This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCTTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 172.19 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 215,889 (5.9%) Reads that were too short: 47,711 (1.3%) Reads written (passing filters): 168,178 (4.6%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 41,697,023 bp (3.8%) === Adapter 1 === Sequence: TCTTGG; Type: regular 5'; Length: 6; Trimmed: 215889 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 23645 887.3 0 23645 7 13957 887.3 0 13957 8 29610 887.3 0 29610 9 25190 887.3 0 25190 10 129 887.3 0 129 11 118 887.3 0 118 12 114 887.3 0 114 13 125 887.3 0 125 14 108 887.3 0 108 15 141 887.3 0 141 16 156 887.3 0 156 17 133 887.3 0 133 18 120 887.3 0 120 19 173 887.3 0 173 20 117 887.3 0 117 21 135 887.3 0 135 22 112 887.3 0 112 23 126 887.3 0 126 24 153 887.3 0 153 25 108 887.3 0 108 26 143 887.3 0 143 27 167 887.3 0 167 28 168 887.3 0 168 29 117 887.3 0 117 30 158 887.3 0 158 31 144 887.3 0 144 32 219 887.3 0 219 33 233 887.3 0 233 34 199 887.3 0 199 35 258 887.3 0 258 36 295 887.3 0 295 37 3367 887.3 0 3367 38 4349 887.3 0 4349 39 4923 887.3 0 4923 40 383 887.3 0 383 41 137 887.3 0 137 42 153 887.3 0 153 43 163 887.3 0 163 44 137 887.3 0 137 45 147 887.3 0 147 46 111 887.3 0 111 47 153 887.3 0 153 48 145 887.3 0 145 49 189 887.3 0 189 50 122 887.3 0 122 51 151 887.3 0 151 52 125 887.3 0 125 53 116 887.3 0 116 54 101 887.3 0 101 55 110 887.3 0 110 56 126 887.3 0 126 57 140 887.3 0 140 58 110 887.3 0 110 59 131 887.3 0 131 60 127 887.3 0 127 61 123 887.3 0 123 62 144 887.3 0 144 63 148 887.3 0 148 64 116 887.3 0 116 65 102 887.3 0 102 66 119 887.3 0 119 67 132 887.3 0 132 68 150 887.3 0 150 69 145 887.3 0 145 70 146 887.3 0 146 71 151 887.3 0 151 72 202 887.3 0 202 73 137 887.3 0 137 74 157 887.3 0 157 75 175 887.3 0 175 76 246 887.3 0 246 77 196 887.3 0 196 78 158 887.3 0 158 79 161 887.3 0 161 80 121 887.3 0 121 81 126 887.3 0 126 82 140 887.3 0 140 83 139 887.3 0 139 84 110 887.3 0 110 85 135 887.3 0 135 86 130 887.3 0 130 87 125 887.3 0 125 88 151 887.3 0 151 89 175 887.3 0 175 90 249 887.3 0 249 91 842 887.3 0 842 92 1100 887.3 0 1100 93 1404 887.3 0 1404 94 856 887.3 0 856 95 395 887.3 0 395 96 199 887.3 0 199 97 141 887.3 0 141 98 118 887.3 0 118 99 115 887.3 0 115 100 127 887.3 0 127 101 117 887.3 0 117 102 128 887.3 0 128 103 141 887.3 0 141 104 236 887.3 0 236 105 125 887.3 0 125 106 165 887.3 0 165 107 148 887.3 0 148 108 168 887.3 0 168 109 182 887.3 0 182 110 171 887.3 0 171 111 155 887.3 0 155 112 289 887.3 0 289 113 267 887.3 0 267 114 266 887.3 0 266 115 211 887.3 0 211 116 174 887.3 0 174 117 203 887.3 0 203 118 187 887.3 0 187 119 127 887.3 0 127 120 141 887.3 0 141 121 189 887.3 0 189 122 169 887.3 0 169 123 159 887.3 0 159 124 140 887.3 0 140 125 151 887.3 0 151 126 159 887.3 0 159 127 175 887.3 0 175 128 325 887.3 0 325 129 925 887.3 0 925 130 579 887.3 0 579 131 469 887.3 0 469 132 246 887.3 0 246 133 185 887.3 0 185 134 256 887.3 0 256 135 231 887.3 0 231 136 358 887.3 0 358 137 293 887.3 0 293 138 277 887.3 0 277 139 402 887.3 0 402 140 410 887.3 0 410 141 640 887.3 0 640 142 10644 887.3 0 10644 143 8199 887.3 0 8199 144 8456 887.3 