This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCGGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.68 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 468,916 (19.0%) Reads that were too short: 29,704 (1.2%) Reads written (passing filters): 439,212 (17.8%) Total basepairs processed: 740,966,583 bp Total written (filtered): 104,572,769 bp (14.1%) === Adapter 1 === Sequence: TTCGGA; Type: regular 5'; Length: 6; Trimmed: 468916 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 865 601.0 0 865 7 3282 601.0 0 3282 8 88365 601.0 0 88365 9 95252 601.0 0 95252 10 102 601.0 0 102 11 71 601.0 0 71 12 77 601.0 0 77 13 30 601.0 0 30 14 29 601.0 0 29 15 17 601.0 0 17 16 21 601.0 0 21 17 27 601.0 0 27 18 20 601.0 0 20 19 27 601.0 0 27 20 25 601.0 0 25 21 27 601.0 0 27 22 36 601.0 0 36 23 54 601.0 0 54 24 55 601.0 0 55 25 38 601.0 0 38 26 42 601.0 0 42 27 56 601.0 0 56 28 31 601.0 0 31 29 33 601.0 0 33 30 31 601.0 0 31 31 32 601.0 0 32 32 49 601.0 0 49 33 35 601.0 0 35 34 21 601.0 0 21 35 32 601.0 0 32 36 33 601.0 0 33 37 20 601.0 0 20 38 52 601.0 0 52 39 94 601.0 0 94 40 63 601.0 0 63 41 69 601.0 0 69 42 53 601.0 0 53 43 42 601.0 0 42 44 26 601.0 0 26 45 35 601.0 0 35 46 33 601.0 0 33 47 55 601.0 0 55 48 66 601.0 0 66 49 80 601.0 0 80 50 53 601.0 0 53 51 40 601.0 0 40 52 68 601.0 0 68 53 54 601.0 0 54 54 95 601.0 0 95 55 25 601.0 0 25 56 48 601.0 0 48 57 77 601.0 0 77 58 44 601.0 0 44 59 52 601.0 0 52 60 58 601.0 0 58 61 42 601.0 0 42 62 55 601.0 0 55 63 64 601.0 0 64 64 35 601.0 0 35 65 58 601.0 0 58 66 102 601.0 0 102 67 48 601.0 0 48 68 177 601.0 0 177 69 85 601.0 0 85 70 67 601.0 0 67 71 51 601.0 0 51 72 157 601.0 0 157 73 86 601.0 0 86 74 119 601.0 0 119 75 153 601.0 0 153 76 190 601.0 0 190 77 326 601.0 0 326 78 663 601.0 0 663 79 1233 601.0 0 1233 80 344 601.0 0 344 81 418 601.0 0 418 82 779 601.0 0 779 83 417 601.0 0 417 84 325 601.0 0 325 85 656 601.0 0 656 86 712 601.0 0 712 87 588 601.0 0 588 88 724 601.0 0 724 89 1174 601.0 0 1174 90 1726 601.0 0 1726 91 3165 601.0 0 3165 92 111546 601.0 0 111546 93 45143 601.0 0 45143 94 47831 601.0 0 47831 95 207 601.0 0 207 96 27 601.0 0 27 97 38 601.0 0 38 98 38 601.0 0 38 99 25 601.0 0 25 100 25 601.0 0 25 101 27 601.0 0 27 102 20 601.0 0 20 103 19 601.0 0 19 104 17 601.0 0 17 105 19 601.0 0 19 106 17 601.0 0 17 107 17 601.0 0 17 108 28 601.0 0 28 109 25 601.0 0 25 110 143 601.0 0 143 111 35 601.0 0 35 112 71 601.0 0 71 113 25 601.0 0 25 114 37 601.0 0 37 115 28 601.0 0 28 116 20 601.0 0 20 117 49 601.0 0 49 118 33 601.0 0 33 119 18 601.0 0 18 120 22 601.0 0 22 121 8 601.0 0 8 122 20 601.0 0 20 123 17 601.0 0 17 124 19 601.0 0 19 125 54 601.0 0 54 126 23 601.0 0 23 127 25 601.0 0 25 128 35 601.0 0 35 129 34 601.0 0 34 130 37 601.0 0 37 131 