This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.56 s (28 us/read; 2.14 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 1,126,430 (46.1%) Reads that were too short: 26,128 (1.1%) Reads written (passing filters): 1,100,302 (45.0%) Total basepairs processed: 735,190,694 bp Total written (filtered): 300,690,779 bp (40.9%) === Adapter 1 === Sequence: AATCCT; Type: regular 5'; Length: 6; Trimmed: 1126430 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1648 596.3 0 1648 7 48987 596.3 0 48987 8 3097 596.3 0 3097 9 51483 596.3 0 51483 10 1193 596.3 0 1193 11 1872 596.3 0 1872 12 100283 596.3 0 100283 13 2812 596.3 0 2812 14 476 596.3 0 476 15 1164 596.3 0 1164 16 422 596.3 0 422 17 700 596.3 0 700 18 2739 596.3 0 2739 19 838 596.3 0 838 20 965 596.3 0 965 21 324 596.3 0 324 22 517 596.3 0 517 23 1499 596.3 0 1499 24 1883 596.3 0 1883 25 3225 596.3 0 3225 26 5068 596.3 0 5068 27 5259 596.3 0 5259 28 10343 596.3 0 10343 29 21315 596.3 0 21315 30 282530 596.3 0 282530 31 171630 596.3 0 171630 32 363643 596.3 0 363643 33 1269 596.3 0 1269 34 115 596.3 0 115 35 197 596.3 0 197 36 413 596.3 0 413 37 378 596.3 0 378 38 30 596.3 0 30 39 183 596.3 0 183 40 27 596.3 0 27 41 17 596.3 0 17 42 16 596.3 0 16 43 19 596.3 0 19 44 25 596.3 0 25 45 50 596.3 0 50 46 16 596.3 0 16 47 38 596.3 0 38 48 16 596.3 0 16 49 18 596.3 0 18 50 21 596.3 0 21 51 25 596.3 0 25 52 27 596.3 0 27 53 40 596.3 0 40 54 254 596.3 0 254 55 584 596.3 0 584 56 462 596.3 0 462 57 12 596.3 0 12 58 18 596.3 0 18 59 14 596.3 0 14 60 14 596.3 0 14 61 17 596.3 0 17 62 8 596.3 0 8 63 12 596.3 0 12 64 11 596.3 0 11 65 18 596.3 0 18 66 12 596.3 0 12 67 15 596.3 0 15 68 9 596.3 0 9 69 34 596.3 0 34 70 39 596.3 0 39 71 42 596.3 0 42 72 25 596.3 0 25 73 28 596.3 0 28 74 16 596.3 0 16 75 18 596.3 0 18 76 16 596.3 0 16 77 12 596.3 0 12 78 7 596.3 0 7 79 16 596.3 0 16 80 28 596.3 0 28 81 35 596.3 0 35 82 35 596.3 0 35 83 61 596.3 0 61 84 17 596.3 0 17 85 30 596.3 0 30 86 23 596.3 0 23 87 56 596.3 0 56 88 157 596.3 0 157 89 43 596.3 0 43 90 20 596.3 0 20 91 20 596.3 0 20 92 32 596.3 0 32 93 12 596.3 0 12 94 28 596.3 0 28 95 12 596.3 0 12 96 27 596.3 0 27 97 36 596.3 0 36 98 52 596.3 0 52 99 21 596.3 0 21 100 26 596.3 0 26 101 50 596.3 0 50 102 201 596.3 0 201 103 303 596.3 0 303 104 576 596.3 0 576 105 776 596.3 0 776 106 883 596.3 0 883 107 469 596.3 0 469 108 15 596.3 0 15 109 15 596.3 0 15 110 24 596.3 0 24 111 28 596.3 0 28 112 31 596.3 0 31 113 43 596.3 0 43 114 196 596.3 0 196 115 28 596.3 0 28 116 11 596.3 0 11 117 24 596.3 0 24 118 21 596.3 0 21 119 26 596.3 0 26 120 22 596.3 0 22 121 15 596.3 0 15 122 35 596.3 0 35 123 70 596.3 0 70 124 265 596.3 0 265 125 339 596.3 0 339 126 174 596.3 0 174 127 45 596.3 0 45 128 21 596.3 0 21 129 31 596.3 0 31 130 16 596.3 0 16 131 20 596.3 0 20 132 19 596.3 0 19 133 31 596.3 0 31 134 32 