This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCGCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 178.72 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 695,183 (19.1%) Reads that were too short: 51,664 (1.4%) Reads written (passing filters): 643,519 (17.7%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 161,295,636 bp (14.7%) === Adapter 1 === Sequence: GGCGCA; Type: regular 5'; Length: 6; Trimmed: 695183 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1789 887.3 0 1789 7 65121 887.3 0 65121 8 51743 887.3 0 51743 9 293 887.3 0 293 10 256 887.3 0 256 11 201 887.3 0 201 12 238 887.3 0 238 13 398 887.3 0 398 14 485 887.3 0 485 15 519 887.3 0 519 16 453 887.3 0 453 17 902 887.3 0 902 18 1086 887.3 0 1086 19 2262 887.3 0 2262 20 44426 887.3 0 44426 21 37683 887.3 0 37683 22 33169 887.3 0 33169 23 451 887.3 0 451 24 287 887.3 0 287 25 543 887.3 0 543 26 189 887.3 0 189 27 149 887.3 0 149 28 113 887.3 0 113 29 154 887.3 0 154 30 115 887.3 0 115 31 152 887.3 0 152 32 233 887.3 0 233 33 297 887.3 0 297 34 292 887.3 0 292 35 632 887.3 0 632 36 1575 887.3 0 1575 37 398 887.3 0 398 38 533 887.3 0 533 39 998 887.3 0 998 40 549 887.3 0 549 41 613 887.3 0 613 42 1018 887.3 0 1018 43 1116 887.3 0 1116 44 1412 887.3 0 1412 45 1262 887.3 0 1262 46 2199 887.3 0 2199 47 2761 887.3 0 2761 48 6480 887.3 0 6480 49 111497 887.3 0 111497 50 117221 887.3 0 117221 51 76304 887.3 0 76304 52 378 887.3 0 378 53 63 887.3 0 63 54 69 887.3 0 69 55 49 887.3 0 49 56 85 887.3 0 85 57 82 887.3 0 82 58 92 887.3 0 92 59 70 887.3 0 70 60 113 887.3 0 113 61 99 887.3 0 99 62 133 887.3 0 133 63 799 887.3 0 799 64 1085 887.3 0 1085 65 1104 887.3 0 1104 66 66 887.3 0 66 67 51 887.3 0 51 68 63 887.3 0 63 69 24 887.3 0 24 70 53 887.3 0 53 71 60 887.3 0 60 72 47 887.3 0 47 73 82 887.3 0 82 74 77 887.3 0 77 75 66 887.3 0 66 76 47 887.3 0 47 77 48 887.3 0 48 78 37 887.3 0 37 79 47 887.3 0 47 80 48 887.3 0 48 81 59 887.3 0 59 82 37 887.3 0 37 83 61 887.3 0 61 84 59 887.3 0 59 85 57 887.3 0 57 86 77 887.3 0 77 87 60 887.3 0 60 88 73 887.3 0 73 89 59 887.3 0 59 90 259 887.3 0 259 91 58 887.3 0 58 92 69 887.3 0 69 93 174 887.3 0 174 94 63 887.3 0 63 95 65 887.3 0 65 96 82 887.3 0 82 97 51 887.3 0 51 98 74 887.3 0 74 99 53 887.3 0 53 100 46 887.3 0 46 101 56 887.3 0 56 102 41 887.3 0 41 103 49 887.3 0 49 104 47 887.3 0 47 105 49 887.3 0 49 106 82 887.3 0 82 107 44 887.3 0 44 108 37 887.3 0 37 109 46 887.3 0 46 110 58 