This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 167.65 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 43,726 (1.2%) Reads that were too short: 16,377 (0.5%) Reads written (passing filters): 27,349 (0.8%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 5,648,150 bp (0.5%) === Adapter 1 === Sequence: GGTTCT; Type: regular 5'; Length: 6; Trimmed: 43726 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 915 887.3 0 915 7 106 887.3 0 106 8 76 887.3 0 76 9 97 887.3 0 97 10 283 887.3 0 283 11 95 887.3 0 95 12 82 887.3 0 82 13 83 887.3 0 83 14 68 887.3 0 68 15 83 887.3 0 83 16 81 887.3 0 81 17 86 887.3 0 86 18 79 887.3 0 79 19 89 887.3 0 89 20 116 887.3 0 116 21 88 887.3 0 88 22 191 887.3 0 191 23 619 887.3 0 619 24 611 887.3 0 611 25 650 887.3 0 650 26 73 887.3 0 73 27 73 887.3 0 73 28 70 887.3 0 70 29 74 887.3 0 74 30 69 887.3 0 69 31 76 887.3 0 76 32 62 887.3 0 62 33 103 887.3 0 103 34 105 887.3 0 105 35 205 887.3 0 205 36 68 887.3 0 68 37 93 887.3 0 93 38 70 887.3 0 70 39 73 887.3 0 73 40 67 887.3 0 67 41 80 887.3 0 80 42 67 887.3 0 67 43 60 887.3 0 60 44 71 887.3 0 71 45 61 887.3 0 61 46 73 887.3 0 73 47 78 887.3 0 78 48 84 887.3 0 84 49 67 887.3 0 67 50 70 887.3 0 70 51 61 887.3 0 61 52 77 887.3 0 77 53 51 887.3 0 51 54 80 887.3 0 80 55 69 887.3 0 69 56 90 887.3 0 90 57 68 887.3 0 68 58 60 887.3 0 60 59 71 887.3 0 71 60 68 887.3 0 68 61 50 887.3 0 50 62 61 887.3 0 61 63 70 887.3 0 70 64 71 887.3 0 71 65 79 887.3 0 79 66 80 887.3 0 80 67 61 887.3 0 61 68 73 887.3 0 73 69 53 887.3 0 53 70 72 887.3 0 72 71 64 887.3 0 64 72 57 887.3 0 57 73 73 887.3 0 73 74 89 887.3 0 89 75 51 887.3 0 51 76 44 887.3 0 44 77 46 887.3 0 46 78 69 887.3 0 69 79 39 887.3 0 39 80 57 887.3 0 57 81 81 887.3 0 81 82 69 887.3 0 69 83 70 887.3 0 70 84 113 887.3 0 113 85 100 887.3 0 100 86 113 887.3 0 113 87 148 887.3 0 148 88 1860 887.3 0 1860 89 1015 887.3 0 1015 90 730 887.3 0 730 91 50 887.3 0 50 92 47 887.3 0 47 93 67 887.3 0 67 94 73 887.3 0 73 95 85 887.3 0 85 96 539 887.3 0 539 97 275 887.3 0 275 98 151 887.3 0 151 99 107 887.3 0 107 100 177 887.3 0 177 101 1660 887.3 0 1660 102 1086 887.3 0 1086 103 904 887.3 0 904 104 88 887.3 0 88 105 73 887.3 0 73 106 76 887.3 0 76 107 40 887.3 0 40 108 47 887.3 0 47 109 65 887.3 0 65 110 75 887.3 0 75 111 89 887.3 0 89 112 61 887.3 0 61 113 90 887.3 0 90 114 80 887.3 0 80 115 81 887.3 0 81 116 60 887.3 0 60 117 101 887.3 0 101 118 63 887.3 0 63 119 59 887.3 0 59 120 50 887.3 0 50 121 60 887.3 0 60 122 65 887.3 0 65 123 68 887.3 0 68 124 71 887.3 0 71 125 128 887.3 0 128 126 159 887.3 0 159 127 783 887.3 0 783 128 589 887.3 0 589 129 424 887.3 0 424 130 96 887.3 0 96 131 73 887.3 0 73 132 104 887.3 0 104 133 61 887.3 0 61 134 97 887.3 0 97 135 79 887.3 0 79 136 63 