This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGAATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.57 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 145,153 (5.9%) Reads that were too short: 3,456 (0.1%) Reads written (passing filters): 141,697 (5.8%) Total basepairs processed: 740,966,583 bp Total written (filtered): 39,739,276 bp (5.4%) === Adapter 1 === Sequence: CGAATC; Type: regular 5'; Length: 6; Trimmed: 145153 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3939 601.0 0 3939 7 57503 601.0 0 57503 8 1464 601.0 0 1464 9 51137 601.0 0 51137 10 55 601.0 0 55 11 29 601.0 0 29 12 23 601.0 0 23 13 25 601.0 0 25 14 24 601.0 0 24 15 15 601.0 0 15 16 20 601.0 0 20 17 31 601.0 0 31 18 26 601.0 0 26 19 31 601.0 0 31 20 44 601.0 0 44 21 26 601.0 0 26 22 19 601.0 0 19 23 38 601.0 0 38 24 44 601.0 0 44 25 28 601.0 0 28 26 25 601.0 0 25 27 70 601.0 0 70 28 35 601.0 0 35 29 35 601.0 0 35 30 55 601.0 0 55 31 31 601.0 0 31 32 27 601.0 0 27 33 72 601.0 0 72 34 80 601.0 0 80 35 50 601.0 0 50 36 29 601.0 0 29 37 90 601.0 0 90 38 26 601.0 0 26 39 32 601.0 0 32 40 38 601.0 0 38 41 36 601.0 0 36 42 53 601.0 0 53 43 57 601.0 0 57 44 44 601.0 0 44 45 31 601.0 0 31 46 42 601.0 0 42 47 61 601.0 0 61 48 56 601.0 0 56 49 89 601.0 0 89 50 140 601.0 0 140 51 108 601.0 0 108 52 194 601.0 0 194 53 222 601.0 0 222 54 2783 601.0 0 2783 55 4195 601.0 0 4195 56 10318 601.0 0 10318 57 60 601.0 0 60 58 36 601.0 0 36 59 22 601.0 0 22 60 33 601.0 0 33 61 20 601.0 0 20 62 23 601.0 0 23 63 21 601.0 0 21 64 15 601.0 0 15 65 12 601.0 0 12 66 22 601.0 0 22 67 23 601.0 0 23 68 42 601.0 0 42 69 41 601.0 0 41 70 25 601.0 0 25 71 12 601.0 0 12 72 28 601.0 0 28 73 37 601.0 0 37 74 14 601.0 0 14 75 21 601.0 0 21 76 20 601.0 0 20 77 37 601.0 0 37 78 18 601.0 0 18 79 20 601.0 0 20 80 34 601.0 0 34 81 31 601.0 0 31 82 28 601.0 0 28 83 30 601.0 0 30 84 49 601.0 0 49 85 23 601.0 0 23 86 30 601.0 0 30 87 30 601.0 0 30 88 46 601.0 0 46 89 25 601.0 0 25 90 27 601.0 0 27 91 27 601.0 0 27 92 51 601.0 0 51 93 57 601.0 0 57 94 1600 601.0 0 1600 95 592 601.0 0 592 96 270 601.0 0 270 97 22 601.0 0 22 98 17 601.0 0 17 99 39 601.0 0 39 100 16 601.0 0 16 101 11 601.0 0 11 102 21 601.0 0 21 103 21 601.0 0 21 104 29 601.0 0 29 105 18 601.0 0 18 106 17 601.0 0 17 107 28 601.0 0 28 108 41 601.0 0 41 109 70 601.0 0 70 110 176 601.0 0 176 111 208 601.0 0 208 112 16 601.0 0 16 113 9 601.0 0 9 114 22 601.0 0 22 115 26 601.0 0 26 116 20 601.0 0 20 117 23 601.0 0 23 118 25 601.0 0 25 119 38 601.0 0 38 120 93 601.0 0 93 121 460 601.0 0 460 122 158 601.0 0 158 123 98 601.0 0 98 124 24 601.0 0 24 125 27 601.0 0 27 126 30 601.0 0 30 127 25 601.0 0 25 128 53 601.0 0 53 129 29 601.0 0 29 130 19 601.0 0 19 131 25 601.0 0 