This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATGAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 62.92 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 91,465 (3.7%) Reads that were too short: 32,638 (1.3%) Reads written (passing filters): 58,827 (2.4%) Total basepairs processed: 735,190,694 bp Total written (filtered): 13,996,564 bp (1.9%) === Adapter 1 === Sequence: AATGAA; Type: regular 5'; Length: 6; Trimmed: 91465 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 173 596.3 0 173 7 291 596.3 0 291 8 13117 596.3 0 13117 9 14984 596.3 0 14984 10 57 596.3 0 57 11 32 596.3 0 32 12 38 596.3 0 38 13 33 596.3 0 33 14 42 596.3 0 42 15 36 596.3 0 36 16 40 596.3 0 40 17 32 596.3 0 32 18 31 596.3 0 31 19 31 596.3 0 31 20 30 596.3 0 30 21 33 596.3 0 33 22 41 596.3 0 41 23 33 596.3 0 33 24 27 596.3 0 27 25 47 596.3 0 47 26 41 596.3 0 41 27 49 596.3 0 49 28 40 596.3 0 40 29 38 596.3 0 38 30 38 596.3 0 38 31 30 596.3 0 30 32 47 596.3 0 47 33 30 596.3 0 30 34 34 596.3 0 34 35 44 596.3 0 44 36 63 596.3 0 63 37 45 596.3 0 45 38 58 596.3 0 58 39 39 596.3 0 39 40 33 596.3 0 33 41 60 596.3 0 60 42 45 596.3 0 45 43 80 596.3 0 80 44 45 596.3 0 45 45 90 596.3 0 90 46 51 596.3 0 51 47 70 596.3 0 70 48 35 596.3 0 35 49 44 596.3 0 44 50 45 596.3 0 45 51 52 596.3 0 52 52 47 596.3 0 47 53 39 596.3 0 39 54 33 596.3 0 33 55 42 596.3 0 42 56 50 596.3 0 50 57 44 596.3 0 44 58 33 596.3 0 33 59 46 596.3 0 46 60 38 596.3 0 38 61 36 596.3 0 36 62 57 596.3 0 57 63 35 596.3 0 35 64 43 596.3 0 43 65 63 596.3 0 63 66 111 596.3 0 111 67 40 596.3 0 40 68 70 596.3 0 70 69 102 596.3 0 102 70 699 596.3 0 699 71 72 596.3 0 72 72 76 596.3 0 76 73 106 596.3 0 106 74 78 596.3 0 78 75 81 596.3 0 81 76 85 596.3 0 85 77 165 596.3 0 165 78 190 596.3 0 190 79 4329 596.3 0 4329 80 1906 596.3 0 1906 81 2397 596.3 0 2397 82 145 596.3 0 145 83 176 596.3 0 176 84 200 596.3 0 200 85 69 596.3 0 69 86 79 596.3 0 79 87 71 596.3 0 71 88 109 596.3 0 109 89 70 596.3 0 70 90 63 596.3 0 63 91 79 596.3 0 79 92 95 596.3 0 95 93 50 596.3 0 50 94 57 596.3 0 57 95 81 596.3 0 81 96 73 596.3 0 73 97 90 596.3 0 90 98 87 596.3 0 87 99 118 596.3 0 118 100 85 596.3 0 85 101 74 596.3 0 74 102 77 596.3 0 77 103 88 596.3 0 88 104 84 596.3 0 84 105 82 596.3 0 82 106 64 596.3 0 64 107 78 596.3 0 78 108 96 596.3 0 96 109 89 596.3 0 89 110 79 596.3 0 79 111 71 596.3 0 71 112 101 596.3 0 101 113 77 596.3 0 77 114 71 596.3 0 71 115 99 596.3 0 99 116 114 596.3 0 114 117 129 596.3 0 129 118 85 596.3 0 85 119 106 596.3 0 106 120 97 596.3 0 97 121 119 596.3 0 119 122 91 596.3 0 91 123 101 596.3 0 101 124 155 596.3 0 155 125 297 596.3 0 297 126 382 596.3 0 382 127 331 596.3 0 331 128 135 596.3 0 135 129 167 596.3 0 167 130 251 596.3 0 251 131 86 596.3 0 86 132 82 596.3 0 82 133 82 596.3 0 82 134 85 596.3 0 85 135 92 596.3 0 92 136 164 596.3 0 164 137 165 596.3 0 165 138 198 596.3 0 198 139 975 596.3 0 975 140 462 596.3 0 462 141 142 596.3 0 142 142 125 596.3 0 125 143 143 596.3 0 143 144 111 596.3 0 111 145 96 596.3 0 96 146 102 596.3 0 102 147 76 596.3 0 76 148 83 596.3 0 83 149 67 596.3 0 67 150 110 596.3 0 110 151 126 596.3 0 126 152 106 596.3 0 106 153 84 596.3 0 84 154 75 596.3 0 75 155 83 596.3 0 83 156 95 596.3 0 95 157 70 596.3 0 70 158 192 596.3 0 192 159 437 596.3 0 437 160 632 596.3 0 632 161 70 596.3 0 70 162 72 596.3 0 72 163 96 596.3 0 96 164 206 596.3 0 206 165 335 596.3 0 335 166 74 596.3 0 74 167 99 596.3 0 99 168 83 596.3 0 83 169 82 596.3 0 82 170 97 596.3 0 97 171 111 596.3 0 111 172 69 596.3 0 69 173 75 596.3 0 75 174 63 596.3 0 63 175 75 596.3 0 75 176 80 596.3 0 80 177 89 596.3 0 89 178 101 596.3 0 101 179 98 596.3 0 98 180 95 596.3 0 95 181 123 596.3 0 123 182 896 596.3 0 896 