This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.07 s (27 us/read; 2.19 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 352,392 (14.4%) Reads that were too short: 10,733 (0.4%) Reads written (passing filters): 341,659 (14.0%) Total basepairs processed: 735,190,694 bp Total written (filtered): 95,139,757 bp (12.9%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 352392 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2145 596.3 0 2145 7 2136 596.3 0 2136 8 129906 596.3 0 129906 9 145130 596.3 0 145130 10 159 596.3 0 159 11 33 596.3 0 33 12 32 596.3 0 32 13 23 596.3 0 23 14 13 596.3 0 13 15 24 596.3 0 24 16 31 596.3 0 31 17 28 596.3 0 28 18 23 596.3 0 23 19 36 596.3 0 36 20 24 596.3 0 24 21 27 596.3 0 27 22 21 596.3 0 21 23 20 596.3 0 20 24 16 596.3 0 16 25 23 596.3 0 23 26 27 596.3 0 27 27 27 596.3 0 27 28 27 596.3 0 27 29 28 596.3 0 28 30 32 596.3 0 32 31 38 596.3 0 38 32 47 596.3 0 47 33 48 596.3 0 48 34 35 596.3 0 35 35 33 596.3 0 33 36 29 596.3 0 29 37 32 596.3 0 32 38 23 596.3 0 23 39 33 596.3 0 33 40 21 596.3 0 21 41 49 596.3 0 49 42 145 596.3 0 145 43 37 596.3 0 37 44 35 596.3 0 35 45 28 596.3 0 28 46 37 596.3 0 37 47 20 596.3 0 20 48 24 596.3 0 24 49 27 596.3 0 27 50 46 596.3 0 46 51 77 596.3 0 77 52 141 596.3 0 141 53 46 596.3 0 46 54 80 596.3 0 80 55 44 596.3 0 44 56 78 596.3 0 78 57 186 596.3 0 186 58 69 596.3 0 69 59 74 596.3 0 74 60 48 596.3 0 48 61 46 596.3 0 46 62 79 596.3 0 79 63 119 596.3 0 119 64 151 596.3 0 151 65 269 596.3 0 269 66 286 596.3 0 286 67 581 596.3 0 581 68 1155 596.3 0 1155 69 16965 596.3 0 16965 70 14812 596.3 0 14812 71 11972 596.3 0 11972 72 59 596.3 0 59 73 25 596.3 0 25 74 29 596.3 0 29 75 25 596.3 0 25 76 34 596.3 0 34 77 35 596.3 0 35 78 32 596.3 0 32 79 37 596.3 0 37 80 199 596.3 0 199 81 109 596.3 0 109 82 175 596.3 0 175 83 39 596.3 0 39 84 35 596.3 0 35 85 29 596.3 0 29 86 42 596.3 0 42 87 48 596.3 0 48 88 40 596.3 0 40 89 39 596.3 0 39 90 46 596.3 0 46 91 49 596.3 0 49 92 43 596.3 0 43 93 46 596.3 0 46 94 53 596.3 0 53 95 53 596.3 0 53 96 44 596.3 0 44 97 57 596.3 0 57 98 42 596.3 0 42 99 44 596.3 0 44 100 150 596.3 0 150 101 249 596.3 0 249 102 441 596.3 0 441 103 71 596.3 0 71 104 52 596.3 0 52 105 48 596.3 0 48 106 33 596.3 0 33 107 41 596.3 0 41 108 37 596.3 0 37 109 46 596.3 0 46 110 57 596.3 0 57 111 56 596.3 0 56 112 39 596.3 0 39 113 45 596.3 0 45 114 41 596.3 0 41 115 43 596.3 0 43 116 40 596.3 0 40 117 51 596.3 0 51 118 71 596.3 0 71 119 52 596.3 0 52 120 41 596.3 0 41 121 47 596.3 0 47 122 42 596.3 0 42 123 80 596.3 0 80 124 58 596.3 0 58 125 42 596.3 0 42 126 48 596.3 0 48 127 46 596.3 0 46 128 30 596.3 0 30 129 47 596.3 0 47 130 52 596.3 0 52 131 104 596.3 0 104 132 346 596.3 0 346 133 593 596.3 0 593 134 421 596.3 0 421 135 68 596.3 0 68 136 149 596.3 0 149 137 183 596.3 0 183 138 199 596.3 