This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.42 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 125,753 (5.1%) Reads that were too short: 11,580 (0.5%) Reads written (passing filters): 114,173 (4.7%) Total basepairs processed: 735,190,694 bp Total written (filtered): 32,388,436 bp (4.4%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 125753 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1684 596.3 0 1684 7 56333 596.3 0 56333 8 46319 596.3 0 46319 9 73 596.3 0 73 10 18 596.3 0 18 11 21 596.3 0 21 12 16 596.3 0 16 13 33 596.3 0 33 14 27 596.3 0 27 15 20 596.3 0 20 16 43 596.3 0 43 17 23 596.3 0 23 18 25 596.3 0 25 19 22 596.3 0 22 20 20 596.3 0 20 21 20 596.3 0 20 22 24 596.3 0 24 23 19 596.3 0 19 24 21 596.3 0 21 25 20 596.3 0 20 26 108 596.3 0 108 27 59 596.3 0 59 28 112 596.3 0 112 29 16 596.3 0 16 30 12 596.3 0 12 31 22 596.3 0 22 32 25 596.3 0 25 33 18 596.3 0 18 34 13 596.3 0 13 35 25 596.3 0 25 36 17 596.3 0 17 37 28 596.3 0 28 38 20 596.3 0 20 39 23 596.3 0 23 40 20 596.3 0 20 41 21 596.3 0 21 42 23 596.3 0 23 43 17 596.3 0 17 44 13 596.3 0 13 45 25 596.3 0 25 46 11 596.3 0 11 47 21 596.3 0 21 48 15 596.3 0 15 49 22 596.3 0 22 50 12 596.3 0 12 51 27 596.3 0 27 52 20 596.3 0 20 53 19 596.3 0 19 54 21 596.3 0 21 55 19 596.3 0 19 56 13 596.3 0 13 57 15 596.3 0 15 58 15 596.3 0 15 59 21 596.3 0 21 60 23 596.3 0 23 61 19 596.3 0 19 62 18 596.3 0 18 63 23 596.3 0 23 64 16 596.3 0 16 65 24 596.3 0 24 66 13 596.3 0 13 67 19 596.3 0 19 68 22 596.3 0 22 69 27 596.3 0 27 70 13 596.3 0 13 71 18 596.3 0 18 72 13 596.3 0 13 73 17 596.3 0 17 74 17 596.3 0 17 75 49 596.3 0 49 76 27 596.3 0 27 77 48 596.3 0 48 78 33 596.3 0 33 79 35 596.3 0 35 80 37 596.3 0 37 81 35 596.3 0 35 82 30 596.3 0 30 83 27 596.3 0 27 84 23 596.3 0 23 85 29 596.3 0 29 86 79 596.3 0 79 87 19 596.3 0 19 88 18 596.3 0 18 89 20 596.3 0 20 90 20 596.3 0 20 91 29 596.3 0 29 92 31 596.3 0 31 93 16 596.3 0 16 94 32 596.3 0 32 95 32 596.3 0 32 96 42 596.3 0 42 97 42 596.3 0 42 98 35 596.3 0 35 99 50 596.3 0 50 100 89 596.3 0 89 101 73 596.3 0 73 102 51 596.3 0 51 103 52 596.3 0 52 104 36 596.3 0 36 105 73 596.3 0 73 106 107 596.3 0 107 107 71 596.3 0 71 108 297 596.3 0 297 109 226 596.3 0 226 110 445 596.3 0 445 111 85 596.3 0 85 112 65 596.3 0 65 113 83 596.3 0 83 114 127 596.3 0 127 115 74 596.3 0 74 116 146 596.3 0 146 117 46 596.3 0 46 118 37 596.3 0 37 119 39 596.3 0 39 120 30 596.3 0 30 121 41 596.3 0 41 122 61 596.3 0 61 123 66 596.3 0 66 124 48 596.3 0 48 125 39 596.3 0 39 126 47 596.3 0 47 127 33 596.3 0 33 128 42 596.3 0 42 129 57 596.3 0 57 130 69 596.3 0 69 131 93 596.3 0 93 132 59 596.3 0 59 133 56 596.3 0 56 134 59 596.3 0 59 135 73 596.3 0 73 136 101 596.3 0 101 137 88 596.3 0 88 138 67 596.3 0 67 139 80 596.3 0 80 140 73 596.3 0 73 141 78 596.3 0 78 142 52 596.3 0 52 143 67 596.3 0 67 144 60 596.3 0 60 145 85 596.3 0 85 146 56 596.3 0 56 147 38 596.3 0 38 148 65 596.3 0 65 149 44 596.3 0 44 150 47 596.3 0 47 151 43 596.3 0 43 152 30 596.3 0 30 153 58 596.3 0 58 154 57 596.3 0 57 155 112 596.3 0 112 156 188 596.3 0 188 157 110 596.3 0 110 158 77 596.3 0 77 159 165 596.3 0 165 160 71 596.3 0 71 161 59 596.3 0 59 162 66 596.3 0 66 163 156 596.3 0 156 164 78 596.3 0 78 165 85 596.3 0 85 166 67 596.3 0 67 167 67 596.3 0 67 168 52 596.3 0 52 169 67 596.3 0 67 170 52 596.3 0 52 171 44 596.3 0 44 172 35 596.3 0 35 173 40 596.3 0 40 174 37 596.3 0 37 175 39 596.3 0 39 176 59 596.3 0 59 177 46 596.3 0 46 178 72 596.3 0 72 179 64 596.3 0 64 180 56 596.3 0 56 181 53 596.3 0 53 182 58 596.3 0 58 183 60 596.3 0 60 184 67 596.3 0 67 185 67 596.3 0 67 186 45 596.3 0 45 187 50 596.3 0 50 188 61 596.3 0 61 189 59 596.3 0 59 190 52 596.3 0 52 191 47 596.3 0 47 192 79 596.3 0 79 193 44 596.3 0 44 194 48 596.3 0 48 195 50 596.3 0 50 196 60 596.3 0 60 197 48 596.3 0 48 198 43 596.3 0 43 199 51 596.3 0 51 200 51 596.3 0 51 201 63 596.3 0 63 202 134 596.3 0 134 203 91 596.3 0 91 204 77 596.3 0 77 205 