887.3 0 58 111 61 887.3 0 61 112 72 887.3 0 72 113 53 887.3 0 53 114 52 887.3 0 52 115 58 887.3 0 58 116 105 887.3 0 105 117 226 887.3 0 226 118 580 887.3 0 580 119 606 887.3 0 606 120 126 887.3 0 126 121 74 887.3 0 74 122 65 887.3 0 65 123 73 887.3 0 73 124 113 887.3 0 113 125 109 887.3 0 109 126 231 887.3 0 231 127 93 887.3 0 93 128 60 887.3 0 60 129 62 887.3 0 62 130 163 887.3 0 163 131 89 887.3 0 89 132 57 887.3 0 57 133 52 887.3 0 52 134 46 887.3 0 46 135 99 887.3 0 99 136 41 887.3 0 41 137 35 887.3 0 35 138 68 887.3 0 68 139 66 887.3 0 66 140 87 887.3 0 87 141 89 887.3 0 89 142 96 887.3 0 96 143 84 887.3 0 84 144 106 887.3 0 106 145 77 887.3 0 77 146 112 887.3 0 112 147 71 887.3 0 71 148 53 887.3 0 53 149 84 887.3 0 84 150 74 887.3 0 74 151 67 887.3 0 67 152 87 887.3 0 87 153 81 887.3 0 81 154 76 887.3 0 76 155 106 887.3 0 106 156 90 887.3 0 90 157 171 887.3 0 171 158 140 887.3 0 140 159 157 887.3 0 157 160 60 887.3 0 60 161 63 887.3 0 63 162 59 887.3 0 59 163 65 887.3 0 65 164 59 887.3 0 59 165 85 887.3 0 85 166 57 887.3 0 57 167 73 887.3 0 73 168 90 887.3 0 90 169 108 887.3 0 108 170 97 887.3 0 97 171 107 887.3 0 107 172 84 887.3 0 84 173 80 887.3 0 80 174 72 887.3 0 72 175 105 887.3 0 105 176 137 887.3 0 137 177 224 887.3 0 224 178 223 887.3 0 223 179 305 887.3 0 305 180 353 887.3 0 353 181 449 887.3 0 449 182 3398 887.3 0 3398 183 8460 887.3 0 8460 184 3500 887.3 0 3500 185 158 887.3 0 158 186 152 887.3 0 152 187 129 887.3 0 129 188 320 887.3 0 320 189 345 887.3 0 345 190 377 887.3 0 377 191 414 887.3 0 414 192 679 887.3 0 679 193 8981 887.3 0 8981 194 6918 887.3 0 6918 195 14448 887.3 0 14448 196 5172 887.3 0 5172 197 2994 887.3 0 2994 198 706 887.3 0 706 199 85 887.3 0 85 200 139 887.3 0 139 201 97 887.3 0 97 202 74 887.3 0 74 203 70 887.3 0 70 204 73 887.3 0 73 205 128 887.3 0 128 206 95 887.3 0 95 207 135 887.3 0 135 208 127 887.3 0 127 209 121 887.3 0 121 210 86 887.3 0 86 211 102 887.3 0 102 212 100 887.3 0 100 213 497 887.3 0 497 214 1131 887.3 0 1131 215 965 887.3 0 965 216 894 887.3 0 894 217 466 887.3 0 466 218 789 887.3 0 789 219 1564 887.3 0 1564 220 1162 887.3 0 1162 221 844 887.3 0 844 222 291 887.3 0 291 223 164 887.3 0 164 224 73 887.3 0 73 225 105 887.3 0 105 226 103 887.3 0 103 227 120 887.3 0 120 228 132 887.3 0 132 229 84 887.3 0 84 230 95 887.3 0 95 231 168 887.3 0 168 232 175 887.3 0 175 233 171 887.3 0 171 234 143 887.3 0 143 235 125 887.3 0 125 236 183 887.3 0 183 