This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGAATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.00 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 143,180 (5.8%) Reads that were too short: 6,575 (0.3%) Reads written (passing filters): 136,605 (5.5%) Total basepairs processed: 740,966,583 bp Total written (filtered): 37,799,084 bp (5.1%) === Adapter 1 === Sequence: CGAATC; Type: regular 5'; Length: 6; Trimmed: 143180 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 5840 601.0 0 5840 7 39845 601.0 0 39845 8 2438 601.0 0 2438 9 59544 601.0 0 59544 10 59 601.0 0 59 11 28 601.0 0 28 12 15 601.0 0 15 13 16 601.0 0 16 14 15 601.0 0 15 15 18 601.0 0 18 16 12 601.0 0 12 17 17 601.0 0 17 18 14 601.0 0 14 19 21 601.0 0 21 20 27 601.0 0 27 21 34 601.0 0 34 22 40 601.0 0 40 23 40 601.0 0 40 24 43 601.0 0 43 25 32 601.0 0 32 26 20 601.0 0 20 27 69 601.0 0 69 28 110 601.0 0 110 29 124 601.0 0 124 30 922 601.0 0 922 31 14 601.0 0 14 32 17 601.0 0 17 33 203 601.0 0 203 34 143 601.0 0 143 35 100 601.0 0 100 36 25 601.0 0 25 37 38 601.0 0 38 38 23 601.0 0 23 39 30 601.0 0 30 40 24 601.0 0 24 41 16 601.0 0 16 42 35 601.0 0 35 43 37 601.0 0 37 44 42 601.0 0 42 45 21 601.0 0 21 46 40 601.0 0 40 47 48 601.0 0 48 48 46 601.0 0 46 49 63 601.0 0 63 50 102 601.0 0 102 51 125 601.0 0 125 52 180 601.0 0 180 53 276 601.0 0 276 54 2337 601.0 0 2337 55 3221 601.0 0 3221 56 7422 601.0 0 7422 57 32 601.0 0 32 58 30 601.0 0 30 59 32 601.0 0 32 60 35 601.0 0 35 61 11 601.0 0 11 62 25 601.0 0 25 63 21 601.0 0 21 64 22 601.0 0 22 65 9 601.0 0 9 66 16 601.0 0 16 67 32 601.0 0 32 68 53 601.0 0 53 69 29 601.0 0 29 70 20 601.0 0 20 71 10 601.0 0 10 72 12 601.0 0 12 73 18 601.0 0 18 74 21 601.0 0 21 75 23 601.0 0 23 76 21 601.0 0 21 77 21 601.0 0 21 78 13 601.0 0 13 79 19 601.0 0 19 80 21 601.0 0 21 81 52 601.0 0 52 82 26 601.0 0 26 83 28 601.0 0 28 84 31 601.0 0 31 85 23 601.0 0 23 86 20 601.0 0 20 87 34 601.0 0 34 88 22 601.0 0 22 89 27 601.0 0 27 90 24 601.0 0 24 91 28 601.0 0 28 92 44 601.0 0 44 93 70 601.0 0 70 94 1826 601.0 0 1826 95 702 601.0 0 702 96 329 601.0 0 329 97 12 601.0 0 12 98 17 601.0 0 17 99 20 601.0 0 20 100 13 601.0 0 13 101 18 601.0 0 18 102 27 601.0 0 27 103 21 601.0 0 21 104 34 601.0 0 34 105 19 601.0 0 19 106 17 601.0 0 17 107 29 601.0 0 29 108 21 601.0 0 21 109 150 601.0 0 150 110 461 601.0 0 461 111 702 601.0 0 702 112 18 601.0 0 18 113 12 601.0 0 12 114 13 601.0 0 13 115 19 601.0 0 19 116 25 601.0 0 25 117 28 601.0 0 28 118 34 601.0 0 34 119 80 601.0 0 80 120 174 601.0 0 174 121 1079 601.0 0 1079 122 282 601.0 0 282 123 133 601.0 0 133 124 19 601.0 0 19 125 31 601.0 0 31 126 28 601.0 0 28 127 23 601.0 0 23 128 38 601.0 0 38 129 17 601.0 0 17 130 16 601.0 0 16 131 21 601.0 0 21 132 22 601.0 0 22 133 35 601.0 0 35 134 17 601.0 0 17 135 40 601.0 0 40 136 72 601.0 0 72 137 63 601.0 0 63 138 42 601.0 0 42 139 111 601.0 0 111 140 165 601.0 0 165 141 182 601.0 0 182 142 46 601.0 0 46 143 31 601.0 0 31 144 24 601.0 0 24 145 48 601.0 0 48 146 55 601.0 0 55 147 64 601.0 0 64 148 167 601.0 0 167 149 142 601.0 0 142 150 53 601.0 0 53 151 53 601.0 0 53 152 38 601.0 0 38 153 35 601.0 0 35 154 