148 601.0 0 148 132 34 601.0 0 34 133 25 601.0 0 25 134 31 601.0 0 31 135 34 601.0 0 34 136 25 601.0 0 25 137 35 601.0 0 35 138 41 601.0 0 41 139 31 601.0 0 31 140 41 601.0 0 41 141 13 601.0 0 13 142 25 601.0 0 25 143 30 601.0 0 30 144 26 601.0 0 26 145 29 601.0 0 29 146 37 601.0 0 37 147 21 601.0 0 21 148 29 601.0 0 29 149 31 601.0 0 31 150 18 601.0 0 18 151 38 601.0 0 38 152 31 601.0 0 31 153 23 601.0 0 23 154 24 601.0 0 24 155 48 601.0 0 48 156 28 601.0 0 28 157 35 601.0 0 35 158 41 601.0 0 41 159 40 601.0 0 40 160 28 601.0 0 28 161 35 601.0 0 35 162 25 601.0 0 25 163 30 601.0 0 30 164 19 601.0 0 19 165 23 601.0 0 23 166 23 601.0 0 23 167 18 601.0 0 18 168 24 601.0 0 24 169 27 601.0 0 27 170 24 601.0 0 24 171 32 601.0 0 32 172 37 601.0 0 37 173 22 601.0 0 22 174 38 601.0 0 38 175 47 601.0 0 47 176 43 601.0 0 43 177 92 601.0 0 92 178 275 601.0 0 275 179 339 601.0 0 339 180 271 601.0 0 271 181 58 601.0 0 58 182 38 601.0 0 38 183 50 601.0 0 50 184 62 601.0 0 62 185 90 601.0 0 90 186 137 601.0 0 137 187 320 601.0 0 320 188 524 601.0 0 524 189 117 601.0 0 117 190 148 601.0 0 148 191 333 601.0 0 333 192 142 601.0 0 142 193 128 601.0 0 128 194 232 601.0 0 232 195 186 601.0 0 186 196 180 601.0 0 180 197 177 601.0 0 177 198 301 601.0 0 301 199 345 601.0 0 345 200 692 601.0 0 692 201 21790 601.0 0 21790 202 6491 601.0 0 6491 203 8889 601.0 0 8889 204 382 601.0 0 382 205 457 601.0 0 457 206 860 601.0 0 860 207 928 601.0 0 928 208 213 601.0 0 213 209 122 601.0 0 122 210 70 601.0 0 70 211 91 601.0 0 91 212 160 601.0 0 160 213 302 601.0 0 302 214 50 601.0 0 50 215 37 601.0 0 37 216 124 601.0 0 124 217 96 601.0 0 96 218 64 601.0 0 64 219 82 601.0 0 82 220 58 601.0 0 58 221 158 601.0 0 158 222 183 601.0 0 183 223 184 601.0 0 184 224 62 601.0 0 62 225 406 601.0 0 406 226 569 601.0 0 569 227 1150 601.0 0 1150 228 407 601.0 0 407 229 537 601.0 0 537 230 31 601.0 0 31 231 21 601.0 0 21 232 16 601.0 0 16 233 20 601.0 0 20 234 23 601.0 0 23 235 23 601.0 0 23 236 43 601.0 0 43 237 59 601.0 0 59 238 46 601.0 0 46 239 19 601.0 0 19 240 16 601.0 0 16 241 20 601.0 0 20 242 24 601.0 0 24 243 31 601.0 0 31 244 28 601.0 0 28 245 35 601.0 0 35 246 40 601.0 0 40 247 31 601.0 0 31 248 31 601.0 0 31 249 28 601.0 0 28 250 42 601.0 0 42 251 21 601.0 0 21 252 22 601.0 0 22 253 33 601.0 0 33 254 26 601.0 0 26 255 35 601.0 0 35 256 41 601.0 0 41 257 40 601.0 0 40 258 50 601.0 0 50 259 246 601.0 0 246 260 541 601.0 0 541 261 858 601.0 0 858 262 740 601.0 0 740 263 267 601.0 0 267 264 298 601.0 0 298 265 34 601.0 0 34 266 25 601.0 0 25 267 29 601.0 0 29 268 26 601.0 0 26 269 34 601.0 0 34 270 87 601.0 0 87 271 93 601.0 0 93 272 86 601.0 0 86 273 57 601.0 0 57 274 36 601.0 0 36 275 28 601.0 0 28 276 165 601.0 0 165 277 79 601.0 0 79 278 