237 208 887.3 0 208 238 297 887.3 0 297 239 1128 887.3 0 1128 240 1898 887.3 0 1898 241 1129 887.3 0 1129 242 566 887.3 0 566 243 65 887.3 0 65 244 103 887.3 0 103 245 96 887.3 0 96 246 155 887.3 0 155 247 133 887.3 0 133 248 190 887.3 0 190 249 230 887.3 0 230 250 943 887.3 0 943 251 1358 887.3 0 1358 252 1622 887.3 0 1622 253 749 887.3 0 749 254 76 887.3 0 76 255 130 887.3 0 130 256 154 887.3 0 154 257 235 887.3 0 235 258 288 887.3 0 288 259 1256 887.3 0 1256 260 1053 887.3 0 1053 261 625 887.3 0 625 262 206 887.3 0 206 263 1305 887.3 0 1305 264 1298 887.3 0 1298 265 1657 887.3 0 1657 266 1160 887.3 0 1160 267 1468 887.3 0 1468 268 512 887.3 0 512 269 350 887.3 0 350 270 425 887.3 0 425 271 175 887.3 0 175 272 121 887.3 0 121 273 89 887.3 0 89 274 113 887.3 0 113 275 146 887.3 0 146 276 187 887.3 0 187 277 142 887.3 0 142 278 117 887.3 0 117 279 136 887.3 0 136 280 147 887.3 0 147 281 176 887.3 0 176 282 184 887.3 0 184 283 321 887.3 0 321 284 269 887.3 0 269 285 277 887.3 0 277 286 307 887.3 0 307 287 353 887.3 0 353 288 3139 887.3 0 3139 289 4208 887.3 0 4208 290 2680 887.3 0 2680 291 946 887.3 0 946 292 176 887.3 0 176 293 270 887.3 0 270 294 330 887.3 0 330 295 264 887.3 0 264 296 167 887.3 0 167 297 263 887.3 0 263 298 263 887.3 0 263 299 264 887.3 0 264 300 565 887.3 0 565 301 246 887.3 0 246 Finished in 41.06 s (64 us/read; 0.94 M reads/minute). === Summary === Total reads processed: 643,519 Reads with adapters: 71,844 (11.2%) Reads that were too short: 1,641 (0.3%) Reads written (passing filters): 70,203 (10.9%) Total basepairs processed: 161,295,636 bp Total written (filtered): 18,388,393 bp (11.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 71844 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 7 0.0 2 0 0 0 7 15 28 0.0 3 0 0 20 8 16 485 0.0 3 0 410 50 25 17 62115 0.0 3 58874 2732 424 85 18 200 0.0 3 12 165 16 7 19 137 0.0 3 0 0 81 56 20 101 0.0 3 0 0 1 100 22 2 0.0 3 0 0 0 2 24 1 0.0 3 1 25 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 27 1 0.0 3 1 28 5 0.0 3 3 0 0 2 29 18 0.0 3 0 0 0 18 31 6 0.0 3 0 0 0 6 32 1 0.0 3 0 0 0 1 33 2 0.0 3 1 0 0 1 34 17 0.0 3 0 0 0 17 35 7 0.0 3 0 0 0 7 36 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 44 2 0.0 3 0 0 0 2 45 1 0.0 3 0 0 0 1 46 40 0.0 3 0 0 0 40 47 4 0.0 3 0 0 0 4 48 1 0.0 3 0 0 0 1 49 3 0.0 3 0 0 0 3 51 21 0.0 3 0 1 0 20 52 4 0.0 3 0 0 0 4 53 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 55 17 0.0 3 0 0 0 