596.3 0 32 135 31 596.3 0 31 136 32 596.3 0 32 137 268 596.3 0 268 138 326 596.3 0 326 139 366 596.3 0 366 140 53 596.3 0 53 141 179 596.3 0 179 142 84 596.3 0 84 143 95 596.3 0 95 144 34 596.3 0 34 145 26 596.3 0 26 146 33 596.3 0 33 147 44 596.3 0 44 148 49 596.3 0 49 149 96 596.3 0 96 150 39 596.3 0 39 151 49 596.3 0 49 152 26 596.3 0 26 153 13 596.3 0 13 154 28 596.3 0 28 155 29 596.3 0 29 156 30 596.3 0 30 157 28 596.3 0 28 158 42 596.3 0 42 159 139 596.3 0 139 160 93 596.3 0 93 161 239 596.3 0 239 162 50 596.3 0 50 163 65 596.3 0 65 164 34 596.3 0 34 165 33 596.3 0 33 166 69 596.3 0 69 167 48 596.3 0 48 168 205 596.3 0 205 169 26 596.3 0 26 170 21 596.3 0 21 171 35 596.3 0 35 172 24 596.3 0 24 173 16 596.3 0 16 174 18 596.3 0 18 175 20 596.3 0 20 176 28 596.3 0 28 177 37 596.3 0 37 178 28 596.3 0 28 179 26 596.3 0 26 180 28 596.3 0 28 181 31 596.3 0 31 182 62 596.3 0 62 183 62 596.3 0 62 184 65 596.3 0 65 185 62 596.3 0 62 186 54 596.3 0 54 187 47 596.3 0 47 188 41 596.3 0 41 189 25 596.3 0 25 190 22 596.3 0 22 191 22 596.3 0 22 192 28 596.3 0 28 193 39 596.3 0 39 194 26 596.3 0 26 195 31 596.3 0 31 196 25 596.3 0 25 197 20 596.3 0 20 198 16 596.3 0 16 199 42 596.3 0 42 200 49 596.3 0 49 201 57 596.3 0 57 202 28 596.3 0 28 203 29 596.3 0 29 204 58 596.3 0 58 205 254 596.3 0 254 206 290 596.3 0 290 207 150 596.3 0 150 208 36 596.3 0 36 209 17 596.3 0 17 210 27 596.3 0 27 211 41 596.3 0 41 212 75 596.3 0 75 213 58 596.3 0 58 214 61 596.3 0 61 215 42 596.3 0 42 216 31 596.3 0 31 217 33 596.3 0 33 218 35 596.3 0 35 219 43 596.3 0 43 220 47 596.3 0 47 221 25 596.3 0 25 222 20 596.3 0 20 223 32 596.3 0 32 224 32 596.3 0 32 225 37 596.3 0 37 226 178 596.3 0 178 227 170 596.3 0 170 228 297 596.3 0 297 229 46 596.3 0 46 230 50 596.3 0 50 231 77 596.3 0 77 232 146 596.3 0 146 233 87 596.3 0 87 234 34 596.3 0 34 235 24 596.3 0 24 236 49 596.3 0 49 237 34 596.3 0 34 238 32 596.3 0 32 239 25 596.3 0 25 240 39 596.3 0 39 241 50 596.3 0 50 242 53 596.3 0 53 243 44 596.3 0 44 244 45 596.3 0 45 245 48 596.3 0 48 246 88 596.3 0 88 247 142 596.3 0 142 248 64 596.3 0 64 249 95 596.3 0 95 250 114 596.3 0 114 251 83 596.3 0 83 252 76 596.3 0 76 253 128 596.3 0 128 254 1098 596.3 0 1098 255 1860 596.3 0 1860 256 1279 596.3 0 1279 257 954 596.3 0 954 258 284 596.3 0 284 259 298 596.3 0 298 260 2316 596.3 0 2316 261 3518 596.3 0 3518 262 3909 596.3 0 3909 263 139 596.3 0 139 264 60 596.3 0 60 265 66 596.3 0 66 266 52 596.3 0 52 267 80 596.3 0 80 268 121 596.3 0 121 269 148 596.3 0 148 270 126 596.3 0 126 271 60 596.3 0 60 272 141 596.3 0 141 273 197 596.3 0 197 274 426 596.3 0 426 275 943 596.3 0 943 276 668 596.3 0 668 277 960 596.3 0 960 278 274 596.3 0 274 279 226 596.3 0 226 280 258 596.3 0 258 281 480 596.3 0 480 282 397 596.3 0 397 283 271 596.3 0 271 284 34 596.3 0 34 285 20 596.3 0 20 286 38 596.3 