This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 182.89 s (50 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 1,000,140 (27.5%) Reads that were too short: 17,243 (0.5%) Reads written (passing filters): 982,897 (27.0%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 185,617,186 bp (17.0%) === Adapter 1 === Sequence: AGAAGA; Type: regular 5'; Length: 6; Trimmed: 1000140 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2112 887.3 0 2112 7 103360 887.3 0 103360 8 81532 887.3 0 81532 9 206 887.3 0 206 10 149 887.3 0 149 11 172 887.3 0 172 12 129 887.3 0 129 13 71 887.3 0 71 14 72 887.3 0 72 15 98 887.3 0 98 16 69 887.3 0 69 17 84 887.3 0 84 18 91 887.3 0 91 19 73 887.3 0 73 20 82 887.3 0 82 21 79 887.3 0 79 22 71 887.3 0 71 23 82 887.3 0 82 24 119 887.3 0 119 25 169 887.3 0 169 26 242 887.3 0 242 27 345 887.3 0 345 28 113 887.3 0 113 29 114 887.3 0 114 30 116 887.3 0 116 31 85 887.3 0 85 32 87 887.3 0 87 33 71 887.3 0 71 34 102 887.3 0 102 35 119 887.3 0 119 36 109 887.3 0 109 37 152 887.3 0 152 38 76 887.3 0 76 39 68 887.3 0 68 40 80 887.3 0 80 41 70 887.3 0 70 42 91 887.3 0 91 43 137 887.3 0 137 44 95 887.3 0 95 45 99 887.3 0 99 46 101 887.3 0 101 47 79 887.3 0 79 48 101 887.3 0 101 49 105 887.3 0 105 50 110 887.3 0 110 51 105 887.3 0 105 52 121 887.3 0 121 53 131 887.3 0 131 54 137 887.3 0 137 55 110 887.3 0 110 56 124 887.3 0 124 57 194 887.3 0 194 58 201 887.3 0 201 59 112 887.3 0 112 60 186 887.3 0 186 61 177 887.3 0 177 62 191 887.3 0 191 63 162 887.3 0 162 64 154 887.3 0 154 65 121 887.3 0 121 66 130 887.3 0 130 67 149 887.3 0 149 68 205 887.3 0 205 69 246 887.3 0 246 70 140 887.3 0 140 71 134 887.3 0 134 72 518 887.3 0 518 73 257 887.3 0 257 74 1004 887.3 0 1004 75 335 887.3 0 335 76 1491 887.3 0 1491 77 467 887.3 0 467 78 352 887.3 0 352 79 1821 887.3 0 1821 80 861 887.3 0 861 81 2186 887.3 0 2186 82 1033 887.3 0 1033 83 1284 887.3 0 1284 84 406 887.3 0 406 85 544 887.3 0 544 86 457 887.3 0 457 87 144 887.3 0 144 88 139 887.3 0 139 89 215 887.3 0 215 90 129 887.3 0 129 91 133 887.3 0 133 92 326 887.3 0 326 93 257 887.3 0 257 94 416 887.3 0 416 95 401 887.3 0 401 96 263 887.3 0 263 97 218 887.3 0 218 98 320 887.3 0 320 99 279 887.3 0 279 100 249 887.3 0 249 101 465 887.3 0 465 102 392 887.3 0 392 103 361 887.3 0 361 104 461 887.3 0 461 105 1074 887.3 0 1074 106 362 887.3 0 362 107 445 887.3 0 445 108 698 887.3 0 698 109 467 887.3 0 467 110 547 887.3 0 547 111 806 887.3 0 806 112 803 887.3 0 803 113 1221 887.3 0 1221 114 1031 887.3 0 1031 115 1698 887.3 0 1698 116 2131 887.3 0 2131 117 4518 887.3 0 4518 118 54314 887.3 0 54314 119 61737 887.3 0 61737 120 78738 887.3 0 78738 121 4014 887.3 0 4014 122 4314 887.3 0 4314 123 3710 887.3 0 3710 124 602 887.3 0 602 125 444 887.3 0 444 126 431 887.3 0 431 127 582 887.3 0 582 128 513 887.3 0 513 129 530 887.3 