This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.65 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 165,033 (6.7%) Reads that were too short: 13,634 (0.6%) Reads written (passing filters): 151,399 (6.2%) Total basepairs processed: 740,966,583 bp Total written (filtered): 41,836,182 bp (5.6%) === Adapter 1 === Sequence: CTCATG; Type: regular 5'; Length: 6; Trimmed: 165033 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1690 601.0 0 1690 7 67842 601.0 0 67842 8 2074 601.0 0 2074 9 55757 601.0 0 55757 10 97 601.0 0 97 11 65 601.0 0 65 12 66 601.0 0 66 13 72 601.0 0 72 14 85 601.0 0 85 15 46 601.0 0 46 16 53 601.0 0 53 17 54 601.0 0 54 18 77 601.0 0 77 19 38 601.0 0 38 20 48 601.0 0 48 21 45 601.0 0 45 22 28 601.0 0 28 23 37 601.0 0 37 24 45 601.0 0 45 25 45 601.0 0 45 26 40 601.0 0 40 27 52 601.0 0 52 28 60 601.0 0 60 29 76 601.0 0 76 30 92 601.0 0 92 31 48 601.0 0 48 32 67 601.0 0 67 33 114 601.0 0 114 34 49 601.0 0 49 35 46 601.0 0 46 36 50 601.0 0 50 37 62 601.0 0 62 38 80 601.0 0 80 39 83 601.0 0 83 40 80 601.0 0 80 41 104 601.0 0 104 42 64 601.0 0 64 43 81 601.0 0 81 44 145 601.0 0 145 45 177 601.0 0 177 46 509 601.0 0 509 47 124 601.0 0 124 48 41 601.0 0 41 49 62 601.0 0 62 50 51 601.0 0 51 51 99 601.0 0 99 52 55 601.0 0 55 53 88 601.0 0 88 54 68 601.0 0 68 55 62 601.0 0 62 56 146 601.0 0 146 57 362 601.0 0 362 58 1275 601.0 0 1275 59 428 601.0 0 428 60 136 601.0 0 136 61 57 601.0 0 57 62 52 601.0 0 52 63 30 601.0 0 30 64 52 601.0 0 52 65 48 601.0 0 48 66 52 601.0 0 52 67 51 601.0 0 51 68 69 601.0 0 69 69 37 601.0 0 37 70 96 601.0 0 96 71 43 601.0 0 43 72 52 601.0 0 52 73 81 601.0 0 81 74 68 601.0 0 68 75 67 601.0 0 67 76 74 601.0 0 74 77 65 601.0 0 65 78 52 601.0 0 52 79 66 601.0 0 66 80 56 601.0 0 56 81 64 601.0 0 64 82 57 601.0 0 57 83 70 601.0 0 70 84 70 601.0 0 70 85 52 601.0 0 52 86 64 601.0 0 64 87 46 601.0 0 46 88 57 601.0 0 57 89 51 601.0 0 51 90 49 601.0 0 49 91 99 601.0 0 99 92 116 601.0 0 116 93 94 601.0 0 94 94 76 601.0 0 76 95 53 601.0 0 53 96 60 601.0 0 60 97 79 601.0 0 79 98 76 601.0 0 76 99 76 601.0 0 76 100 51 601.0 0 51 101 65 601.0 0 65 102 65 601.0 0 65 103 57 601.0 0 57 104 72 601.0 0 72 105 93 601.0 0 93 106 335 601.0 0 335 107 471 601.0 0 471 108 871 601.0 0 871 109 98 601.0 0 98 110 125 601.0 0 125 111 70 601.0 0 70 112 55 601.0 0 55 113 119 601.0 0 119 114 254 601.0 0 254 115 156 601.0 0 156 116 79 601.0 0 79 117 51 601.0 0 51 118 55 601.0 0 55 119 73 601.0 0 73 120 48 601.0 0 48 121 68 601.0 0 68 122 47 601.0 0 47 123 77 601.0 0 77 124 77 601.0 0 77 125 99 601.0 0 99 126 77 601.0 0 77 127 106 601.0 0 106 128 225 601.0 0 225 129 280 601.0 0 280 130 126 601.0 0 126 131 75 601.0 0 75 132 60 601.0 0 60 133 92 601.0 0 92 134 131 601.0 0 131 135 220 601.0 0 220 136 349 601.0 0 349 137 235 601.0 0 235 138 174 601.0 0 174 139 165 601.0 0 165 140 136 601.0 0 136 141 222 601.0 0 222 142 224 601.0 0 224 143 113 601.0 0 113 144 72 601.0 0 72 145 73 601.0 0 73 146 95 601.0 0 95 147 96 601.0 0 96 148 1220 601.0 0 1220 149 417 601.0 0 417 150 1109 601.0 0 1109 151 49 601.0 0 49 152 47 601.0 0 47 153 53 601.0 0 53 154 57 601.0 0 57 155 201 601.0 0 201 156 270 601.0 0 270 157 342 601.0 0 342 158 116 601.0 0 116 159 71 601.0 0 71 160 80 601.0 0 80 161 80 601.0 0 80 162 