This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 164.84 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 27,065 (0.7%) Reads that were too short: 9,314 (0.3%) Reads written (passing filters): 17,751 (0.5%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 3,735,252 bp (0.3%) === Adapter 1 === Sequence: GGTTCT; Type: regular 5'; Length: 6; Trimmed: 27065 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1000 887.3 0 1000 7 70 887.3 0 70 8 33 887.3 0 33 9 71 887.3 0 71 10 86 887.3 0 86 11 38 887.3 0 38 12 35 887.3 0 35 13 33 887.3 0 33 14 26 887.3 0 26 15 43 887.3 0 43 16 46 887.3 0 46 17 30 887.3 0 30 18 33 887.3 0 33 19 49 887.3 0 49 20 53 887.3 0 53 21 42 887.3 0 42 22 67 887.3 0 67 23 573 887.3 0 573 24 603 887.3 0 603 25 723 887.3 0 723 26 35 887.3 0 35 27 26 887.3 0 26 28 36 887.3 0 36 29 44 887.3 0 44 30 36 887.3 0 36 31 30 887.3 0 30 32 28 887.3 0 28 33 50 887.3 0 50 34 48 887.3 0 48 35 148 887.3 0 148 36 28 887.3 0 28 37 44 887.3 0 44 38 30 887.3 0 30 39 38 887.3 0 38 40 34 887.3 0 34 41 25 887.3 0 25 42 25 887.3 0 25 43 27 887.3 0 27 44 21 887.3 0 21 45 30 887.3 0 30 46 30 887.3 0 30 47 27 887.3 0 27 48 28 887.3 0 28 49 34 887.3 0 34 50 46 887.3 0 46 51 20 887.3 0 20 52 32 887.3 0 32 53 16 887.3 0 16 54 31 887.3 0 31 55 25 887.3 0 25 56 42 887.3 0 42 57 33 887.3 0 33 58 27 887.3 0 27 59 25 887.3 0 25 60 25 887.3 0 25 61 26 887.3 0 26 62 31 887.3 0 31 63 22 887.3 0 22 64 37 887.3 0 37 65 31 887.3 0 31 66 25 887.3 0 25 67 25 887.3 0 25 68 24 887.3 0 24 69 29 887.3 0 29 70 37 887.3 0 37 71 22 887.3 0 22 72 22 887.3 0 22 73 22 887.3 0 22 74 51 887.3 0 51 75 38 887.3 0 38 76 26 887.3 0 26 77 27 887.3 0 27 78 31 887.3 0 31 79 16 887.3 0 16 80 16 887.3 0 16 81 53 887.3 0 53 82 42 887.3 0 42 83 31 887.3 0 31 84 51 887.3 0 51 85 37 887.3 0 37 86 51 887.3 0 51 87 87 887.3 0 87 88 1521 887.3 0 1521 89 723 887.3 0 723 90 442 887.3 0 442 91 31 887.3 0 31 92 28 887.3 0 28 93 29 887.3 0 29 94 28 887.3 0 28 95 31 887.3 0 31 96 110 887.3 0 110 97 62 887.3 0 62 98 50 887.3 0 50 99 55 887.3 0 55 100 91 887.3 0 91 101 1668 887.3 0 1668 102 698 887.3 0 698 103 793 887.3 0 793 104 68 887.3 0 68 105 38 887.3 0 38 106 49 887.3 0 49 107 18 887.3 0 18 108 16 887.3 0 16 109 27 887.3 0 27 110 32 887.3 0 32 111 30 887.3 0 30 112 31 887.3 0 31 113 50 887.3 0 50 114 39 887.3 0 39 115 22 887.3 0 22 116 25 887.3 0 25 117 36 887.3 0 36 118 61 887.3 0 61 119 26 887.3 0 26 120 38 887.3 0 38 121 34 887.3 0 34 122 29 887.3 0 29 123 31 887.3 0 31 124 41 887.3 0 41 125 44 887.3 0 44 126 96 887.3 0 96 127 760 887.3 0 760 128 549 887.3 0 549 129 425 887.3 0 425 130 38 887.3 0 38 131 31 887.3 0 31 132 54 887.3 0 54 133 33 887.3 0 33 134 51 887.3 0 51 135 35 887.3 0 35 136 44 887.3 0 44 137 18 887.3 0 18 138 18 887.3 0 18 139 29 887.3 0 29 140 31 887.3 0 31 141 44 887.3 0 44 142 176 887.3 0 176 143 27 887.3 0 27 144 33 887.3 0 33 145 85 887.3 0 85 146 53 887.3 0 53 147 53 887.3 0 53 148 148 887.3 0 148 149 158 887.3 0 158 150 31 887.3 0 31 151 35 887.3 0 35 152 28 887.3 0 28 153 19 887.3 0 19 154 28 887.3 0 28 155 35 887.3 0 35 156 34 887.3 0 34 157 23 887.3 0 23 158 31 887.3 0 31 159 27 887.3 0 27 160 18 887.3 0 18 161 37 887.3 0 37 162 31 887.3 0 31 163 23 887.3 0 23 164 19 887.3 0 19 165 20 887.3 0 20 166 11 887.3 0 11 167 34 887.3 0 34 168 20 887.3 0 20 169 35 887.3 0 35 170 27 887.3 0 27 171 27 887.3 0 27 172 27 887.3 0 27 173 15 887.3 0 15 174 60 887.3 0 60 175 28 887.3 0 28 176 45 887.3 0 45 177 44 887.3 0 44 178 