0 530 130 704 887.3 0 704 131 1117 887.3 0 1117 132 2353 887.3 0 2353 133 914 887.3 0 914 134 1112 887.3 0 1112 135 1731 887.3 0 1731 136 1210 887.3 0 1210 137 1694 887.3 0 1694 138 2519 887.3 0 2519 139 8701 887.3 0 8701 140 24455 887.3 0 24455 141 26830 887.3 0 26830 142 14256 887.3 0 14256 143 4707 887.3 0 4707 144 10755 887.3 0 10755 145 129932 887.3 0 129932 146 125580 887.3 0 125580 147 108611 887.3 0 108611 148 6663 887.3 0 6663 149 3637 887.3 0 3637 150 1021 887.3 0 1021 151 527 887.3 0 527 152 715 887.3 0 715 153 833 887.3 0 833 154 703 887.3 0 703 155 1052 887.3 0 1052 156 711 887.3 0 711 157 2328 887.3 0 2328 158 2115 887.3 0 2115 159 2178 887.3 0 2178 160 2965 887.3 0 2965 161 1647 887.3 0 1647 162 754 887.3 0 754 163 1187 887.3 0 1187 164 2943 887.3 0 2943 165 15635 887.3 0 15635 166 16394 887.3 0 16394 167 6248 887.3 0 6248 168 621 887.3 0 621 169 288 887.3 0 288 170 228 887.3 0 228 171 155 887.3 0 155 172 214 887.3 0 214 173 164 887.3 0 164 174 209 887.3 0 209 175 179 887.3 0 179 176 133 887.3 0 133 177 146 887.3 0 146 178 118 887.3 0 118 179 128 887.3 0 128 180 126 887.3 0 126 181 167 887.3 0 167 182 177 887.3 0 177 183 194 887.3 0 194 184 187 887.3 0 187 185 152 887.3 0 152 186 186 887.3 0 186 187 180 887.3 0 180 188 168 887.3 0 168 189 176 887.3 0 176 190 155 887.3 0 155 191 162 887.3 0 162 192 227 887.3 0 227 193 260 887.3 0 260 194 363 887.3 0 363 195 215 887.3 0 215 196 130 887.3 0 130 197 157 887.3 0 157 198 140 887.3 0 140 199 188 887.3 0 188 200 189 887.3 0 189 201 147 887.3 0 147 202 140 887.3 0 140 203 106 887.3 0 106 204 144 887.3 0 144 205 158 887.3 0 158 206 168 887.3 0 168 207 191 887.3 0 191 208 150 887.3 0 150 209 218 887.3 0 218 210 178 887.3 0 178 211 166 887.3 0 166 212 109 887.3 0 109 213 111 887.3 0 111 214 128 887.3 0 128 215 145 887.3 0 145 216 292 887.3 0 292 217 450 887.3 0 450 218 641 887.3 0 641 219 424 887.3 0 424 220 188 887.3 0 188 221 177 887.3 0 177 222 122 887.3 0 122 223 149 887.3 0 149 224 126 887.3 0 126 225 187 887.3 0 187 226 342 887.3 0 342 227 280 887.3 0 280 228 197 887.3 0 197 229 126 887.3 0 126 230 154 887.3 0 154 231 134 887.3 0 134 232 120 887.3 0 120 233 123 887.3 0 123 234 117 887.3 0 117 235 113 887.3 0 113 236 114 887.3 0 114 237 188 887.3 0 188 238 137 887.3 0 137 239 146 887.3 0 146 240 140 887.3 0 140 241 139 887.3 0 139 242 99 887.3 0 99 243 149 887.3 0 149 244 149 887.3 0 149 245 123 887.3 0 123 246 182 887.3 0 182 247 191 887.3 0 191 248 152 887.3 0 152 249 125 887.3 0 125 250 111 887.3 0 111 251 123 887.3 0 123 252 120 887.3 0 120 253 121 887.3 0 121 254 120 887.3 0 120 255 122 887.3 0 122 256 124 887.3 0 124 257 126 887.3 0 126 258 117 887.3 0 117 259 121 887.3 0 121 260 178 887.3 0 178 261 153 887.3 0 153 262 198 887.3 0 198 263 169 887.3 0 169 264 218 887.3 0 218 265 224 887.3 0 224 266 148 887.3 0 