This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.71 s (27 us/read; 2.20 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 350,507 (14.4%) Reads that were too short: 21,723 (0.9%) Reads written (passing filters): 328,784 (13.5%) Total basepairs processed: 735,190,694 bp Total written (filtered): 90,852,215 bp (12.4%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 350507 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2424 596.3 0 2424 7 2860 596.3 0 2860 8 138563 596.3 0 138563 9 124993 596.3 0 124993 10 160 596.3 0 160 11 29 596.3 0 29 12 24 596.3 0 24 13 28 596.3 0 28 14 28 596.3 0 28 15 25 596.3 0 25 16 30 596.3 0 30 17 21 596.3 0 21 18 22 596.3 0 22 19 34 596.3 0 34 20 21 596.3 0 21 21 23 596.3 0 23 22 17 596.3 0 17 23 24 596.3 0 24 24 65 596.3 0 65 25 32 596.3 0 32 26 17 596.3 0 17 27 9 596.3 0 9 28 20 596.3 0 20 29 18 596.3 0 18 30 17 596.3 0 17 31 27 596.3 0 27 32 38 596.3 0 38 33 29 596.3 0 29 34 16 596.3 0 16 35 41 596.3 0 41 36 22 596.3 0 22 37 25 596.3 0 25 38 30 596.3 0 30 39 31 596.3 0 31 40 22 596.3 0 22 41 29 596.3 0 29 42 111 596.3 0 111 43 22 596.3 0 22 44 29 596.3 0 29 45 24 596.3 0 24 46 19 596.3 0 19 47 16 596.3 0 16 48 12 596.3 0 12 49 33 596.3 0 33 50 34 596.3 0 34 51 58 596.3 0 58 52 126 596.3 0 126 53 51 596.3 0 51 54 786 596.3 0 786 55 101 596.3 0 101 56 50 596.3 0 50 57 124 596.3 0 124 58 52 596.3 0 52 59 65 596.3 0 65 60 42 596.3 0 42 61 47 596.3 0 47 62 77 596.3 0 77 63 100 596.3 0 100 64 150 596.3 0 150 65 241 596.3 0 241 66 254 596.3 0 254 67 486 596.3 0 486 68 1019 596.3 0 1019 69 12282 596.3 0 12282 70 11545 596.3 0 11545 71 9033 596.3 0 9033 72 58 596.3 0 58 73 22 596.3 0 22 74 26 596.3 0 26 75 37 596.3 0 37 76 51 596.3 0 51 77 74 596.3 0 74 78 56 596.3 0 56 79 38 596.3 0 38 80 298 596.3 0 298 81 131 596.3 0 131 82 217 596.3 0 217 83 38 596.3 0 38 84 21 596.3 0 21 85 22 596.3 0 22 86 45 596.3 0 45 87 58 596.3 0 58 88 33 596.3 0 33 89 42 596.3 0 42 90 60 596.3 0 60 91 61 596.3 0 61 92 28 596.3 0 28 93 49 596.3 0 49 94 43 596.3 0 43 95 36 596.3 0 36 96 99 596.3 0 99 97 81 596.3 0 81 98 40 596.3 0 40 99 63 596.3 0 63 100 518 596.3 0 518 101 807 596.3 0 807 102 1453 596.3 0 1453 103 118 596.3 0 118 104 39 596.3 0 39 105 34 596.3 0 34 106 26 596.3 0 26 107 46 596.3 0 46 108 39 596.3 0 39 109 47 596.3 0 47 110 46 596.3 0 46 111 59 596.3 0 59 112 74 596.3 0 74 113 74 596.3 0 74 114 53 596.3 0 53 115 51 596.3 0 51 116 50 596.3 0 50 117 70 596.3 0 70 118 72 596.3 0 72 119 77 596.3 0 77 120 36 596.3 0 36 121 45 596.3 0 45 122 74 596.3 0 74 123 70 596.3 0 70 124 85 596.3 0 85 125 57 596.3 0 57 126 57 596.3 0 57 127 42 596.3 0 42 128 46 596.3 0 46 129 35 596.3 0 35 130 70 596.3 0 70 131 123 596.3 0 123 132 535 596.3 0 535 133 637 596.3 0 637 134 574 596.3 0 574 135 71 596.3 0 71 136 320 596.3 0 320 137 304 596.3 0 304 138 280 596.3 0 280 139 117 596.3 0 117 140 81 596.3 0 81 141 61 596.3 0 61 142 54 596.3 0 54 143 75 596.3 0 75 144 98 596.3 0 98 145 62 596.3 0 62 146 61 596.3 0 61 147 68 596.3 0 68 148 81 596.3 0 81 149 58 596.3 0 58 150 72 596.3 0 72 151 75 596.3 0 75 152 124 596.3 0 124 153 104 596.3 0 104 154 143 596.3 0 143 155 89 596.3 0 89 156 79 596.3 0 79 157 130 596.3 0 130 158 199 596.3 0 199 159 201 596.3 0 201 160 277 596.3 0 277 161 394 596.3 0 394 162 91 596.3 0 91 163 127 596.3 0 127 164 90 596.3 0 90 165 60 596.3 0 60 166 149 596.3 0 149 167 83 596.3 0 83 168 115 596.3 0 115 169 170 596.3 0 170 170 595 596.3 0 595 171 97 596.3 0 97 172 146 