This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 169.97 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 122,806 (3.4%) Reads that were too short: 15,288 (0.4%) Reads written (passing filters): 107,518 (3.0%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 29,581,193 bp (2.7%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 122806 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 608 887.3 0 608 7 1621 887.3 0 1621 8 49658 887.3 0 49658 9 37495 887.3 0 37495 10 252 887.3 0 252 11 67 887.3 0 67 12 17 887.3 0 17 13 35 887.3 0 35 14 31 887.3 0 31 15 52 887.3 0 52 16 48 887.3 0 48 17 41 887.3 0 41 18 56 887.3 0 56 19 88 887.3 0 88 20 58 887.3 0 58 21 23 887.3 0 23 22 22 887.3 0 22 23 31 887.3 0 31 24 28 887.3 0 28 25 22 887.3 0 22 26 30 887.3 0 30 27 35 887.3 0 35 28 150 887.3 0 150 29 215 887.3 0 215 30 89 887.3 0 89 31 19 887.3 0 19 32 37 887.3 0 37 33 35 887.3 0 35 34 38 887.3 0 38 35 47 887.3 0 47 36 37 887.3 0 37 37 45 887.3 0 45 38 29 887.3 0 29 39 20 887.3 0 20 40 17 887.3 0 17 41 27 887.3 0 27 42 35 887.3 0 35 43 66 887.3 0 66 44 121 887.3 0 121 45 755 887.3 0 755 46 457 887.3 0 457 47 641 887.3 0 641 48 23 887.3 0 23 49 20 887.3 0 20 50 17 887.3 0 17 51 27 887.3 0 27 52 24 887.3 0 24 53 24 887.3 0 24 54 20 887.3 0 20 55 20 887.3 0 20 56 19 887.3 0 19 57 24 887.3 0 24 58 21 887.3 0 21 59 17 887.3 0 17 60 24 887.3 0 24 61 20 887.3 0 20 62 19 887.3 0 19 63 29 887.3 0 29 64 23 887.3 0 23 65 29 887.3 0 29 66 28 887.3 0 28 67 23 887.3 0 23 68 88 887.3 0 88 69 61 887.3 0 61 70 46 887.3 0 46 71 151 887.3 0 151 72 182 887.3 0 182 73 140 887.3 0 140 74 74 887.3 0 74 75 43 887.3 0 43 76 156 887.3 0 156 77 30 887.3 0 30 78 34 887.3 0 34 79 34 887.3 0 34 80 59 887.3 0 59 81 75 887.3 0 75 82 377 887.3 0 377 83 134 887.3 0 134 84 103 887.3 0 103 85 146 887.3 0 146 86 93 887.3 0 93 87 27 887.3 0 27 88 53 887.3 0 53 89 35 887.3 0 35 90 24 887.3 0 24 91 42 887.3 0 42 92 39 887.3 0 39 93 23 887.3 0 23 94 36 887.3 0 36 95 272 887.3 0 272 96 368 887.3 0 368 97 210 887.3 0 210 98 41 887.3 0 41 99 30 887.3 0 30 100 32 887.3 0 32 101 37 887.3 0 37 102 28 887.3 0 28 103 29 887.3 0 29 104 24 887.3 0 24 105 29 887.3 0 29 106 23 887.3 0 23 107 25 887.3 0 25 108 19 887.3 0 19 109 29 887.3 0 29 110 42 887.3 0 42 111 45 887.3 0 45 112 81 887.3 0 81 113 484 887.3 0 484 114 428 887.3 0 428 115 318 887.3 0 318 116 261 887.3 0 261 117 49 887.3 0 49 118 32 887.3 0 32 119 36 887.3 0 36 120 56 887.3 0 56 121 56 887.3 0 56 122 47 887.3 0 47 123 55 887.3 0 55 124 71 887.3 0 71 125 47 887.3 0 47 126 71 887.3 0 71 127 43 887.3 0 43 128 55 887.3 0 55 129 65 887.3 0 65 130 76 887.3 0 76 131 63 887.3 0 63 132 83 887.3 0 83 133 51 887.3 0 51 134 102 887.3 0 102 135 196 887.3 0 196 136 235 887.3 0 235 137 258 887.3 0 258 138 751 887.3 0 751 139 421 887.3 0 421 140 198 887.3 0 198 141 68 887.3 0 68 142 102 887.3 0 102 143 171 887.3 0 171 144 127 