This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGACCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.35 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 147,111 (6.0%) Reads that were too short: 44,597 (1.8%) Reads written (passing filters): 102,514 (4.2%) Total basepairs processed: 735,190,694 bp Total written (filtered): 26,177,780 bp (3.6%) === Adapter 1 === Sequence: AGACCG; Type: regular 5'; Length: 6; Trimmed: 147111 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 991 596.3 0 991 7 35560 596.3 0 35560 8 575 596.3 0 575 9 27000 596.3 0 27000 10 46 596.3 0 46 11 15 596.3 0 15 12 33 596.3 0 33 13 16 596.3 0 16 14 7 596.3 0 7 15 21 596.3 0 21 16 18 596.3 0 18 17 10 596.3 0 10 18 11 596.3 0 11 19 9 596.3 0 9 20 14 596.3 0 14 21 10 596.3 0 10 22 14 596.3 0 14 23 7 596.3 0 7 24 12 596.3 0 12 25 9 596.3 0 9 26 17 596.3 0 17 27 15 596.3 0 15 28 15 596.3 0 15 29 6 596.3 0 6 30 25 596.3 0 25 31 12 596.3 0 12 32 11 596.3 0 11 33 17 596.3 0 17 34 15 596.3 0 15 35 12 596.3 0 12 36 15 596.3 0 15 37 14 596.3 0 14 38 16 596.3 0 16 39 7 596.3 0 7 40 11 596.3 0 11 41 12 596.3 0 12 42 23 596.3 0 23 43 19 596.3 0 19 44 17 596.3 0 17 45 25 596.3 0 25 46 20 596.3 0 20 47 16 596.3 0 16 48 17 596.3 0 17 49 11 596.3 0 11 50 9 596.3 0 9 51 13 596.3 0 13 52 23 596.3 0 23 53 23 596.3 0 23 54 26 596.3 0 26 55 127 596.3 0 127 56 57 596.3 0 57 57 26 596.3 0 26 58 8 596.3 0 8 59 40 596.3 0 40 60 20 596.3 0 20 61 33 596.3 0 33 62 40 596.3 0 40 63 27 596.3 0 27 64 40 596.3 0 40 65 109 596.3 0 109 66 33 596.3 0 33 67 33 596.3 0 33 68 20 596.3 0 20 69 100 596.3 0 100 70 73 596.3 0 73 71 48 596.3 0 48 72 46 596.3 0 46 73 46 596.3 0 46 74 85 596.3 0 85 75 125 596.3 0 125 76 126 596.3 0 126 77 126 596.3 0 126 78 176 596.3 0 176 79 180 596.3 0 180 80 270 596.3 0 270 81 415 596.3 0 415 82 3810 596.3 0 3810 83 2669 596.3 0 2669 84 15639 596.3 0 15639 85 143 596.3 0 143 86 60 596.3 0 60 87 18 596.3 0 18 88 39 596.3 0 39 89 28 596.3 0 28 90 14 596.3 0 14 91 18 596.3 0 18 92 37 596.3 0 37 93 21 596.3 0 21 94 15 596.3 0 15 95 14 596.3 0 14 96 11 596.3 0 11 97 28 596.3 0 28 98 15 596.3 0 15 99 21 596.3 0 21 100 11 596.3 0 11 101 15 596.3 0 15 102 43 596.3 0 43 103 41 596.3 0 41 104 28 596.3 0 28 105 15 596.3 0 15 106 24 596.3 0 24 107 19 596.3 0 19 108 10 596.3 0 10 109 17 596.3 0 17 110 15 596.3 0 15 111 21 596.3 0 21 112 28 596.3 0 28 113 37 596.3 0 37 114 38 596.3 0 38 115 32 596.3 0 32 116 27 596.3 0 27 117 19 596.3 0 19 118 28 596.3 0 28 119 33 596.3 0 33 120 29 596.3 0 29 121 147 596.3 0 147 122 190 596.3 0 190 123 320 596.3 0 320 124 209 596.3 0 209 125 70 596.3 0 70 126 43 596.3 0 43 127 67 596.3 0 67 128 137 596.3 0 137 129 27 596.3 0 27 130 22 596.3 0 22 131 26 596.3 0 26 132 29 596.3 0 29 133 54 596.3 0 54 134 44 596.3 0 44 135 23 596.3 0 23 136 99 596.3 0 99 137 33 596.3 0 33 138 87 596.3 0 87 139 80 596.3 0 80 140 329 596.3 0 329 141 36 596.3 0 36 142 22 596.3 0 22 143 24 596.3 0 24 144 31 596.3 0 31 145 24 