596.3 0 146 173 54 596.3 0 54 174 80 596.3 0 80 175 85 596.3 0 85 176 297 596.3 0 297 177 491 596.3 0 491 178 954 596.3 0 954 179 1183 596.3 0 1183 180 351 596.3 0 351 181 144 596.3 0 144 182 493 596.3 0 493 183 730 596.3 0 730 184 1046 596.3 0 1046 185 356 596.3 0 356 186 155 596.3 0 155 187 145 596.3 0 145 188 131 596.3 0 131 189 163 596.3 0 163 190 148 596.3 0 148 191 117 596.3 0 117 192 104 596.3 0 104 193 70 596.3 0 70 194 74 596.3 0 74 195 71 596.3 0 71 196 130 596.3 0 130 197 212 596.3 0 212 198 296 596.3 0 296 199 245 596.3 0 245 200 113 596.3 0 113 201 76 596.3 0 76 202 483 596.3 0 483 203 739 596.3 0 739 204 745 596.3 0 745 205 601 596.3 0 601 206 76 596.3 0 76 207 65 596.3 0 65 208 64 596.3 0 64 209 78 596.3 0 78 210 87 596.3 0 87 211 177 596.3 0 177 212 127 596.3 0 127 213 102 596.3 0 102 214 91 596.3 0 91 215 71 596.3 0 71 216 90 596.3 0 90 217 114 596.3 0 114 218 150 596.3 0 150 219 144 596.3 0 144 220 77 596.3 0 77 221 90 596.3 0 90 222 282 596.3 0 282 223 697 596.3 0 697 224 639 596.3 0 639 225 440 596.3 0 440 226 148 596.3 0 148 227 141 596.3 0 141 228 150 596.3 0 150 229 144 596.3 0 144 230 84 596.3 0 84 231 63 596.3 0 63 232 78 596.3 0 78 233 77 596.3 0 77 234 80 596.3 0 80 235 78 596.3 0 78 236 66 596.3 0 66 237 98 596.3 0 98 238 77 596.3 0 77 239 102 596.3 0 102 240 55 596.3 0 55 241 99 596.3 0 99 242 321 596.3 0 321 243 482 596.3 0 482 244 597 596.3 0 597 245 363 596.3 0 363 246 113 596.3 0 113 247 73 596.3 0 73 248 51 596.3 0 51 249 55 596.3 0 55 250 66 596.3 0 66 251 131 596.3 0 131 252 165 596.3 0 165 253 212 596.3 0 212 254 104 596.3 0 104 255 336 596.3 0 336 256 284 596.3 0 284 257 306 596.3 0 306 258 137 596.3 0 137 259 150 596.3 0 150 260 106 596.3 0 106 261 118 596.3 0 118 262 141 596.3 0 141 263 139 596.3 0 139 264 205 596.3 0 205 265 540 596.3 0 540 266 342 596.3 0 342 267 306 596.3 0 306 268 125 596.3 0 125 269 374 596.3 0 374 270 499 596.3 0 499 271 879 596.3 0 879 272 402 596.3 0 402 273 435 596.3 0 435 274 247 596.3 0 247 275 142 596.3 0 142 276 201 596.3 0 201 277 313 596.3 0 313 278 359 596.3 0 359 279 309 596.3 0 309 280 98 596.3 0 98 281 88 596.3 0 88 282 89 596.3 0 89 283 165 596.3 0 165 284 169 596.3 0 169 285 204 596.3 0 204 286 182 596.3 0 182 287 126 596.3 0 126 288 155 596.3 0 155 289 88 596.3 0 88 290 87 596.3 0 87 291 255 596.3 0 255 292 476 596.3 0 476 293 306 596.3 0 306 294 230 596.3 0 230 295 117 596.3 0 117 296 77 596.3 0 77 297 145 596.3 0 145 298 118 596.3 0 118 299 156 596.3 0 156 300 198 596.3 0 198 301 297 596.3 0 297 Finished in 14.03 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 328,784 Reads with adapters: 139,530 (42.4%) Reads that were too short: 407 (0.1%) Reads written (passing filters): 139,123 (42.3%) Total basepairs processed: 90,852,215 bp Total written (filtered): 38,352,752 bp (42.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 139530 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 19 0.0 2 0 0 0 19 15 145 0.0 3 0 0 91 54 16 1401 0.0 3 0 1214 115 72 17 135289 0.0 3 126191 7522 1288 288 18 452 0.0 3 27 348 55 22 19 476 0.0 3 3 11 263 199 20 1041 0.0 3 727 61 9 244 21 4 0.0 3 0 3 0 1 22 4 0.0 3 2 0 0 2 23 2 0.0 3 1 0 0 1 28 1 0.0 3 0 0 0 1 29 2 0.0 3 0 0 0 2 30 1 0.0 3 1 32 1 0.0 3 0 0 0 1 34 17 0.0 3 0 0 0 17 35 1 0.0 3 0 0 0 1 36 2 0.0 3 0 0 0 2 37 12 0.0 3 0 0 1 11 38 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 1 40 9 0.0 3 0 0 0 9 41 2 0.0 3 0 0 0 2 42 3 0.0 3 0 0 1 2 44 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 