887.3 0 127 145 84 887.3 0 84 146 114 887.3 0 114 147 81 887.3 0 81 148 59 887.3 0 59 149 76 887.3 0 76 150 64 887.3 0 64 151 63 887.3 0 63 152 68 887.3 0 68 153 35 887.3 0 35 154 50 887.3 0 50 155 35 887.3 0 35 156 48 887.3 0 48 157 48 887.3 0 48 158 35 887.3 0 35 159 48 887.3 0 48 160 27 887.3 0 27 161 38 887.3 0 38 162 114 887.3 0 114 163 97 887.3 0 97 164 122 887.3 0 122 165 50 887.3 0 50 166 53 887.3 0 53 167 171 887.3 0 171 168 257 887.3 0 257 169 191 887.3 0 191 170 103 887.3 0 103 171 42 887.3 0 42 172 44 887.3 0 44 173 46 887.3 0 46 174 46 887.3 0 46 175 28 887.3 0 28 176 29 887.3 0 29 177 52 887.3 0 52 178 70 887.3 0 70 179 121 887.3 0 121 180 127 887.3 0 127 181 85 887.3 0 85 182 51 887.3 0 51 183 81 887.3 0 81 184 412 887.3 0 412 185 485 887.3 0 485 186 367 887.3 0 367 187 69 887.3 0 69 188 43 887.3 0 43 189 47 887.3 0 47 190 59 887.3 0 59 191 53 887.3 0 53 192 39 887.3 0 39 193 44 887.3 0 44 194 45 887.3 0 45 195 49 887.3 0 49 196 32 887.3 0 32 197 37 887.3 0 37 198 64 887.3 0 64 199 132 887.3 0 132 200 122 887.3 0 122 201 72 887.3 0 72 202 63 887.3 0 63 203 68 887.3 0 68 204 258 887.3 0 258 205 293 887.3 0 293 206 239 887.3 0 239 207 105 887.3 0 105 208 95 887.3 0 95 209 85 887.3 0 85 210 100 887.3 0 100 211 74 887.3 0 74 212 61 887.3 0 61 213 68 887.3 0 68 214 88 887.3 0 88 215 133 887.3 0 133 216 142 887.3 0 142 217 68 887.3 0 68 218 55 887.3 0 55 219 55 887.3 0 55 220 66 887.3 0 66 221 67 887.3 0 67 222 77 887.3 0 77 223 131 887.3 0 131 224 330 887.3 0 330 225 2387 887.3 0 2387 226 3075 887.3 0 3075 227 1703 887.3 0 1703 228 594 887.3 0 594 229 71 887.3 0 71 230 44 887.3 0 44 231 53 887.3 0 53 232 64 887.3 0 64 233 46 887.3 0 46 234 66 887.3 0 66 235 100 887.3 0 100 236 120 887.3 0 120 237 51 887.3 0 51 238 40 887.3 0 40 239 92 887.3 0 92 240 86 887.3 0 86 241 79 887.3 0 79 242 72 887.3 0 72 243 106 887.3 0 106 244 92 887.3 0 92 245 44 887.3 0 44 246 57 887.3 0 57 247 43 887.3 0 43 248 55 887.3 0 55 249 48 887.3 0 48 250 37 887.3 0 37 251 26 887.3 0 26 252 38 887.3 0 38 253 52 887.3 0 52 254 44 887.3 0 44 255 56 887.3 0 56 256 42 887.3 0 42 257 61 887.3 0 61 258 56 887.3 0 56 259 115 887.3 0 115 260 68 887.3 0 68 261 53 887.3 0 53 262 45 887.3 0 45 263 45 887.3 0 45 264 70 887.3 0 70 265 97 887.3 0 97 266 89 887.3 0 89 267 39 887.3 0 39 268 41 887.3 0 41 269 63 887.3 0 63 270 42 887.3 0 42 271 131 887.3 0 131 272 81 887.3 0 81 273 66 887.3 0 66 274 52 887.3 0 52 275 59 887.3 0 59 276 48 887.3 0 48 277 36 887.3 0 36 278 44 887.3 0 44 279 94 887.3 0 94 280 98 887.3 0 98 281 148 887.3 0 148 282 34 887.3 0 34 283 36 887.3 0 36 284 32 887.3 0 32 285 41 887.3 0 41 286 52 887.3 0 52 287 35 887.3 0 35 288 30 887.3 0 30 289 58 887.3 0 58 290 51 887.3 0 51 291 51 887.3 0 51 292 47 887.3 0 47 293 59 887.3 0 59 294 58 887.3 0 58 295 67 887.3 0 67 296 75 887.3 0 75 297 82 887.3 0 82 298 152 887.3 0 152 299 87 887.3 0 87 300 60 887.3 0 60 301 236 887.3 0 236 Finished in 7.92 s (74 us/read; 0.81 M reads/minute). === Summary === Total reads processed: 107,518 Reads with adapters: 48,559 (45.2%) Reads that were too short: 87 (0.1%) Reads written (passing filters): 48,472 (45.1%) Total basepairs processed: 29,581,193 bp Total written (filtered): 13,355,176 bp (45.