This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 172.54 s (47 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 140,828 (3.9%) Reads that were too short: 67,869 (1.9%) Reads written (passing filters): 72,959 (2.0%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 19,398,577 bp (1.8%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 140828 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2022 887.3 0 2022 7 800 887.3 0 800 8 28202 887.3 0 28202 9 23401 887.3 0 23401 10 72 887.3 0 72 11 59 887.3 0 59 12 96 887.3 0 96 13 114 887.3 0 114 14 62 887.3 0 62 15 50 887.3 0 50 16 57 887.3 0 57 17 85 887.3 0 85 18 61 887.3 0 61 19 104 887.3 0 104 20 63 887.3 0 63 21 74 887.3 0 74 22 68 887.3 0 68 23 72 887.3 0 72 24 91 887.3 0 91 25 53 887.3 0 53 26 43 887.3 0 43 27 53 887.3 0 53 28 73 887.3 0 73 29 65 887.3 0 65 30 47 887.3 0 47 31 62 887.3 0 62 32 67 887.3 0 67 33 58 887.3 0 58 34 70 887.3 0 70 35 57 887.3 0 57 36 76 887.3 0 76 37 58 887.3 0 58 38 71 887.3 0 71 39 69 887.3 0 69 40 113 887.3 0 113 41 110 887.3 0 110 42 61 887.3 0 61 43 65 887.3 0 65 44 72 887.3 0 72 45 67 887.3 0 67 46 62 887.3 0 62 47 62 887.3 0 62 48 45 887.3 0 45 49 48 887.3 0 48 50 62 887.3 0 62 51 111 887.3 0 111 52 141 887.3 0 141 53 172 887.3 0 172 54 74 887.3 0 74 55 53 887.3 0 53 56 53 887.3 0 53 57 51 887.3 0 51 58 75 887.3 0 75 59 90 887.3 0 90 60 60 887.3 0 60 61 61 887.3 0 61 62 89 887.3 0 89 63 124 887.3 0 124 64 65 887.3 0 65 65 55 887.3 0 55 66 105 887.3 0 105 67 71 887.3 0 71 68 72 887.3 0 72 69 60 887.3 0 60 70 50 887.3 0 50 71 63 887.3 0 63 72 66 887.3 0 66 73 59 887.3 0 59 74 74 887.3 0 74 75 88 887.3 0 88 76 76 887.3 0 76 77 73 887.3 0 73 78 126 887.3 0 126 79 73 887.3 0 73 80 61 887.3 0 61 81 80 887.3 0 80 82 82 887.3 0 82 83 76 887.3 0 76 84 84 887.3 0 84 85 66 887.3 0 66 86 79 887.3 0 79 87 64 887.3 0 64 88 69 887.3 0 69 89 98 887.3 0 98 90 85 887.3 0 85 91 119 887.3 0 119 92 91 887.3 0 91 93 95 887.3 0 95 94 113 887.3 0 113 95 111 887.3 0 111 96 73 887.3 0 73 97 99 887.3 0 99 98 73 887.3 0 73 99 100 887.3 0 100 100 142 887.3 0 142 101 110 887.3 0 110 102 93 887.3 0 93 103 79 887.3 0 79 104 55 887.3 0 55 105 63 887.3 0 63 106 59 887.3 0 59 107 81 887.3 0 81 108 69 887.3 0 69 109 89 887.3 0 89 110 71 887.3 0 71 111 71 887.3 0 71 112 97 887.3 0 97 113 71 887.3 0 71 114 77 887.3 0 77 115 69 887.3 0 69 116 94 887.3 0 94 117 83 887.3 0 83 118 102 887.3 0 102 119 66 887.3 0 66 120 64 887.3 0 64 121 78 887.3 0 78 122 92 887.3 0 92 123 81 887.3 0 81 124 183 887.3 0 183 125 92 887.3 0 92 126 83 887.3 0 83 127 67 887.3 0 67 128 69 887.3 0 69 129 68 887.3 0 68 130 66 887.3 0 66 131 94 887.3 0 94 132 114 887.3 0 114 133 130 887.3 0 130 134 74 887.3 0 74 135 80 887.3 0 80 136 132 887.3 0 132 137 479 887.3 0 479 138 1288 887.3 0 1288 139 974 887.3 0 974 140 595 887.3 0 595 141 67 887.3 0 67 142 79 887.3 0 79 143 82 887.3 0 82 144 130 887.3 0 130 145 84 887.3 0 84 146 91 887.3 0 91 147 76 887.3 0 76 148 70 887.3 0 70 149 66 887.3 0 66 150 77 887.3 0 77 151 84 887.3 0 84 152 78 887.3 0 78 153 91 887.3 0 91 154 83 887.3 0 83 155 85 887.3 0 85 156 66 887.3 0 66 157 78 887.3 0 78 158 85 887.3 0 85 159 81 887.3 0 81 160 81 887.3 0 81 161 75 887.3 0 75 162 72 887.3 0 72 163 72 887.3 0 72 164 74 887.3 0 74 165 87 887.3 0 87 166 79 887.3 0 79 167 79 887.3 0 79 168 73 887.3 0 73 169 86 887.3 0 86 170 74 887.3 0 74 171 104 887.3 0 104 172 86 887.3 0 86 173 113 887.3 0 113 174 72 887.3 0 72 175 61 887.3 0 61 176 70 887.3 0 70 177 77 887.3 0 77 178 76 887.3 0 76 179 100 887.3 0 100 180 107 887.3 0 107 181 103 887.3 0 103 182 84 887.3 0 84 183 103 887.3 0 103 184 85 887.3 0 85 185 77 887.3 0 77 186 122 887.3 0 122 187 121 887.3 0 121 188 119 887.3 0 119 189 115 887.3 0 115 190 64 887.3 0 64 191 80 887.3 0 80 192 83 887.3 0 83 193 54 887.3 0 54 194 64 887.3 0 64 195 76 887.3 0 76 196 84 887.3 0 84 