10 0.0 3 0 0 1 9 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 2 0.0 3 0 0 0 2 55 16 0.0 3 0 0 0 16 57 3 0.0 3 0 0 0 3 58 5 0.0 3 0 0 0 5 59 2 0.0 3 0 0 0 2 60 11 0.0 3 0 0 0 11 61 2 0.0 3 0 0 0 2 62 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 68 3 0.0 3 0 0 0 3 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 9 0.0 3 0 0 0 9 72 2 0.0 3 0 0 0 2 73 2 0.0 3 0 0 0 2 74 3 0.0 3 0 0 1 2 75 3 0.0 3 0 0 0 3 77 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 80 3 0.0 3 0 0 0 3 81 1 0.0 3 0 0 0 1 82 3 0.0 3 0 0 0 3 85 5 0.0 3 0 0 0 5 86 3 0.0 3 0 0 0 3 88 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 91 61 0.0 3 0 0 1 60 92 11 0.0 3 0 0 0 11 94 16 0.0 3 0 0 1 15 95 1 0.0 3 0 0 0 1 96 2 0.0 3 0 0 0 2 97 1 0.0 3 0 0 0 1 98 3 0.0 3 0 0 0 3 99 6 0.0 3 0 0 1 5 101 9 0.0 3 0 0 1 8 102 1 0.0 3 0 0 0 1 103 6 0.0 3 0 0 0 6 104 11 0.0 3 0 0 1 10 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 110 7 0.0 3 0 1 0 6 111 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 114 3 0.0 3 0 0 0 3 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 1 122 3 0.0 3 0 0 0 3 123 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 127 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 129 3 0.0 3 0 0 0 3 130 6 0.0 3 0 0 0 6 132 3 0.0 3 0 0 0 3 135 3 0.0 3 0 0 0 3 136 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 142 4 0.0 3 0 0 0 4 143 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 0 2 145 1 0.0 3 0 0 0 1 146 4 0.0 3 0 0 0 4 147 1 0.0 3 0 0 0 1 150 25 0.0 3 0 0 0 25 151 6 0.0 3 0 0 0 6 152 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 1 156 6 0.0 3 0 0 0 6 158 1 0.0 3 0 0 0 1 159 38 0.0 3 0 0 1 37 161 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 163 5 0.0 3 0 0 0 5 164 9 0.0 3 0 0 0 9 165 2 0.0 3 0 0 0 2 166 2 0.0 3 0 0 0 2 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 169 2 0.0 3 0 0 0 2 173 5 0.0 3 0 0 0 5 175 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 184 4 0.0 3 0 0 1 3 185 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 192 12 0.0 3 0 0 1 11 193 4 0.0 3 0 0 0 4 194 14 0.0 3 0 0 0 14 195 2 0.0 3 0 0 0 2 198 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 3 0.0 3 0 0 0 3 208 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 216 5 0.0 3 0 0 0 5 217 53 0.0 3 0 0 4 49 218 3 0.0 3 0 0 0 3 219 13 0.0 3 0 0 0 13 220 10 0.0 3 0 0 0 10 221 6 0.0 3 0 0 0 6 222 2 0.0 3 0 0 0 2 224 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 226 2 0.0 3 0 0 0 2 227 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 237 2 0.0 3 0 0 0 2 238 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 278 8 0.0 3 0 0 0 8 279 8 0.0 3 0 0 0 8 280 15 0.0 3 0 0 1 14 281 14 0.0 3 0 0 0 14 282 15 0.0 3 0 0 0 15 283 2 0.0 3 0 0 0 2 285 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 288 5 0.0 3 0 0 0 5 290 2 0.0 3 0 0 0 2 291 1 0.0 3 0 0 0 1 292 4 0.0 3 0 0 0 4 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.84 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 139,123 Reads with adapters: 1,290 (0.9%) Reads written (passing filters): 139,123 (100.0%) Total basepairs processed: 38,352,752 bp Total written (filtered): 38,348,688 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1290 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 27.8% C: 12.8% G: 53.3% T: 6.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1146 2173.8 0 1146 4 111 543.4 0 111 5 30 135.9 0 30 6 1 34.0 0 1 12 1 0.0 1 0 1 14 1 0.0 1 0 1