0 38 287 48 596.3 0 48 288 47 596.3 0 47 289 42 596.3 0 42 290 44 596.3 0 44 291 42 596.3 0 42 292 33 596.3 0 33 293 54 596.3 0 54 294 76 596.3 0 76 295 47 596.3 0 47 296 36 596.3 0 36 297 23 596.3 0 23 298 21 596.3 0 21 299 33 596.3 0 33 300 36 596.3 0 36 301 26 596.3 0 26 Finished in 45.09 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 1,100,302 Reads with adapters: 58,330 (5.3%) Reads that were too short: 1,488 (0.1%) Reads written (passing filters): 56,842 (5.2%) Total basepairs processed: 300,690,779 bp Total written (filtered): 15,284,814 bp (5.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 58330 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 10 0.0 2 0 0 0 10 15 22 0.0 3 0 0 12 10 16 661 0.0 3 0 588 52 21 17 53289 0.0 3 50072 2738 386 93 18 164 0.0 3 10 120 15 19 19 136 0.0 3 17 4 64 51 20 90 0.0 3 1 0 0 89 21 2 0.0 3 0 0 0 2 24 1 0.0 3 0 0 0 1 25 2 0.0 3 0 0 0 2 27 1 0.0 3 0 1 28 3 0.0 3 1 0 0 2 32 4 0.0 3 2 0 0 2 33 1 0.0 3 0 0 0 1 35 2 0.0 3 1 0 0 1 38 1 0.0 3 1 40 2 0.0 3 1 0 0 1 41 2 0.0 3 0 0 0 2 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 45 20 0.0 3 0 0 0 20 46 1 0.0 3 0 0 0 1 48 3 0.0 3 1 0 0 2 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 54 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 58 3 0.0 3 0 0 0 3 59 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 62 1 0.0 3 1 63 3 0.0 3 0 0 1 2 65 4 0.0 3 0 1 0 3 67 20 0.0 3 0 0 1 19 68 13 0.0 3 1 0 0 12 69 11 0.0 3 0 0 0 11 70 8 0.0 3 0 0 0 8 71 4 0.0 3 0 0 1 3 72 1 0.0 3 0 0 0 1 73 13 0.0 3 0 0 0 13 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 2 0.0 3 0 0 0 2 78 2 0.0 3 0 0 0 2 79 2 0.0 3 0 0 1 1 82 2 0.0 3 0 0 0 2 84 2 0.0 3 1 0 0 1 86 1 0.0 3 0 0 0 1 87 3 0.0 3 0 1 0 2 89 3 0.0 3 0 0 0 3 90 9 0.0 3 0 0 0 9 91 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 94 3 0.0 3 0 0 0 3 95 3 0.0 3 0 0 0 3 99 1 0.0 3 0 0 1 101 2 0.0 3 0 0 0 2 102 6 0.0 3 0 0 0 6 104 5 0.0 3 0 0 0 5 106 1 0.0 3 0 0 0 1 110 4 0.0 3 0 0 0 4 111 3 0.0 3 0 1 0 2 112 224 0.0 3 0 0 3 221 113 7 0.0 3 0 0 0 7 114 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 10 0.0 3 0 0 2 8 118 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 4 0.0 3 0 0 0 4 124 1 0.0 3 0 0 0 1 125 2 0.0 3 0 1 0 1 126 134 0.0 3 0 0 2 132 127 36 0.0 3 0 0 0 36 128 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 133 29 0.0 3 0 0 0 29 134 1 0.0 3 0 0 1 135 2 0.0 3 0 0 0 2 136 4 0.0 3 0 0 0 4 137 15 0.0 3 0 0 0 15 138 3 0.0 3 0 0 0 3 139 3 0.0 3 0 0 0 3 140 6 0.0 3 0 0 0 6 141 24 0.0 3 0 0 0 24 142 1 0.0 3 0 0 0 1 149 2 0.0 3 0 0 0 2 150 9 0.0 3 0 0 0 9 151 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 155 4 0.0 3 0 0 0 4 156 36 0.0 3 0 0 0 36 157 8 0.0 3 0 0 0 8 158 6 0.0 3 0 0 0 6 159 11 0.0 3 0 0 0 11 160 13 0.0 3 0 0 0 13 161 356 0.0 3 0 0 4 352 162 530 0.0 3 1 0 12 517 163 160 0.0 3 0 0 1 159 164 51 