67 596.3 0 67 206 40 596.3 0 40 207 59 596.3 0 59 208 112 596.3 0 112 209 142 596.3 0 142 210 131 596.3 0 131 211 93 596.3 0 93 212 40 596.3 0 40 213 56 596.3 0 56 214 38 596.3 0 38 215 51 596.3 0 51 216 64 596.3 0 64 217 47 596.3 0 47 218 84 596.3 0 84 219 39 596.3 0 39 220 54 596.3 0 54 221 58 596.3 0 58 222 87 596.3 0 87 223 134 596.3 0 134 224 104 596.3 0 104 225 100 596.3 0 100 226 96 596.3 0 96 227 72 596.3 0 72 228 45 596.3 0 45 229 50 596.3 0 50 230 52 596.3 0 52 231 37 596.3 0 37 232 59 596.3 0 59 233 46 596.3 0 46 234 50 596.3 0 50 235 60 596.3 0 60 236 70 596.3 0 70 237 48 596.3 0 48 238 56 596.3 0 56 239 110 596.3 0 110 240 68 596.3 0 68 241 65 596.3 0 65 242 263 596.3 0 263 243 113 596.3 0 113 244 194 596.3 0 194 245 140 596.3 0 140 246 243 596.3 0 243 247 56 596.3 0 56 248 54 596.3 0 54 249 54 596.3 0 54 250 64 596.3 0 64 251 45 596.3 0 45 252 92 596.3 0 92 253 294 596.3 0 294 254 206 596.3 0 206 255 716 596.3 0 716 256 94 596.3 0 94 257 202 596.3 0 202 258 744 596.3 0 744 259 559 596.3 0 559 260 584 596.3 0 584 261 62 596.3 0 62 262 66 596.3 0 66 263 123 596.3 0 123 264 152 596.3 0 152 265 207 596.3 0 207 266 39 596.3 0 39 267 44 596.3 0 44 268 37 596.3 0 37 269 83 596.3 0 83 270 99 596.3 0 99 271 210 596.3 0 210 272 113 596.3 0 113 273 236 596.3 0 236 274 80 596.3 0 80 275 51 596.3 0 51 276 79 596.3 0 79 277 77 596.3 0 77 278 68 596.3 0 68 279 48 596.3 0 48 280 63 596.3 0 63 281 78 596.3 0 78 282 64 596.3 0 64 283 103 596.3 0 103 284 126 596.3 0 126 285 143 596.3 0 143 286 91 596.3 0 91 287 54 596.3 0 54 288 46 596.3 0 46 289 53 596.3 0 53 290 143 596.3 0 143 291 254 596.3 0 254 292 196 596.3 0 196 293 67 596.3 0 67 294 59 596.3 0 59 295 62 596.3 0 62 296 58 596.3 0 58 297 74 596.3 0 74 298 86 596.3 0 86 299 93 596.3 0 93 300 70 596.3 0 70 301 120 596.3 0 120 Finished in 5.41 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 114,173 Reads with adapters: 56,871 (49.8%) Reads that were too short: 111 (0.1%) Reads written (passing filters): 56,760 (49.7%) Total basepairs processed: 32,388,436 bp Total written (filtered): 15,718,104 bp (48.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 56871 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 8 0.0 2 0 0 0 8 15 43 0.0 3 0 0 28 15 16 634 0.0 3 0 524 58 52 17 55583 0.0 3 52282 2727 461 113 18 126 0.0 3 12 90 18 6 19 225 0.0 3 0 0 145 80 20 104 0.0 3 2 2 0 100 23 1 0.0 3 0 0 0 1 28 2 0.0 3 0 0 0 2 29 1 0.0 3 0 0 0 1 31 3 0.0 3 2 0 0 1 32 1 0.0 3 0 0 0 1 33 2 0.0 3 1 0 0 1 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 0 2 60 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 2 0.0 3 0 0 0 2 82 3 0.0 3 0 0 0 3 84 2 0.0 3 0 0 0 2 85 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 1 1 99 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 178 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 1 185 2 0.0 3 1 0 1 192 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 203 2 0.0 3 0 0 0 2 205 5 0.0 3 0 0 0 5 206 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 39 0.0 3 0 0 3 36 218 2 0.0 3 0 0 0 2 221 3 0.0 3 0 0 0 3 225 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 234 2 0.0 3 0 0 0 2 236 1 0.0 3 0 0 0 1 251 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 278 2 0.0 3 0 0 0 2 279 10 0.0 3 0 0 0 10 280 4 0.0 3 0 0 0 4 281 6 0.0 3 0 0 0 6 282 3 0.0 3 0 0 0 3 283 2 0.0 3 0 0 0 2 285 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 288 2 0.0 3 0 0 0 2 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.01 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 56,760 Reads with adapters: 1,208 (2.1%) Reads written (passing filters): 56,760 (100.0%) Total basepairs processed: 15,718,104 bp Total written (filtered): 15,714,451 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1208 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 21.4% C: 59.4% G: 14.2% T: 5.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1192 886.9 0 1192 4 6 221.7 0 6 5 7 55.4 0 7 6 3 13.9 0 3