This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATGAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.24 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 81,354 (3.3%) Reads that were too short: 25,498 (1.0%) Reads written (passing filters): 55,856 (2.3%) Total basepairs processed: 735,190,694 bp Total written (filtered): 13,316,974 bp (1.8%) === Adapter 1 === Sequence: AATGAA; Type: regular 5'; Length: 6; Trimmed: 81354 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 246 596.3 0 246 7 371 596.3 0 371 8 14740 596.3 0 14740 9 11857 596.3 0 11857 10 58 596.3 0 58 11 36 596.3 0 36 12 36 596.3 0 36 13 35 596.3 0 35 14 25 596.3 0 25 15 23 596.3 0 23 16 21 596.3 0 21 17 22 596.3 0 22 18 22 596.3 0 22 19 28 596.3 0 28 20 19 596.3 0 19 21 28 596.3 0 28 22 28 596.3 0 28 23 17 596.3 0 17 24 15 596.3 0 15 25 21 596.3 0 21 26 26 596.3 0 26 27 33 596.3 0 33 28 32 596.3 0 32 29 29 596.3 0 29 30 29 596.3 0 29 31 22 596.3 0 22 32 65 596.3 0 65 33 25 596.3 0 25 34 9 596.3 0 9 35 46 596.3 0 46 36 18 596.3 0 18 37 22 596.3 0 22 38 23 596.3 0 23 39 32 596.3 0 32 40 27 596.3 0 27 41 38 596.3 0 38 42 33 596.3 0 33 43 71 596.3 0 71 44 36 596.3 0 36 45 84 596.3 0 84 46 45 596.3 0 45 47 62 596.3 0 62 48 23 596.3 0 23 49 29 596.3 0 29 50 17 596.3 0 17 51 28 596.3 0 28 52 16 596.3 0 16 53 25 596.3 0 25 54 18 596.3 0 18 55 32 596.3 0 32 56 29 596.3 0 29 57 41 596.3 0 41 58 40 596.3 0 40 59 36 596.3 0 36 60 33 596.3 0 33 61 29 596.3 0 29 62 59 596.3 0 59 63 36 596.3 0 36 64 37 596.3 0 37 65 41 596.3 0 41 66 84 596.3 0 84 67 36 596.3 0 36 68 51 596.3 0 51 69 84 596.3 0 84 70 601 596.3 0 601 71 64 596.3 0 64 72 93 596.3 0 93 73 115 596.3 0 115 74 294 596.3 0 294 75 164 596.3 0 164 76 138 596.3 0 138 77 201 596.3 0 201 78 219 596.3 0 219 79 4418 596.3 0 4418 80 1970 596.3 0 1970 81 2262 596.3 0 2262 82 145 596.3 0 145 83 271 596.3 0 271 84 222 596.3 0 222 85 39 596.3 0 39 86 51 596.3 0 51 87 43 596.3 0 43 88 49 596.3 0 49 89 34 596.3 0 34 90 38 596.3 0 38 91 26 596.3 0 26 92 52 596.3 0 52 93 26 596.3 0 26 94 30 596.3 0 30 95 50 596.3 0 50 96 32 596.3 0 32 97 58 596.3 0 58 98 49 596.3 0 49 99 65 596.3 0 65 100 111 596.3 0 111 101 113 596.3 0 113 102 60 596.3 0 60 103 54 596.3 0 54 104 66 596.3 0 66 105 53 596.3 0 53 106 52 596.3 0 52 107 62 596.3 0 62 108 73 596.3 0 73 109 88 596.3 0 88 110 56 596.3 0 56 111 69 596.3 0 69 112 97 596.3 0 97 113 54 596.3 0 54 114 62 596.3 0 62 115 94 596.3 0 94 116 77 596.3 0 77 117 93 596.3 0 93 118 97 596.3 0 97 119 95 596.3 0 95 120 107 596.3 0 107 121 103 596.3 0 103 122 73 596.3 0 73 123 103 596.3 0 103 124 170 596.3 0 170 125 472 596.3 0 472 126 603 596.3 0 603 127 476 596.3 0 476 128 141 596.3 0 141 129 189 596.3 0 189 130 247 596.3 0 247 131 88 596.3 0 88 132 71 596.3 0 71 133 72 596.3 0 72 134 74 596.3 0 74 135 86 596.3 0 86 136 108 596.3 0 108 137 158 596.3 0 158 138 186 596.3 0 186 139 1035 596.3 0 1035 140 447 596.3 0 447 141 171 596.3 0 171 142 135 