0.0 3 0 0 1 50 165 63 0.0 3 0 0 4 59 166 14 0.0 3 0 0 2 12 167 2 0.0 3 0 0 0 2 168 2 0.0 3 0 0 0 2 169 40 0.0 3 0 0 1 39 170 11 0.0 3 0 0 0 11 171 89 0.0 3 0 0 4 85 172 15 0.0 3 0 0 2 13 173 10 0.0 3 0 0 0 10 174 5 0.0 3 0 0 0 5 175 1 0.0 3 0 0 0 1 176 9 0.0 3 0 0 0 9 177 5 0.0 3 0 0 0 5 178 1 0.0 3 0 0 0 1 179 8 0.0 3 0 0 0 8 180 29 0.0 3 0 0 1 28 181 220 0.0 3 0 0 0 220 182 107 0.0 3 0 0 3 104 183 17 0.0 3 0 0 0 17 184 31 0.0 3 0 0 2 29 185 23 0.0 3 0 0 1 22 186 2 0.0 3 0 0 0 2 187 12 0.0 3 0 0 0 12 188 11 0.0 3 0 0 2 9 189 3 0.0 3 0 0 0 3 190 2 0.0 3 0 0 0 2 192 2 0.0 3 0 0 0 2 193 11 0.0 3 0 0 0 11 194 12 0.0 3 0 0 0 12 195 4 0.0 3 0 1 0 3 196 2 0.0 3 0 0 0 2 197 1 0.0 3 0 0 0 1 198 76 0.0 3 0 0 1 75 199 6 0.0 3 0 0 0 6 200 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 202 2 0.0 3 0 0 0 2 203 3 0.0 3 0 0 0 3 204 3 0.0 3 0 0 0 3 205 18 0.0 3 0 0 1 17 206 4 0.0 3 0 0 0 4 207 13 0.0 3 0 0 0 13 208 2 0.0 3 0 0 0 2 209 2 0.0 3 0 0 1 1 211 2 0.0 3 0 0 0 2 212 9 0.0 3 0 0 1 8 213 14 0.0 3 0 0 1 13 214 13 0.0 3 0 0 1 12 215 29 0.0 3 0 0 0 29 216 40 0.0 3 0 0 4 36 217 2 0.0 3 0 0 0 2 218 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 221 4 0.0 3 0 0 0 4 222 2 0.0 3 0 0 0 2 224 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 229 2 0.0 3 0 0 0 2 231 1 0.0 3 0 0 0 1 232 20 0.0 3 0 0 1 19 233 8 0.0 3 0 0 1 7 234 1 0.0 3 0 0 0 1 237 2 0.0 3 0 0 0 2 238 2 0.0 3 0 0 0 2 239 1 0.0 3 0 0 0 1 241 18 0.0 3 0 0 0 18 242 5 0.0 3 0 0 0 5 243 1 0.0 3 0 0 0 1 245 7 0.0 3 0 0 0 7 246 1 0.0 3 0 0 0 1 247 25 0.0 3 0 0 1 24 248 3 0.0 3 0 0 0 3 249 2 0.0 3 0 0 0 2 250 1 0.0 3 0 0 0 1 251 2 0.0 3 0 0 0 2 253 2 0.0 3 0 0 0 2 255 18 0.0 3 0 0 0 18 256 84 0.0 3 0 0 0 84 257 89 0.0 3 0 0 2 87 258 136 0.0 3 0 0 2 134 259 104 0.0 3 0 0 5 99 260 16 0.0 3 0 0 0 16 261 4 0.0 3 0 0 1 3 262 8 0.0 3 0 0 0 8 263 1 0.0 3 0 0 1 264 7 0.0 3 0 0 0 7 265 26 0.0 3 0 0 0 26 266 21 0.0 3 0 0 0 21 267 5 0.0 3 0 0 0 5 268 8 0.0 3 0 0 0 8 269 7 0.0 3 0 0 0 7 270 3 0.0 3 0 0 0 3 271 9 0.0 3 0 0 1 8 272 6 0.0 3 0 0 0 6 273 27 0.0 3 0 0 0 27 274 68 0.0 3 0 0 3 65 275 37 0.0 3 0 0 0 37 276 22 0.0 3 0 0 1 21 277 59 0.0 3 0 0 3 56 278 25 0.0 3 0 0 1 24 279 9 0.0 3 0 0 0 9 280 8 0.0 3 0 0 0 8 281 10 0.0 3 0 0 0 10 282 38 0.0 3 0 0 1 37 283 58 0.0 3 0 0 4 54 284 10 0.0 3 0 0 0 10 286 3 0.0 3 0 0 0 3 287 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.99 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 56,842 Reads with adapters: 1,398 (2.5%) Reads written (passing filters): 56,842 (100.0%) Total basepairs processed: 15,284,814 bp Total written (filtered): 15,280,562 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1398 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 53.6% C: 13.8% G: 29.8% T: 2.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1345 888.2 0 1345 4 49 222.0 0 49 5 3 55.5 0 3 6 1 13.9 0 1