25 132 12 601.0 0 12 133 29 601.0 0 29 134 19 601.0 0 19 135 39 601.0 0 39 136 31 601.0 0 31 137 32 601.0 0 32 138 28 601.0 0 28 139 42 601.0 0 42 140 70 601.0 0 70 141 49 601.0 0 49 142 28 601.0 0 28 143 25 601.0 0 25 144 30 601.0 0 30 145 34 601.0 0 34 146 31 601.0 0 31 147 32 601.0 0 32 148 41 601.0 0 41 149 32 601.0 0 32 150 15 601.0 0 15 151 18 601.0 0 18 152 19 601.0 0 19 153 38 601.0 0 38 154 37 601.0 0 37 155 20 601.0 0 20 156 29 601.0 0 29 157 48 601.0 0 48 158 24 601.0 0 24 159 36 601.0 0 36 160 133 601.0 0 133 161 77 601.0 0 77 162 44 601.0 0 44 163 24 601.0 0 24 164 21 601.0 0 21 165 41 601.0 0 41 166 49 601.0 0 49 167 51 601.0 0 51 168 23 601.0 0 23 169 24 601.0 0 24 170 19 601.0 0 19 171 21 601.0 0 21 172 12 601.0 0 12 173 34 601.0 0 34 174 30 601.0 0 30 175 49 601.0 0 49 176 45 601.0 0 45 177 89 601.0 0 89 178 126 601.0 0 126 179 25 601.0 0 25 180 36 601.0 0 36 181 60 601.0 0 60 182 82 601.0 0 82 183 24 601.0 0 24 184 15 601.0 0 15 185 31 601.0 0 31 186 21 601.0 0 21 187 20 601.0 0 20 188 45 601.0 0 45 189 22 601.0 0 22 190 25 601.0 0 25 191 24 601.0 0 24 192 21 601.0 0 21 193 33 601.0 0 33 194 40 601.0 0 40 195 26 601.0 0 26 196 34 601.0 0 34 197 32 601.0 0 32 198 37 601.0 0 37 199 39 601.0 0 39 200 31 601.0 0 31 201 23 601.0 0 23 202 24 601.0 0 24 203 39 601.0 0 39 204 35 601.0 0 35 205 33 601.0 0 33 206 24 601.0 0 24 207 27 601.0 0 27 208 28 601.0 0 28 209 38 601.0 0 38 210 16 601.0 0 16 211 21 601.0 0 21 212 22 601.0 0 22 213 31 601.0 0 31 214 20 601.0 0 20 215 23 601.0 0 23 216 16 601.0 0 16 217 23 601.0 0 23 218 16 601.0 0 16 219 26 601.0 0 26 220 19 601.0 0 19 221 22 601.0 0 22 222 41 601.0 0 41 223 27 601.0 0 27 224 37 601.0 0 37 225 24 601.0 0 24 226 29 601.0 0 29 227 37 601.0 0 37 228 39 601.0 0 39 229 52 601.0 0 52 230 42 601.0 0 42 231 21 601.0 0 21 232 24 601.0 0 24 233 22 601.0 0 22 234 34 601.0 0 34 235 17 601.0 0 17 236 29 601.0 0 29 237 22 601.0 0 22 238 20 601.0 0 20 239 38 601.0 0 38 240 29 601.0 0 29 241 18 601.0 0 18 242 10 601.0 0 10 243 18 601.0 0 18 244 19 601.0 0 19 245 25 601.0 0 25 246 21 601.0 0 21 247 42 601.0 0 42 248 29 601.0 0 29 249 39 601.0 0 39 250 17 601.0 0 17 251 16 601.0 0 16 252 29 601.0 0 29 253 34 601.0 0 34 254 37 601.0 0 37 255 26 601.0 0 26 256 60 601.0 0 60 257 32 601.0 0 32 258 33 601.0 0 33 259 28 601.0 0 28 260 33 601.0 0 33 261 27 601.0 0 27 262 60 601.0 0 60 263 79 601.0 0 79 264 79 601.0 0 79 265 16 601.0 0 16 266 42 601.0 0 42 267 25 601.0 0 25 268 26 601.0 0 26 269 33 601.0 0 33 270 23 601.0 0 23 271 29 601.0 0 29 272 37 601.0 0 37 273 32 601.0 0 32 274 48 601.0 0 48 275 46 601.0 0 46 276 31 601.0 0 31 277 27 601.0 0 27 278 96 601.0 0 96 279 61 601.0 0 61 280 44 601.0 0 44 281 13 601.0 0 13 282 44 601.0 0 