17 56 4 0.0 3 0 0 0 4 57 2 0.0 3 0 0 0 2 58 1 0.0 3 1 59 1 0.0 3 0 0 0 1 60 2 0.0 3 2 63 3 0.0 3 0 0 0 3 64 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 69 2 0.0 3 0 0 0 2 71 5 0.0 3 0 0 0 5 72 4 0.0 3 0 0 0 4 73 390 0.0 3 0 0 1 389 74 22 0.0 3 0 0 0 22 75 3 0.0 3 0 0 0 3 76 1 0.0 3 0 0 0 1 78 3 0.0 3 0 0 1 2 79 2 0.0 3 0 0 0 2 80 4 0.0 3 0 0 0 4 81 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 87 4 0.0 3 0 0 0 4 88 5 0.0 3 0 0 0 5 89 1 0.0 3 0 0 0 1 90 27 0.0 3 0 0 0 27 91 14 0.0 3 0 0 0 14 92 28 0.0 3 0 0 0 28 93 17 0.0 3 0 1 2 14 94 5 0.0 3 0 0 0 5 95 1 0.0 3 0 0 0 1 96 2 0.0 3 0 0 0 2 97 28 0.0 3 0 0 0 28 98 4 0.0 3 0 0 0 4 99 7 0.0 3 0 0 0 7 100 3 0.0 3 0 0 0 3 101 4 0.0 3 0 0 0 4 102 47 0.0 3 0 0 0 47 103 11 0.0 3 0 0 0 11 104 5 0.0 3 0 0 1 4 105 5 0.0 3 0 0 0 5 106 4 0.0 3 0 0 0 4 107 3 0.0 3 0 0 0 3 108 7 0.0 3 0 0 0 7 110 2 0.0 3 0 0 0 2 111 2 0.0 3 0 0 0 2 112 3 0.0 3 0 0 0 3 114 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 116 4 0.0 3 0 0 0 4 117 16 0.0 3 0 0 1 15 118 6 0.0 3 0 0 0 6 119 8 0.0 3 0 0 0 8 120 8 0.0 3 0 0 0 8 121 9 0.0 3 0 0 0 9 122 2707 0.0 3 0 0 26 2681 123 475 0.0 3 0 0 3 472 124 158 0.0 3 0 0 1 157 125 351 0.0 3 0 0 15 336 126 74 0.0 3 0 0 0 74 127 33 0.0 3 0 0 0 33 128 103 0.0 3 0 0 0 103 129 15 0.0 3 0 0 0 15 130 24 0.0 3 0 0 1 23 131 47 0.0 3 0 0 2 45 132 2 0.0 3 0 0 1 1 133 11 0.0 3 0 0 0 11 134 42 0.0 3 0 0 0 42 135 10 0.0 3 0 0 0 10 136 9 0.0 3 0 0 0 9 137 66 0.0 3 0 0 2 64 138 39 0.0 3 0 0 1 38 139 38 0.0 3 0 0 0 38 140 36 0.0 3 0 0 1 35 141 16 0.0 3 0 0 0 16 142 328 0.0 3 0 0 5 323 143 46 0.0 3 0 0 0 46 144 7 0.0 3 0 0 0 7 145 57 0.0 3 0 0 2 55 146 47 0.0 3 0 0 2 45 147 7 0.0 3 0 0 0 7 148 65 0.0 3 0 0 0 65 149 30 0.0 3 0 0 1 29 150 12 0.0 3 0 0 0 12 151 567 0.0 3 0 0 7 560 152 36 0.0 3 0 0 0 36 153 60 0.0 3 0 0 1 59 154 98 0.0 3 0 0 3 95 155 5 0.0 3 0 0 0 5 156 9 0.0 3 0 0 0 9 157 3 0.0 3 0 0 0 3 158 2 0.0 3 0 0 0 2 159 1 0.0 3 0 0 0 1 160 7 0.0 3 0 0 0 7 161 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 163 12 0.0 3 0 0 0 12 164 5 0.0 3 0 0 0 5 165 18 0.0 3 0 0 0 18 166 26 0.0 3 0 0 1 25 167 10 0.0 3 0 0 0 10 168 27 0.0 3 0 0 1 26 169 7 0.0 3 0 0 0 7 170 78 0.0 3 0 0 0 78 171 312 0.0 3 0 0 1 311 172 260 0.0 3 0 0 0 260 173 43 0.0 3 0 0 1 42 174 57 0.0 3 0 0 2 55 175 37 0.0 3 0 0 1 36 176 5 0.0 3 0 0 0 5 177 34 0.0 3 0 0 0 34 178 7 0.0 3 0 0 0 7 179 4 0.0 3 0 0 0 4 180 2 0.0 3 0 0 0 2 181 2 0.0 3 0 0 0 2 182 4 0.0 3 0 0 0 4 183 