0 8456 145 137 887.3 0 137 146 146 887.3 0 146 147 119 887.3 0 119 148 148 887.3 0 148 149 221 887.3 0 221 150 231 887.3 0 231 151 139 887.3 0 139 152 116 887.3 0 116 153 127 887.3 0 127 154 143 887.3 0 143 155 100 887.3 0 100 156 128 887.3 0 128 157 145 887.3 0 145 158 106 887.3 0 106 159 118 887.3 0 118 160 128 887.3 0 128 161 114 887.3 0 114 162 122 887.3 0 122 163 153 887.3 0 153 164 134 887.3 0 134 165 126 887.3 0 126 166 150 887.3 0 150 167 185 887.3 0 185 168 478 887.3 0 478 169 307 887.3 0 307 170 203 887.3 0 203 171 110 887.3 0 110 172 65 887.3 0 65 173 118 887.3 0 118 174 122 887.3 0 122 175 117 887.3 0 117 176 146 887.3 0 146 177 287 887.3 0 287 178 222 887.3 0 222 179 271 887.3 0 271 180 114 887.3 0 114 181 88 887.3 0 88 182 145 887.3 0 145 183 98 887.3 0 98 184 116 887.3 0 116 185 128 887.3 0 128 186 127 887.3 0 127 187 110 887.3 0 110 188 136 887.3 0 136 189 115 887.3 0 115 190 152 887.3 0 152 191 142 887.3 0 142 192 140 887.3 0 140 193 93 887.3 0 93 194 135 887.3 0 135 195 96 887.3 0 96 196 124 887.3 0 124 197 134 887.3 0 134 198 113 887.3 0 113 199 123 887.3 0 123 200 133 887.3 0 133 201 203 887.3 0 203 202 149 887.3 0 149 203 177 887.3 0 177 204 150 887.3 0 150 205 131 887.3 0 131 206 120 887.3 0 120 207 152 887.3 0 152 208 183 887.3 0 183 209 595 887.3 0 595 210 371 887.3 0 371 211 434 887.3 0 434 212 272 887.3 0 272 213 521 887.3 0 521 214 5503 887.3 0 5503 215 4570 887.3 0 4570 216 4360 887.3 0 4360 217 436 887.3 0 436 218 273 887.3 0 273 219 158 887.3 0 158 220 111 887.3 0 111 221 173 887.3 0 173 222 153 887.3 0 153 223 143 887.3 0 143 224 150 887.3 0 150 225 134 887.3 0 134 226 234 887.3 0 234 227 285 887.3 0 285 228 300 887.3 0 300 229 1118 887.3 0 1118 230 877 887.3 0 877 231 838 887.3 0 838 232 411 887.3 0 411 233 393 887.3 0 393 234 2810 887.3 0 2810 235 4450 887.3 0 4450 236 4497 887.3 0 4497 237 1661 887.3 0 1661 238 118 887.3 0 118 239 128 887.3 0 128 240 94 887.3 0 94 241 115 887.3 0 115 242 95 887.3 0 95 243 107 887.3 0 107 244 104 887.3 0 104 245 123 887.3 0 123 246 83 887.3 0 83 247 114 887.3 0 114 248 108 887.3 0 108 249 110 887.3 0 110 250 82 887.3 0 82 251 103 887.3 0 103 252 107 887.3 0 107 253 148 887.3 0 148 254 201 887.3 0 201 255 138 887.3 0 138 256 121 887.3 0 121 257 103 887.3 0 103 258 131 887.3 0 131 259 109 887.3 0 109 260 135 887.3 0 135 261 149 887.3 0 149 262 314 887.3 0 314 263 254 887.3 0 254 264 218 887.3 0 218 265 131 887.3 0 131 266 101 887.3 0 101 267 97 887.3 0 97 268 89 887.3 0 89 269 126 887.3 0 126 270 140 887.3 0 140 271 228 887.3 0 228 272 186 887.3 0 186 273 107 887.3 0 107 274 88 887.3 0 88 275 120 887.3 0 120 276 107 887.3 0 107 277 116 887.3 0 116 278 224 887.3 0 224 279 200 887.3 0 200 280 96 887.3 0 96 281 126 887.3 0 126 282 136 887.3 0 136 283 202 887.3 0 202 284 198 887.3 0 198 285 123 887.3 0 123 286 106 887.3 0 106 287 105 887.3 0 105 288 77 887.3 0 77 289 102 887.3 0 102 290 86 887.3 0 86 291 275 887.3 0 275 292 307 887.3 0 307 293 192 887.3 0 192 294 108 887.3 0 108 295 193 887.3 0 193 296 458 887.3 0 458 297 734 887.3 0 734 298 577 887.3 0 577 299 230 887.3 0 230 300 90 887.3 0 90 301 225 887.3 0 225 Finished in 11.46 s (68 us/read; 0.88 M reads/minute). === Summary === Total reads processed: 168,178 Reads with adapters: 52,062 (31.0%) Reads that were too short: 132 (0.1%) Reads written (passing filters): 51,930 (30.9%) Total basepairs processed: 41,697,023 bp Total written (filtered): 14,335,637 bp (34.