596.3 0 135 143 133 596.3 0 133 144 100 596.3 0 100 145 56 596.3 0 56 146 72 596.3 0 72 147 65 596.3 0 65 148 52 596.3 0 52 149 60 596.3 0 60 150 78 596.3 0 78 151 105 596.3 0 105 152 104 596.3 0 104 153 57 596.3 0 57 154 57 596.3 0 57 155 57 596.3 0 57 156 59 596.3 0 59 157 89 596.3 0 89 158 205 596.3 0 205 159 379 596.3 0 379 160 635 596.3 0 635 161 50 596.3 0 50 162 65 596.3 0 65 163 103 596.3 0 103 164 151 596.3 0 151 165 263 596.3 0 263 166 72 596.3 0 72 167 82 596.3 0 82 168 62 596.3 0 62 169 60 596.3 0 60 170 54 596.3 0 54 171 54 596.3 0 54 172 63 596.3 0 63 173 40 596.3 0 40 174 53 596.3 0 53 175 54 596.3 0 54 176 65 596.3 0 65 177 89 596.3 0 89 178 91 596.3 0 91 179 61 596.3 0 61 180 79 596.3 0 79 181 125 596.3 0 125 182 1039 596.3 0 1039 183 699 596.3 0 699 184 1183 596.3 0 1183 185 66 596.3 0 66 186 44 596.3 0 44 187 54 596.3 0 54 188 38 596.3 0 38 189 56 596.3 0 56 190 47 596.3 0 47 191 52 596.3 0 52 192 40 596.3 0 40 193 42 596.3 0 42 194 57 596.3 0 57 195 52 596.3 0 52 196 59 596.3 0 59 197 61 596.3 0 61 198 56 596.3 0 56 199 57 596.3 0 57 200 52 596.3 0 52 201 64 596.3 0 64 202 61 596.3 0 61 203 46 596.3 0 46 204 64 596.3 0 64 205 81 596.3 0 81 206 119 596.3 0 119 207 110 596.3 0 110 208 105 596.3 0 105 209 85 596.3 0 85 210 101 596.3 0 101 211 100 596.3 0 100 212 110 596.3 0 110 213 107 596.3 0 107 214 77 596.3 0 77 215 49 596.3 0 49 216 68 596.3 0 68 217 61 596.3 0 61 218 77 596.3 0 77 219 68 596.3 0 68 220 54 596.3 0 54 221 98 596.3 0 98 222 58 596.3 0 58 223 70 596.3 0 70 224 76 596.3 0 76 225 112 596.3 0 112 226 222 596.3 0 222 227 179 596.3 0 179 228 153 596.3 0 153 229 42 596.3 0 42 230 46 596.3 0 46 231 71 596.3 0 71 232 76 596.3 0 76 233 114 596.3 0 114 234 66 596.3 0 66 235 68 596.3 0 68 236 114 596.3 0 114 237 105 596.3 0 105 238 125 596.3 0 125 239 124 596.3 0 124 240 70 596.3 0 70 241 76 596.3 0 76 242 64 596.3 0 64 243 63 596.3 0 63 244 76 596.3 0 76 245 58 596.3 0 58 246 102 596.3 0 102 247 83 596.3 0 83 248 133 596.3 0 133 249 147 596.3 0 147 250 151 596.3 0 151 251 198 596.3 0 198 252 252 596.3 0 252 253 2363 596.3 0 2363 254 2363 596.3 0 2363 255 11013 596.3 0 11013 256 450 596.3 0 450 257 169 596.3 0 169 258 71 596.3 0 71 259 86 596.3 0 86 260 193 596.3 0 193 261 77 596.3 0 77 262 65 596.3 0 65 263 130 596.3 0 130 264 228 596.3 0 228 265 211 596.3 0 211 266 350 596.3 0 350 267 42 596.3 0 42 268 52 596.3 0 52 269 76 596.3 0 76 270 65 596.3 0 65 271 58 596.3 0 58 272 55 596.3 0 55 273 49 596.3 0 49 274 82 596.3 0 82 275 88 596.3 0 88 276 104 596.3 0 104 277 108 596.3 0 108 278 75 596.3 0 75 279 49 596.3 0 49 280 58 596.3 0 58 281 71 596.3 0 71 282 68 596.3 0 68 283 63 596.3 0 63 284 65 596.3 0 65 285 84 596.3 0 84 286 66 596.3 0 66 287 53 596.3 0 53 288 88 596.3 0 88 289 120 596.3 0 120 290 138 596.3 0 138 291 132 596.3 0 132 292 74 596.3 0 74 293 73 596.3 0 73 294 74 596.3 0 74 295 73 596.3 0 73 296 83 596.3 0 83 297 94 596.3 0 94 298 102 596.3 0 102 299 87 596.3 0 87 300 83 596.3 0 83 301 142 596.3 0 142 Finished in 2.48 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 55,856 Reads with adapters: 14,977 (26.8%) Reads that were too short: 202 (0.4%) Reads written (passing filters): 14,775 (26.5%) Total basepairs processed: 13,316,974 bp Total written (filtered): 4,056,459 bp (30.