This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAGAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.46 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 278,118 (11.4%) Reads that were too short: 14,261 (0.6%) Reads written (passing filters): 263,857 (10.8%) Total basepairs processed: 735,190,694 bp Total written (filtered): 58,904,103 bp (8.0%) === Adapter 1 === Sequence: AGAGAC; Type: regular 5'; Length: 6; Trimmed: 278118 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 323 596.3 0 323 7 12998 596.3 0 12998 8 7370 596.3 0 7370 9 18 596.3 0 18 10 104 596.3 0 104 11 26 596.3 0 26 12 10 596.3 0 10 13 21 596.3 0 21 14 12 596.3 0 12 15 13 596.3 0 13 16 7 596.3 0 7 17 7 596.3 0 7 18 14 596.3 0 14 19 17 596.3 0 17 20 13 596.3 0 13 21 43 596.3 0 43 22 32 596.3 0 32 23 24 596.3 0 24 24 7 596.3 0 7 25 10 596.3 0 10 26 10 596.3 0 10 27 17 596.3 0 17 28 15 596.3 0 15 29 17 596.3 0 17 30 16 596.3 0 16 31 16 596.3 0 16 32 11 596.3 0 11 33 44 596.3 0 44 34 11 596.3 0 11 35 36 596.3 0 36 36 18 596.3 0 18 37 14 596.3 0 14 38 8 596.3 0 8 39 28 596.3 0 28 40 29 596.3 0 29 41 16 596.3 0 16 42 74 596.3 0 74 43 49 596.3 0 49 44 55 596.3 0 55 45 42 596.3 0 42 46 33 596.3 0 33 47 39 596.3 0 39 48 22 596.3 0 22 49 88 596.3 0 88 50 39 596.3 0 39 51 62 596.3 0 62 52 547 596.3 0 547 53 118 596.3 0 118 54 133 596.3 0 133 55 200 596.3 0 200 56 18 596.3 0 18 57 24 596.3 0 24 58 15 596.3 0 15 59 70 596.3 0 70 60 79 596.3 0 79 61 286 596.3 0 286 62 739 596.3 0 739 63 160 596.3 0 160 64 385 596.3 0 385 65 754 596.3 0 754 66 1614 596.3 0 1614 67 2291 596.3 0 2291 68 194 596.3 0 194 69 247 596.3 0 247 70 88 596.3 0 88 71 151 596.3 0 151 72 419 596.3 0 419 73 489 596.3 0 489 74 817 596.3 0 817 75 1096 596.3 0 1096 76 1156 596.3 0 1156 77 2522 596.3 0 2522 78 5875 596.3 0 5875 79 84483 596.3 0 84483 80 35744 596.3 0 35744 81 83249 596.3 0 83249 82 590 596.3 0 590 83 295 596.3 0 295 84 41 596.3 0 41 85 25 596.3 0 25 86 25 596.3 0 25 87 48 596.3 0 48 88 29 596.3 0 29 89 20 596.3 0 20 90 43 596.3 0 43 91 10 596.3 0 10 92 39 596.3 0 39 93 24 596.3 0 24 94 16 596.3 0 16 95 37 596.3 0 37 96 27 596.3 0 27 97 17 596.3 0 17 98 11 596.3 0 11 99 13 596.3 0 13 100 13 596.3 0 13 101 12 596.3 0 12 102 26 596.3 0 26 103 24 596.3 0 24 104 20 596.3 0 20 105 17 596.3 0 17 106 26 596.3 0 26 107 23 596.3 0 23 108 22 596.3 0 22 109 11 596.3 0 11 110 16 596.3 0 16 111 26 596.3 0 26 112 38 596.3 0 38 113 37 596.3 0 37 114 55 596.3 0 55 115 61 596.3 0 61 116 478 596.3 0 478 117 476 596.3 0 476 118 700 596.3 0 700 119 114 596.3 0 114 120 58 596.3 0 58 121 77 596.3 0 77 122 84 596.3 0 84 123 119 596.3 0 119 124 137 596.3 0 137 125 201 596.3 0 201 126 188 596.3 0 188 127 58 596.3 0 58 128 50 596.3 0 50 129 50 596.3 0 50 130 268 596.3 0 268 131 568 596.3 0 568 132 550 596.3 0 550 133 92 596.3 0 92 134 50 596.3 0 50 135 54 596.3 0 54 136 45 596.3 0 45 137 97 596.3 0 97 138 70 596.3 0 70 139 116 596.3 0 116 140 269 596.3 0 269 141 578 596.3 0 578 142 158 596.3 0 158 143 120 596.3 0 120 144 96 596.3 0 96 145 207 596.3 0 207 146 186 596.3 0 186 147 199 596.3 0 199 148 146 596.3 0 146 149 244 596.3 0 244 150 2104 596.3 0 2104 151 1773 596.3 0 1773 152 1492 596.3 0 1492 153 81 596.3 0 81 154 59 596.3 0 59 155 62 596.3 0 62 156 38 596.3 0 38 157 49 596.3 0 49 158 64 596.3 0 64 159 42 596.3 0 42 160 35 596.3 0 35 161 37 596.3 0 37 162 48 596.3 0 48 163 49 596.3 0 49 164 35 596.3 0 35 165 74 596.3 0 74 166 88 596.3 0 88 