This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.56 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 155,550 (6.3%) Reads that were too short: 6,931 (0.3%) Reads written (passing filters): 148,619 (6.0%) Total basepairs processed: 740,966,583 bp Total written (filtered): 41,983,718 bp (5.7%) === Adapter 1 === Sequence: CTCATG; Type: regular 5'; Length: 6; Trimmed: 155550 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1024 601.0 0 1024 7 64355 601.0 0 64355 8 2211 601.0 0 2211 9 66859 601.0 0 66859 10 69 601.0 0 69 11 35 601.0 0 35 12 30 601.0 0 30 13 31 601.0 0 31 14 29 601.0 0 29 15 12 601.0 0 12 16 30 601.0 0 30 17 36 601.0 0 36 18 40 601.0 0 40 19 22 601.0 0 22 20 20 601.0 0 20 21 18 601.0 0 18 22 15 601.0 0 15 23 32 601.0 0 32 24 18 601.0 0 18 25 31 601.0 0 31 26 27 601.0 0 27 27 35 601.0 0 35 28 27 601.0 0 27 29 34 601.0 0 34 30 24 601.0 0 24 31 26 601.0 0 26 32 23 601.0 0 23 33 42 601.0 0 42 34 32 601.0 0 32 35 34 601.0 0 34 36 27 601.0 0 27 37 29 601.0 0 29 38 37 601.0 0 37 39 30 601.0 0 30 40 54 601.0 0 54 41 91 601.0 0 91 42 38 601.0 0 38 43 49 601.0 0 49 44 71 601.0 0 71 45 108 601.0 0 108 46 176 601.0 0 176 47 115 601.0 0 115 48 22 601.0 0 22 49 19 601.0 0 19 50 27 601.0 0 27 51 44 601.0 0 44 52 46 601.0 0 46 53 46 601.0 0 46 54 44 601.0 0 44 55 26 601.0 0 26 56 106 601.0 0 106 57 261 601.0 0 261 58 121 601.0 0 121 59 61 601.0 0 61 60 28 601.0 0 28 61 40 601.0 0 40 62 26 601.0 0 26 63 33 601.0 0 33 64 39 601.0 0 39 65 34 601.0 0 34 66 35 601.0 0 35 67 35 601.0 0 35 68 41 601.0 0 41 69 30 601.0 0 30 70 75 601.0 0 75 71 32 601.0 0 32 72 27 601.0 0 27 73 53 601.0 0 53 74 43 601.0 0 43 75 48 601.0 0 48 76 70 601.0 0 70 77 35 601.0 0 35 78 37 601.0 0 37 79 56 601.0 0 56 80 31 601.0 0 31 81 55 601.0 0 55 82 43 601.0 0 43 83 46 601.0 0 46 84 41 601.0 0 41 85 52 601.0 0 52 86 57 601.0 0 57 87 41 601.0 0 41 88 39 601.0 0 39 89 40 601.0 0 40 90 37 601.0 0 37 91 71 601.0 0 71 92 58 601.0 0 58 93 53 601.0 0 53 94 41 601.0 0 41 95 38 601.0 0 38 96 52 601.0 0 52 97 41 601.0 0 41 98 48 601.0 0 48 99 48 601.0 0 48 100 45 601.0 0 45 101 37 601.0 0 37 102 42 601.0 0 42 103 52 601.0 0 52 104 46 601.0 0 46 105 64 601.0 0 64 106 229 601.0 0 229 107 278 601.0 0 278 108 535 601.0 0 535 109 46 601.0 0 46 110 88 601.0 0 88 111 51 601.0 0 51 112 42 601.0 0 42 113 80 601.0 0 80 114 327 601.0 0 327 115 177 601.0 0 177 116 61 601.0 0 61 117 49 601.0 0 49 118 32 601.0 0 32 119 48 601.0 0 48 120 44 601.0 0 44 121 43 601.0 0 43 122 34 601.0 0 34 123 54 601.0 0 54 124 53 601.0 0 53 125 53 601.0 0 53 126 32 601.0 0 32 127 45 601.0 0 45 128 81 601.0 0 81 129 98 