44 283 63 601.0 0 63 284 78 601.0 0 78 285 45 601.0 0 45 286 28 601.0 0 28 287 30 601.0 0 30 288 25 601.0 0 25 289 23 601.0 0 23 290 19 601.0 0 19 291 61 601.0 0 61 292 67 601.0 0 67 293 28 601.0 0 28 294 42 601.0 0 42 295 34 601.0 0 34 296 40 601.0 0 40 297 31 601.0 0 31 298 24 601.0 0 24 299 52 601.0 0 52 300 114 601.0 0 114 301 81 601.0 0 81 Finished in 7.09 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 141,697 Reads with adapters: 61,390 (43.3%) Reads that were too short: 38 (0.0%) Reads written (passing filters): 61,352 (43.3%) Total basepairs processed: 39,739,276 bp Total written (filtered): 16,770,411 bp (42.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 61390 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 26 0.0 3 0 0 25 1 19 640 0.0 3 0 622 16 1 1 20 60082 0.0 4 59175 871 34 2 21 133 0.0 4 18 112 3 22 14 0.0 4 1 0 12 1 23 32 0.0 4 1 0 0 26 5 24 3 0.0 4 0 0 0 1 2 25 2 0.0 4 2 35 32 0.0 4 0 0 0 0 32 43 1 0.0 4 1 83 2 0.0 4 0 0 0 0 2 100 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 370 0.0 4 0 0 0 0 370 128 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 137 1 0.0 4 1 138 1 0.0 4 1 156 1 0.0 4 0 1 161 1 0.0 4 1 166 1 0.0 4 1 169 4 0.0 4 0 0 0 0 4 187 1 0.0 4 1 193 1 0.0 4 0 1 195 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 207 1 0.0 4 1 208 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 1 227 2 0.0 4 0 0 0 0 2 228 9 0.0 4 0 0 1 0 8 229 2 0.0 4 0 0 0 0 2 230 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 1 250 1 0.0 4 1 253 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 269 1 0.0 4 1 275 1 0.0 4 0 0 0 0 1 277 2 0.0 4 0 0 0 1 1 279 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 1 290 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 1 292 1 0.0 4 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.12 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 61,352 Reads with adapters: 1,170 (1.9%) Reads written (passing filters): 61,352 (100.0%) Total basepairs processed: 16,770,411 bp Total written (filtered): 16,748,090 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1170 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 24.2% C: 9.6% G: 63.8% T: 2.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 129 958.6 0 129 4 17 239.7 0 17 5 4 59.9 0 4 6 2 15.0 0 2 7 3 3.7 0 3 9 7 0.2 0 1 6 10 12 0.1 1 0 12 11 665 0.0 1 3 662 12 100 0.0 1 1 99 13 2 0.0 1 0 2 20 30 0.0 1 25 5 21 26 0.0 1 25 1 22 1 0.0 1 1 23 3 0.0 1 3 24 96 0.0 1 79 17 25 7 0.0 1 7 94 4 0.0 1 4 112 1 0.0 1 1 113 29 0.0 1 28 1 114 2 0.0 1 2 124 1 0.0 1 1 149 5 0.0 1 5 152 1 0.0 1 0 1 158 1 0.0 1 1 159 2 0.0 1 2 177 2 0.0 1 2 180 5 0.0 1 5 186 1 0.0 1 1 199 1 0.0 1 1 202 7 0.0 1 7 203 1 0.0 1 1 207 1 0.0 1 1 210 2 0.0 1 1 1