53 601.0 0 53 155 29 601.0 0 29 156 31 601.0 0 31 157 31 601.0 0 31 158 26 601.0 0 26 159 43 601.0 0 43 160 171 601.0 0 171 161 436 601.0 0 436 162 162 601.0 0 162 163 78 601.0 0 78 164 29 601.0 0 29 165 117 601.0 0 117 166 208 601.0 0 208 167 223 601.0 0 223 168 53 601.0 0 53 169 30 601.0 0 30 170 27 601.0 0 27 171 21 601.0 0 21 172 18 601.0 0 18 173 48 601.0 0 48 174 51 601.0 0 51 175 100 601.0 0 100 176 102 601.0 0 102 177 276 601.0 0 276 178 186 601.0 0 186 179 27 601.0 0 27 180 109 601.0 0 109 181 134 601.0 0 134 182 319 601.0 0 319 183 39 601.0 0 39 184 21 601.0 0 21 185 27 601.0 0 27 186 31 601.0 0 31 187 22 601.0 0 22 188 42 601.0 0 42 189 10 601.0 0 10 190 20 601.0 0 20 191 24 601.0 0 24 192 21 601.0 0 21 193 36 601.0 0 36 194 44 601.0 0 44 195 20 601.0 0 20 196 26 601.0 0 26 197 17 601.0 0 17 198 27 601.0 0 27 199 35 601.0 0 35 200 23 601.0 0 23 201 23 601.0 0 23 202 30 601.0 0 30 203 46 601.0 0 46 204 99 601.0 0 99 205 65 601.0 0 65 206 33 601.0 0 33 207 30 601.0 0 30 208 44 601.0 0 44 209 85 601.0 0 85 210 42 601.0 0 42 211 26 601.0 0 26 212 23 601.0 0 23 213 28 601.0 0 28 214 31 601.0 0 31 215 33 601.0 0 33 216 24 601.0 0 24 217 27 601.0 0 27 218 30 601.0 0 30 219 42 601.0 0 42 220 44 601.0 0 44 221 49 601.0 0 49 222 53 601.0 0 53 223 77 601.0 0 77 224 59 601.0 0 59 225 42 601.0 0 42 226 54 601.0 0 54 227 44 601.0 0 44 228 43 601.0 0 43 229 79 601.0 0 79 230 75 601.0 0 75 231 51 601.0 0 51 232 28 601.0 0 28 233 24 601.0 0 24 234 37 601.0 0 37 235 17 601.0 0 17 236 27 601.0 0 27 237 28 601.0 0 28 238 36 601.0 0 36 239 40 601.0 0 40 240 39 601.0 0 39 241 31 601.0 0 31 242 29 601.0 0 29 243 28 601.0 0 28 244 40 601.0 0 40 245 18 601.0 0 18 246 46 601.0 0 46 247 42 601.0 0 42 248 37 601.0 0 37 249 46 601.0 0 46 250 27 601.0 0 27 251 28 601.0 0 28 252 57 601.0 0 57 253 53 601.0 0 53 254 89 601.0 0 89 255 94 601.0 0 94 256 153 601.0 0 153 257 110 601.0 0 110 258 115 601.0 0 115 259 86 601.0 0 86 260 48 601.0 0 48 261 51 601.0 0 51 262 154 601.0 0 154 263 207 601.0 0 207 264 293 601.0 0 293 265 43 601.0 0 43 266 58 601.0 0 58 267 51 601.0 0 51 268 30 601.0 0 30 269 32 601.0 0 32 270 24 601.0 0 24 271 36 601.0 0 36 272 59 601.0 0 59 273 57 601.0 0 57 274 63 601.0 0 63 275 83 601.0 0 83 276 46 601.0 0 46 277 37 601.0 0 37 278 202 601.0 0 202 279 140 601.0 0 140 280 106 601.0 0 106 281 54 601.0 0 54 282 104 601.0 0 104 283 165 601.0 0 165 284 272 601.0 0 272 285 158 601.0 0 158 286 46 601.0 0 46 287 54 601.0 0 54 288 47 601.0 0 47 289 40 601.0 0 40 290 71 601.0 0 71 291 113 601.0 0 113 292 110 601.0 0 110 293 73 601.0 0 73 294 60 601.0 0 60 295 34 601.0 0 34 296 42 601.0 0 42 297 54 601.0 0 54 298 49 601.0 0 49 299 60 601.0 0 60 300 206 601.0 0 206 301 130 601.0 0 130 Finished in 6.33 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 136,605 Reads with adapters: 63,006 (46.1%) Reads that were too short: 154 (0.1%) Reads written (passing filters): 62,852 (46.0%) Total basepairs processed: 37,799,084 bp Total written (filtered): 17,204,501 bp (45.