601.0 0 98 130 78 601.0 0 78 131 60 601.0 0 60 132 45 601.0 0 45 133 58 601.0 0 58 134 93 601.0 0 93 135 128 601.0 0 128 136 248 601.0 0 248 137 245 601.0 0 245 138 152 601.0 0 152 139 134 601.0 0 134 140 80 601.0 0 80 141 149 601.0 0 149 142 211 601.0 0 211 143 69 601.0 0 69 144 50 601.0 0 50 145 33 601.0 0 33 146 61 601.0 0 61 147 49 601.0 0 49 148 892 601.0 0 892 149 315 601.0 0 315 150 958 601.0 0 958 151 32 601.0 0 32 152 33 601.0 0 33 153 49 601.0 0 49 154 41 601.0 0 41 155 101 601.0 0 101 156 162 601.0 0 162 157 131 601.0 0 131 158 101 601.0 0 101 159 40 601.0 0 40 160 23 601.0 0 23 161 53 601.0 0 53 162 67 601.0 0 67 163 155 601.0 0 155 164 206 601.0 0 206 165 236 601.0 0 236 166 60 601.0 0 60 167 35 601.0 0 35 168 39 601.0 0 39 169 40 601.0 0 40 170 39 601.0 0 39 171 23 601.0 0 23 172 31 601.0 0 31 173 30 601.0 0 30 174 24 601.0 0 24 175 35 601.0 0 35 176 25 601.0 0 25 177 41 601.0 0 41 178 32 601.0 0 32 179 49 601.0 0 49 180 57 601.0 0 57 181 216 601.0 0 216 182 72 601.0 0 72 183 46 601.0 0 46 184 33 601.0 0 33 185 44 601.0 0 44 186 37 601.0 0 37 187 26 601.0 0 26 188 24 601.0 0 24 189 35 601.0 0 35 190 41 601.0 0 41 191 52 601.0 0 52 192 33 601.0 0 33 193 36 601.0 0 36 194 37 601.0 0 37 195 37 601.0 0 37 196 52 601.0 0 52 197 49 601.0 0 49 198 32 601.0 0 32 199 68 601.0 0 68 200 40 601.0 0 40 201 27 601.0 0 27 202 226 601.0 0 226 203 89 601.0 0 89 204 107 601.0 0 107 205 33 601.0 0 33 206 33 601.0 0 33 207 25 601.0 0 25 208 26 601.0 0 26 209 52 601.0 0 52 210 41 601.0 0 41 211 33 601.0 0 33 212 48 601.0 0 48 213 49 601.0 0 49 214 63 601.0 0 63 215 51 601.0 0 51 216 61 601.0 0 61 217 55 601.0 0 55 218 31 601.0 0 31 219 22 601.0 0 22 220 40 601.0 0 40 221 43 601.0 0 43 222 37 601.0 0 37 223 45 601.0 0 45 224 33 601.0 0 33 225 35 601.0 0 35 226 31 601.0 0 31 227 29 601.0 0 29 228 39 601.0 0 39 229 33 601.0 0 33 230 28 601.0 0 28 231 39 601.0 0 39 232 68 601.0 0 68 233 66 601.0 0 66 234 58 601.0 0 58 235 43 601.0 0 43 236 61 601.0 0 61 237 36 601.0 0 36 238 58 601.0 0 58 239 35 601.0 0 35 240 40 601.0 0 40 241 42 601.0 0 42 242 48 601.0 0 48 243 37 601.0 0 37 244 56 601.0 0 56 245 48 601.0 0 48 246 26 601.0 0 26 247 65 601.0 0 65 248 42 601.0 0 42 249 48 601.0 0 48 250 49 601.0 0 49 251 44 601.0 0 44 252 69 601.0 0 69 253 66 601.0 0 66 254 54 601.0 0 54 255 47 601.0 0 47 256 55 601.0 0 55 257 42 601.0 0 42 258 47 601.0 0 47 259 48 601.0 0 48 260 43 601.0 0 43 261 61 601.0 0 61 262 78 601.0 0 78 263 67 601.0 0 67 264 45 601.0 0 45 265 51 601.0 0 51 266 56 601.0 0 56 267 42 601.0 0 42 268 37 601.0 0 37 269 57 601.0 0 57 270 133 601.0 0 133 271 283 601.0 