183 742 596.3 0 742 184 1239 596.3 0 1239 185 72 596.3 0 72 186 56 596.3 0 56 187 74 596.3 0 74 188 90 596.3 0 90 189 79 596.3 0 79 190 72 596.3 0 72 191 65 596.3 0 65 192 86 596.3 0 86 193 64 596.3 0 64 194 73 596.3 0 73 195 94 596.3 0 94 196 75 596.3 0 75 197 103 596.3 0 103 198 68 596.3 0 68 199 82 596.3 0 82 200 81 596.3 0 81 201 83 596.3 0 83 202 86 596.3 0 86 203 101 596.3 0 101 204 87 596.3 0 87 205 89 596.3 0 89 206 112 596.3 0 112 207 114 596.3 0 114 208 108 596.3 0 108 209 106 596.3 0 106 210 101 596.3 0 101 211 115 596.3 0 115 212 107 596.3 0 107 213 90 596.3 0 90 214 87 596.3 0 87 215 88 596.3 0 88 216 105 596.3 0 105 217 78 596.3 0 78 218 103 596.3 0 103 219 89 596.3 0 89 220 67 596.3 0 67 221 115 596.3 0 115 222 89 596.3 0 89 223 98 596.3 0 98 224 96 596.3 0 96 225 133 596.3 0 133 226 253 596.3 0 253 227 186 596.3 0 186 228 158 596.3 0 158 229 70 596.3 0 70 230 76 596.3 0 76 231 92 596.3 0 92 232 109 596.3 0 109 233 107 596.3 0 107 234 95 596.3 0 95 235 104 596.3 0 104 236 171 596.3 0 171 237 103 596.3 0 103 238 126 596.3 0 126 239 94 596.3 0 94 240 98 596.3 0 98 241 98 596.3 0 98 242 104 596.3 0 104 243 107 596.3 0 107 244 74 596.3 0 74 245 82 596.3 0 82 246 132 596.3 0 132 247 99 596.3 0 99 248 160 596.3 0 160 249 201 596.3 0 201 250 195 596.3 0 195 251 247 596.3 0 247 252 306 596.3 0 306 253 3048 596.3 0 3048 254 3198 596.3 0 3198 255 15944 596.3 0 15944 256 562 596.3 0 562 257 320 596.3 0 320 258 65 596.3 0 65 259 74 596.3 0 74 260 125 596.3 0 125 261 83 596.3 0 83 262 74 596.3 0 74 263 77 596.3 0 77 264 125 596.3 0 125 265 118 596.3 0 118 266 151 596.3 0 151 267 49 596.3 0 49 268 65 596.3 0 65 269 71 596.3 0 71 270 73 596.3 0 73 271 76 596.3 0 76 272 70 596.3 0 70 273 80 596.3 0 80 274 73 596.3 0 73 275 84 596.3 0 84 276 115 596.3 0 115 277 90 596.3 0 90 278 78 596.3 0 78 279 59 596.3 0 59 280 68 596.3 0 68 281 66 596.3 0 66 282 70 596.3 0 70 283 65 596.3 0 65 284 79 596.3 0 79 285 64 596.3 0 64 286 94 596.3 0 94 287 69 596.3 0 69 288 64 596.3 0 64 289 105 596.3 0 105 290 98 596.3 0 98 291 95 596.3 0 95 292 63 596.3 0 63 293 77 596.3 0 77 294 66 596.3 0 66 295 69 596.3 0 69 296 65 596.3 0 65 297 67 596.3 0 67 298 86 596.3 0 86 299 73 596.3 0 73 300 75 596.3 0 75 301 102 596.3 0 102 Finished in 2.89 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 58,827 Reads with adapters: 15,673 (26.6%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 15,661 (26.6%) Total basepairs processed: 13,996,564 bp Total written (filtered): 4,240,170 bp (30.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 15673 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 10 0.0 3 0 0 9 0 1 19 211 0.0 3 0 206 4 0 1 20 15196 0.0 4 14884 297 12 1 2 21 30 0.0 4 5 22 2 1 22 5 0.0 4 0 0 4 0 1 23 18 0.0 4 3 0 0 15 41 1 0.0 4 0 0 0 0 1 43 2 0.0 4 0 0 0 0 2 59 1 0.0 4 0 0 0 0 1 67 10 0.0 4 0 0 0 0 10 77 1 0.0 4 0 0 0 0 1 78 2 0.0 4 0 0 0 0 2 86 1 0.0 4 0 0 0 0 1 95 1 0.0 4 1 109 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 126 4 0.0 4 0 0 0 0 4 127 170 0.0 4 0 0 0 0 170 128 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.55 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 15,661 Reads with adapters: 1,868 (11.9%) Reads written (passing filters): 15,661 (100.0%) Total basepairs processed: 4,240,170 bp Total written (filtered): 4,220,666 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1868 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.5% C: 2.7% G: 93.9% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 244.7 0 2 4 1 61.2 0 1 8 1 0.2 0 1 9 629 0.1 0 20 609 10 1065 0.0 1 30 1035 11 162 0.0 1 1 161 12 1 0.0 1 0 1 13 1 0.0 1 0 1 208 1 0.0 1 0 1 223 4 0.0 1 3 1 268 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.