This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGACG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 172.93 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 236,195 (6.5%) Reads that were too short: 10,753 (0.3%) Reads written (passing filters): 225,442 (6.2%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 62,987,088 bp (5.8%) === Adapter 1 === Sequence: TCGACG; Type: regular 5'; Length: 6; Trimmed: 236195 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1207 887.3 0 1207 7 4777 887.3 0 4777 8 103936 887.3 0 103936 9 89047 887.3 0 89047 10 174 887.3 0 174 11 42 887.3 0 42 12 32 887.3 0 32 13 37 887.3 0 37 14 19 887.3 0 19 15 36 887.3 0 36 16 13 887.3 0 13 17 18 887.3 0 18 18 13 887.3 0 13 19 24 887.3 0 24 20 14 887.3 0 14 21 18 887.3 0 18 22 20 887.3 0 20 23 8 887.3 0 8 24 7 887.3 0 7 25 13 887.3 0 13 26 17 887.3 0 17 27 18 887.3 0 18 28 18 887.3 0 18 29 9 887.3 0 9 30 23 887.3 0 23 31 14 887.3 0 14 32 14 887.3 0 14 33 14 887.3 0 14 34 31 887.3 0 31 35 20 887.3 0 20 36 16 887.3 0 16 37 17 887.3 0 17 38 19 887.3 0 19 39 16 887.3 0 16 40 43 887.3 0 43 41 22 887.3 0 22 42 23 887.3 0 23 43 16 887.3 0 16 44 21 887.3 0 21 45 28 887.3 0 28 46 32 887.3 0 32 47 66 887.3 0 66 48 28 887.3 0 28 49 117 887.3 0 117 50 39 887.3 0 39 51 14 887.3 0 14 52 17 887.3 0 17 53 27 887.3 0 27 54 27 887.3 0 27 55 23 887.3 0 23 56 17 887.3 0 17 57 18 887.3 0 18 58 31 887.3 0 31 59 36 887.3 0 36 60 59 887.3 0 59 61 37 887.3 0 37 62 39 887.3 0 39 63 20 887.3 0 20 64 19 887.3 0 19 65 16 887.3 0 16 66 389 887.3 0 389 67 248 887.3 0 248 68 255 887.3 0 255 69 8 887.3 0 8 70 30 887.3 0 30 71 16 887.3 0 16 72 14 887.3 0 14 73 21 887.3 0 21 74 19 887.3 0 19 75 14 887.3 0 14 76 17 887.3 0 17 77 28 887.3 0 28 78 14 887.3 0 14 79 18 887.3 0 18 80 22 887.3 0 22 81 21 887.3 0 21 82 44 887.3 0 44 83 40 887.3 0 40 84 93 887.3 0 93 85 122 887.3 0 122 86 18 887.3 0 18 87 15 887.3 0 15 88 59 887.3 0 59 89 13 887.3 0 13 90 36 887.3 0 36 91 374 887.3 0 374 92 86 887.3 0 86 93 112 887.3 0 112 94 67 887.3 0 67 95 37 887.3 0 37 96 24 887.3 0 24 97 15 887.3 0 15 98 28 887.3 0 28 99 23 887.3 0 23 100 14 887.3 0 14 101 22 887.3 0 22 102 23 887.3 0 23 103 37 887.3 0 37 104 56 887.3 0 56 105 41 887.3 0 41 106 51 887.3 0 51 107 67 887.3 0 67 108 52 887.3 0 52 109 58 887.3 0 58 110 96 887.3 0 96 111 787 887.3 0 787 112 441 887.3 0 441 113 766 887.3 0 766 114 167 887.3 0 167 115 456 887.3 0 456 116 820 887.3 0 820 117 6645 887.3 0 6645 118 1322 887.3 0 1322 119 2127 887.3 0 2127 120 41 887.3 0 41 121 38 887.3 0 38 122 22 887.3 0 22 123 26 887.3 0 26 124 44 887.3 0 44 125 62 887.3 0 62 126 638 887.3 0 638 127 166 887.3 0 166 128 175 887.3 0 175 129 43 887.3 0 43 130 58 887.3 0 58 131 71 887.3 0 71 132 105 887.3 0 105 133 64 887.3 0 64 134 33 887.3 0 33 135 37 887.3 0 37 136 46 887.3 0 46 137 35 887.3 0 35 138 99 887.3 0 99 139 41 887.3 0 41 140 35 887.3 0 35 141 41 887.3 0 41 142 46 887.3 0 46 143 30 887.3 0 30 144 56 887.3 0 56 145 59 887.3 0 59 146 111 887.3 0 111 147 95 887.3 0 95 148 119 887.3 0 119 149 79 887.3 0 79 150 127 