167 132 596.3 0 132 168 89 596.3 0 89 169 101 596.3 0 101 170 122 596.3 0 122 171 325 596.3 0 325 172 370 596.3 0 370 173 190 596.3 0 190 174 85 596.3 0 85 175 40 596.3 0 40 176 49 596.3 0 49 177 42 596.3 0 42 178 42 596.3 0 42 179 46 596.3 0 46 180 44 596.3 0 44 181 38 596.3 0 38 182 57 596.3 0 57 183 55 596.3 0 55 184 80 596.3 0 80 185 87 596.3 0 87 186 208 596.3 0 208 187 113 596.3 0 113 188 107 596.3 0 107 189 175 596.3 0 175 190 60 596.3 0 60 191 50 596.3 0 50 192 52 596.3 0 52 193 62 596.3 0 62 194 63 596.3 0 63 195 50 596.3 0 50 196 43 596.3 0 43 197 48 596.3 0 48 198 58 596.3 0 58 199 64 596.3 0 64 200 59 596.3 0 59 201 40 596.3 0 40 202 45 596.3 0 45 203 56 596.3 0 56 204 47 596.3 0 47 205 66 596.3 0 66 206 56 596.3 0 56 207 57 596.3 0 57 208 55 596.3 0 55 209 108 596.3 0 108 210 90 596.3 0 90 211 64 596.3 0 64 212 64 596.3 0 64 213 80 596.3 0 80 214 62 596.3 0 62 215 69 596.3 0 69 216 68 596.3 0 68 217 98 596.3 0 98 218 368 596.3 0 368 219 191 596.3 0 191 220 65 596.3 0 65 221 104 596.3 0 104 222 130 596.3 0 130 223 168 596.3 0 168 224 130 596.3 0 130 225 207 596.3 0 207 226 857 596.3 0 857 227 1088 596.3 0 1088 228 862 596.3 0 862 229 249 596.3 0 249 230 123 596.3 0 123 231 85 596.3 0 85 232 64 596.3 0 64 233 67 596.3 0 67 234 68 596.3 0 68 235 36 596.3 0 36 236 51 596.3 0 51 237 45 596.3 0 45 238 60 596.3 0 60 239 79 596.3 0 79 240 71 596.3 0 71 241 106 596.3 0 106 242 79 596.3 0 79 243 86 596.3 0 86 244 60 596.3 0 60 245 83 596.3 0 83 246 131 596.3 0 131 247 199 596.3 0 199 248 163 596.3 0 163 249 121 596.3 0 121 250 102 596.3 0 102 251 119 596.3 0 119 252 115 596.3 0 115 253 105 596.3 0 105 254 77 596.3 0 77 255 116 596.3 0 116 256 113 596.3 0 113 257 82 596.3 0 82 258 74 596.3 0 74 259 91 596.3 0 91 260 101 596.3 0 101 261 114 596.3 0 114 262 98 596.3 0 98 263 104 596.3 0 104 264 105 596.3 0 105 265 110 596.3 0 110 266 110 596.3 0 110 267 78 596.3 0 78 268 112 596.3 0 112 269 83 596.3 0 83 270 64 596.3 0 64 271 64 596.3 0 64 272 89 596.3 0 89 273 98 596.3 0 98 274 233 596.3 0 233 275 258 596.3 0 258 276 254 596.3 0 254 277 210 596.3 0 210 278 115 596.3 0 115 279 181 596.3 0 181 280 179 596.3 0 179 281 301 596.3 0 301 282 101 596.3 0 101 283 118 596.3 0 118 284 87 596.3 0 87 285 221 596.3 0 221 286 96 596.3 0 96 287 162 596.3 0 162 288 121 596.3 0 121 289 118 596.3 0 118 290 169 596.3 0 169 291 179 596.3 0 179 292 227 596.3 0 227 293 162 596.3 0 162 294 118 596.3 0 118 295 89 596.3 0 89 296 96 596.3 0 96 297 94 596.3 0 94 298 168 596.3 0 168 299 217 596.3 0 217 300 250 596.3 0 250 301 132 596.3 0 132 Finished in 10.01 s (38 us/read; 1.58 M reads/minute). === Summary === Total reads processed: 263,857 Reads with adapters: 11,911 (4.5%) Reads that were too short: 509 (0.2%) Reads written (passing filters): 11,402 (4.3%) Total basepairs processed: 58,904,103 bp Total written (filtered): 3,075,808 bp (5.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 11911 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 6 0.0 3 0 0 5 1 16 117 0.0 3 0 93 16 8 17 10627 0.0 3 9967 536 106 18 18 35 0.0 3 5 23 2 5 19 45 0.0 3 2 2 27 14 20 40 0.0 3 6 1 0 33 22 1 0.0 3 0 0 0 1 23 10 0.0 3 0 0 2 8 24 1 0.0 3 0 0 0 1 25 2 0.0 3 0 0 1 1 26 4 0.0 3 0 0 0 4 28 1 0.0 3 0 0 0 1 29 5 0.0 3 0 0 0 5 30 1 0.0 3 0 0 0 1 31 5 0.0 3 0 0 1 4 34 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 41 147 0.0 3 0 0 1 146 43 2 0.0 3 0 0 0 2 44 26 0.0 3 0 0 1 25 46 2 0.0 3 0 0 0 2 47 11 0.0 3 0 