596.3 0 24 146 40 596.3 0 40 147 50 596.3 0 50 148 51 596.3 0 51 149 123 596.3 0 123 150 34 596.3 0 34 151 34 596.3 0 34 152 62 596.3 0 62 153 54 596.3 0 54 154 51 596.3 0 51 155 34 596.3 0 34 156 30 596.3 0 30 157 25 596.3 0 25 158 32 596.3 0 32 159 31 596.3 0 31 160 31 596.3 0 31 161 28 596.3 0 28 162 52 596.3 0 52 163 63 596.3 0 63 164 52 596.3 0 52 165 76 596.3 0 76 166 114 596.3 0 114 167 1486 596.3 0 1486 168 953 596.3 0 953 169 2069 596.3 0 2069 170 85 596.3 0 85 171 189 596.3 0 189 172 133 596.3 0 133 173 307 596.3 0 307 174 32 596.3 0 32 175 35 596.3 0 35 176 44 596.3 0 44 177 42 596.3 0 42 178 38 596.3 0 38 179 30 596.3 0 30 180 94 596.3 0 94 181 308 596.3 0 308 182 433 596.3 0 433 183 68 596.3 0 68 184 94 596.3 0 94 185 134 596.3 0 134 186 155 596.3 0 155 187 144 596.3 0 144 188 78 596.3 0 78 189 92 596.3 0 92 190 84 596.3 0 84 191 72 596.3 0 72 192 41 596.3 0 41 193 44 596.3 0 44 194 42 596.3 0 42 195 52 596.3 0 52 196 49 596.3 0 49 197 40 596.3 0 40 198 95 596.3 0 95 199 171 596.3 0 171 200 717 596.3 0 717 201 425 596.3 0 425 202 347 596.3 0 347 203 242 596.3 0 242 204 41 596.3 0 41 205 83 596.3 0 83 206 112 596.3 0 112 207 121 596.3 0 121 208 51 596.3 0 51 209 33 596.3 0 33 210 63 596.3 0 63 211 83 596.3 0 83 212 52 596.3 0 52 213 75 596.3 0 75 214 97 596.3 0 97 215 305 596.3 0 305 216 230 596.3 0 230 217 110 596.3 0 110 218 111 596.3 0 111 219 135 596.3 0 135 220 138 596.3 0 138 221 96 596.3 0 96 222 91 596.3 0 91 223 126 596.3 0 126 224 195 596.3 0 195 225 255 596.3 0 255 226 392 596.3 0 392 227 833 596.3 0 833 228 10502 596.3 0 10502 229 4278 596.3 0 4278 230 6990 596.3 0 6990 231 321 596.3 0 321 232 93 596.3 0 93 233 50 596.3 0 50 234 51 596.3 0 51 235 62 596.3 0 62 236 67 596.3 0 67 237 55 596.3 0 55 238 85 596.3 0 85 239 78 596.3 0 78 240 94 596.3 0 94 241 56 596.3 0 56 242 58 596.3 0 58 243 110 596.3 0 110 244 154 596.3 0 154 245 154 596.3 0 154 246 204 596.3 0 204 247 275 596.3 0 275 248 251 596.3 0 251 249 184 596.3 0 184 250 143 596.3 0 143 251 101 596.3 0 101 252 78 596.3 0 78 253 56 596.3 0 56 254 76 596.3 0 76 255 186 596.3 0 186 256 691 596.3 0 691 257 729 596.3 0 729 258 778 596.3 0 778 259 136 596.3 0 136 260 157 596.3 0 157 261 460 596.3 0 460 262 1340 596.3 0 1340 263 1608 596.3 0 1608 264 736 596.3 0 736 265 362 596.3 0 362 266 140 596.3 0 140 267 176 596.3 0 176 268 981 596.3 0 981 269 939 596.3 0 939 270 724 596.3 0 724 271 178 596.3 0 178 272 81 596.3 0 81 273 62 596.3 0 62 274 134 596.3 0 134 275 162 596.3 0 162 276 137 596.3 0 137 277 112 596.3 0 112 278 98 596.3 0 98 279 73 596.3 0 73 280 111 596.3 0 111 281 180 596.3 0 180 282 210 596.3 0 210 283 285 596.3 0 285 284 79 596.3 0 79 285 73 596.3 0 73 286 109 596.3 0 109 287 261 596.3 0 261 288 203 596.3 0 203 289 158 596.3 0 158 290 77 596.3 0 77 291 75 596.3 0 75 292 210 596.3 0 210 293 345 596.3 0 345 294 519 596.3 0 519 295 152 596.3 0 152 296 101 596.3 0 101 297 67 596.3 0 67 298 70 596.3 0 70 299 121 596.3 0 121 300 438 596.3 0 438 301 530 596.3 0 530 Finished in 4.58 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 102,514 Reads with adapters: 36,067 (35.2%) Reads that were too short: 151 (0.1%) Reads written (passing filters): 35,916 (35.0%) Total basepairs processed: 26,177,780 bp Total written (filtered): 9,934,974 bp (38.