39 601.0 0 39 279 23 601.0 0 23 280 97 601.0 0 97 281 108 601.0 0 108 282 97 601.0 0 97 283 73 601.0 0 73 284 51 601.0 0 51 285 69 601.0 0 69 286 156 601.0 0 156 287 38 601.0 0 38 288 60 601.0 0 60 289 31 601.0 0 31 290 31 601.0 0 31 291 25 601.0 0 25 292 30 601.0 0 30 293 48 601.0 0 48 294 34 601.0 0 34 295 35 601.0 0 35 296 70 601.0 0 70 297 470 601.0 0 470 298 97 601.0 0 97 299 216 601.0 0 216 300 29 601.0 0 29 301 21 601.0 0 21 Finished in 19.05 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 439,212 Reads with adapters: 100,971 (23.0%) Reads that were too short: 59 (0.0%) Reads written (passing filters): 100,912 (23.0%) Total basepairs processed: 104,572,769 bp Total written (filtered): 27,381,343 bp (26.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 100971 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 2 2 18 47 0.0 3 0 0 43 4 19 1598 0.0 3 0 1569 27 1 1 20 98311 0.0 4 96734 1522 47 6 2 21 190 0.0 4 24 158 6 1 1 22 13 0.0 4 0 0 13 23 58 0.0 4 1 0 0 54 3 24 5 0.0 4 0 0 0 1 4 25 2 0.0 4 1 0 0 1 27 1 0.0 4 0 1 28 1 0.0 4 1 31 2 0.0 4 2 38 1 0.0 4 0 1 43 1 0.0 4 0 0 0 0 1 44 1 0.0 4 1 58 1 0.0 4 1 60 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 65 1 0.0 4 1 67 2 0.0 4 0 0 0 0 2 68 14 0.0 4 0 0 0 0 14 94 2 0.0 4 0 0 0 0 2 117 1 0.0 4 0 0 0 0 1 122 2 0.0 4 0 0 0 0 2 123 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 5 0.0 4 0 0 0 0 5 127 635 0.0 4 0 0 0 0 635 128 2 0.0 4 0 0 0 0 2 129 1 0.0 4 1 130 1 0.0 4 0 0 0 0 1 135 12 0.0 4 0 0 0 0 12 136 2 0.0 4 1 0 0 0 1 139 1 0.0 4 1 148 2 0.0 4 0 0 0 0 2 149 6 0.0 4 0 0 0 0 6 150 5 0.0 4 0 0 0 0 5 151 8 0.0 4 1 0 0 0 7 172 3 0.0 4 0 0 0 0 3 173 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 176 1 0.0 4 1 189 1 0.0 4 0 0 0 0 1 198 2 0.0 4 0 0 0 0 2 208 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 0 4 229 4 0.0 4 0 0 0 0 4 250 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 1 280 1 0.0 4 1 284 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.62 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 100,912 Reads with adapters: 14,102 (14.0%) Reads written (passing filters): 100,912 (100.0%) Total basepairs processed: 27,381,343 bp Total written (filtered): 27,244,722 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 14102 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.8% C: 2.7% G: 95.2% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 30 1576.8 0 30 4 20 394.2 0 20 5 15 98.5 0 15 6 3 24.6 0 3 7 8 6.2 0 8 9 6345 0.4 0 168 6177 10 6671 0.1 1 261 6410 11 983 0.0 1 4 979 12 11 0.0 1 0 11 13 5 0.0 1 0 5 14 1 0.0 1 0 1 35 1 0.0 1 1 37 1 0.0 1 0 1 91 1 0.0 1 1 150 2 0.0 1 0 2 169 1 0.0 1 0 1 170 1 0.0 1 1 196 1 0.0 1 1 208 1 0.0 1 1 257 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.