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 48559 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 18 0.0 3 0 0 14 4 16 372 0.0 3 0 328 30 14 17 47634 0.0 3 45396 1862 301 75 18 133 0.0 3 15 98 14 6 19 101 0.0 3 5 2 67 27 20 50 0.0 3 5 0 0 45 23 1 0.0 3 1 24 9 0.0 3 0 0 0 9 27 4 0.0 3 0 0 0 4 32 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 35 5 0.0 3 0 0 0 5 36 2 0.0 3 0 0 0 2 38 3 0.0 3 0 0 0 3 43 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 0 2 52 4 0.0 3 0 0 0 4 53 2 0.0 3 0 0 0 2 54 20 0.0 3 0 0 0 20 55 1 0.0 3 0 0 0 1 56 14 0.0 3 0 0 0 14 57 2 0.0 3 0 0 0 2 58 9 0.0 3 0 0 0 9 61 4 0.0 3 0 0 0 4 65 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 0 2 71 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 12 0.0 3 0 0 0 12 79 1 0.0 3 0 0 0 1 81 5 0.0 3 0 0 0 5 82 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 104 3 0.0 3 0 0 0 3 109 3 0.0 3 0 0 0 3 113 1 0.0 3 0 0 0 1 114 2 0.0 3 0 0 0 2 117 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 126 13 0.0 3 0 0 1 12 127 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 0 2 129 5 0.0 3 0 0 2 3 133 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 145 4 0.0 3 0 0 0 4 146 3 0.0 3 0 0 0 3 147 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 3 0.0 3 0 0 0 3 151 2 0.0 3 0 0 1 1 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 160 2 0.0 3 0 0 0 2 161 1 0.0 3 0 0 0 1 163 8 0.0 3 0 0 0 8 164 4 0.0 3 0 0 0 4 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 168 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 2 0.0 3 0 0 0 2 172 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 179 4 0.0 3 0 0 0 4 180 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 0 2 194 1 0.0 3 0 0 0 1 197 2 0.0 3 0 0 0 2 199 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 217 2 0.0 3 0 0 0 2 218 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 221 5 0.0 3 0 0 0 5 229 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 247 2 0.0 3 0 0 0 2 250 3 0.0 3 0 0 0 3 256 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.83 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 48,472 Reads with adapters: 502 (1.0%) Reads written (passing filters): 48,472 (100.0%) Total basepairs processed: 13,355,176 bp Total written (filtered): 13,353,375 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 502 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 29.7% C: 7.8% G: 59.4% T: 3.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 222 757.4 0 222 4 266 189.3 0 266 5 13 47.3 0 13 6 1 11.8 0 1