192 136 601.0 0 136 193 111 601.0 0 111 194 117 601.0 0 117 195 158 601.0 0 158 196 114 601.0 0 114 197 121 601.0 0 121 198 126 601.0 0 126 199 117 601.0 0 117 200 135 601.0 0 135 201 113 601.0 0 113 202 107 601.0 0 107 203 160 601.0 0 160 204 196 601.0 0 196 205 162 601.0 0 162 206 119 601.0 0 119 207 89 601.0 0 89 208 110 601.0 0 110 209 117 601.0 0 117 210 146 601.0 0 146 211 97 601.0 0 97 212 116 601.0 0 116 213 146 601.0 0 146 214 352 601.0 0 352 215 612 601.0 0 612 216 820 601.0 0 820 217 583 601.0 0 583 218 190 601.0 0 190 219 184 601.0 0 184 220 125 601.0 0 125 221 103 601.0 0 103 222 146 601.0 0 146 223 186 601.0 0 186 224 211 601.0 0 211 225 307 601.0 0 307 226 1188 601.0 0 1188 227 3695 601.0 0 3695 228 4723 601.0 0 4723 229 6105 601.0 0 6105 230 837 601.0 0 837 231 827 601.0 0 827 232 1587 601.0 0 1587 233 1330 601.0 0 1330 234 108 601.0 0 108 235 86 601.0 0 86 236 109 601.0 0 109 237 121 601.0 0 121 238 152 601.0 0 152 239 198 601.0 0 198 240 173 601.0 0 173 241 121 601.0 0 121 242 107 601.0 0 107 243 89 601.0 0 89 244 116 601.0 0 116 245 110 601.0 0 110 246 141 601.0 0 141 247 131 601.0 0 131 248 197 601.0 0 197 249 198 601.0 0 198 250 159 601.0 0 159 251 120 601.0 0 120 252 84 601.0 0 84 253 95 601.0 0 95 254 153 601.0 0 153 255 137 601.0 0 137 256 158 601.0 0 158 257 146 601.0 0 146 258 141 601.0 0 141 259 218 601.0 0 218 260 176 601.0 0 176 261 196 601.0 0 196 262 158 601.0 0 158 263 177 601.0 0 177 264 338 601.0 0 338 265 798 601.0 0 798 266 1657 601.0 0 1657 267 1920 601.0 0 1920 268 1435 601.0 0 1435 269 230 601.0 0 230 270 205 601.0 0 205 271 164 601.0 0 164 272 144 601.0 0 144 273 180 601.0 0 180 274 221 601.0 0 221 275 269 601.0 0 269 276 318 601.0 0 318 277 166 601.0 0 166 278 183 601.0 0 183 279 215 601.0 0 215 280 232 601.0 0 232 281 176 601.0 0 176 282 221 601.0 0 221 283 163 601.0 0 163 284 150 601.0 0 150 285 151 601.0 0 151 286 196 601.0 0 196 287 243 601.0 0 243 288 174 601.0 0 174 289 183 601.0 0 183 290 145 601.0 0 145 291 813 601.0 0 813 292 541 601.0 0 541 293 483 601.0 0 483 294 139 601.0 0 139 295 101 601.0 0 101 296 110 601.0 0 110 297 98 601.0 0 98 298 109 601.0 0 109 299 132 601.0 0 132 300 154 601.0 0 154 301 232 601.0 0 232 Finished in 8.62 s (49 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 177,559 Reads with adapters: 88,427 (49.8%) Reads that were too short: 30 (0.0%) Reads written (passing filters): 88,397 (49.8%) Total basepairs processed: 50,012,540 bp Total written (filtered): 24,015,522 bp (48.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 88427 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 27 0.0 3 0 0 24 1 2 19 923 0.0 3 0 911 10 2 20 85098 0.0 4 83743 1310 41 3 1 21 190 0.0 4 34 154 2 22 16 0.0 4 1 3 10 1 1 23 1034 0.0 4 963 18 0 49 4 24 8 0.0 4 3 2 0 0 3 28 1 0.0 4 1 45 1 0.0 4 1 60 1 0.0 4 0 0 0 0 1 61 2 0.0 4 0 0 0 0 2 71 1 0.0 4 1 92 1 0.0 4 0 0 0 0 1 118 1 0.0 4 1 119 2 0.0 4 0 1 0 1 120 2 0.0 4 1 0 1 121 1 0.0 4 0 0 1 122 1 0.0 4 0 0 0 0 1 125 4 0.0 4 0 0 0 0 4 126 8 0.0 4 0 0 0 0 8 127 1017 0.0 4 0 0 0 0 1017 128 2 0.0 4 0 0 0 0 2 135 59 0.0 4 0 0 0 0 59 139 2 0.0 4 0 0 0 0 2 152 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 179 1 0.0 4 0 1 182 1 0.0 4 0 1 193 1 0.0 4 1 210 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 0 4 229 3 0.0 4 0 0 0 1 2 235 2 0.0 4 0 0 0 0 2 237 1 0.0 4 0 0 0 0 1 265 2 0.0 4 0 0 0 1 1 283 2 0.0 4 0 0 0 0 2 286 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.11 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 88,397 Reads with adapters: 9,339 (10.6%) Reads written (passing filters): 88,397 (100.0%) Total basepairs processed: 24,015,522 bp Total written (filtered): 23,913,029 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 9339 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.5% C: 3.7% G: 89.9% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 64 1381.2 0 64 4 61 345.3 0 61 5 6 86.3 0 6 8 1 1.3 0 1 9 375 0.3 0 10 365 10 1690 0.1 1 62 1628 11 6797 0.0 1 47 6750 12 23 0.0 1 1 22 14 3 0.0 1 0 3 15 2 0.0 1 0 2 19 296 0.0 1 241 55 20 16 0.0 1 12 4 21 1 0.0 1 1 23 1 0.0 1 1 208 1 0.0 1 1 209 1 0.0 1 1 224 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.