148 267 152 887.3 0 152 268 146 887.3 0 146 269 101 887.3 0 101 270 124 887.3 0 124 271 115 887.3 0 115 272 125 887.3 0 125 273 118 887.3 0 118 274 115 887.3 0 115 275 136 887.3 0 136 276 132 887.3 0 132 277 156 887.3 0 156 278 169 887.3 0 169 279 147 887.3 0 147 280 118 887.3 0 118 281 156 887.3 0 156 282 123 887.3 0 123 283 129 887.3 0 129 284 129 887.3 0 129 285 120 887.3 0 120 286 117 887.3 0 117 287 131 887.3 0 131 288 159 887.3 0 159 289 151 887.3 0 151 290 137 887.3 0 137 291 154 887.3 0 154 292 163 887.3 0 163 293 145 887.3 0 145 294 149 887.3 0 149 295 110 887.3 0 110 296 159 887.3 0 159 297 191 887.3 0 191 298 190 887.3 0 190 299 202 887.3 0 202 300 311 887.3 0 311 301 1162 887.3 0 1162 Finished in 55.72 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 982,897 Reads with adapters: 116,186 (11.8%) Reads that were too short: 1,734 (0.2%) Reads written (passing filters): 114,452 (11.6%) Total basepairs processed: 185,617,186 bp Total written (filtered): 29,893,351 bp (16.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 116186 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 10 0.0 2 0 0 0 10 15 33 0.0 3 0 0 20 13 16 645 0.0 3 0 546 63 36 17 99042 0.0 3 94352 3891 634 165 18 230 0.0 3 18 183 10 19 19 2708 0.0 3 2348 126 150 84 20 107 0.0 3 1 8 1 97 21 4 0.0 3 0 1 1 2 22 8 0.0 3 1 0 0 7 23 45 0.0 3 0 0 0 45 24 1 0.0 3 0 0 1 25 11 0.0 3 2 0 0 9 26 6540 0.0 3 1 0 66 6473 27 45 0.0 3 0 0 0 45 28 16 0.0 3 0 0 1 15 29 760 0.0 3 0 0 26 734 30 7 0.0 3 0 0 0 7 31 2 0.0 3 1 0 0 1 32 7 0.0 3 0 0 0 7 33 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 35 2 0.0 3 0 0 0 2 37 2 0.0 3 0 0 0 2 39 1 0.0 3 1 40 2 0.0 3 0 0 0 2 41 2 0.0 3 0 0 0 2 42 6 0.0 3 0 0 0 6 43 1 0.0 3 0 0 0 1 45 6 0.0 3 0 0 0 6 46 2 0.0 3 0 0 0 2 47 17 0.0 3 0 0 0 17 48 15 0.0 3 0 0 0 15 49 13 0.0 3 0 0 0 13 50 281 0.0 3 0 0 3 278 51 7 0.0 3 0 0 0 7 52 136 0.0 3 0 0 2 134 53 150 0.0 3 0 0 2 148 54 2 0.0 3 0 0 0 2 56 4 0.0 3 0 0 1 3 58 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 60 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 66 2 0.0 3 0 0 0 2 68 3 0.0 3 0 0 0 3 69 2 0.0 3 0 0 0 2 70 8 0.0 3 0 0 1 7 71 2 0.0 3 0 0 0 2 72 861 0.0 3 0 0 8 853 73 475 0.0 3 0 0 0 475 74 1985 0.0 3 0 0 6 1979 75 2 0.0 3 0 0 0 2 76 57 0.0 3 0 0 2 55 77 229 0.0 3 0 0 4 225 78 9 0.0 3 0 0 0 9 79 25 0.0 3 0 0 0 25 80 4 0.0 3 0 0 0 4 82 2 0.0 3 0 0 0 2 83 3 0.0 3 0 0 0 3 84 2 0.0 3 0 0 0 2 85 3 0.0 3 0 0 0 3 86 27 0.0 3 0 0 1 26 87 15 0.0 3 0 0 0 15 88 3 0.0 3 0 0 1 2 89 5 0.0 3 0 0 0 5 90 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 95 3 0.0 3 0 0 0 3 96 5 0.0 3 0 0 0 5 97 7 0.0 3 0 0 0 7 98 7 0.0 3 0 0 1 6 99 9 0.0 3 0 0 0 9 100 11 0.0 3 0 0 0 11 101 4 0.0 3 0 0 0 4 102 2 0.0 3 0 0 0 2 103 3 0.0 3 0 0 0 3 104 3 0.0 3 0 0 0 3 106 5 0.0 3 0 0 0 5 107 4 0.0 3 0 0 0 4 108 8 0.0 3 0 0 0 8 109 4 