0 199 139 85 596.3 0 85 140 64 596.3 0 64 141 44 596.3 0 44 142 64 596.3 0 64 143 52 596.3 0 52 144 60 596.3 0 60 145 62 596.3 0 62 146 54 596.3 0 54 147 47 596.3 0 47 148 64 596.3 0 64 149 56 596.3 0 56 150 70 596.3 0 70 151 62 596.3 0 62 152 98 596.3 0 98 153 67 596.3 0 67 154 104 596.3 0 104 155 75 596.3 0 75 156 61 596.3 0 61 157 81 596.3 0 81 158 100 596.3 0 100 159 131 596.3 0 131 160 238 596.3 0 238 161 388 596.3 0 388 162 65 596.3 0 65 163 87 596.3 0 87 164 67 596.3 0 67 165 38 596.3 0 38 166 157 596.3 0 157 167 65 596.3 0 65 168 80 596.3 0 80 169 96 596.3 0 96 170 704 596.3 0 704 171 57 596.3 0 57 172 148 596.3 0 148 173 67 596.3 0 67 174 62 596.3 0 62 175 72 596.3 0 72 176 194 596.3 0 194 177 223 596.3 0 223 178 543 596.3 0 543 179 590 596.3 0 590 180 230 596.3 0 230 181 79 596.3 0 79 182 154 596.3 0 154 183 186 596.3 0 186 184 306 596.3 0 306 185 120 596.3 0 120 186 68 596.3 0 68 187 119 596.3 0 119 188 83 596.3 0 83 189 105 596.3 0 105 190 72 596.3 0 72 191 61 596.3 0 61 192 63 596.3 0 63 193 72 596.3 0 72 194 56 596.3 0 56 195 63 596.3 0 63 196 67 596.3 0 67 197 101 596.3 0 101 198 136 596.3 0 136 199 122 596.3 0 122 200 82 596.3 0 82 201 49 596.3 0 49 202 149 596.3 0 149 203 287 596.3 0 287 204 219 596.3 0 219 205 142 596.3 0 142 206 51 596.3 0 51 207 47 596.3 0 47 208 54 596.3 0 54 209 66 596.3 0 66 210 69 596.3 0 69 211 90 596.3 0 90 212 82 596.3 0 82 213 86 596.3 0 86 214 56 596.3 0 56 215 47 596.3 0 47 216 54 596.3 0 54 217 64 596.3 0 64 218 74 596.3 0 74 219 64 596.3 0 64 220 46 596.3 0 46 221 61 596.3 0 61 222 113 596.3 0 113 223 235 596.3 0 235 224 213 596.3 0 213 225 155 596.3 0 155 226 105 596.3 0 105 227 96 596.3 0 96 228 133 596.3 0 133 229 102 596.3 0 102 230 68 596.3 0 68 231 41 596.3 0 41 232 58 596.3 0 58 233 58 596.3 0 58 234 69 596.3 0 69 235 51 596.3 0 51 236 56 596.3 0 56 237 72 596.3 0 72 238 70 596.3 0 70 239 69 596.3 0 69 240 60 596.3 0 60 241 47 596.3 0 47 242 115 596.3 0 115 243 158 596.3 0 158 244 182 596.3 0 182 245 127 596.3 0 127 246 77 596.3 0 77 247 51 596.3 0 51 248 52 596.3 0 52 249 57 596.3 0 57 250 87 596.3 0 87 251 75 596.3 0 75 252 103 596.3 0 103 253 104 596.3 0 104 254 56 596.3 0 56 255 137 596.3 0 137 256 109 596.3 0 109 257 124 596.3 0 124 258 62 596.3 0 62 259 69 596.3 0 69 260 56 596.3 0 56 261 64 596.3 0 64 262 70 596.3 0 70 263 95 596.3 0 95 264 85 596.3 0 85 265 265 596.3 0 265 266 128 596.3 0 128 267 161 596.3 0 161 268 55 596.3 0 55 269 245 596.3 0 245 270 319 596.3 0 319 271 486 596.3 0 486 272 162 596.3 0 162 273 174 596.3 0 174 274 141 596.3 0 141 275 83 596.3 0 83 276 103 596.3 0 103 277 152 596.3 0 152 278 161 596.3 0 161 279 152 596.3 0 152 280 70 596.3 0 70 281 76 596.3 0 76 282 63 596.3 0 63 283 102 596.3 0 102 284 106 596.3 0 106 285 109 596.3 0 109 286 109 596.3 0 109 287 79 596.3 0 79 288 90 596.3 