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 63006 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 9 0.0 2 0 0 0 9 15 40 0.0 3 0 0 24 16 16 552 0.0 3 0 456 66 30 17 61112 0.0 3 56159 3933 819 201 18 208 0.0 3 22 130 32 24 19 106 0.0 3 1 0 45 60 20 284 0.0 3 0 0 0 284 21 1 0.0 3 0 0 1 22 1 0.0 3 1 23 9 0.0 3 0 0 1 8 26 7 0.0 3 0 0 0 7 27 1 0.0 3 0 0 0 1 28 6 0.0 3 1 0 2 3 29 5 0.0 3 0 0 0 5 30 13 0.0 3 0 0 0 13 32 4 0.0 3 0 0 0 4 33 7 0.0 3 0 0 0 7 34 1 0.0 3 0 0 0 1 37 3 0.0 3 0 0 0 3 40 1 0.0 3 0 0 0 1 41 2 0.0 3 0 0 0 2 46 5 0.0 3 0 0 0 5 47 3 0.0 3 0 0 0 3 48 1 0.0 3 0 0 0 1 49 12 0.0 3 0 0 0 12 50 33 0.0 3 0 0 1 32 51 5 0.0 3 0 0 0 5 52 6 0.0 3 0 0 0 6 53 5 0.0 3 0 0 0 5 54 2 0.0 3 0 0 0 2 56 7 0.0 3 0 0 0 7 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 1 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 3 0.0 3 0 0 0 3 72 75 0.0 3 0 0 0 75 74 2 0.0 3 0 0 0 2 75 3 0.0 3 0 0 0 3 77 5 0.0 3 0 0 0 5 78 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 97 6 0.0 3 0 0 0 6 98 269 0.0 3 0 0 0 269 99 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 101 2 0.0 3 0 0 0 2 103 2 0.0 3 0 0 0 2 104 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 107 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 1 111 3 0.0 3 0 0 0 3 112 3 0.0 3 0 0 0 3 113 2 0.0 3 0 0 0 2 114 3 0.0 3 0 0 0 3 115 1 0.0 3 0 0 1 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 121 3 0.0 3 0 0 0 3 123 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 3 0.0 3 0 0 0 3 132 2 0.0 3 0 0 0 2 133 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 2 0.0 3 0 0 0 2 138 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 156 1 0.0 3 1 158 6 0.0 3 0 0 0 6 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 1 162 3 0.0 3 0 0 0 3 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 2 0.0 3 0 0 0 2 166 2 0.0 3 0 0 1 1 168 1 0.0 3 0 0 0 1 169 2 0.0 3 0 0 0 2 170 1 0.0 3 0 0 1 173 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 1 178 1 0.0 3 0 0 0 1 180 4 0.0 3 0 0 0 4 182 1 0.0 3 0 0 0 1 183 7 0.0 3 0 0 0 7 184 2 0.0 3 0 0 0 2 185 7 0.0 3 0 0 0 7 186 1 0.0 3 0 0 0 1 187 3 0.0 3 0 0 0 3 190 2 0.0 3 0 0 0 2 192 6 0.0 3 0 0 0 6 194 3 0.0 3 0 0 0 3 195 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 198 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 10 0.0 3 0 0 0 10 218 2 0.0 3 0 0 0 2 221 2 0.0 3 0 0 0 2 223 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 238 3 0.0 3 0 0 0 3 244 2 0.0 3 0 0 1 1 247 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 260 2 0.0 3 0 0 0 2 261 2 0.0 3 0 0 0 2 262 2 0.0 3 0 0 0 2 264 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 5 0.0 3 0 0 0 5 280 2 0.0 3 0 0 0 2 281 5 0.0 3 0 0 0 5 282 10 0.0 3 0 0 2 8 283 5 0.0 3 0 0 0 5 287 2 0.0 3 0 0 0 2 288 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.19 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 62,852 Reads with adapters: 1,054 (1.7%) Reads written (passing filters): 62,852 (100.0%) Total basepairs processed: 17,204,501 bp Total written (filtered): 17,201,287 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1054 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 60.7% C: 17.9% G: 16.6% T: 4.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1014 982.1 0 1014 4 33 245.5 0 33 5 6 61.4 0 6 10 1 0.1 1 1