This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCGGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.67 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 529,037 (21.5%) Reads that were too short: 34,190 (1.4%) Reads written (passing filters): 494,847 (20.1%) Total basepairs processed: 740,966,583 bp Total written (filtered): 115,196,386 bp (15.5%) === Adapter 1 === Sequence: TTCGGA; Type: regular 5'; Length: 6; Trimmed: 529037 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1162 601.0 0 1162 7 4071 601.0 0 4071 8 93422 601.0 0 93422 9 75654 601.0 0 75654 10 155 601.0 0 155 11 545 601.0 0 545 12 482 601.0 0 482 13 35 601.0 0 35 14 31 601.0 0 31 15 35 601.0 0 35 16 45 601.0 0 45 17 44 601.0 0 44 18 27 601.0 0 27 19 30 601.0 0 30 20 26 601.0 0 26 21 39 601.0 0 39 22 33 601.0 0 33 23 53 601.0 0 53 24 51 601.0 0 51 25 43 601.0 0 43 26 60 601.0 0 60 27 52 601.0 0 52 28 48 601.0 0 48 29 37 601.0 0 37 30 51 601.0 0 51 31 51 601.0 0 51 32 73 601.0 0 73 33 33 601.0 0 33 34 33 601.0 0 33 35 31 601.0 0 31 36 40 601.0 0 40 37 27 601.0 0 27 38 71 601.0 0 71 39 319 601.0 0 319 40 260 601.0 0 260 41 129 601.0 0 129 42 58 601.0 0 58 43 59 601.0 0 59 44 25 601.0 0 25 45 39 601.0 0 39 46 25 601.0 0 25 47 53 601.0 0 53 48 95 601.0 0 95 49 88 601.0 0 88 50 66 601.0 0 66 51 71 601.0 0 71 52 106 601.0 0 106 53 124 601.0 0 124 54 160 601.0 0 160 55 36 601.0 0 36 56 31 601.0 0 31 57 72 601.0 0 72 58 60 601.0 0 60 59 37 601.0 0 37 60 70 601.0 0 70 61 49 601.0 0 49 62 62 601.0 0 62 63 83 601.0 0 83 64 36 601.0 0 36 65 65 601.0 0 65 66 99 601.0 0 99 67 74 601.0 0 74 68 232 601.0 0 232 69 91 601.0 0 91 70 64 601.0 0 64 71 68 601.0 0 68 72 170 601.0 0 170 73 129 601.0 0 129 74 152 601.0 0 152 75 167 601.0 0 167 76 274 601.0 0 274 77 421 601.0 0 421 78 799 601.0 0 799 79 1468 601.0 0 1468 80 435 601.0 0 435 81 560 601.0 0 560 82 967 601.0 0 967 83 492 601.0 0 492 84 405 601.0 0 405 85 811 601.0 0 811 86 965 601.0 0 965 87 856 601.0 0 856 88 1084 601.0 0 1084 89 1702 601.0 0 1702 90 2650 601.0 0 2650 91 5251 601.0 0 5251 92 145670 601.0 0 145670 93 56804 601.0 0 56804 94 62532 601.0 0 62532 95 274 601.0 0 274 96 28 601.0 0 28 97 67 601.0 0 67 98 121 601.0 0 121 99 41 601.0 0 41 100 31 601.0 0 31 101 29 601.0 0 29 102 38 601.0 0 38 103 16 601.0 0 16 104 30 601.0 0 30 105 16 601.0 0 16 106 24 601.0 0 24 107 18 601.0 0 18 108 42 601.0 0 42 109 42 601.0 0 42 110 165 601.0 0 165 111 75 601.0 0 75 112 85 601.0 0 85 113 25 601.0 0 25 114 80 601.0 0 80 115 99 601.0 0 99 116 68 601.0 0 68 117 70 601.0 0 70 118 46 601.0 0 46 119 43 601.0 0 43 120 13 601.0 