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 36067 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 6 0.0 2 0 0 0 6 15 22 0.0 3 0 0 13 9 16 362 0.0 3 0 287 40 35 17 35122 0.0 3 32950 1754 330 88 18 88 0.0 3 15 63 8 2 19 103 0.0 3 0 1 71 31 20 124 0.0 3 0 0 0 124 21 3 0.0 3 0 1 0 2 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 24 3 0.0 3 1 0 0 2 27 2 0.0 3 0 0 0 2 30 1 0.0 3 0 0 1 31 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 37 2 0.0 3 0 0 0 2 38 1 0.0 3 0 0 0 1 39 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 0 1 45 3 0.0 3 0 0 0 3 46 34 0.0 3 0 0 0 34 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 5 0.0 3 0 0 0 5 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 4 0.0 3 0 0 0 4 57 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 0 2 59 2 0.0 3 0 0 0 2 61 2 0.0 3 0 0 0 2 65 4 0.0 3 0 0 0 4 66 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 71 17 0.0 3 0 0 1 16 74 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 81 2 0.0 3 0 0 0 2 84 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 88 2 0.0 3 0 0 1 1 95 1 0.0 3 0 0 0 1 96 8 0.0 3 0 0 1 7 97 3 0.0 3 0 0 1 2 98 6 0.0 3 0 0 0 6 99 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 102 2 0.0 3 0 0 1 1 104 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 116 3 0.0 3 0 0 0 3 121 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 132 3 0.0 3 0 0 0 3 133 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 1 140 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 4 0.0 3 0 0 0 4 155 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 163 2 0.0 3 0 0 0 2 164 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 194 6 0.0 3 0 0 1 5 200 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 1 203 6 0.0 3 0 0 0 6 204 3 0.0 3 0 0 0 3 205 13 0.0 3 0 0 0 13 207 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 228 1 0.0 3 0 0 1 237 3 0.0 3 0 0 1 2 238 2 0.0 3 0 0 0 2 240 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 278 2 0.0 3 0 0 0 2 280 4 0.0 3 0 0 0 4 281 4 0.0 3 0 0 0 4 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 285 3 0.0 3 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.27 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 35,916 Reads with adapters: 511 (1.4%) Reads written (passing filters): 35,916 (100.0%) Total basepairs processed: 9,934,974 bp Total written (filtered): 9,933,429 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 511 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 16.8% C: 70.8% G: 11.4% T: 1.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 505 561.2 0 505 4 1 140.3 0 1 5 4 35.1 0 4 6 1 8.8 0 1