This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 63.52 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 81,001 (3.3%) Reads that were too short: 16,227 (0.7%) Reads written (passing filters): 64,774 (2.7%) Total basepairs processed: 735,190,694 bp Total written (filtered): 17,325,547 bp (2.4%) === Adapter 1 === Sequence: TGGCGG; Type: regular 5'; Length: 6; Trimmed: 81001 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2303 596.3 0 2303 7 21060 596.3 0 21060 8 577 596.3 0 577 9 25473 596.3 0 25473 10 61 596.3 0 61 11 33 596.3 0 33 12 41 596.3 0 41 13 44 596.3 0 44 14 36 596.3 0 36 15 43 596.3 0 43 16 27 596.3 0 27 17 40 596.3 0 40 18 64 596.3 0 64 19 54 596.3 0 54 20 31 596.3 0 31 21 31 596.3 0 31 22 42 596.3 0 42 23 38 596.3 0 38 24 35 596.3 0 35 25 29 596.3 0 29 26 27 596.3 0 27 27 48 596.3 0 48 28 36 596.3 0 36 29 25 596.3 0 25 30 37 596.3 0 37 31 39 596.3 0 39 32 26 596.3 0 26 33 51 596.3 0 51 34 37 596.3 0 37 35 40 596.3 0 40 36 39 596.3 0 39 37 48 596.3 0 48 38 35 596.3 0 35 39 35 596.3 0 35 40 38 596.3 0 38 41 45 596.3 0 45 42 36 596.3 0 36 43 38 596.3 0 38 44 35 596.3 0 35 45 46 596.3 0 46 46 43 596.3 0 43 47 159 596.3 0 159 48 122 596.3 0 122 49 215 596.3 0 215 50 55 596.3 0 55 51 40 596.3 0 40 52 41 596.3 0 41 53 44 596.3 0 44 54 53 596.3 0 53 55 51 596.3 0 51 56 54 596.3 0 54 57 49 596.3 0 49 58 56 596.3 0 56 59 97 596.3 0 97 60 86 596.3 0 86 61 119 596.3 0 119 62 44 596.3 0 44 63 73 596.3 0 73 64 41 596.3 0 41 65 34 596.3 0 34 66 32 596.3 0 32 67 35 596.3 0 35 68 41 596.3 0 41 69 49 596.3 0 49 70 38 596.3 0 38 71 45 596.3 0 45 72 88 596.3 0 88 73 42 596.3 0 42 74 44 596.3 0 44 75 46 596.3 0 46 76 52 596.3 0 52 77 108 596.3 0 108 78 97 596.3 0 97 79 87 596.3 0 87 80 44 596.3 0 44 81 39 596.3 0 39 82 57 596.3 0 57 83 48 596.3 0 48 84 209 596.3 0 209 85 285 596.3 0 285 86 270 596.3 0 270 87 37 596.3 0 37 88 26 596.3 0 26 89 42 596.3 0 42 90 34 596.3 0 34 91 47 596.3 0 47 92 33 596.3 0 33 93 33 596.3 0 33 94 47 596.3 0 47 95 36 596.3 0 36 96 39 596.3 0 39 97 40 596.3 0 40 98 28 596.3 0 28 99 52 596.3 0 52 100 37 596.3 0 37 101 32 596.3 0 32 102 40 596.3 0 40 103 34 596.3 0 34 104 36 596.3 0 36 105 44 596.3 0 44 106 64 596.3 0 64 107 32 596.3 0 32 108 46 596.3 0 46 109 44 596.3 0 44 110 43 596.3 0 43 111 60 596.3 0 60 112 625 596.3 0 625 113 154 596.3 0 154 114 386 596.3 0 386 115 31 596.3 0 31 116 42 596.3 0 42 117 38 596.3 0 38 118 54 596.3 0 54 119 46 596.3 0 46 120 38 596.3 0 38 121 43 596.3 0 43 122 30 596.3 0 30 123 30 596.3 0 30 124 29 596.3 0 29 125 42 596.3 0 42 126 51 596.3 0 51 127 81 596.3 0 81 128 148 596.3 0 148 129 129 596.3 0 129 130 49 596.3 0 49 131 81 596.3 0 81 132 141 596.3 0 141 133 251 596.3 0 251 134 254 596.3 0 254 135 167 596.3 0 167 136 74 596.3 0 74 137 56 596.3 0 56 138 65 596.3 0 65 139 61 596.3 0 61 140 90 596.3 0 90 141 1065 596.3 0 1065 142 1085 596.3 0 1085 143 956 596.3 0 956 144 44 596.3 0 44 145 45 596.3 0 45 146 39 596.3 0 39 147 68 596.3 0 68 148 48 596.3 0 48 149 64 596.3 0 64 150 39 596.3 0 39 151 50 596.3 0 50 152 132 596.3 0 132 153 187 596.3 0 187 154 172 596.3 0 172 155 65 596.3 0 65 156 49 596.3 0 49 157 39 596.3 0 39 158 39 596.3 0 39 159 43 596.3 0 43 160 35 596.3 0 35 161 35 596.3 0 35 162 46 596.3 0 46 163 38 596.3 0 38 164 51 596.3 0 51 165 45 596.3 0 45 166 47 596.3 0 47 167 52 596.3 0 52 168 35 596.3 0 35 169 52 596.3 0 52 170 34 596.3 0 34 171 38 596.3 0 38 172 64 596.3 0 64 173 49 596.3 0 49 174 68 596.3 0 68 175 39 596.3 0 39 176 19 596.3 0 19 177 28 596.3 0 28 178 43 596.3 0 43 179 40 596.3 0 40 180 49 596.3 0 49 181 35 596.3 0 35 182 52 596.3 0 52 183 64 596.3 0 64 184 50 596.3 0 50 185 91 596.3 0 91 186 97 596.3 0 97 187 309 596.3 0 309 188 39 596.3 0 39 189 48 596.3 0 48 190 43 596.3 0 43 191 66 