0 13 121 14 601.0 0 14 122 15 601.0 0 15 123 22 601.0 0 22 124 29 601.0 0 29 125 46 601.0 0 46 126 27 601.0 0 27 127 24 601.0 0 24 128 36 601.0 0 36 129 63 601.0 0 63 130 65 601.0 0 65 131 210 601.0 0 210 132 46 601.0 0 46 133 29 601.0 0 29 134 26 601.0 0 26 135 28 601.0 0 28 136 48 601.0 0 48 137 54 601.0 0 54 138 67 601.0 0 67 139 53 601.0 0 53 140 52 601.0 0 52 141 32 601.0 0 32 142 31 601.0 0 31 143 30 601.0 0 30 144 41 601.0 0 41 145 28 601.0 0 28 146 53 601.0 0 53 147 17 601.0 0 17 148 35 601.0 0 35 149 34 601.0 0 34 150 28 601.0 0 28 151 42 601.0 0 42 152 30 601.0 0 30 153 23 601.0 0 23 154 25 601.0 0 25 155 47 601.0 0 47 156 29 601.0 0 29 157 51 601.0 0 51 158 50 601.0 0 50 159 38 601.0 0 38 160 19 601.0 0 19 161 26 601.0 0 26 162 23 601.0 0 23 163 20 601.0 0 20 164 27 601.0 0 27 165 26 601.0 0 26 166 21 601.0 0 21 167 28 601.0 0 28 168 22 601.0 0 22 169 35 601.0 0 35 170 28 601.0 0 28 171 44 601.0 0 44 172 28 601.0 0 28 173 21 601.0 0 21 174 29 601.0 0 29 175 38 601.0 0 38 176 36 601.0 0 36 177 86 601.0 0 86 178 224 601.0 0 224 179 261 601.0 0 261 180 257 601.0 0 257 181 86 601.0 0 86 182 38 601.0 0 38 183 80 601.0 0 80 184 59 601.0 0 59 185 68 601.0 0 68 186 158 601.0 0 158 187 369 601.0 0 369 188 508 601.0 0 508 189 128 601.0 0 128 190 183 601.0 0 183 191 328 601.0 0 328 192 136 601.0 0 136 193 127 601.0 0 127 194 214 601.0 0 214 195 184 601.0 0 184 196 174 601.0 0 174 197 221 601.0 0 221 198 368 601.0 0 368 199 437 601.0 0 437 200 930 601.0 0 930 201 21288 601.0 0 21288 202 6762 601.0 0 6762 203 9027 601.0 0 9027 204 440 601.0 0 440 205 418 601.0 0 418 206 1258 601.0 0 1258 207 1117 601.0 0 1117 208 353 601.0 0 353 209 143 601.0 0 143 210 79 601.0 0 79 211 115 601.0 0 115 212 201 601.0 0 201 213 295 601.0 0 295 214 77 601.0 0 77 215 66 601.0 0 66 216 203 601.0 0 203 217 128 601.0 0 128 218 84 601.0 0 84 219 91 601.0 0 91 220 61 601.0 0 61 221 169 601.0 0 169 222 162 601.0 0 162 223 201 601.0 0 201 224 91 601.0 0 91 225 507 601.0 0 507 226 936 601.0 0 936 227 1379 601.0 0 1379 228 531 601.0 0 531 229 526 601.0 0 526 230 46 601.0 0 46 231 26 601.0 0 26 232 29 601.0 0 29 233 39 601.0 0 39 234 33 601.0 0 33 235 26 601.0 0 26 236 87 601.0 0 87 237 78 601.0 0 78 238 67 601.0 0 67 239 25 601.0 0 25 240 11 601.0 0 11 241 30 601.0 0 30 242 40 601.0 0 40 243 30 601.0 0 30 244 34 601.0 0 34 245 55 601.0 0 55 246 51 601.0 0 51 247 60 601.0 0 60 248 50 601.0 0 50 249 45 601.0 0 45 250 63 601.0 0 63 251 49 601.0 0 49 252 29 601.0 0 29 253 43 601.0 0 43 254 39 601.0 0 39 255 42 601.0 0 42 256 46 601.0 0 46 257 62 601.0 0 62 258 66 601.0 0 66 