887.3 0 127 151 1098 887.3 0 1098 152 1002 887.3 0 1002 153 680 887.3 0 680 154 237 887.3 0 237 155 29 887.3 0 29 156 42 887.3 0 42 157 42 887.3 0 42 158 28 887.3 0 28 159 47 887.3 0 47 160 47 887.3 0 47 161 37 887.3 0 37 162 184 887.3 0 184 163 106 887.3 0 106 164 117 887.3 0 117 165 35 887.3 0 35 166 44 887.3 0 44 167 27 887.3 0 27 168 32 887.3 0 32 169 51 887.3 0 51 170 33 887.3 0 33 171 28 887.3 0 28 172 33 887.3 0 33 173 56 887.3 0 56 174 32 887.3 0 32 175 22 887.3 0 22 176 35 887.3 0 35 177 33 887.3 0 33 178 34 887.3 0 34 179 165 887.3 0 165 180 149 887.3 0 149 181 92 887.3 0 92 182 75 887.3 0 75 183 171 887.3 0 171 184 248 887.3 0 248 185 70 887.3 0 70 186 24 887.3 0 24 187 45 887.3 0 45 188 40 887.3 0 40 189 60 887.3 0 60 190 46 887.3 0 46 191 85 887.3 0 85 192 69 887.3 0 69 193 49 887.3 0 49 194 25 887.3 0 25 195 30 887.3 0 30 196 29 887.3 0 29 197 42 887.3 0 42 198 48 887.3 0 48 199 51 887.3 0 51 200 41 887.3 0 41 201 31 887.3 0 31 202 27 887.3 0 27 203 45 887.3 0 45 204 45 887.3 0 45 205 50 887.3 0 50 206 47 887.3 0 47 207 29 887.3 0 29 208 48 887.3 0 48 209 23 887.3 0 23 210 65 887.3 0 65 211 91 887.3 0 91 212 144 887.3 0 144 213 78 887.3 0 78 214 66 887.3 0 66 215 59 887.3 0 59 216 69 887.3 0 69 217 157 887.3 0 157 218 303 887.3 0 303 219 361 887.3 0 361 220 308 887.3 0 308 221 67 887.3 0 67 222 27 887.3 0 27 223 57 887.3 0 57 224 120 887.3 0 120 225 87 887.3 0 87 226 98 887.3 0 98 227 77 887.3 0 77 228 106 887.3 0 106 229 103 887.3 0 103 230 59 887.3 0 59 231 304 887.3 0 304 232 299 887.3 0 299 233 373 887.3 0 373 234 218 887.3 0 218 235 154 887.3 0 154 236 112 887.3 0 112 237 110 887.3 0 110 238 70 887.3 0 70 239 81 887.3 0 81 240 63 887.3 0 63 241 45 887.3 0 45 242 86 887.3 0 86 243 91 887.3 0 91 244 117 887.3 0 117 245 192 887.3 0 192 246 210 887.3 0 210 247 246 887.3 0 246 248 211 887.3 0 211 249 155 887.3 0 155 250 85 887.3 0 85 251 93 887.3 0 93 252 114 887.3 0 114 253 93 887.3 0 93 254 139 887.3 0 139 255 110 887.3 0 110 256 61 887.3 0 61 257 72 887.3 0 72 258 51 887.3 0 51 259 59 887.3 0 59 260 40 887.3 0 40 261 47 887.3 0 47 262 35 887.3 0 35 263 39 887.3 0 39 264 66 887.3 0 66 265 84 887.3 0 84 266 74 887.3 0 74 267 76 887.3 0 76 268 67 887.3 0 67 269 40 887.3 0 40 270 58 887.3 0 58 271 98 887.3 0 98 272 109 887.3 0 109 273 101 887.3 0 101 274 60 887.3 0 60 275 40 887.3 0 40 276 51 887.3 0 51 277 44 887.3 0 44 278 96 887.3 0 96 279 133 887.3 0 133 280 161 887.3 0 161 281 81 887.3 0 81 282 150 887.3 0 150 283 209 887.3 0 209 284 171 887.3 0 171 285 98 887.3 0 98 286 104 887.3 0 104 287 125 887.3 0 125 288 88 887.3 0 88 289 73 887.3 0 73 290 65 887.3 0 65 291 122 887.3 0 122 292 113 887.3 0 113 293 200 887.3 0 200 294 118 887.3 0 118 295 79 887.3 0 79 296 80 887.3 0 80 297 52 887.3 0 52 298 89 887.3 0 89 299 128 887.3 0 128 300 130 887.3 0 130 301 129 887.3 0 129 Finished in 16.57 s (74 us/read; 0.82 M reads/minute). === Summary === Total reads processed: 225,442 Reads with adapters: 105,286 (46.7%) Reads that were too short: 175 (0.1%) Reads written (passing filters): 105,111 (46.6%) Total basepairs processed: 62,987,088 bp Total written (filtered): 28,970,778 bp (46.