This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 63.47 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 51,718 (2.1%) Reads that were too short: 4,531 (0.2%) Reads written (passing filters): 47,187 (1.9%) Total basepairs processed: 740,966,583 bp Total written (filtered): 11,289,721 bp (1.5%) === Adapter 1 === Sequence: TGTATA; Type: regular 5'; Length: 6; Trimmed: 51718 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 737 601.0 0 737 7 14742 601.0 0 14742 8 228 601.0 0 228 9 9689 601.0 0 9689 10 8 601.0 0 8 11 2 601.0 0 2 12 3 601.0 0 3 14 4 601.0 0 4 15 3 601.0 0 3 17 1 601.0 0 1 18 3 601.0 0 3 19 1 601.0 0 1 21 1 601.0 0 1 22 1 601.0 0 1 23 1 601.0 0 1 25 1 601.0 0 1 27 2 601.0 0 2 28 6 601.0 0 6 29 6 601.0 0 6 30 1 601.0 0 1 31 1 601.0 0 1 32 1 601.0 0 1 33 4 601.0 0 4 34 2 601.0 0 2 35 6 601.0 0 6 36 3 601.0 0 3 37 4 601.0 0 4 38 1 601.0 0 1 39 4 601.0 0 4 40 3 601.0 0 3 41 8 601.0 0 8 42 2 601.0 0 2 43 8 601.0 0 8 44 1 601.0 0 1 45 7 601.0 0 7 46 18 601.0 0 18 47 7 601.0 0 7 48 5 601.0 0 5 49 2 601.0 0 2 50 8 601.0 0 8 51 9 601.0 0 9 52 6 601.0 0 6 53 6 601.0 0 6 54 7 601.0 0 7 55 2 601.0 0 2 56 1 601.0 0 1 57 16 601.0 0 16 58 4 601.0 0 4 59 4 601.0 0 4 60 3 601.0 0 3 61 2 601.0 0 2 62 3 601.0 0 3 63 2 601.0 0 2 64 2 601.0 0 2 65 3 601.0 0 3 66 5 601.0 0 5 67 3 601.0 0 3 68 7 601.0 0 7 69 3 601.0 0 3 70 5 601.0 0 5 71 3 601.0 0 3 72 4 601.0 0 4 73 2 601.0 0 2 74 3 601.0 0 3 75 2 601.0 0 2 76 4 601.0 0 4 77 10 601.0 0 10 78 26 601.0 0 26 79 8 601.0 0 8 80 3 601.0 0 3 81 4 601.0 0 4 82 12 601.0 0 12 83 13 601.0 0 13 84 1 601.0 0 1 85 11 601.0 0 11 86 23 601.0 0 23 87 20 601.0 0 20 88 13 601.0 0 13 89 18 601.0 0 18 90 6 601.0 0 6 91 13 601.0 0 13 92 11 601.0 0 11 93 23 601.0 0 23 94 16 601.0 0 16 95 22 601.0 0 22 96 24 601.0 0 24 97 37 601.0 0 37 98 74 601.0 0 74 99 371 601.0 0 371 100 197 601.0 0 197 101 123 601.0 0 123 102 30 601.0 0 30 103 27 601.0 0 27 104 37 601.0 0 37 105 20 601.0 0 20 106 39 601.0 0 39 107 29 601.0 0 29 108 65 601.0 0 65 109 118 601.0 0 118 110 148 601.0 0 148 111 4939 601.0 0 4939 112 2174 601.0 0 2174 113 1353 601.0 0 1353 114 1212 601.0 0 1212 115 484 601.0 0 484 116 8 601.0 0 8 117 10 601.0 0 10 118 19 601.0 0 19 119 15 601.0 0 15 120 13 601.0 0 13 121 13 601.0 0 13 122 12 601.0 0 12 123 5 601.0 0 5 124 6 601.0 0 6 125 21 601.0 0 21 126 13 601.0 0 13 127 140 601.0 0 140 128 30 601.0 0 30 129 168 601.0 0 168 130 78 601.0 0 78 131 48 601.0 0 48 132 37 601.0 0 37 133 39 601.0 0 39 134 22 601.0 0 22 135 247 601.0 0 247 136 251 601.0 0 251 137 130 601.0 0 130 138 23 601.0 0 23 139 53 601.0 0 53 140 68 601.0 0 68 141 120 601.0 0 120 142 2378 601.0 0 2378 143 3760 601.0 0 3760 144 1003 601.0 0 1003 145 310 601.0 0 310 146 11 601.0 0 11 147 21 601.0 0 21 148 7 601.0 0 7 149 16 601.0 0 16 150 6 601.0 0 6 151 8 601.0 0 8 152 17 601.0 0 17 153 3 601.0 0 3 154 3 601.0 0 3 155 3 601.0 0 3 156 12 601.0 0 12 157 10 601.0 0 10 158 11 601.0 0 11 159 10 601.0 0 10 160 14 601.0 0 14 161 15 601.0 0 15 162 16 601.0 0 16 163 10 601.0 0 10 164 13 601.0 0 13 165 9 601.0 0 9 166 13 601.0 0 13 167 6 601.0 0 6 168 7 601.0 0 7 169 5 601.0 0 5 170 4 601.0 0 4 171 7 601.0 0 7 172 13 601.0 0 13 173 3 601.0 0 3 174 9 601.0 0 9 175 8 601.0 0 8 176 14 601.0 0 14 177 17 601.0 0 17 178 23 601.0 0 23 179 10 601.0 0 10 180 5 601.0 0 5 181 7 601.0 0 7 182 6 601.0 0 6 183 12 601.0 0 12 184 11 601.0 0 11 185 11 601.0 0 11 186 156 601.0 0 156 187 14 601.0 0 14 188 76 