887.3 0 63 137 45 887.3 0 45 138 51 887.3 0 51 139 81 887.3 0 81 140 70 887.3 0 70 141 81 887.3 0 81 142 209 887.3 0 209 143 84 887.3 0 84 144 66 887.3 0 66 145 101 887.3 0 101 146 65 887.3 0 65 147 94 887.3 0 94 148 263 887.3 0 263 149 173 887.3 0 173 150 65 887.3 0 65 151 77 887.3 0 77 152 67 887.3 0 67 153 48 887.3 0 48 154 52 887.3 0 52 155 75 887.3 0 75 156 64 887.3 0 64 157 70 887.3 0 70 158 58 887.3 0 58 159 67 887.3 0 67 160 46 887.3 0 46 161 80 887.3 0 80 162 61 887.3 0 61 163 59 887.3 0 59 164 35 887.3 0 35 165 42 887.3 0 42 166 32 887.3 0 32 167 64 887.3 0 64 168 78 887.3 0 78 169 84 887.3 0 84 170 46 887.3 0 46 171 50 887.3 0 50 172 59 887.3 0 59 173 32 887.3 0 32 174 52 887.3 0 52 175 52 887.3 0 52 176 94 887.3 0 94 177 95 887.3 0 95 178 67 887.3 0 67 179 47 887.3 0 47 180 62 887.3 0 62 181 66 887.3 0 66 182 75 887.3 0 75 183 103 887.3 0 103 184 48 887.3 0 48 185 50 887.3 0 50 186 57 887.3 0 57 187 54 887.3 0 54 188 73 887.3 0 73 189 65 887.3 0 65 190 49 887.3 0 49 191 54 887.3 0 54 192 52 887.3 0 52 193 121 887.3 0 121 194 111 887.3 0 111 195 134 887.3 0 134 196 126 887.3 0 126 197 82 887.3 0 82 198 79 887.3 0 79 199 56 887.3 0 56 200 221 887.3 0 221 201 328 887.3 0 328 202 344 887.3 0 344 203 130 887.3 0 130 204 74 887.3 0 74 205 62 887.3 0 62 206 60 887.3 0 60 207 86 887.3 0 86 208 129 887.3 0 129 209 132 887.3 0 132 210 98 887.3 0 98 211 72 887.3 0 72 212 48 887.3 0 48 213 88 887.3 0 88 214 96 887.3 0 96 215 97 887.3 0 97 216 117 887.3 0 117 217 75 887.3 0 75 218 122 887.3 0 122 219 64 887.3 0 64 220 59 887.3 0 59 221 65 887.3 0 65 222 80 887.3 0 80 223 94 887.3 0 94 224 99 887.3 0 99 225 85 887.3 0 85 226 79 887.3 0 79 227 85 887.3 0 85 228 60 887.3 0 60 229 80 887.3 0 80 230 64 887.3 0 64 231 103 887.3 0 103 232 94 887.3 0 94 233 64 887.3 0 64 234 156 887.3 0 156 235 162 887.3 0 162 236 134 887.3 0 134 237 116 887.3 0 116 238 118 887.3 0 118 239 260 887.3 0 260 240 281 887.3 0 281 241 248 887.3 0 248 242 134 887.3 0 134 243 82 887.3 0 82 244 66 887.3 0 66 245 54 887.3 0 54 246 80 887.3 0 80 247 106 887.3 0 106 248 82 887.3 0 82 249 94 887.3 0 94 250 72 887.3 0 72 251 57 887.3 0 57 252 58 887.3 0 58 253 76 887.3 0 76 254 109 887.3 0 109 255 163 887.3 0 163 256 148 887.3 0 148 257 112 887.3 0 112 258 162 887.3 0 162 259 940 887.3 0 940 260 1450 887.3 0 1450 261 1219 887.3 0 1219 262 414 887.3 0 414 263 221 887.3 0 221 264 55 887.3 0 55 265 60 887.3 0 60 266 70 887.3 0 70 267 76 887.3 0 76 268 108 887.3 0 108 269 53 887.3 0 53 270 53 887.3 0 53 271 52 887.3 0 52 272 55 887.3 0 55 273 65 887.3 0 65 274 87 887.3 0 87 275 61 887.3 0 61 276 88 887.3 0 88 277 82 887.3 0 82 278 120 887.3 0 120 279 902 887.3 0 902 280 1052 887.3 0 1052 281 958 887.3 0 958 282 113 887.3 0 113 283 95 887.3 0 