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 105286 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 16 0.0 2 0 0 0 16 15 72 0.0 3 0 0 49 23 16 860 0.0 3 0 761 68 31 17 103267 0.0 3 98218 4252 642 155 18 271 0.0 3 37 197 21 16 19 212 0.0 3 4 0 129 79 20 109 0.0 3 12 2 3 92 22 2 0.0 3 1 0 1 24 1 0.0 3 0 0 0 1 26 1 0.0 3 1 27 3 0.0 3 0 0 0 3 30 4 0.0 3 0 0 0 4 32 1 0.0 3 1 33 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 40 33 0.0 3 0 0 1 32 43 8 0.0 3 0 0 1 7 46 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 53 1 0.0 3 0 0 0 1 54 133 0.0 3 0 0 2 131 55 2 0.0 3 0 0 0 2 56 10 0.0 3 0 0 0 10 57 25 0.0 3 0 0 0 25 58 4 0.0 3 0 0 0 4 59 1 0.0 3 0 0 0 1 60 3 0.0 3 0 0 0 3 61 1 0.0 3 0 0 0 1 63 3 0.0 3 0 0 0 3 64 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 66 6 0.0 3 0 0 0 6 67 4 0.0 3 0 0 0 4 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 80 6 0.0 3 0 0 0 6 81 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 91 5 0.0 3 0 0 1 4 98 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 100 4 0.0 3 0 0 0 4 102 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 104 2 0.0 3 0 0 0 2 105 17 0.0 3 0 0 1 16 106 3 0.0 3 0 0 0 3 107 7 0.0 3 0 0 0 7 108 4 0.0 3 0 0 0 4 109 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 3 0.0 3 0 0 0 3 119 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 3 0.0 3 0 0 0 3 132 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 2 0.0 3 0 0 0 2 141 1 0.0 3 0 0 0 1 142 4 0.0 3 0 0 0 4 144 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 150 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 158 2 0.0 3 0 0 0 2 162 3 0.0 3 0 0 0 3 163 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 23 0.0 3 0 0 0 23 167 2 0.0 3 0 0 0 2 168 1 0.0 3 0 0 0 1 169 22 0.0 3 0 0 1 21 170 3 0.0 3 0 0 0 3 171 1 0.0 3 0 0 0 1 173 2 0.0 3 0 0 0 2 176 2 0.0 3 0 0 0 2 178 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 183 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 185 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 187 3 0.0 3 0 0 0 3 188 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 194 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 0 1 208 2 0.0 3 0 0 0 2 218 1 0.0 3 0 0 0 1 221 6 0.0 3 0 0 0 6 222 1 0.0 3 0 0 0 1 225 2 0.0 3 0 0 0 2 229 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 271 2 0.0 3 0 0 0 2 279 4 0.0 3 0 0 0 4 280 3 0.0 3 0 0 0 3 281 9 0.0 3 0 0 0 9 282 9 0.0 3 0 0 0 9 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 6.11 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 105,111 Reads with adapters: 481 (0.5%) Reads written (passing filters): 105,111 (100.0%) Total basepairs processed: 28,970,778 bp Total written (filtered): 28,969,168 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 481 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 39.5% C: 10.2% G: 44.7% T: 5.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 325 1642.4 0 325 4 146 410.6 0 146 5 9 102.6 0 9 6 1 25.7 0 1