601.0 0 76 189 15 601.0 0 15 190 12 601.0 0 12 191 4 601.0 0 4 192 6 601.0 0 6 193 8 601.0 0 8 194 7 601.0 0 7 195 7 601.0 0 7 196 8 601.0 0 8 197 4 601.0 0 4 198 5 601.0 0 5 199 8 601.0 0 8 200 5 601.0 0 5 201 6 601.0 0 6 202 9 601.0 0 9 203 11 601.0 0 11 204 7 601.0 0 7 205 3 601.0 0 3 206 9 601.0 0 9 207 10 601.0 0 10 208 13 601.0 0 13 209 13 601.0 0 13 210 17 601.0 0 17 211 10 601.0 0 10 212 10 601.0 0 10 213 13 601.0 0 13 214 10 601.0 0 10 215 17 601.0 0 17 216 15 601.0 0 15 217 9 601.0 0 9 218 12 601.0 0 12 219 10 601.0 0 10 220 14 601.0 0 14 221 20 601.0 0 20 222 13 601.0 0 13 223 23 601.0 0 23 224 71 601.0 0 71 225 71 601.0 0 71 226 42 601.0 0 42 227 7 601.0 0 7 228 4 601.0 0 4 229 8 601.0 0 8 230 7 601.0 0 7 231 7 601.0 0 7 232 16 601.0 0 16 233 26 601.0 0 26 234 18 601.0 0 18 235 16 601.0 0 16 236 18 601.0 0 18 237 25 601.0 0 25 238 15 601.0 0 15 239 29 601.0 0 29 240 21 601.0 0 21 241 29 601.0 0 29 242 27 601.0 0 27 243 34 601.0 0 34 244 29 601.0 0 29 245 18 601.0 0 18 246 8 601.0 0 8 247 17 601.0 0 17 248 8 601.0 0 8 249 13 601.0 0 13 250 30 601.0 0 30 251 25 601.0 0 25 252 21 601.0 0 21 253 13 601.0 0 13 254 16 601.0 0 16 255 33 601.0 0 33 256 55 601.0 0 55 257 30 601.0 0 30 258 20 601.0 0 20 259 27 601.0 0 27 260 23 601.0 0 23 261 24 601.0 0 24 262 66 601.0 0 66 263 149 601.0 0 149 264 62 601.0 0 62 265 27 601.0 0 27 266 24 601.0 0 24 267 21 601.0 0 21 268 12 601.0 0 12 269 14 601.0 0 14 270 22 601.0 0 22 271 24 601.0 0 24 272 16 601.0 0 16 273 21 601.0 0 21 274 33 601.0 0 33 275 238 601.0 0 238 276 86 601.0 0 86 277 281 601.0 0 281 278 43 601.0 0 43 279 28 601.0 0 28 280 75 601.0 0 75 281 46 601.0 0 46 282 96 601.0 0 96 283 111 601.0 0 111 284 199 601.0 0 199 285 49 601.0 0 49 286 21 601.0 0 21 287 22 601.0 0 22 288 14 601.0 0 14 289 49 601.0 0 49 290 244 601.0 0 244 291 243 601.0 0 243 292 107 601.0 0 107 293 61 601.0 0 61 294 349 601.0 0 349 295 274 601.0 0 274 296 33 601.0 0 33 297 46 601.0 0 46 298 42 601.0 0 42 299 55 601.0 0 55 300 38 601.0 0 38 301 21 601.0 0 21 Finished in 2.29 s (49 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 47,187 Reads with adapters: 15,081 (32.0%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 15,063 (31.9%) Total basepairs processed: 11,289,721 bp Total written (filtered): 4,095,601 bp (36.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 15081 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 19 111 0.0 3 0 107 4 20 14622 0.0 4 14380 233 7 2 21 22 0.0 4 3 19 22 1 0.0 4 0 0 1 23 11 0.0 4 1 0 0 9 1 26 1 0.0 4 0 0 0 1 31 1 0.0 4 0 0 0 0 1 35 7 0.0 4 0 0 0 0 7 122 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 126 6 0.0 4 0 0 0 0 6 127 278 0.0 4 0 0 0 0 278 128 6 0.0 4 0 0 0 0 6 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 1 147 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.52 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 15,063 Reads with adapters: 242 (1.6%) Reads written (passing filters): 15,063 (100.0%) Total basepairs processed: 4,095,601 bp Total written (filtered): 4,092,985 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 242 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 9.5% C: 7.0% G: 81.8% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 26 235.4 0 26 4 5 58.8 0 5 5 1 14.7 0 1 6 1 3.7 0 1 9 3 0.1 0 1 2 10 2 0.0 1 0 2 11 160 0.0 1 0 160 12 40 0.0 1 0 40 13 1 0.0 1 0 1 14 1 0.0 1 0 1 15 1 0.0 1 0 1 178 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.