This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTACAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 175.09 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 76,272 (2.1%) Reads that were too short: 12,595 (0.3%) Reads written (passing filters): 63,677 (1.8%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 16,594,497 bp (1.5%) === Adapter 1 === Sequence: GTACAC; Type: regular 5'; Length: 6; Trimmed: 76272 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1124 887.3 0 1124 7 26394 887.3 0 26394 8 378 887.3 0 378 9 19498 887.3 0 19498 10 36 887.3 0 36 11 19 887.3 0 19 12 16 887.3 0 16 13 21 887.3 0 21 14 13 887.3 0 13 15 142 887.3 0 142 16 15 887.3 0 15 17 20 887.3 0 20 18 11 887.3 0 11 19 7 887.3 0 7 20 20 887.3 0 20 21 14 887.3 0 14 22 22 887.3 0 22 23 26 887.3 0 26 24 16 887.3 0 16 25 13 887.3 0 13 26 17 887.3 0 17 27 8 887.3 0 8 28 25 887.3 0 25 29 24 887.3 0 24 30 11 887.3 0 11 31 29 887.3 0 29 32 27 887.3 0 27 33 27 887.3 0 27 34 37 887.3 0 37 35 38 887.3 0 38 36 24 887.3 0 24 37 18 887.3 0 18 38 24 887.3 0 24 39 28 887.3 0 28 40 42 887.3 0 42 41 26 887.3 0 26 42 47 887.3 0 47 43 23 887.3 0 23 44 24 887.3 0 24 45 27 887.3 0 27 46 27 887.3 0 27 47 24 887.3 0 24 48 17 887.3 0 17 49 28 887.3 0 28 50 35 887.3 0 35 51 62 887.3 0 62 52 24 887.3 0 24 53 27 887.3 0 27 54 22 887.3 0 22 55 20 887.3 0 20 56 14 887.3 0 14 57 41 887.3 0 41 58 25 887.3 0 25 59 23 887.3 0 23 60 19 887.3 0 19 61 18 887.3 0 18 62 13 887.3 0 13 63 30 887.3 0 30 64 15 887.3 0 15 65 29 887.3 0 29 66 28 887.3 0 28 67 26 887.3 0 26 68 25 887.3 0 25 69 135 887.3 0 135 70 259 887.3 0 259 71 102 887.3 0 102 72 17 887.3 0 17 73 16 887.3 0 16 74 24 887.3 0 24 75 16 887.3 0 16 76 30 887.3 0 30 77 22 887.3 0 22 78 19 887.3 0 19 79 27 887.3 0 27 80 26 887.3 0 26 81 39 887.3 0 39 82 35 887.3 0 35 83 27 887.3 0 27 84 34 887.3 0 34 85 33 887.3 0 33 86 42 887.3 0 42 87 84 887.3 0 84 88 42 887.3 0 42 89 63 887.3 0 63 90 73 887.3 0 73 91 69 887.3 0 69 92 47 887.3 0 47 93 44 887.3 0 44 94 57 887.3 0 57 95 41 887.3 0 41 96 66 887.3 0 66 97 87 887.3 0 87 98 68 887.3 0 68 99 50 887.3 0 50 100 314 887.3 0 314 101 391 887.3 0 391 102 887 887.3 0 887 103 258 887.3 0 258 104 99 887.3 0 99 105 56 887.3 0 56 106 40 887.3 0 40 107 41 887.3 0 41 108 29 887.3 0 29 109 35 887.3 0 35 110 33 887.3 0 33 111 46 887.3 0 46 112 37 887.3 0 37 113 44 887.3 0 44 114 25 887.3 0 25 115 23 887.3 0 23 116 43 887.3 0 43 117 35 887.3 0 35 118 67 887.3 0 67 119 42 887.3 0 42 120 36 887.3 0 36 121 22 887.3 0 22 122 62 887.3 0 62 123 90 887.3 0 90 124 55 887.3 0 55 125 46 887.3 0 46 126 37 887.3 0 37 127 32 887.3 0 32 128 21 887.3 0 21 129 56 887.3 0 56 130 37 887.3 0 37 131 92 887.3 0 92 132 222 887.3 0 222 133 199 887.3 0 199 134 76 887.3 0 76 135 65 887.3 0 65 136 52 887.3 0 52 137 81 887.3 0 81 138 69 887.3 0 69 139 74 887.3 0 74 140 145 887.3 0 145 141 72 887.3 0 72 142 79 887.3 0 79 143 120 887.3 0 120 144 142 887.3 0 142 145 139 887.3 0 139 146 51 887.3 0 51 147 55 887.3 0 55 148 80 887.3 0 80 149 97 887.3 0 97 150 73 887.3 0 73 151 67 887.3 0 67 152 84 887.3 0 84 153 70 887.3 0 70 154 51 887.3 0 51 155 77 887.3 0 77 156 60 887.3 0 60 157 222 887.3 0 222 158 535 887.3 0 535 159 238 887.3 0 238 160 344 887.3 0 344 161 171 887.3 0 171 162 227 887.3 0 227 163 64 887.3 0 64 164 66 887.3 0 66 165 42 887.3 0 42 166 45 887.3 0 45 167 46 887.3 0 46 168 104 887.3 0 104 169 74 887.3 0 74 170 86 887.3 0 86 171 63 887.3 0 63 172 54 887.3 0 54 173 69 887.3 0 69 174 45 887.3 0 45 175 68 887.3 0 68 176 787 887.3 0 787 177 1246 887.3 0 1246 178 1270 887.3 0 1270 179 81 887.3 0 81 180 74 887.3 0 74 181 53 887.3 0 53 182 59 887.3 0 59 183 79 887.3 0 79 184 92 887.3 0 92 185 77 887.3 0 77 186 68 887.3 0 68 187 136 887.3 0 136 188 122 887.3 0 122 189 114 887.3 0 114 190 91 887.3 0 91 191 64 887.3 0 64 192 48 887.3 0 48 193 26 887.3 0 