95 284 72 887.3 0 72 285 48 887.3 0 48 286 59 887.3 0 59 287 54 887.3 0 54 288 72 887.3 0 72 289 54 887.3 0 54 290 85 887.3 0 85 291 198 887.3 0 198 292 184 887.3 0 184 293 193 887.3 0 193 294 117 887.3 0 117 295 108 887.3 0 108 296 103 887.3 0 103 297 130 887.3 0 130 298 88 887.3 0 88 299 81 887.3 0 81 300 72 887.3 0 72 301 75 887.3 0 75 Finished in 1.63 s (60 us/read; 1.01 M reads/minute). === Summary === Total reads processed: 27,349 Reads with adapters: 250 (0.9%) Reads that were too short: 58 (0.2%) Reads written (passing filters): 192 (0.7%) Total basepairs processed: 5,648,150 bp Total written (filtered): 29,199 bp (0.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 250 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 17 30 0.0 3 29 0 1 18 1 0.0 3 0 1 19 1 0.0 3 0 0 0 1 20 3 0.0 3 1 2 23 1 0.0 3 0 0 0 1 24 1 0.0 3 1 25 1 0.0 3 0 0 0 1 26 3 0.0 3 0 0 0 3 34 1 0.0 3 0 0 0 1 35 2 0.0 3 0 0 0 2 38 1 0.0 3 0 0 0 1 40 9 0.0 3 0 0 9 41 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 1 45 3 0.0 3 0 0 1 2 46 3 0.0 3 0 0 0 3 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 53 4 0.0 3 0 0 0 4 55 1 0.0 3 0 0 1 57 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 64 18 0.0 3 0 0 0 18 66 9 0.0 3 0 0 0 9 67 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 75 7 0.0 3 0 0 0 7 76 3 0.0 3 0 0 0 3 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 90 3 0.0 3 0 0 0 3 92 2 0.0 3 0 0 0 2 95 2 0.0 3 0 0 0 2 96 3 0.0 3 0 0 0 3 97 1 0.0 3 0 0 0 1 98 3 0.0 3 0 0 0 3 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 4 0.0 3 0 0 1 3 103 31 0.0 3 0 0 1 30 104 20 0.0 3 0 0 0 20 107 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 112 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 124 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 129 3 0.0 3 0 0 0 3 130 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 140 2 0.0 3 0 0 0 2 146 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 158 2 0.0 3 0 0 0 2 160 1 0.0 3 0 0 0 1 161 3 0.0 3 0 0 0 3 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 3 0.0 3 0 0 0 3 177 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 187 3 0.0 3 0 0 0 3 188 2 0.0 3 0 0 0 2 190 1 0.0 3 0 0 0 1 197 2 0.0 3 0 0 0 2 199 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 204 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (52 us/read; 1.15 M reads/minute). === Summary === Total reads processed: 192 Reads with adapters: 3 (1.6%) Reads written (passing filters): 192 (100.0%) Total basepairs processed: 29,199 bp Total written (filtered): 29,190 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 3 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 33.3% C: 33.3% G: 33.3% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3 3.0 0 3