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 14977 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 15 0.0 3 0 0 10 5 16 176 0.0 3 0 158 9 9 17 14312 0.0 3 13418 753 116 25 18 53 0.0 3 6 39 3 5 19 36 0.0 3 0 0 26 10 20 41 0.0 3 0 0 0 41 21 10 0.0 3 1 1 1 7 22 1 0.0 3 0 0 0 1 25 14 0.0 3 0 0 0 14 28 8 0.0 3 0 0 0 8 30 1 0.0 3 0 0 0 1 31 2 0.0 3 0 0 0 2 32 1 0.0 3 0 0 0 1 39 2 0.0 3 0 0 0 2 40 1 0.0 3 0 0 0 1 41 12 0.0 3 0 0 0 12 44 5 0.0 3 0 0 0 5 50 2 0.0 3 1 0 0 1 52 9 0.0 3 0 0 0 9 53 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 65 5 0.0 3 0 0 0 5 67 5 0.0 3 0 0 0 5 68 2 0.0 3 0 0 0 2 71 3 0.0 3 0 0 0 3 75 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 2 0.0 3 0 0 0 2 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 92 3 0.0 3 0 0 0 3 93 4 0.0 3 0 0 0 4 94 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 103 1 0.0 3 0 0 0 1 105 3 0.0 3 0 0 0 3 107 2 0.0 3 0 0 0 2 108 2 0.0 3 0 0 0 2 109 3 0.0 3 0 0 0 3 112 22 0.0 3 0 0 0 22 113 3 0.0 3 0 0 0 3 114 1 0.0 3 0 0 0 1 115 3 0.0 3 0 0 0 3 116 2 0.0 3 0 0 0 2 117 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 4 0.0 3 0 0 0 4 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 134 4 0.0 3 0 0 0 4 135 2 0.0 3 0 0 0 2 136 9 0.0 3 0 0 0 9 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 3 0.0 3 0 0 0 3 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 2 0.0 3 0 0 0 2 149 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 153 3 0.0 3 0 0 0 3 154 3 0.0 3 0 0 0 3 155 2 0.0 3 0 0 0 2 160 1 0.0 3 0 0 0 1 163 3 0.0 3 0 0 0 3 165 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 1 1 172 12 0.0 3 0 0 0 12 177 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 185 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 187 2 0.0 3 0 0 0 2 193 1 0.0 3 0 0 0 1 194 3 0.0 3 0 0 0 3 196 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 205 3 0.0 3 0 0 0 3 211 1 0.0 3 0 0 0 1 213 12 0.0 3 0 0 0 12 214 35 0.0 3 0 0 2 33 215 2 0.0 3 0 0 1 1 217 43 0.0 3 0 0 3 40 218 2 0.0 3 0 0 0 2 220 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 281 5 0.0 3 0 0 1 4 282 1 0.0 3 0 0 0 1 288 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.50 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 14,775 Reads with adapters: 51 (0.3%) Reads written (passing filters): 14,775 (100.0%) Total basepairs processed: 4,056,459 bp Total written (filtered): 4,056,303 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 51 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 27.5% C: 29.4% G: 37.3% T: 5.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 48 230.9 0 48 4 3 57.7 0 3