This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 173.68 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 371,635 (10.2%) Reads that were too short: 28,504 (0.8%) Reads written (passing filters): 343,131 (9.4%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 82,607,761 bp (7.6%) === Adapter 1 === Sequence: AAGGTC; Type: regular 5'; Length: 6; Trimmed: 371635 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 6574 887.3 0 6574 7 60276 887.3 0 60276 8 1961 887.3 0 1961 9 66521 887.3 0 66521 10 127 887.3 0 127 11 99 887.3 0 99 12 104 887.3 0 104 13 86 887.3 0 86 14 77 887.3 0 77 15 97 887.3 0 97 16 94 887.3 0 94 17 106 887.3 0 106 18 101 887.3 0 101 19 99 887.3 0 99 20 127 887.3 0 127 21 123 887.3 0 123 22 80 887.3 0 80 23 90 887.3 0 90 24 94 887.3 0 94 25 79 887.3 0 79 26 59 887.3 0 59 27 87 887.3 0 87 28 83 887.3 0 83 29 68 887.3 0 68 30 84 887.3 0 84 31 75 887.3 0 75 32 78 887.3 0 78 33 82 887.3 0 82 34 71 887.3 0 71 35 107 887.3 0 107 36 119 887.3 0 119 37 100 887.3 0 100 38 67 887.3 0 67 39 87 887.3 0 87 40 112 887.3 0 112 41 99 887.3 0 99 42 153 887.3 0 153 43 131 887.3 0 131 44 89 887.3 0 89 45 103 887.3 0 103 46 115 887.3 0 115 47 86 887.3 0 86 48 78 887.3 0 78 49 90 887.3 0 90 50 52 887.3 0 52 51 83 887.3 0 83 52 76 887.3 0 76 53 172 887.3 0 172 54 130 887.3 0 130 55 140 887.3 0 140 56 174 887.3 0 174 57 125 887.3 0 125 58 63 887.3 0 63 59 77 887.3 0 77 60 100 887.3 0 100 61 100 887.3 0 100 62 212 887.3 0 212 63 140 887.3 0 140 64 108 887.3 0 108 65 79 887.3 0 79 66 139 887.3 0 139 67 68 887.3 0 68 68 100 887.3 0 100 69 155 887.3 0 155 70 159 887.3 0 159 71 160 887.3 0 160 72 261 887.3 0 261 73 706 887.3 0 706 74 324 887.3 0 324 75 267 887.3 0 267 76 417 887.3 0 417 77 270 887.3 0 270 78 326 887.3 0 326 79 496 887.3 0 496 80 660 887.3 0 660 81 763 887.3 0 763 82 841 887.3 0 841 83 1498 887.3 0 1498 84 1661 887.3 0 1661 85 2923 887.3 0 2923 86 58716 887.3 0 58716 87 76727 887.3 0 76727 88 23636 887.3 0 23636 89 144 887.3 0 144 90 85 887.3 0 85 91 73 887.3 0 73 92 58 887.3 0 58 93 61 887.3 0 61 94 66 887.3 0 66 95 73 887.3 0 73 96 92 887.3 0 92 97 74 887.3 0 74 98 66 887.3 0 66 99 53 887.3 0 53 100 68 887.3 0 68 101 63 887.3 0 63 102 75 887.3 0 75 103 197 887.3 0 197 104 120 887.3 0 120 105 126 887.3 0 126 106 44 887.3 0 44 107 56 887.3 0 56 108 70 887.3 0 70 109 52 887.3 0 52 110 73 887.3 0 73 111 70 887.3 0 70 112 56 887.3 0 56 113 55 887.3 0 55 114 74 887.3 0 74 115 79 887.3 0 79 116 68 887.3 0 68 117 75 887.3 0 75 118 63 887.3 0 63 119 81 887.3 0 81 120 100 887.3 0 100 121 75 887.3 0 75 122 112 887.3 0 112 123 348 887.3 0 348 124 909 887.3 0 909 125 997 887.3 0 997 126 581 887.3 0 581 127 207 887.3 0 207 128 93 887.3 0 93 129 115 887.3 0 115 130 130 887.3 0 130 131 158 887.3 0 158 132 114 887.3 0 114 133 101 887.3 0 101 134 137 887.3 0 137 135 121 887.3 0 121 136 113 887.3 0 113 137 129 887.3 0 129 138 289 887.3 0 289 139 137 887.3 0 137 140 147 887.3 0 147 141 279 887.3 0 279 142 343 887.3 0 343 143 399 887.3 0 399 144 4901 887.3 0 4901 145 4784 887.3 0 4784 146 2070 887.3 0 2070 147 502 887.3 0 502 148 105 887.3 0 105 149 212 887.3 0 212 150 216 887.3 0 216 151 158 887.3 0 158 152 79 887.3 0 79 153 93 887.3 0 93 154 102 887.3 0 102 155 103 887.3 0 