0 283 272 200 601.0 0 200 273 121 601.0 0 121 274 93 601.0 0 93 275 102 601.0 0 102 276 64 601.0 0 64 277 49 601.0 0 49 278 63 601.0 0 63 279 87 601.0 0 87 280 78 601.0 0 78 281 64 601.0 0 64 282 49 601.0 0 49 283 62 601.0 0 62 284 70 601.0 0 70 285 68 601.0 0 68 286 47 601.0 0 47 287 47 601.0 0 47 288 58 601.0 0 58 289 44 601.0 0 44 290 61 601.0 0 61 291 57 601.0 0 57 292 70 601.0 0 70 293 76 601.0 0 76 294 110 601.0 0 110 295 284 601.0 0 284 296 359 601.0 0 359 297 423 601.0 0 423 298 141 601.0 0 141 299 59 601.0 0 59 300 43 601.0 0 43 301 54 601.0 0 54 Finished in 7.43 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 148,619 Reads with adapters: 71,124 (47.9%) Reads that were too short: 27 (0.0%) Reads written (passing filters): 71,097 (47.8%) Total basepairs processed: 41,983,718 bp Total written (filtered): 19,203,989 bp (45.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 71124 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 33 0.0 3 0 0 32 0 1 19 594 0.0 3 0 581 12 1 20 68917 0.0 4 67788 1090 38 0 1 21 167 0.0 4 36 128 3 22 12 0.0 4 0 1 9 0 2 23 61 0.0 4 2 0 0 53 6 24 2 0.0 4 0 0 0 0 2 26 1 0.0 4 1 28 1 0.0 4 0 1 43 1 0.0 4 0 0 0 0 1 83 1 0.0 4 1 95 1 0.0 4 0 0 0 0 1 117 1 0.0 4 1 120 1 0.0 4 1 121 1 0.0 4 1 125 3 0.0 4 0 0 0 0 3 126 13 0.0 4 0 0 0 0 13 127 1282 0.0 4 0 0 0 0 1282 130 4 0.0 4 0 0 0 0 4 141 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 1 157 1 0.0 4 1 158 1 0.0 4 0 1 170 1 0.0 4 0 1 173 1 0.0 4 0 1 202 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 2 0.0 4 0 0 0 0 2 230 2 0.0 4 0 0 0 0 2 234 1 0.0 4 0 0 0 0 1 235 2 0.0 4 0 0 0 0 2 237 1 0.0 4 0 0 0 0 1 239 1 0.0 4 1 240 1 0.0 4 0 0 1 246 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 285 1 0.0 4 1 287 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.57 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 71,097 Reads with adapters: 10,709 (15.1%) Reads written (passing filters): 71,097 (100.0%) Total basepairs processed: 19,203,989 bp Total written (filtered): 19,095,210 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 10709 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.8% C: 3.0% G: 95.2% T: 1.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 20 1110.9 0 20 4 12 277.7 0 12 5 2 69.4 0 2 6 20 17.4 0 20 7 1 4.3 0 1 8 2 1.1 0 2 9 5011 0.3 0 160 4851 10 4783 0.1 1 141 4642 11 795 0.0 1 3 792 12 10 0.0 1 0 10 13 9 0.0 1 0 9 19 2 0.0 1 2 32 1 0.0 1 1 69 1 0.0 1 1 89 1 0.0 1 0 1 122 19 0.0 1 17 2 123 1 0.0 1 1 150 1 0.0 1 0 1 177 2 0.0 1 2 208 1 0.0 1 0 1 215 14 0.0 1 11 3 216 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.