38 887.3 0 38 179 23 887.3 0 23 180 23 887.3 0 23 181 30 887.3 0 30 182 31 887.3 0 31 183 25 887.3 0 25 184 24 887.3 0 24 185 25 887.3 0 25 186 32 887.3 0 32 187 31 887.3 0 31 188 71 887.3 0 71 189 29 887.3 0 29 190 40 887.3 0 40 191 21 887.3 0 21 192 29 887.3 0 29 193 39 887.3 0 39 194 37 887.3 0 37 195 58 887.3 0 58 196 46 887.3 0 46 197 30 887.3 0 30 198 37 887.3 0 37 199 29 887.3 0 29 200 98 887.3 0 98 201 171 887.3 0 171 202 134 887.3 0 134 203 48 887.3 0 48 204 39 887.3 0 39 205 42 887.3 0 42 206 33 887.3 0 33 207 66 887.3 0 66 208 65 887.3 0 65 209 62 887.3 0 62 210 42 887.3 0 42 211 40 887.3 0 40 212 27 887.3 0 27 213 50 887.3 0 50 214 49 887.3 0 49 215 58 887.3 0 58 216 70 887.3 0 70 217 58 887.3 0 58 218 90 887.3 0 90 219 42 887.3 0 42 220 24 887.3 0 24 221 30 887.3 0 30 222 44 887.3 0 44 223 30 887.3 0 30 224 33 887.3 0 33 225 39 887.3 0 39 226 40 887.3 0 40 227 31 887.3 0 31 228 38 887.3 0 38 229 31 887.3 0 31 230 39 887.3 0 39 231 56 887.3 0 56 232 44 887.3 0 44 233 46 887.3 0 46 234 114 887.3 0 114 235 102 887.3 0 102 236 84 887.3 0 84 237 94 887.3 0 94 238 66 887.3 0 66 239 124 887.3 0 124 240 109 887.3 0 109 241 126 887.3 0 126 242 76 887.3 0 76 243 45 887.3 0 45 244 39 887.3 0 39 245 18 887.3 0 18 246 50 887.3 0 50 247 37 887.3 0 37 248 46 887.3 0 46 249 43 887.3 0 43 250 36 887.3 0 36 251 32 887.3 0 32 252 16 887.3 0 16 253 35 887.3 0 35 254 59 887.3 0 59 255 90 887.3 0 90 256 78 887.3 0 78 257 64 887.3 0 64 258 57 887.3 0 57 259 540 887.3 0 540 260 825 887.3 0 825 261 696 887.3 0 696 262 224 887.3 0 224 263 96 887.3 0 96 264 34 887.3 0 34 265 27 887.3 0 27 266 52 887.3 0 52 267 45 887.3 0 45 268 52 887.3 0 52 269 26 887.3 0 26 270 29 887.3 0 29 271 28 887.3 0 28 272 28 887.3 0 28 273 35 887.3 0 35 274 31 887.3 0 31 275 37 887.3 0 37 276 61 887.3 0 61 277 44 887.3 0 44 278 72 887.3 0 72 279 698 887.3 0 698 280 699 887.3 0 699 281 669 887.3 0 669 282 42 887.3 0 42 283 60 887.3 0 60 284 43 887.3 0 43 285 21 887.3 0 21 286 39 887.3 0 39 287 28 887.3 0 28 288 42 887.3 0 42 289 30 887.3 0 30 290 28 887.3 0 28 291 63 887.3 0 63 292 83 887.3 0 83 293 114 887.3 0 114 294 61 887.3 0 61 295 93 887.3 0 93 296 63 887.3 0 63 297 102 887.3 0 102 298 47 887.3 0 47 299 25 887.3 0 25 300 50 887.3 0 50 301 52 887.3 0 52 Finished in 1.19 s (67 us/read; 0.90 M reads/minute). === Summary === Total reads processed: 17,751 Reads with adapters: 1,019 (5.7%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 1,011 (5.7%) Total basepairs processed: 3,735,252 bp Total written (filtered): 274,514 bp (7.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1019 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 20 30 0.0 4 30 22 10 0.0 4 1 8 1 23 961 0.0 4 935 25 0 0 1 24 5 0.0 4 1 4 38 2 0.0 4 0 0 0 0 2 39 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 1 107 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 1 148 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.06 s (59 us/read; 1.01 M reads/minute). === Summary === Total reads processed: 1,011 Reads with adapters: 103 (10.2%) Reads written (passing filters): 1,011 (100.0%) Total basepairs processed: 274,514 bp Total written (filtered): 267,374 bp (97.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 103 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.9% C: 18.4% G: 72.8% T: 3.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 9 25 0.0 0 1 24 10 45 0.0 1 2 43 11 9 0.0 1 0 9 257 4 0.0 1 4 263 1 0.0 1 1 267 17 0.0 1 16 1 268 2 0.0 1 2