This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.10 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 125,679 (5.1%) Reads that were too short: 9,924 (0.4%) Reads written (passing filters): 115,755 (4.7%) Total basepairs processed: 735,190,694 bp Total written (filtered): 33,086,136 bp (4.5%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 125679 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 880 596.3 0 880 7 50645 596.3 0 50645 8 56957 596.3 0 56957 9 33 596.3 0 33 10 16 596.3 0 16 11 17 596.3 0 17 12 10 596.3 0 10 13 15 596.3 0 15 14 12 596.3 0 12 15 13 596.3 0 13 16 21 596.3 0 21 17 16 596.3 0 16 18 17 596.3 0 17 19 7 596.3 0 7 20 9 596.3 0 9 21 8 596.3 0 8 22 9 596.3 0 9 23 16 596.3 0 16 24 15 596.3 0 15 25 5 596.3 0 5 26 13 596.3 0 13 27 10 596.3 0 10 28 26 596.3 0 26 29 13 596.3 0 13 30 10 596.3 0 10 31 7 596.3 0 7 32 5 596.3 0 5 33 17 596.3 0 17 34 10 596.3 0 10 35 15 596.3 0 15 36 12 596.3 0 12 37 16 596.3 0 16 38 11 596.3 0 11 39 14 596.3 0 14 40 11 596.3 0 11 41 10 596.3 0 10 42 10 596.3 0 10 43 4 596.3 0 4 44 9 596.3 0 9 45 9 596.3 0 9 46 9 596.3 0 9 47 9 596.3 0 9 48 6 596.3 0 6 49 14 596.3 0 14 50 10 596.3 0 10 51 9 596.3 0 9 52 9 596.3 0 9 53 6 596.3 0 6 54 8 596.3 0 8 55 8 596.3 0 8 56 10 596.3 0 10 57 10 596.3 0 10 58 9 596.3 0 9 59 16 596.3 0 16 60 23 596.3 0 23 61 7 596.3 0 7 62 15 596.3 0 15 63 21 596.3 0 21 64 6 596.3 0 6 65 16 596.3 0 16 66 11 596.3 0 11 67 17 596.3 0 17 68 9 596.3 0 9 69 8 596.3 0 8 70 11 596.3 0 11 71 15 596.3 0 15 72 13 596.3 0 13 73 8 596.3 0 8 74 13 596.3 0 13 75 14 596.3 0 14 76 14 596.3 0 14 77 20 596.3 0 20 78 9 596.3 0 9 79 13 596.3 0 13 80 12 596.3 0 12 81 27 596.3 0 27 82 21 596.3 0 21 83 25 596.3 0 25 84 16 596.3 0 16 85 24 596.3 0 24 86 90 596.3 0 90 87 26 596.3 0 26 88 23 596.3 0 23 89 28 596.3 0 28 90 27 596.3 0 27 91 19 596.3 0 19 92 41 596.3 0 41 93 24 596.3 0 24 94 25 596.3 0 25 95 24 596.3 0 24 96 55 596.3 0 55 97 38 596.3 0 38 98 30 596.3 0 30 99 38 596.3 0 38 100 55 596.3 0 55 101 33 596.3 0 33 102 23 596.3 0 23 103 24 596.3 0 24 104 24 596.3 0 24 105 42 596.3 0 42 106 110 596.3 0 110 107 69 596.3 0 69 108 173 596.3 0 173 109 147 596.3 0 147 110 297 596.3 0 297 111 53 596.3 0 53 112 66 596.3 0 66 113 58 596.3 0 58 114 129 596.3 0 129 115 73 596.3 0 73 116 112 596.3 0 112 117 45 596.3 0 45 118 30 596.3 0 30 119 27 596.3 0 27 120 17 596.3 0 17 121 27 596.3 0 27 122 44 596.3 0 44 123 48 596.3 0 48 124 44 596.3 0 44 125 26 596.3 0 26 126 37 596.3 0 37 127 27 596.3 0 27 128 50 596.3 0 50 129 71 596.3 0 71 130 53 596.3 0 53 131 85 596.3 0 85 132 38 596.3 0 38 133 40 596.3 0 40 134 47 596.3 0 47 135 48 596.3 0 48 136 77 596.3 0 77 137 75 596.3 0 75 138 40 596.3 0 40 139 47 596.3 0 47 140 47 596.3 0 47 141 58 596.3 0 58 142 50 596.3 0 50 143 45 596.3 0 45 144 44 596.3 0 44 145 54 596.3 0 54 146 33 596.3 0 33 147 39 596.3 0 39 148 42 596.3 0 42 149 27 596.3 0 27 150 48 596.3 0 48 151 38 596.3 0 38 152 48 596.3 0 48 153 58 596.3 0 58 154 41 596.3 0 41 155 111 596.3 0 111 156 131 596.3 0 131 157 141 596.3 0 141 158 57 596.3 0 57 159 137 596.3 0 137 160 46 596.3 0 46 161 53 596.3 0 53 162 45 596.3 0 45 163 156 596.3 0 156 164 56 596.3 0 56 165 76 596.3 0 76 166 42 596.3 0 42 167 54 596.3 0 54 168 44 596.3 0 44 169 52 596.3 0 52 170 33 596.3 0 33 171 40 596.3 0 40 172 35 596.3 0 35 173 29 596.3 0 29 174 25 596.3 0 25 175 34 596.3 0 34 176 65 596.3 0 65 177 36 596.3 0 36 178 48 596.3 0 48 179 46 596.3 0 46 180 48 596.3 0 48 181 51 596.3 0 51 182 35 596.3 0 35 183 47 596.3 0 47 184 44 596.3 0 44 185 43 596.3 0 43 186 43 596.3 0 43 187 36 596.3 0 36 188 28 596.3 0 28 189 38 596.3 0 38 190 48 596.3 0 48 191 53 596.3 0 53 192 82 596.3 0 82 193 32 596.3 0 32 194 43 596.3 0 43 195 41 