197 77 887.3 0 77 198 101 887.3 0 101 199 86 887.3 0 86 200 85 887.3 0 85 201 71 887.3 0 71 202 85 887.3 0 85 203 104 887.3 0 104 204 109 887.3 0 109 205 78 887.3 0 78 206 70 887.3 0 70 207 92 887.3 0 92 208 98 887.3 0 98 209 77 887.3 0 77 210 113 887.3 0 113 211 79 887.3 0 79 212 83 887.3 0 83 213 74 887.3 0 74 214 177 887.3 0 177 215 149 887.3 0 149 216 119 887.3 0 119 217 102 887.3 0 102 218 88 887.3 0 88 219 95 887.3 0 95 220 84 887.3 0 84 221 61 887.3 0 61 222 65 887.3 0 65 223 81 887.3 0 81 224 89 887.3 0 89 225 110 887.3 0 110 226 73 887.3 0 73 227 85 887.3 0 85 228 85 887.3 0 85 229 90 887.3 0 90 230 106 887.3 0 106 231 77 887.3 0 77 232 68 887.3 0 68 233 84 887.3 0 84 234 86 887.3 0 86 235 95 887.3 0 95 236 93 887.3 0 93 237 99 887.3 0 99 238 316 887.3 0 316 239 379 887.3 0 379 240 226 887.3 0 226 241 68 887.3 0 68 242 92 887.3 0 92 243 103 887.3 0 103 244 80 887.3 0 80 245 83 887.3 0 83 246 72 887.3 0 72 247 99 887.3 0 99 248 75 887.3 0 75 249 92 887.3 0 92 250 106 887.3 0 106 251 121 887.3 0 121 252 114 887.3 0 114 253 76 887.3 0 76 254 77 887.3 0 77 255 123 887.3 0 123 256 138 887.3 0 138 257 233 887.3 0 233 258 258 887.3 0 258 259 320 887.3 0 320 260 245 887.3 0 245 261 150 887.3 0 150 262 124 887.3 0 124 263 174 887.3 0 174 264 163 887.3 0 163 265 120 887.3 0 120 266 168 887.3 0 168 267 132 887.3 0 132 268 148 887.3 0 148 269 147 887.3 0 147 270 215 887.3 0 215 271 291 887.3 0 291 272 572 887.3 0 572 273 6482 887.3 0 6482 274 5895 887.3 0 5895 275 3416 887.3 0 3416 276 441 887.3 0 441 277 766 887.3 0 766 278 304 887.3 0 304 279 221 887.3 0 221 280 204 887.3 0 204 281 133 887.3 0 133 282 184 887.3 0 184 283 517 887.3 0 517 284 687 887.3 0 687 285 965 887.3 0 965 286 1821 887.3 0 1821 287 1643 887.3 0 1643 288 4193 887.3 0 4193 289 355 887.3 0 355 290 232 887.3 0 232 291 296 887.3 0 296 292 482 887.3 0 482 293 3418 887.3 0 3418 294 2425 887.3 0 2425 295 1253 887.3 0 1253 296 290 887.3 0 290 297 823 887.3 0 823 298 3151 887.3 0 3151 299 7888 887.3 0 7888 300 6350 887.3 0 6350 301 3811 887.3 0 3811 Finished in 5.44 s (75 us/read; 0.80 M reads/minute). === Summary === Total reads processed: 72,959 Reads with adapters: 28,329 (38.8%) Reads that were too short: 46 (0.1%) Reads written (passing filters): 28,283 (38.8%) Total basepairs processed: 19,398,577 bp Total written (filtered): 7,795,144 bp (40.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 28329 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 11 0.0 3 0 0 9 2 16 217 0.0 3 0 197 14 6 17 27797 0.0 3 26414 1174 176 33 18 87 0.0 3 6 73 6 2 19 64 0.0 3 6 0 37 21 20 27 0.0 3 4 1 1 21 21 2 0.0 3 1 0 0 1 23 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 35 3 0.0 3 0 1 0 2 36 1 0.0 3 0 0 1 43 2 0.0 3 0 0 1 1 45 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 55 35 0.0 3 0 0 1 34 56 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 4 0.0 3 0 0 2 2 64 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 2 0.0 3 0 0 0 2 98 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 106 5 0.0 3 0 0 0 5 108 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 129 3 0.0 3 0 0 0 3 132 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 149 2 0.0 3 0 0 0 2 151 2 0.0 3 0 0 0 2 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 194 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 3 0.0 3 0 0 0 3 283 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.64 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 28,283 Reads with adapters: 67 (0.2%) Reads written (passing filters): 28,283 (100.0%) Total basepairs processed: 7,795,144 bp Total written (filtered): 7,794,928 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 67 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.7% C: 25.4% G: 11.9% T: 11.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 57 441.9 0 57 4 5 110.5 0 5 5 5 27.6 0 5