259 252 601.0 0 252 260 607 601.0 0 607 261 913 601.0 0 913 262 679 601.0 0 679 263 251 601.0 0 251 264 270 601.0 0 270 265 66 601.0 0 66 266 39 601.0 0 39 267 38 601.0 0 38 268 29 601.0 0 29 269 47 601.0 0 47 270 266 601.0 0 266 271 171 601.0 0 171 272 242 601.0 0 242 273 87 601.0 0 87 274 34 601.0 0 34 275 51 601.0 0 51 276 156 601.0 0 156 277 92 601.0 0 92 278 75 601.0 0 75 279 54 601.0 0 54 280 122 601.0 0 122 281 122 601.0 0 122 282 158 601.0 0 158 283 84 601.0 0 84 284 121 601.0 0 121 285 109 601.0 0 109 286 233 601.0 0 233 287 66 601.0 0 66 288 86 601.0 0 86 289 38 601.0 0 38 290 36 601.0 0 36 291 27 601.0 0 27 292 43 601.0 0 43 293 70 601.0 0 70 294 56 601.0 0 56 295 71 601.0 0 71 296 112 601.0 0 112 297 850 601.0 0 850 298 224 601.0 0 224 299 368 601.0 0 368 300 34 601.0 0 34 301 20 601.0 0 20 Finished in 20.24 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 494,847 Reads with adapters: 101,552 (20.5%) Reads that were too short: 1,106 (0.2%) Reads written (passing filters): 100,446 (20.3%) Total basepairs processed: 115,196,386 bp Total written (filtered): 26,525,832 bp (23.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 101552 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 22 0.0 2 0 0 0 22 15 102 0.0 3 0 0 57 45 16 687 0.0 3 0 510 90 87 17 91514 0.0 3 83016 6725 1432 341 18 292 0.0 3 18 207 39 28 19 482 0.0 3 7 5 257 213 20 85 0.0 3 2 0 1 82 24 1 0.0 3 1 25 2 0.0 3 0 0 0 2 26 3 0.0 3 1 0 0 2 28 1 0.0 3 0 0 0 1 30 17 0.0 3 0 0 0 17 31 10 0.0 3 1 0 1 8 34 4 0.0 3 0 0 1 3 37 1 0.0 3 0 0 0 1 38 2 0.0 3 0 0 1 1 42 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 45 3 0.0 3 0 0 0 3 47 1 0.0 3 0 0 0 1 48 3 0.0 3 0 0 1 2 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 56 3 0.0 3 0 0 0 3 57 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 61 2 0.0 3 0 0 0 2 62 5 0.0 3 1 0 0 4 63 1 0.0 3 0 0 0 1 65 10 0.0 3 0 0 0 10 66 11 0.0 3 0 0 0 11 67 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 74 76 0.0 3 0 0 0 76 75 9 0.0 3 0 0 0 9 76 7 0.0 3 0 0 0 7 77 30 0.0 3 0 0 1 29 78 3 0.0 3 0 0 0 3 79 103 0.0 3 0 0 1 102 80 4 0.0 3 0 0 0 4 81 9 0.0 3 0 0 0 9 82 8 0.0 3 0 1 2 5 83 1 0.0 3 0 0 0 1 84 4 0.0 3 0 0 0 4 85 3 0.0 3 0 0 0 3 87 4 0.0 3 0 0 0 4 89 7 0.0 3 0 0 0 7 90 9 0.0 3 0 0 0 9 94 5 0.0 3 0 0 1 4 95 1 0.0 3 0 0 0 1 97 6 0.0 3 0 0 0 6 98 29 0.0 3 0 0 0 29 99 5548 0.0 3 0 0 40 5508 100 731 0.0 3 0 0 6 725 101 11 0.0 3 0 0 0 11 102 469 0.0 3 0 0 20 449 103 107 0.0 3 0 0 2 105 104 1 0.0 3 0 0 0 1 105 5 0.0 3 0 0 0 5 106 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 1 1 120 1 0.0 3 0 0 0 1 122 8 