103 156 101 887.3 0 101 157 70 887.3 0 70 158 84 887.3 0 84 159 80 887.3 0 80 160 78 887.3 0 78 161 88 887.3 0 88 162 85 887.3 0 85 163 88 887.3 0 88 164 94 887.3 0 94 165 113 887.3 0 113 166 462 887.3 0 462 167 1168 887.3 0 1168 168 779 887.3 0 779 169 87 887.3 0 87 170 91 887.3 0 91 171 132 887.3 0 132 172 160 887.3 0 160 173 82 887.3 0 82 174 86 887.3 0 86 175 107 887.3 0 107 176 89 887.3 0 89 177 85 887.3 0 85 178 77 887.3 0 77 179 78 887.3 0 78 180 99 887.3 0 99 181 116 887.3 0 116 182 119 887.3 0 119 183 102 887.3 0 102 184 127 887.3 0 127 185 582 887.3 0 582 186 711 887.3 0 711 187 1083 887.3 0 1083 188 192 887.3 0 192 189 86 887.3 0 86 190 71 887.3 0 71 191 97 887.3 0 97 192 107 887.3 0 107 193 180 887.3 0 180 194 147 887.3 0 147 195 171 887.3 0 171 196 116 887.3 0 116 197 94 887.3 0 94 198 100 887.3 0 100 199 113 887.3 0 113 200 119 887.3 0 119 201 124 887.3 0 124 202 128 887.3 0 128 203 151 887.3 0 151 204 185 887.3 0 185 205 223 887.3 0 223 206 3533 887.3 0 3533 207 4377 887.3 0 4377 208 2182 887.3 0 2182 209 127 887.3 0 127 210 121 887.3 0 121 211 480 887.3 0 480 212 909 887.3 0 909 213 585 887.3 0 585 214 104 887.3 0 104 215 67 887.3 0 67 216 71 887.3 0 71 217 63 887.3 0 63 218 60 887.3 0 60 219 82 887.3 0 82 220 120 887.3 0 120 221 91 887.3 0 91 222 83 887.3 0 83 223 72 887.3 0 72 224 107 887.3 0 107 225 98 887.3 0 98 226 140 887.3 0 140 227 163 887.3 0 163 228 107 887.3 0 107 229 93 887.3 0 93 230 59 887.3 0 59 231 92 887.3 0 92 232 86 887.3 0 86 233 82 887.3 0 82 234 67 887.3 0 67 235 73 887.3 0 73 236 71 887.3 0 71 237 76 887.3 0 76 238 92 887.3 0 92 239 108 887.3 0 108 240 106 887.3 0 106 241 207 887.3 0 207 242 196 887.3 0 196 243 213 887.3 0 213 244 132 887.3 0 132 245 173 887.3 0 173 246 222 887.3 0 222 247 91 887.3 0 91 248 101 887.3 0 101 249 70 887.3 0 70 250 87 887.3 0 87 251 125 887.3 0 125 252 81 887.3 0 81 253 74 887.3 0 74 254 69 887.3 0 69 255 170 887.3 0 170 256 163 887.3 0 163 257 175 887.3 0 175 258 240 887.3 0 240 259 1701 887.3 0 1701 260 1885 887.3 0 1885 261 1528 887.3 0 1528 262 249 887.3 0 249 263 83 887.3 0 83 264 103 887.3 0 103 265 88 887.3 0 88 266 121 887.3 0 121 267 208 887.3 0 208 268 203 887.3 0 203 269 162 887.3 0 162 270 82 887.3 0 82 271 58 887.3 0 58 272 81 887.3 0 81 273 88 887.3 0 88 274 125 887.3 0 125 275 162 887.3 0 162 276 250 887.3 0 250 277 120 887.3 0 120 278 76 887.3 0 76 279 129 887.3 0 129 280 130 887.3 0 130 281 208 887.3 0 208 282 225 887.3 0 225 283 111 887.3 0 111 284 75 887.3 0 75 285 55 887.3 0 55 286 64 887.3 0 64 287 61 887.3 0 61 288 80 887.3 0 80 289 83 887.3 0 83 290 71 887.3 0 71 291 78 887.3 0 78 292 55 887.3 0 55 293 94 887.3 0 94 294 173 887.3 0 173 295 157 887.3 0 157 296 109 887.3 0 109 297 89 887.3 0 89 298 113 887.3 0 113 299 106 887.3 0 106 300 161 887.3 0 161 301 681 887.3 0 681 Finished in 22.40 s (65 us/read; 0.92 M reads/minute). === Summary === Total reads processed: 343,131 Reads with adapters: 73,599 (21.4%) Reads that were too short: 536 (0.2%) Reads written (passing filters): 73,063 (21.3%) Total basepairs processed: 82,607,761 bp Total written (filtered): 19,692,565 bp (23.