124 601.0 0 124 163 361 601.0 0 361 164 630 601.0 0 630 165 839 601.0 0 839 166 72 601.0 0 72 167 47 601.0 0 47 168 70 601.0 0 70 169 112 601.0 0 112 170 111 601.0 0 111 171 39 601.0 0 39 172 41 601.0 0 41 173 29 601.0 0 29 174 63 601.0 0 63 175 37 601.0 0 37 176 31 601.0 0 31 177 41 601.0 0 41 178 45 601.0 0 45 179 68 601.0 0 68 180 161 601.0 0 161 181 194 601.0 0 194 182 207 601.0 0 207 183 119 601.0 0 119 184 73 601.0 0 73 185 87 601.0 0 87 186 74 601.0 0 74 187 40 601.0 0 40 188 39 601.0 0 39 189 52 601.0 0 52 190 58 601.0 0 58 191 72 601.0 0 72 192 58 601.0 0 58 193 82 601.0 0 82 194 38 601.0 0 38 195 43 601.0 0 43 196 57 601.0 0 57 197 54 601.0 0 54 198 62 601.0 0 62 199 66 601.0 0 66 200 45 601.0 0 45 201 52 601.0 0 52 202 231 601.0 0 231 203 99 601.0 0 99 204 201 601.0 0 201 205 39 601.0 0 39 206 54 601.0 0 54 207 48 601.0 0 48 208 79 601.0 0 79 209 77 601.0 0 77 210 56 601.0 0 56 211 42 601.0 0 42 212 49 601.0 0 49 213 100 601.0 0 100 214 99 601.0 0 99 215 105 601.0 0 105 216 96 601.0 0 96 217 66 601.0 0 66 218 65 601.0 0 65 219 38 601.0 0 38 220 61 601.0 0 61 221 54 601.0 0 54 222 58 601.0 0 58 223 65 601.0 0 65 224 59 601.0 0 59 225 54 601.0 0 54 226 48 601.0 0 48 227 50 601.0 0 50 228 62 601.0 0 62 229 50 601.0 0 50 230 48 601.0 0 48 231 41 601.0 0 41 232 110 601.0 0 110 233 189 601.0 0 189 234 178 601.0 0 178 235 107 601.0 0 107 236 75 601.0 0 75 237 55 601.0 0 55 238 79 601.0 0 79 239 60 601.0 0 60 240 46 601.0 0 46 241 44 601.0 0 44 242 104 601.0 0 104 243 71 601.0 0 71 244 110 601.0 0 110 245 83 601.0 0 83 246 70 601.0 0 70 247 61 601.0 0 61 248 105 601.0 0 105 249 58 601.0 0 58 250 58 601.0 0 58 251 100 601.0 0 100 252 111 601.0 0 111 253 159 601.0 0 159 254 94 601.0 0 94 255 112 601.0 0 112 256 92 601.0 0 92 257 78 601.0 0 78 258 81 601.0 0 81 259 95 601.0 0 95 260 60 601.0 0 60 261 129 601.0 0 129 262 189 601.0 0 189 263 111 601.0 0 111 264 88 601.0 0 88 265 92 601.0 0 92 266 94 601.0 0 94 267 66 601.0 0 66 268 67 601.0 0 67 269 103 601.0 0 103 270 207 601.0 0 207 271 818 601.0 0 818 272 338 601.0 0 338 273 256 601.0 0 256 274 71 601.0 0 71 275 85 601.0 0 85 276 90 601.0 0 90 277 89 601.0 0 89 278 104 601.0 0 104 279 155 601.0 0 155 280 144 601.0 0 144 281 114 601.0 0 114 282 92 601.0 0 92 283 154 601.0 0 154 284 122 601.0 0 122 285 139 601.0 0 139 286 75 601.0 0 75 287 71 601.0 0 71 288 95 601.0 0 95 289 85 601.0 0 85 290 136 601.0 0 136 291 127 601.0 0 127 292 199 601.0 0 199 293 133 601.0 0 133 294 229 601.0 0 229 295 898 601.0 0 898 296 1117 601.0 0 1117 297 1143 601.0 0 1143 298 368 601.0 0 368 299 67 601.0 0 67 300 70 601.0 0 70 301 65 601.0 0 65 Finished in 7.06 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 151,399 Reads with adapters: 68,968 (45.6%) Reads that were too short: 369 (0.2%) Reads written (passing filters): 68,599 (45.3%) Total basepairs processed: 41,836,182 bp Total written (filtered): 18,958,577 bp (45.