596.3 0 66 192 44 596.3 0 44 193 65 596.3 0 65 194 26 596.3 0 26 195 34 596.3 0 34 196 38 596.3 0 38 197 39 596.3 0 39 198 36 596.3 0 36 199 36 596.3 0 36 200 29 596.3 0 29 201 50 596.3 0 50 202 52 596.3 0 52 203 36 596.3 0 36 204 38 596.3 0 38 205 44 596.3 0 44 206 40 596.3 0 40 207 29 596.3 0 29 208 34 596.3 0 34 209 46 596.3 0 46 210 41 596.3 0 41 211 46 596.3 0 46 212 67 596.3 0 67 213 65 596.3 0 65 214 59 596.3 0 59 215 45 596.3 0 45 216 69 596.3 0 69 217 58 596.3 0 58 218 56 596.3 0 56 219 61 596.3 0 61 220 65 596.3 0 65 221 37 596.3 0 37 222 29 596.3 0 29 223 32 596.3 0 32 224 57 596.3 0 57 225 53 596.3 0 53 226 82 596.3 0 82 227 54 596.3 0 54 228 108 596.3 0 108 229 125 596.3 0 125 230 172 596.3 0 172 231 92 596.3 0 92 232 85 596.3 0 85 233 54 596.3 0 54 234 59 596.3 0 59 235 231 596.3 0 231 236 405 596.3 0 405 237 363 596.3 0 363 238 247 596.3 0 247 239 129 596.3 0 129 240 66 596.3 0 66 241 44 596.3 0 44 242 44 596.3 0 44 243 49 596.3 0 49 244 50 596.3 0 50 245 89 596.3 0 89 246 124 596.3 0 124 247 141 596.3 0 141 248 126 596.3 0 126 249 78 596.3 0 78 250 88 596.3 0 88 251 103 596.3 0 103 252 138 596.3 0 138 253 135 596.3 0 135 254 128 596.3 0 128 255 112 596.3 0 112 256 391 596.3 0 391 257 403 596.3 0 403 258 225 596.3 0 225 259 50 596.3 0 50 260 137 596.3 0 137 261 116 596.3 0 116 262 170 596.3 0 170 263 108 596.3 0 108 264 33 596.3 0 33 265 30 596.3 0 30 266 70 596.3 0 70 267 106 596.3 0 106 268 47 596.3 0 47 269 47 596.3 0 47 270 63 596.3 0 63 271 43 596.3 0 43 272 87 596.3 0 87 273 816 596.3 0 816 274 655 596.3 0 655 275 230 596.3 0 230 276 84 596.3 0 84 277 31 596.3 0 31 278 56 596.3 0 56 279 63 596.3 0 63 280 63 596.3 0 63 281 64 596.3 0 64 282 71 596.3 0 71 283 78 596.3 0 78 284 183 596.3 0 183 285 211 596.3 0 211 286 158 596.3 0 158 287 200 596.3 0 200 288 167 596.3 0 167 289 91 596.3 0 91 290 84 596.3 0 84 291 107 596.3 0 107 292 102 596.3 0 102 293 110 596.3 0 110 294 87 596.3 0 87 295 114 596.3 0 114 296 176 596.3 0 176 297 187 596.3 0 187 298 1265 596.3 0 1265 299 1732 596.3 0 1732 300 1740 596.3 0 1740 301 296 596.3 0 296 Finished in 3.38 s (52 us/read; 1.15 M reads/minute). === Summary === Total reads processed: 64,774 Reads with adapters: 22,231 (34.3%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 22,214 (34.3%) Total basepairs processed: 17,325,547 bp Total written (filtered): 6,007,878 bp (34.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 22231 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 10 0.0 3 0 0 10 19 276 0.0 3 0 267 9 20 19757 0.0 4 19215 518 19 4 1 21 95 0.0 4 17 76 0 2 22 1345 0.0 4 1299 37 7 0 2 23 23 0.0 4 2 2 0 15 4 24 1 0.0 4 0 0 1 62 1 0.0 4 0 0 0 0 1 117 1 0.0 4 1 125 6 0.0 4 0 0 0 0 6 126 8 0.0 4 0 0 0 0 8 127 676 0.0 4 0 0 0 0 676 128 1 0.0 4 0 0 0 0 1 129 7 0.0 4 0 0 0 0 7 130 2 0.0 4 0 0 0 0 2 153 2 0.0 4 0 0 0 0 2 155 1 0.0 4 1 172 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 3 0.0 4 0 0 0 1 2 230 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 267 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.76 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 22,214 Reads with adapters: 635 (2.9%) Reads written (passing filters): 22,214 (100.0%) Total basepairs processed: 6,007,878 bp Total written (filtered): 5,999,170 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 635 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.0% C: 7.1% G: 82.2% T: 2.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 49 347.1 0 49 4 10 86.8 0 10 5 1 21.7 0 1 8 1 0.3 0 1 9 132 0.1 0 4 128 10 124 0.0 1 3 121 11 237 0.0 1 3 234 12 61 0.0 1 0 61 13 3 0.0 1 1 2 14 1 0.0 1 1 16 1 0.0 1 0 1 92 4 0.0 1 3 1 93 1 0.0 1 1 171 1 0.0 1 1 223 2 0.0 1 2 225 6 0.0 1 5 1 244 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.