0 0 11 48 3 0.0 3 0 1 0 2 49 1 0.0 3 0 0 0 1 50 9 0.0 3 0 0 0 9 51 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 6 0.0 3 0 0 0 6 56 4 0.0 3 0 0 0 4 57 11 0.0 3 0 0 0 11 58 5 0.0 3 0 0 0 5 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 62 2 0.0 3 0 1 0 1 63 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 66 3 0.0 3 0 0 0 3 67 5 0.0 3 0 0 0 5 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 3 0.0 3 0 0 0 3 72 1 0.0 3 0 0 0 1 73 4 0.0 3 0 0 0 4 75 14 0.0 3 0 0 0 14 76 18 0.0 3 0 0 0 18 78 3 0.0 3 0 0 0 3 79 4 0.0 3 0 0 0 4 80 5 0.0 3 0 0 0 5 81 1 0.0 3 0 0 0 1 84 8 0.0 3 0 0 0 8 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 2 0.0 3 0 0 0 2 89 2 0.0 3 0 0 0 2 90 2 0.0 3 0 0 0 2 92 9 0.0 3 0 0 0 9 93 1 0.0 3 0 0 0 1 95 3 0.0 3 0 0 0 3 97 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 10 0.0 3 0 0 0 10 102 2 0.0 3 0 0 0 2 103 2 0.0 3 0 0 0 2 104 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 106 68 0.0 3 0 0 0 68 107 15 0.0 3 0 0 0 15 108 7 0.0 3 0 0 0 7 109 27 0.0 3 0 0 1 26 110 8 0.0 3 0 0 1 7 111 10 0.0 3 0 0 0 10 112 4 0.0 3 0 0 0 4 113 3 0.0 3 1 0 0 2 114 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 119 3 0.0 3 0 0 0 3 120 5 0.0 3 0 0 0 5 121 1 0.0 3 0 0 1 122 5 0.0 3 0 0 0 5 123 4 0.0 3 0 0 0 4 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 3 0.0 3 0 0 0 3 127 5 0.0 3 0 0 0 5 128 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 130 3 0.0 3 0 0 0 3 132 5 0.0 3 0 0 0 5 133 16 0.0 3 0 0 0 16 134 8 0.0 3 0 0 0 8 135 5 0.0 3 0 0 0 5 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 140 2 0.0 3 0 0 0 2 141 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 144 10 0.0 3 0 0 0 10 145 2 0.0 3 0 0 0 2 146 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 67 0.0 3 0 0 1 66 150 5 0.0 3 0 0 0 5 153 1 0.0 3 0 0 0 1 157 7 0.0 3 0 0 0 7 158 2 0.0 3 0 0 0 2 159 3 0.0 3 0 0 0 3 161 1 0.0 3 0 0 0 1 162 3 0.0 3 0 0 0 3 163 2 0.0 3 0 0 0 2 165 2 0.0 3 0 0 0 2 166 8 0.0 3 0 0 0 8 167 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 176 2 0.0 3 0 0 0 2 178 4 0.0 3 0 0 1 3 181 1 0.0 3 0 0 1 182 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 1 186 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 196 2 0.0 3 0 0 0 2 197 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 207 45 0.0 3 0 0 0 45 208 24 0.0 3 0 0 0 24 209 70 0.0 3 0 0 2 68 210 66 0.0 3 0 0 4 62 211 9 0.0 3 0 0 0 9 213 2 0.0 3 0 0 0 2 215 1 0.0 3 0 0 0 1 216 9 0.0 3 0 0 0 9 217 5 0.0 3 0 0 0 5 218 8 0.0 3 0 0 0 8 219 4 0.0 3 0 0 0 4 220 8 0.0 3 0 0 0 8 221 5 0.0 3 0 0 0 5 222 3 0.0 3 0 0 0 3 223 3 0.0 3 0 0 0 3 224 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 1 227 3 0.0 3 0 0 0 3 228 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 234 2 0.0 3 0 0 0 2 239 3 0.0 3 0 0 1 2 240 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 251 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 4 0.0 3 0 0 1 3 282 2 0.0 3 0 0 0 2 287 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.39 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 11,402 Reads with adapters: 250 (2.2%) Reads written (passing filters): 11,402 (100.0%) Total basepairs processed: 3,075,808 bp Total written (filtered): 3,075,052 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 250 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 39.6% C: 48.0% G: 10.4% T: 2.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 246 178.2 0 246 4 2 44.5 0 2 5 2 11.1 0 2