0 90 289 55 596.3 0 55 290 59 596.3 0 59 291 125 596.3 0 125 292 212 596.3 0 212 293 123 596.3 0 123 294 141 596.3 0 141 295 71 596.3 0 71 296 60 596.3 0 60 297 82 596.3 0 82 298 83 596.3 0 83 299 98 596.3 0 98 300 136 596.3 0 136 301 103 596.3 0 103 Finished in 15.76 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 341,659 Reads with adapters: 151,661 (44.4%) Reads that were too short: 52 (0.0%) Reads written (passing filters): 151,609 (44.4%) Total basepairs processed: 95,139,757 bp Total written (filtered): 41,008,322 bp (43.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 151661 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 51 0.0 3 0 0 50 1 19 2208 0.0 3 0 2163 36 6 3 20 146656 0.0 4 143436 3094 119 7 21 298 0.0 4 47 234 17 22 22 0.0 4 1 1 15 1 4 23 135 0.0 4 4 0 2 107 22 24 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 1 27 2 0.0 4 1 0 1 28 1 0.0 4 0 0 0 0 1 29 1 0.0 4 1 30 1 0.0 4 1 34 2 0.0 4 0 0 0 0 2 38 1 0.0 4 0 0 0 0 1 40 1 0.0 4 1 49 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 60 2 0.0 4 0 0 0 0 2 89 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 123 1 0.0 4 1 125 9 0.0 4 0 0 0 0 9 126 27 0.0 4 0 0 0 0 27 127 2133 0.0 4 0 0 0 0 2133 128 35 0.0 4 0 0 0 0 35 130 15 0.0 4 0 0 0 0 15 132 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 154 1 0.0 4 1 156 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 218 2 0.0 4 0 0 0 0 2 228 14 0.0 4 0 0 0 1 13 229 6 0.0 4 0 0 0 1 5 230 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 235 2 0.0 4 0 0 0 0 2 236 1 0.0 4 0 0 0 1 250 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 268 1 0.0 4 0 0 0 0 1 269 2 0.0 4 0 0 0 0 2 273 1 0.0 4 0 0 0 1 275 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 285 3 0.0 4 0 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.39 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 151,609 Reads with adapters: 20,735 (13.7%) Reads written (passing filters): 151,609 (100.0%) Total basepairs processed: 41,008,322 bp Total written (filtered): 40,749,332 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 20735 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.4% C: 3.1% G: 93.2% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 106 2368.9 0 106 4 29 592.2 0 29 5 7 148.1 0 7 6 5 37.0 0 5 7 1 9.3 0 1 8 3 2.3 0 3 9 9406 0.6 0 245 9161 10 8463 0.1 1 316 8147 11 2393 0.0 1 23 2370 12 23 0.0 1 0 23 13 11 0.0 1 0 11 14 2 0.0 1 0 2 18 2 0.0 1 0 2 21 1 0.0 1 1 32 1 0.0 1 0 1 34 1 0.0 1 1 61 1 0.0 1 0 1 82 1 0.0 1 1 83 1 0.0 1 0 1 85 1 0.0 1 1 91 1 0.0 1 1 105 1 0.0 1 0 1 148 1 0.0 1 1 150 3 0.0 1 0 3 162 1 0.0 1 1 163 1 0.0 1 0 1 169 5 0.0 1 5 170 1 0.0 1 1 208 1 0.0 1 1 222 1 0.0 1 1 223 216 0.0 1 203 13 224 8 0.0 1 7 1 225 3 0.0 1 2 1 226 2 0.0 1 1 1 260 31 0.0 1 27 4 261 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.