0.0 3 0 0 0 8 123 9 0.0 3 0 0 1 8 124 7 0.0 3 0 0 1 6 125 41 0.0 3 0 0 2 39 126 4 0.0 3 0 0 1 3 127 4 0.0 3 0 0 0 4 130 4 0.0 3 0 0 1 3 131 4 0.0 3 0 0 0 4 132 2 0.0 3 0 0 0 2 133 2 0.0 3 0 0 0 2 134 5 0.0 3 0 0 0 5 135 3 0.0 3 0 0 0 3 137 3 0.0 3 0 0 0 3 141 7 0.0 3 0 0 0 7 144 8 0.0 3 3 2 3 145 3 0.0 3 0 0 0 3 146 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 4 0.0 3 0 0 1 3 150 113 0.0 3 0 0 4 109 151 33 0.0 3 0 0 1 32 152 8 0.0 3 0 0 0 8 153 19 0.0 3 0 0 0 19 154 5 0.0 3 0 0 0 5 155 1 0.0 3 0 0 0 1 156 5 0.0 3 0 0 0 5 158 5 0.0 3 0 0 0 5 159 29 0.0 3 0 0 0 29 160 20 0.0 3 0 0 0 20 161 2 0.0 3 0 0 0 2 162 4 0.0 3 0 0 0 4 163 1 0.0 3 0 0 0 1 164 6 0.0 3 0 0 0 6 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 170 2 0.0 3 0 0 0 2 171 1 0.0 3 0 0 0 1 172 3 0.0 3 0 0 0 3 173 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 176 3 0.0 3 0 0 0 3 177 5 0.0 3 0 0 1 4 178 4 0.0 3 0 0 0 4 179 2 0.0 3 0 0 0 2 181 1 0.0 3 0 0 0 1 183 3 0.0 3 0 0 1 2 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 187 3 0.0 3 0 0 0 3 188 7 0.0 3 0 0 1 6 189 1 0.0 3 0 0 0 1 190 5 0.0 3 0 0 0 5 191 30 0.0 3 0 0 1 29 192 1 0.0 3 0 0 0 1 193 2 0.0 3 0 0 0 2 194 4 0.0 3 0 0 0 4 195 2 0.0 3 0 0 0 2 196 1 0.0 3 0 0 0 1 199 9 0.0 3 0 0 0 9 200 272 0.0 3 0 0 2 270 201 68 0.0 3 0 0 5 63 202 20 0.0 3 0 0 2 18 203 5 0.0 3 0 0 0 5 204 6 0.0 3 0 0 0 6 205 6 0.0 3 0 0 0 6 206 3 0.0 3 0 0 0 3 207 2 0.0 3 0 0 0 2 208 4 0.0 3 0 0 0 4 209 8 0.0 3 0 0 0 8 211 2 0.0 3 0 0 1 1 212 1 0.0 3 0 0 0 1 213 3 0.0 3 0 0 0 3 214 1 0.0 3 0 0 0 1 215 2 0.0 3 0 0 0 2 216 6 0.0 3 0 0 1 5 217 144 0.0 3 0 0 5 139 218 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 221 4 0.0 3 0 0 0 4 222 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 226 2 0.0 3 0 0 0 2 233 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 239 3 0.0 3 0 0 0 3 240 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 7 0.0 3 0 0 0 7 281 5 0.0 3 0 0 0 5 282 6 0.0 3 0 0 0 6 283 2 0.0 3 0 0 0 2 285 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 287 2 0.0 3 0 0 0 2 289 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.45 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 100,446 Reads with adapters: 405 (0.4%) Reads written (passing filters): 100,446 (100.0%) Total basepairs processed: 26,525,832 bp Total written (filtered): 26,524,563 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 405 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 56.5% C: 17.5% G: 20.0% T: 5.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 367 1569.5 0 367 4 28 392.4 0 28 5 9 98.1 0 9 11 1 0.0 1 0 1