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 68968 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 20 0.0 2 0 0 0 20 15 45 0.0 3 0 0 24 21 16 598 0.0 3 0 448 89 61 17 66980 0.0 3 61137 4672 929 242 18 184 0.0 3 19 134 17 14 19 278 0.0 3 0 0 148 130 20 206 0.0 3 0 0 1 205 21 1 0.0 3 0 0 1 27 6 0.0 3 0 0 1 5 30 8 0.0 3 0 0 1 7 33 3 0.0 3 0 0 1 2 35 2 0.0 3 0 0 0 2 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 38 2 0.0 3 0 0 0 2 41 2 0.0 3 0 0 0 2 43 77 0.0 3 0 0 0 77 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 14 0.0 3 0 0 0 14 48 4 0.0 3 0 0 0 4 50 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 53 4 0.0 3 0 0 1 3 54 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 61 4 0.0 3 0 0 0 4 63 1 0.0 3 0 0 0 1 64 19 0.0 3 0 0 0 19 65 6 0.0 3 0 0 0 6 66 3 0.0 3 0 0 0 3 67 1 0.0 3 0 0 0 1 69 11 0.0 3 0 0 0 11 70 8 0.0 3 0 0 0 8 71 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 73 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 2 0.0 3 0 0 0 2 80 2 0.0 3 0 0 0 2 81 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 1 83 3 0.0 3 0 0 0 3 85 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 89 1 0.0 3 0 0 0 1 90 2 0.0 3 1 0 0 1 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 103 6 0.0 3 0 0 0 6 104 1 0.0 3 0 0 0 1 107 4 0.0 3 0 0 0 4 108 4 0.0 3 0 0 0 4 110 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 18 0.0 3 0 0 0 18 114 3 0.0 3 0 0 0 3 115 1 0.0 3 0 0 0 1 116 8 0.0 3 0 0 0 8 117 2 0.0 3 0 0 0 2 119 2 0.0 3 0 0 0 2 120 1 0.0 3 0 0 1 122 2 0.0 3 0 0 0 2 124 3 0.0 3 0 0 0 3 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 0 1 133 3 0.0 3 0 0 0 3 134 5 0.0 3 0 0 1 4 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 1 139 4 0.0 3 0 0 0 4 140 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 5 0.0 3 0 0 0 5 145 9 0.0 3 0 0 1 8 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 154 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 3 0.0 3 0 0 1 2 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 4 0.0 3 0 0 0 4 165 4 0.0 3 0 0 1 3 167 2 0.0 3 0 0 0 2 168 3 0.0 3 0 0 0 3 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 174 2 0.0 3 0 0 0 2 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 0 2 183 3 0.0 3 0 0 0 3 184 5 0.0 3 0 0 0 5 185 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 6 0.0 3 0 0 0 6 194 4 0.0 3 0 0 0 4 195 2 0.0 3 0 0 0 2 202 1 0.0 3 0 0 0 1 204 2 0.0 3 0 0 0 2 205 4 0.0 3 0 0 0 4 206 1 0.0 3 0 0 0 1 215 2 0.0 3 0 0 0 2 216 9 0.0 3 0 0 0 9 217 198 0.0 3 0 1 10 187 218 7 0.0 3 0 0 0 7 219 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 222 2 0.0 3 0 0 0 2 225 2 0.0 3 0 0 1 1 226 3 0.0 3 0 0 0 3 227 1 0.0 3 0 0 1 228 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 230 3 0.0 3 0 0 0 3 231 1 0.0 3 0 0 0 1 233 2 0.0 3 0 0 0 2 234 1 0.0 3 0 0 0 1 235 2 0.0 3 0 0 0 2 236 2 0.0 3 0 0 0 2 238 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 244 1 0.0 3 1 267 2 0.0 3 0 0 0 2 270 4 0.0 3 0 0 0 4 275 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 8 0.0 3 0 0 0 8 282 4 0.0 3 0 0 0 4 283 2 0.0 3 0 0 0 2 286 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.40 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 68,599 Reads with adapters: 440 (0.6%) Reads written (passing filters): 68,599 (100.0%) Total basepairs processed: 18,958,577 bp Total written (filtered): 18,957,203 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 440 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 37.0% C: 28.9% G: 19.8% T: 14.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 405 1071.9 0 405 4 21 268.0 0 21 5 9 67.0 0 9 6 5 16.7 0 5