26 194 53 887.3 0 53 195 50 887.3 0 50 196 89 887.3 0 89 197 101 887.3 0 101 198 94 887.3 0 94 199 76 887.3 0 76 200 60 887.3 0 60 201 56 887.3 0 56 202 72 887.3 0 72 203 78 887.3 0 78 204 66 887.3 0 66 205 69 887.3 0 69 206 67 887.3 0 67 207 62 887.3 0 62 208 67 887.3 0 67 209 53 887.3 0 53 210 96 887.3 0 96 211 98 887.3 0 98 212 110 887.3 0 110 213 108 887.3 0 108 214 88 887.3 0 88 215 84 887.3 0 84 216 83 887.3 0 83 217 86 887.3 0 86 218 70 887.3 0 70 219 71 887.3 0 71 220 82 887.3 0 82 221 73 887.3 0 73 222 99 887.3 0 99 223 123 887.3 0 123 224 177 887.3 0 177 225 126 887.3 0 126 226 101 887.3 0 101 227 91 887.3 0 91 228 80 887.3 0 80 229 67 887.3 0 67 230 107 887.3 0 107 231 81 887.3 0 81 232 86 887.3 0 86 233 91 887.3 0 91 234 78 887.3 0 78 235 89 887.3 0 89 236 73 887.3 0 73 237 117 887.3 0 117 238 284 887.3 0 284 239 229 887.3 0 229 240 205 887.3 0 205 241 110 887.3 0 110 242 112 887.3 0 112 243 165 887.3 0 165 244 164 887.3 0 164 245 106 887.3 0 106 246 103 887.3 0 103 247 144 887.3 0 144 248 106 887.3 0 106 249 106 887.3 0 106 250 99 887.3 0 99 251 128 887.3 0 128 252 103 887.3 0 103 253 105 887.3 0 105 254 108 887.3 0 108 255 114 887.3 0 114 256 126 887.3 0 126 257 151 887.3 0 151 258 108 887.3 0 108 259 121 887.3 0 121 260 100 887.3 0 100 261 96 887.3 0 96 262 202 887.3 0 202 263 215 887.3 0 215 264 334 887.3 0 334 265 234 887.3 0 234 266 209 887.3 0 209 267 120 887.3 0 120 268 68 887.3 0 68 269 96 887.3 0 96 270 110 887.3 0 110 271 170 887.3 0 170 272 163 887.3 0 163 273 144 887.3 0 144 274 190 887.3 0 190 275 185 887.3 0 185 276 181 887.3 0 181 277 156 887.3 0 156 278 119 887.3 0 119 279 162 887.3 0 162 280 93 887.3 0 93 281 114 887.3 0 114 282 142 887.3 0 142 283 147 887.3 0 147 284 149 887.3 0 149 285 128 887.3 0 128 286 146 887.3 0 146 287 159 887.3 0 159 288 153 887.3 0 153 289 118 887.3 0 118 290 127 887.3 0 127 291 152 887.3 0 152 292 175 887.3 0 175 293 155 887.3 0 155 294 118 887.3 0 118 295 106 887.3 0 106 296 96 887.3 0 96 297 124 887.3 0 124 298 154 887.3 0 154 299 175 887.3 0 175 300 197 887.3 0 197 301 247 887.3 0 247 Finished in 4.91 s (77 us/read; 0.78 M reads/minute). === Summary === Total reads processed: 63,677 Reads with adapters: 27,767 (43.6%) Reads that were too short: 24 (0.0%) Reads written (passing filters): 27,743 (43.6%) Total basepairs processed: 16,594,497 bp Total written (filtered): 7,544,016 bp (45.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 27767 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 7 0.0 3 0 0 7 19 245 0.0 3 0 237 6 1 1 20 26259 0.0 4 25804 446 9 21 58 0.0 4 14 43 0 0 1 22 14 0.0 4 1 8 5 23 637 0.0 4 612 9 0 15 1 24 2 0.0 4 0 0 1 0 1 34 2 0.0 4 1 0 0 0 1 35 1 0.0 4 1 38 1 0.0 4 0 0 0 1 41 2 0.0 4 0 0 0 0 2 66 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 1 73 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 14 0.0 4 0 0 0 0 14 127 498 0.0 4 0 0 0 0 498 134 3 0.0 4 0 0 0 0 3 135 3 0.0 4 0 0 0 0 3 142 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 1 180 2 0.0 4 0 0 0 0 2 200 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 3 0.0 4 0 0 0 0 3 271 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.60 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 27,743 Reads with adapters: 601 (2.2%) Reads written (passing filters): 27,743 (100.0%) Total basepairs processed: 7,544,016 bp Total written (filtered): 7,532,990 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 601 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 22.6% C: 4.5% G: 68.2% T: 3.7% none/other: 1.0% Overview of removed sequences length count expect max.err error counts 3 75 433.5 0 75 4 2 108.4 0 2 5 2 27.1 0 2 9 70 0.1 0 2 68 10 30 0.0 1 0 30 11 278 0.0 1 1 277 12 50 0.0 1 0 50 13 1 0.0 1 0 1 15 1 0.0 1 0 1 20 67 0.0 1 56 11 21 5 0.0 1 3 2 152 1 0.0 1 0 1 225 12 0.0 1 11 1 226 1 0.0 1 1 274 6 0.0 1 6