596.3 0 41 196 49 596.3 0 49 197 44 596.3 0 44 198 43 596.3 0 43 199 29 596.3 0 29 200 49 596.3 0 49 201 72 596.3 0 72 202 87 596.3 0 87 203 85 596.3 0 85 204 50 596.3 0 50 205 47 596.3 0 47 206 39 596.3 0 39 207 62 596.3 0 62 208 82 596.3 0 82 209 101 596.3 0 101 210 105 596.3 0 105 211 77 596.3 0 77 212 29 596.3 0 29 213 31 596.3 0 31 214 34 596.3 0 34 215 26 596.3 0 26 216 73 596.3 0 73 217 47 596.3 0 47 218 52 596.3 0 52 219 42 596.3 0 42 220 46 596.3 0 46 221 37 596.3 0 37 222 58 596.3 0 58 223 104 596.3 0 104 224 74 596.3 0 74 225 55 596.3 0 55 226 57 596.3 0 57 227 55 596.3 0 55 228 33 596.3 0 33 229 45 596.3 0 45 230 38 596.3 0 38 231 34 596.3 0 34 232 42 596.3 0 42 233 38 596.3 0 38 234 33 596.3 0 33 235 54 596.3 0 54 236 44 596.3 0 44 237 50 596.3 0 50 238 53 596.3 0 53 239 110 596.3 0 110 240 64 596.3 0 64 241 59 596.3 0 59 242 235 596.3 0 235 243 108 596.3 0 108 244 164 596.3 0 164 245 117 596.3 0 117 246 191 596.3 0 191 247 38 596.3 0 38 248 60 596.3 0 60 249 49 596.3 0 49 250 41 596.3 0 41 251 45 596.3 0 45 252 91 596.3 0 91 253 252 596.3 0 252 254 175 596.3 0 175 255 424 596.3 0 424 256 71 596.3 0 71 257 212 596.3 0 212 258 986 596.3 0 986 259 719 596.3 0 719 260 765 596.3 0 765 261 47 596.3 0 47 262 55 596.3 0 55 263 91 596.3 0 91 264 112 596.3 0 112 265 130 596.3 0 130 266 41 596.3 0 41 267 21 596.3 0 21 268 36 596.3 0 36 269 48 596.3 0 48 270 77 596.3 0 77 271 142 596.3 0 142 272 113 596.3 0 113 273 207 596.3 0 207 274 50 596.3 0 50 275 52 596.3 0 52 276 42 596.3 0 42 277 53 596.3 0 53 278 49 596.3 0 49 279 48 596.3 0 48 280 34 596.3 0 34 281 68 596.3 0 68 282 65 596.3 0 65 283 72 596.3 0 72 284 77 596.3 0 77 285 68 596.3 0 68 286 66 596.3 0 66 287 46 596.3 0 46 288 40 596.3 0 40 289 39 596.3 0 39 290 102 596.3 0 102 291 226 596.3 0 226 292 138 596.3 0 138 293 48 596.3 0 48 294 44 596.3 0 44 295 42 596.3 0 42 296 48 596.3 0 48 297 47 596.3 0 47 298 80 596.3 0 80 299 60 596.3 0 60 300 52 596.3 0 52 301 53 596.3 0 53 Finished in 5.85 s (51 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 115,755 Reads with adapters: 59,018 (51.0%) Reads that were too short: 28 (0.0%) Reads written (passing filters): 58,990 (51.0%) Total basepairs processed: 33,086,136 bp Total written (filtered): 15,987,977 bp (48.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 59018 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 13 0.0 3 0 0 13 19 435 0.0 3 0 421 12 2 20 57227 0.0 4 56161 1029 33 2 2 21 141 0.0 4 21 120 22 19 0.0 4 8 0 9 2 23 46 0.0 4 0 0 0 41 5 24 1 0.0 4 0 0 0 0 1 26 14 0.0 4 0 0 0 0 14 55 1 0.0 4 0 1 80 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 125 7 0.0 4 0 0 0 0 7 126 7 0.0 4 0 0 0 0 7 127 1076 0.0 4 0 0 0 0 1076 128 3 0.0 4 0 0 0 0 3 135 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 6 0.0 4 0 0 0 1 5 229 2 0.0 4 0 0 0 1 1 232 3 0.0 4 0 0 0 0 3 235 2 0.0 4 0 0 0 0 2 282 2 0.0 4 0 0 0 0 2 293 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.07 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 58,990 Reads with adapters: 4,617 (7.8%) Reads written (passing filters): 58,990 (100.0%) Total basepairs processed: 15,987,977 bp Total written (filtered): 15,934,628 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4617 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.9% C: 3.3% G: 93.1% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 35 921.7 0 35 4 41 230.4 0 41 5 2 57.6 0 2 6 1 14.4 0 1 8 3 0.9 0 3 9 162 0.2 0 6 156 10 692 0.1 1 34 658 11 3638 0.0 1 27 3611 12 12 0.0 1 5 7 14 1 0.0 1 0 1 15 1 0.0 1 0 1 48 1 0.0 1 1 55 1 0.0 1 1 57 1 0.0 1 0 1 85 1 0.0 1 0 1 123 10 0.0 1 10 151 4 0.0 1 1 3 180 1 0.0 1 0 1 209 2 0.0 1 2 224 7 0.0 1 7 226 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.