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 73599 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 34 0.0 3 0 0 22 12 16 537 0.0 3 0 500 23 14 17 68179 0.0 3 64592 3040 448 99 18 186 0.0 3 12 156 9 9 19 1574 0.0 3 1399 64 71 40 20 69 0.0 3 0 5 0 64 21 17 0.0 3 0 0 2 15 22 4 0.0 3 0 0 0 4 23 1 0.0 3 0 0 1 24 5 0.0 3 1 0 0 4 28 1 0.0 3 1 29 1 0.0 3 1 31 2 0.0 3 0 0 1 1 34 1 0.0 3 0 0 0 1 36 2 0.0 3 1 0 0 1 37 19 0.0 3 0 0 1 18 38 1 0.0 3 0 0 0 1 43 2 0.0 3 0 0 0 2 46 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 5 0.0 3 0 0 0 5 50 1 0.0 3 0 0 0 1 51 8 0.0 3 0 0 0 8 54 5 0.0 3 0 0 0 5 59 5 0.0 3 0 0 0 5 61 2 0.0 3 0 0 0 2 62 2 0.0 3 0 0 0 2 66 26 0.0 3 0 0 0 26 68 18 0.0 3 0 0 0 18 69 2 0.0 3 0 0 0 2 71 4 0.0 3 0 0 0 4 72 4 0.0 3 0 0 0 4 73 1 0.0 3 0 0 0 1 74 2 0.0 3 0 0 0 2 79 1 0.0 3 0 0 0 1 80 25 0.0 3 0 0 0 25 81 3 0.0 3 0 0 0 3 83 6 0.0 3 0 0 0 6 84 3 0.0 3 0 0 0 3 85 6 0.0 3 0 0 0 6 87 3 0.0 3 0 0 0 3 88 5 0.0 3 0 0 0 5 92 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 1 97 2 0.0 3 0 0 0 2 99 4 0.0 3 0 0 0 4 100 2 0.0 3 0 0 0 2 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 104 107 0.0 3 0 0 0 107 105 1863 0.0 3 0 0 11 1852 106 108 0.0 3 0 0 0 108 107 3 0.0 3 0 0 0 3 108 168 0.0 3 0 0 1 167 109 19 0.0 3 0 0 3 16 110 2 0.0 3 0 0 0 2 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 1 119 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 3 0.0 3 0 0 0 3 131 27 0.0 3 0 0 1 26 132 4 0.0 3 0 0 1 3 133 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 138 3 0.0 3 0 0 0 3 139 3 0.0 3 0 0 0 3 140 6 0.0 3 0 0 0 6 142 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 153 5 0.0 3 0 0 0 5 155 4 0.0 3 0 0 0 4 156 41 0.0 3 0 0 0 41 157 8 0.0 3 0 0 0 8 158 11 0.0 3 0 0 0 11 160 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 164 7 0.0 3 0 0 0 7 165 15 0.0 3 0 0 0 15 166 111 0.0 3 0 0 2 109 167 2 0.0 3 0 0 0 2 168 4 0.0 3 0 0 0 4 169 3 0.0 3 0 0 0 3 170 3 0.0 3 0 0 0 3 173 2 0.0 3 0 0 0 2 174 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 178 2 0.0 3 0 0 0 2 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 0 2 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 4 0.0 3 0 0 0 4 193 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 195 1 0.0 3 0 0 0 1 196 4 0.0 3 0 0 1 3 197 8 0.0 3 0 0 0 8 198 1 0.0 3 0 0 0 1 199 3 0.0 3 0 0 0 3 200 2 0.0 3 0 0 1 1 202 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 7 0.0 3 0 0 0 7 206 83 0.0 3 0 0 1 82 207 55 0.0 3 0 0 1 54 208 6 0.0 3 0 0 0 6 212 3 0.0 3 0 0 0 3 214 3 0.0 3 0 0 0 3 215 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 218 2 0.0 3 0 0 0 2 219 7 0.0 3 0 0 1 6 221 11 0.0 3 0 0 0 11 223 2 0.0 3 0 0 1 1 225 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 7 0.0 3 0 0 0 7 282 3 0.0 3 0 0 0 3 283 4 0.0 3 0 0 0 4 284 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.16 s (57 us/read; 1.05 M reads/minute). === Summary === Total reads processed: 73,063 Reads with adapters: 1,733 (2.4%) Reads written (passing filters): 73,063 (100.0%) Total basepairs processed: 19,692,565 bp Total written (filtered): 19,687,263 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1733 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 64.7% C: 16.3% G: 16.2% T: 2.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1642 1141.6 0 1642 4 87 285.4 0 87 5 2 71.4 0 2 7 1 4.5 0 1 11 1 0.0 1 0 1