4 0.0 3 0 0 0 4 184 4 0.0 3 0 0 0 4 185 2 0.0 3 0 0 0 2 186 2 0.0 3 0 0 0 2 187 2 0.0 3 0 0 0 2 188 8 0.0 3 0 0 0 8 189 6 0.0 3 0 0 0 6 190 8 0.0 3 0 0 0 8 192 3 0.0 3 0 0 0 3 193 33 0.0 3 0 0 1 32 194 4 0.0 3 0 0 0 4 195 5 0.0 3 0 0 0 5 196 2 0.0 3 0 0 0 2 197 10 0.0 3 0 0 0 10 198 6 0.0 3 0 0 1 5 200 2 0.0 3 0 0 0 2 201 2 0.0 3 0 0 0 2 202 51 0.0 3 0 0 0 51 203 8 0.0 3 0 0 1 7 204 7 0.0 3 0 0 0 7 205 4 0.0 3 0 0 0 4 206 6 0.0 3 0 0 0 6 207 3 0.0 3 0 0 1 2 208 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 210 2 0.0 3 0 0 0 2 211 2 0.0 3 0 0 0 2 212 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 215 4 0.0 3 0 0 0 4 216 2 0.0 3 0 0 1 1 217 3 0.0 3 0 0 0 3 218 1 0.0 3 0 0 0 1 219 4 0.0 3 0 0 0 4 220 2 0.0 3 0 0 0 2 221 7 0.0 3 0 0 0 7 222 15 0.0 3 0 0 0 15 223 6 0.0 3 0 0 0 6 224 2 0.0 3 0 0 0 2 225 9 0.0 3 0 0 0 9 226 2 0.0 3 0 0 1 1 227 2 0.0 3 0 0 0 2 229 3 0.0 3 0 0 0 3 230 1 0.0 3 0 0 0 1 231 13 0.0 3 0 0 0 13 232 19 0.0 3 0 0 0 19 233 3 0.0 3 0 0 0 3 234 92 0.0 3 0 0 1 91 235 58 0.0 3 0 0 1 57 236 54 0.0 3 0 0 1 53 237 62 0.0 3 0 0 4 58 238 66 0.0 3 0 0 2 64 239 35 0.0 3 0 0 0 35 240 18 0.0 3 0 0 1 17 241 6 0.0 3 0 0 0 6 242 63 0.0 3 0 0 0 63 243 71 0.0 3 0 0 6 65 244 102 0.0 3 0 0 8 94 245 12 0.0 3 0 0 0 12 246 10 0.0 3 0 0 1 9 247 14 0.0 3 0 0 0 14 248 7 0.0 3 0 0 0 7 249 6 0.0 3 0 0 0 6 250 21 0.0 3 0 0 0 21 251 10 0.0 3 0 0 0 10 252 79 0.0 3 0 0 1 78 254 4 0.0 3 0 0 0 4 257 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 262 18 0.0 3 0 0 0 18 263 13 0.0 3 0 0 0 13 264 7 0.0 3 0 0 0 7 265 7 0.0 3 0 0 1 6 266 6 0.0 3 0 0 1 5 267 5 0.0 3 0 0 1 4 268 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 271 8 0.0 3 0 0 0 8 272 22 0.0 3 0 0 0 22 273 22 0.0 3 0 0 2 20 274 3 0.0 3 0 0 0 3 275 4 0.0 3 0 0 0 4 278 1 0.0 3 0 0 0 1 279 12 0.0 3 0 0 0 12 280 7 0.0 3 0 0 0 7 281 30 0.0 3 0 0 1 29 282 3 0.0 3 0 0 1 2 283 2 0.0 3 0 0 0 2 285 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.92 s (56 us/read; 1.07 M reads/minute). === Summary === Total reads processed: 70,203 Reads with adapters: 566 (0.8%) Reads written (passing filters): 70,203 (100.0%) Total basepairs processed: 18,388,393 bp Total written (filtered): 18,386,668 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 566 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 29.9% C: 55.5% G: 11.1% T: 3.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 548 1096.9 0 548 4 11 274.2 0 11 5 5 68.6 0 5 6 2 17.1 0 2