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 52062 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 16 0.0 3 0 0 10 6 16 224 0.0 3 0 193 16 15 17 29563 0.0 3 27853 1429 219 62 18 21737 0.0 3 20615 929 154 39 19 130 0.0 3 16 54 39 21 20 103 0.0 3 2 0 35 66 21 21 0.0 3 0 1 0 20 22 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 38 1 0.0 3 1 39 1 0.0 3 0 0 0 1 41 1 0.0 3 1 42 4 0.0 3 0 0 0 4 46 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 28 0.0 3 0 0 1 27 53 3 0.0 3 0 0 0 3 54 3 0.0 3 0 0 0 3 55 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 66 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 74 2 0.0 3 0 0 0 2 75 2 0.0 3 0 0 1 1 76 3 0.0 3 2 0 0 1 78 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 81 4 0.0 3 0 0 0 4 82 2 0.0 3 0 0 0 2 83 3 0.0 3 0 0 0 3 85 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 99 4 0.0 3 0 0 0 4 100 1 0.0 3 0 0 0 1 101 7 0.0 3 0 0 0 7 102 2 0.0 3 0 0 0 2 103 3 0.0 3 0 0 0 3 104 3 0.0 3 0 0 0 3 108 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 110 3 0.0 3 0 0 0 3 115 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 5 0.0 3 0 0 0 5 130 6 0.0 3 0 0 0 6 132 4 0.0 3 0 0 0 4 133 12 0.0 3 0 0 0 12 135 1 0.0 3 0 0 0 1 136 4 0.0 3 0 0 0 4 142 2 0.0 3 0 0 0 2 145 2 0.0 3 0 0 0 2 149 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 151 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 154 3 0.0 3 0 0 0 3 155 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 157 5 0.0 3 0 0 0 5 158 1 0.0 3 0 0 1 164 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 1 171 1 0.0 3 0 0 0 1 176 2 0.0 3 0 0 0 2 178 1 0.0 3 0 0 0 1 180 4 0.0 3 0 0 0 4 181 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 3 0.0 3 0 0 1 2 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 193 6 0.0 3 0 0 0 6 194 2 0.0 3 0 0 0 2 197 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 201 2 0.0 3 0 0 0 2 203 1 0.0 3 0 0 0 1 204 2 0.0 3 0 0 0 2 205 2 0.0 3 0 0 1 1 206 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 214 3 0.0 3 0 0 0 3 215 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 218 25 0.0 3 0 0 3 22 221 2 0.0 3 0 0 0 2 222 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 238 3 0.0 3 0 0 0 3 246 2 0.0 3 0 0 0 2 250 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 256 4 0.0 3 0 0 1 3 260 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 5 0.0 3 0 0 0 5 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.97 s (57 us/read; 1.05 M reads/minute). === Summary === Total reads processed: 51,930 Reads with adapters: 542 (1.0%) Reads written (passing filters): 51,930 (100.0%) Total basepairs processed: 14,335,637 bp Total written (filtered): 14,333,975 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 542 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 19.2% C: 61.1% G: 16.4% T: 3.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 518 811.4 0 518 4 17 202.9 0 17 5 5 50.7 0 5 6 1 12.7 0 1 9 1 0.2 0 0 1