0.0 3 0 0 0 4 110 7 0.0 3 0 0 0 7 111 25 0.0 3 0 0 1 24 112 4 0.0 3 0 0 0 4 113 3 0.0 3 0 0 1 2 114 4 0.0 3 0 0 1 3 116 5 0.0 3 0 0 0 5 117 3 0.0 3 0 0 0 3 118 2 0.0 3 0 0 0 2 119 5 0.0 3 0 0 0 5 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 124 14 0.0 3 0 0 1 13 125 21 0.0 3 0 0 0 21 126 18 0.0 3 0 0 0 18 127 35 0.0 3 0 0 1 34 128 82 0.0 3 0 0 6 76 129 27 0.0 3 0 0 1 26 130 3 0.0 3 0 0 0 3 132 2 0.0 3 0 0 0 2 133 86 0.0 3 0 0 0 86 134 11 0.0 3 0 0 0 11 135 6 0.0 3 0 0 0 6 136 5 0.0 3 0 0 0 5 137 11 0.0 3 0 0 0 11 138 187 0.0 3 0 0 3 184 139 40 0.0 3 0 0 0 40 140 32 0.0 3 0 0 0 32 141 112 0.0 3 0 0 3 109 142 10 0.0 3 0 0 0 10 143 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 145 3 0.0 3 0 0 0 3 146 72 0.0 3 0 0 0 72 147 59 0.0 3 0 0 4 55 148 23 0.0 3 0 0 2 21 149 1 0.0 3 0 0 0 1 150 9 0.0 3 0 0 0 9 151 22 0.0 3 0 0 0 22 152 7 0.0 3 0 0 1 6 153 12 0.0 3 0 0 0 12 154 2 0.0 3 0 0 0 2 155 7 0.0 3 0 0 0 7 156 207 0.0 3 0 0 9 198 157 4 0.0 3 0 0 0 4 158 11 0.0 3 0 0 0 11 159 2 0.0 3 0 0 0 2 160 4 0.0 3 0 0 0 4 161 4 0.0 3 0 0 1 3 162 7 0.0 3 0 0 0 7 163 5 0.0 3 0 0 0 5 164 3 0.0 3 0 0 0 3 165 10 0.0 3 0 0 1 9 166 38 0.0 3 0 0 1 37 167 3 0.0 3 0 0 1 2 169 1 0.0 3 0 0 0 1 170 11 0.0 3 0 0 0 11 171 3 0.0 3 0 0 0 3 172 4 0.0 3 0 0 0 4 173 2 0.0 3 0 0 0 2 174 15 0.0 3 0 0 0 15 175 10 0.0 3 0 0 0 10 176 8 0.0 3 0 0 0 8 177 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 180 8 0.0 3 0 0 0 8 182 3 0.0 3 0 0 0 3 183 7 0.0 3 0 0 0 7 184 2 0.0 3 0 0 1 1 185 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 190 4 0.0 3 0 0 0 4 191 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 196 11 0.0 3 0 0 0 11 197 1 0.0 3 0 0 0 1 198 2 0.0 3 0 0 0 2 199 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 5 0.0 3 0 0 0 5 208 1 0.0 3 0 0 0 1 209 2 0.0 3 0 1 0 1 211 2 0.0 3 0 0 0 2 216 2 0.0 3 0 0 0 2 217 20 0.0 3 0 0 0 20 218 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 221 20 0.0 3 0 0 0 20 222 3 0.0 3 0 0 0 3 225 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 238 3 0.0 3 0 0 0 3 242 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 254 2 0.0 3 0 0 0 2 267 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 6 0.0 3 0 0 0 6 280 7 0.0 3 0 0 1 6 281 10 0.0 3 0 0 0 10 282 7 0.0 3 0 0 1 6 283 2 0.0 3 0 0 0 2 285 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 6.48 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 114,452 Reads with adapters: 1,524 (1.3%) Reads written (passing filters): 114,452 (100.0%) Total basepairs processed: 29,893,351 bp Total written (filtered): 29,888,708 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1524 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 11.7% C: 41.1% G: 44.5% T: 2.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1475 1788.3 0 1475 4 40 447.1 0 40 5 3 111.8 0 3 6 5 27.9 0 5 13 1 0.0 1 0 1